Modulation of ROCK 1 expression

ABSTRACT

Compounds, compositions and methods are provided for modulating the expression of ROCK 1. The compositions comprise oligonucleotides, targeted to nucleic acid encoding ROCK 1. Methods of using these compounds for modulation of ROCK 1 expression and for diagnosis and treatment of disease associated with expression of ROCK 1 are provided.

FIELD OF THE INVENTION

[0001] The present invention provides compositions and methods formodulating the expression of ROCK 1. In particular, this inventionrelates to compounds, particularly oligonucleotide compounds, which, inpreferred embodiments, hybridize with nucleic acid molecules encodingROCK 1. Such compounds are shown herein to modulate the expression ofROCK 1.

BACKGROUND OF THE INVENTION

[0002] The Rho family of GTPases (Rho, Rac, and Cdc42) are Ras-relatedsmall GTP-binding proteins (p21^(ras) proteins) that regulatecoordinated changes in the actin cytoskeleton and the formation ofdistinct cytoskeletal structures. Controlled changes to the actincytoskeleton are vital for many cellular processes, including motility,adhesion, cell division, cell death and phagocytosis (Ridley, TrendsCell Biol, 2001, 11, 471-477).

[0003] ROCK 1, a kinase that is activated upon binding to GTP-bound formof Rho, was the first target shown to affect actin organization (Leunget al., Mol. Cell. Biol., 1996, 16, 5313-5327). The proposed mechanismby which Rho signals for changes in the actin cytoskeleton involvesphosphorylation of LIM-Kinase by ROCK 1, and LIM-Kinase in turnphosphorylates cofilin. Cofilin exhibits actin-depolymerizing activity,and this activity is inhibited upon phosphorylation of cofilin, thusROCK 1 is directly involved in the Rho-induced reorganization of thecytoskeleton (Maekawa et al., Science, 1999, 285, 895-898).

[0004] The gene encoding ROCK 1 (also called Rho-associatedcoiled-coil-containing protein kinase 1, p160ROCK, ROK alpha, and RhoKinase 1) was cloned in 1996 (Ishizaki et al., Embo J, 1996, 15,1885-1893). ROCK 1 has a Ser/Thr kinase domain at the N-terminus, acoiled-coil structure of approximately 600 amino acids, a cysteine-richzinc finger-like motif, and a pleckstrin homology region at theC-terminus.

[0005] The involvement of ROCK 1 in rearrangement of the cytoskeletonmay be relevant for tumor invasion and metastasis. ROCK 1 and RhoA areactivated by transforming growth factor-beta 1 (TGF-beta), a growthfactor whose signaling pathway has been implicated in the promotingtumor progression by stimulating epithelial-to-mesenchymaltransdifferentiation, and inhibition of ROCK 1 blocks actin cytoskeletonrearrangement induced by TGF-beta (Bhowmick et al., Mol. Biol. Cell,2001, 12, 27-36). ROCK 1 and Rho are also activated by the chemokineSDF-1 alpha, a pathway that leads to a polarized lymphocyte shape andchemotactic response (Vicente-Manzanares et al., J. Immunol., 2002, 168,400-410). Increased levels of ROCK 1 mRNA result from increasedproduction of HEF1, a cas-family protein implicated in oncogenictransformations, leading to a suggestion that this is a mechanismthrough which misregulation of HEF1 leads to cancer progression (Fashenaet al., J. Cell Sci., 2002, 115, 99-111).

[0006] ROCK 1 plays a critical role in the negative regulation of thesize and motility of axonal growth cones and inhibition of ROCK 1activity in neurons has been suggested as a therapeutic strategy fortreating axonopathies such as those induced by nerve injury or diabetes,or to promote axon regeneraton (Bito et al., Neuron, 2000, 26, 431-441).

[0007] Currently, there are no known therapeutic agents whicheffectively inhibit the synthesis of ROCK 1 and to date, investigativestrategies aimed at modulating ROCK 1 function have involved the use ofmutants, one small molecule inhibitor, and an antisense oligonucleotide.

[0008] Investigative strategies have employed mutants of ROCK 1 toelucidate the regions of ROCK 1 that are necessary to bind Rho (Fujisawaet al., J. Biol. Chem., 1996, 271, 23022-23028), and regions that arenecessary to induce focal adhesions and stress fibers (Ishizaki et al.,FEBS Lett., 1997, 404, 118-124).

[0009] The small molecule(+)-R-trans-4-(1-aminoethyl)-N-(4-pyridyl)cyclohexane carboxamidedihydrochloride, also known as Y-27632, has been used as an inhibitor ofROCK 1 kinase activity and helped elucidate the role of ROCK 1 in actinstress fiber assembly (Tominaga et al., Embo J, 1998, 17, 4712-4722),aggregation and adhesion of human polymorphonuclear leukocytes(Kawaguchi et al., Eur. J. Pharmacol., 2000, 403, 203-208), hypertrophicsignaling in cardiac myocytes (Kuwahara et al., FEBS Lett., 1999, 452,314-318), tight junction permeability (Hirase et al., J. Biol. Chem.,2001, 276, 10423-10431), gastrointestinal motility (Tomomasa et al.,Life Sci., 2000, 66, L29-34), embryonic morphogenesis (Wei et al.,Development, 2001, 128, 2953-2962), and angiogenesis (Uchida et al.,Biochem. Biophys. Res. Commun., 2000, 269, 633-640). Effectiveinhibition of ROCK 1 kinase activity has also led to conclusions thatinhibition of ROCK 1 may be an effective therapeutic target for severaldiseases including hypertension and bronchial asthma (Yoshii et al., Am.J. Respir. Cell Mol. Biol., 1999, 20, 1190-1200), glaucoma and otherocular diseases (Honjo et al., Invest. Ophthalmol. Vis. Sci., 2001, 42,137-144; Rao et al., Invest. Ophthalmol. Vis. Sci., 2001, 42,1029-1037), liver fibrogenesis (Iwamoto et al., J. Hepatol., 2000, 32,762-770; Tada et al., J. Hepatol., 2001, 34, 529-536), pulmonary edema(Chiba et al., Microvasc. Res., 2001, 62, 164-171), and intrahepaticmetastasis of hepatocellular carcinoma (Takamura et al., Hepatology,2001, 33, 577-581).

[0010] One use of an antisense oligonucleotide targeted to chicken ROCK1 has been reported in the art to study the role of ROCK 1 in vertebrateembryonic morphogenesis, and the results duplicated many of theteratologies induced by Y-27632 (Wei et al., Development, 2001, 128,2953-2962).

[0011] Consequently, there remains a long felt need for additionalagents capable of effectively inhibiting ROCK 1 function.

[0012] Antisense technology is emerging as an effective means forreducing the expression of specific gene products and may thereforeprove to be uniquely useful in a number of therapeutic, diagnostic, andresearch applications for the modulation of ROCK 1 expression.

[0013] The present invention provides compositions and methods formodulating ROCK 1 expression.

SUMMARY OF THE INVENTION

[0014] The present invention is directed to compounds, especiallynucleic acid and nucleic acid-like oligomers, which are targeted to anucleic acid encoding ROCK 1, and which modulate the expression ofROCK 1. Pharmaceutical and other compositions comprising the compoundsof the invention are also provided. Further provided are methods ofscreening for modulators of ROCK 1 and methods of modulating theexpression of ROCK 1 in cells, tissues or animals comprising contactingsaid cells, tissues or animals with one or more of the compounds orcompositions of the invention. Methods of treating an animal,particularly a human, suspected of having or being prone to a disease orcondition associated with expression of ROCK 1 are also set forthherein. Such methods comprise administering a therapeutically orprophylactically effective amount of one or more of the compounds orcompositions of the invention to the person in need of treatment.

DETAILED DESCRIPTION OF THE INVENTION A. Overview of the Invention

[0015] The present invention employs compounds, preferablyoligonucleotides and similar species for use in modulating the functionor effect of nucleic acid molecules encoding ROCK 1. This isaccomplished by providing oligonucleotides which specifically hybridizewith one or more nucleic acid molecules encoding ROCK 1. As used herein,the terms “target nucleic acid” and “nucleic acid molecule encoding ROCK1” have been used for convenience to encompass DNA encoding ROCK 1, RNA(including pre-mRNA and mRNA or portions thereof) transcribed from suchDNA, and also cDNA derived from such RNA. The hybridization of acompound of this invention with its target nucleic acid is generallyreferred to as “antisense”. Consequently, the preferred mechanismbelieved to be included in the practice of some preferred embodiments ofthe invention is referred to herein as “antisense inhibition.” Suchantisense inhibition is typically based upon hydrogen bonding-basedhybridization of oligonucleotide strands or segments such that at leastone strand or segment is cleaved, degraded, or otherwise renderedinoperable. In this regard, it is presently preferred to target specificnucleic acid molecules and their functions for such antisenseinhibition.

[0016] The functions of DNA to be interfered with can includereplication and transcription. Replication and transcription, forexample, can be from an endogenous cellular template, a vector, aplasmid construct or otherwise. The functions of RNA to be interferedwith can include functions such as translocation of the RNA to a site ofprotein translation, translocation of the RNA to sites within the cellwhich are distant from the site of RNA synthesis, translation of proteinfrom the RNA, splicing of the RNA to yield one or more RNA species, andcatalytic activity or complex formation involving the RNA which may beengaged in or facilitated by the RNA. One preferred result of suchinterference with target nucleic acid function is modulation of theexpression of ROCK 1. In the context of the present invention,“modulation” and “modulation of expression” mean either an increase(stimulation) or a decrease (inhibition) in the amount or levels of anucleic acid molecule encoding the gene, e.g., DNA or RNA. Inhibition isoften the preferred form of modulation of expression and mRNA is often apreferred target nucleic acid.

[0017] In the context of this invention, “hybridization” means thepairing of complementary strands of oligomeric compounds. In the presentinvention, the preferred mechanism of pairing involves hydrogen bonding,which may be Watson-Crick, Hoogsteen or reversed Hoogsteen hydrogenbonding, between complementary nucleoside or nucleotide bases(nucleobases) of the strands of oligomeric compounds. For example,adenine and thymine are complementary nucleobases which pair through theformation of hydrogen bonds. Hybridization can occur under varyingcircumstances.

[0018] An antisense compound is specifically hybridizable when bindingof the compound to the target nucleic acid interferes with the normalfunction of the target nucleic acid to cause a loss of activity, andthere is a sufficient degree of complementarity to avoid non-specificbinding of the antisense compound to non-target nucleic acid sequencesunder conditions in which specific binding is desired, i.e., underphysiological conditions in the case of in vivo assays or therapeutictreatment, and under conditions in which assays are performed in thecase of in vitro assays.

[0019] In the present invention the phrase “stringent hybridizationconditions” or “stringent conditions” refers to conditions under which acompound of the invention will hybridize to its target sequence, but toa minimal number of other sequences. Stringent conditions aresequence-dependent and will be different in different circumstances andin the context of this invention, “stringent conditions” under whicholigomeric compounds hybridize to a target sequence are determined bythe nature and composition of the oligomeric compounds and the assays inwhich they are being investigated.

[0020] “Complementary,” as used herein, refers to the capacity forprecise pairing between two nucleobases of an oligomeric compound. Forexample, if a nucleobase at a certain position of an oligonucleotide (anoligomeric compound), is capable of hydrogen bonding with a nucleobaseat a certain position of a target nucleic acid, said target nucleic acidbeing a DNA, RNA, or oligonucleotide molecule, then the position ofhydrogen bonding between the oligonucleotide and the target nucleic acidis considered to be a complementary position. The oligonucleotide andthe further DNA, RNA, or oligonucleotide molecule are complementary toeach other when a sufficient number of complementary positions in eachmolecule are occupied by nucleobases which can hydrogen bond with eachother. Thus, “specifically hybridizable” and “complementary” are termswhich are used to indicate a sufficient degree of precise pairing orcomplementarity over a sufficient number of nucleobases such that stableand specific binding occurs between the oligonucleotide and a targetnucleic acid.

[0021] It is understood in the art that the sequence of an antisensecompound need not be 100% complementary to that of its target nucleicacid to be specifically hybridizable. Moreover, an oligonucleotide mayhybridize over one or more segments such that intervening or adjacentsegments are not involved in the hybridization event (e.g., a loopstructure or hairpin structure). It is preferred that the antisensecompounds of the present invention comprise at least 70% sequencecomplementarity to a target region within the target nucleic acid, morepreferably that they comprise 90% sequence complementarity and even morepreferably comprise 95% sequence complementarity to the target regionwithin the target nucleic acid sequence to which they are targeted. Forexample, an antisense compound in which 18 of 20 nucleobases of theantisense compound are complementary to a target region, and wouldtherefore specifically hybridize, would represent 90 percentcomplementarity. In this example, the remaining noncomplementarynucleobases may be clustered or interspersed with complementarynucleobases and need not be contiguous to each other or to complementarynucleobases. As such, an antisense compound which is 18 nucleobases inlength having 4 (four) noncomplementary nucleobases which are flanked bytwo regions of complete complementarity with the target nucleic acidwould have 77.8% overall complementarity with the target nucleic acidand would thus fall within the scope of the present invention. Percentcomplementarity of an antisense compound with a region of a targetnucleic acid can be determined routinely using BLAST programs (basiclocal alignment search tools) and PowerBLAST programs known in the art(Altschul et al., J. Mol. Biol., 1990, 215, 403-410; Zhang and Madden,Genome Res., 1997, 7, 649-656).

B. Compounds of the Invention

[0022] According to the present invention, compounds include antisenseoligomeric compounds, antisense oligonucleotides, ribozymes, externalguide sequence (EGS) oligonucleotides, alternate splicers, primers,probes, and other oligomeric compounds which hybridize to at least aportion of the target nucleic acid. As such, these compounds may beintroduced in the form of single-stranded, double-stranded, circular orhairpin oligomeric compounds and may contain structural elements such asinternal or terminal bulges or loops. Once introduced to a system, thecompounds of the invention may elicit the action of one or more enzymesor structural proteins to effect modification of the target nucleicacid. One non-limiting example of such an enzyme is RNAse H, a cellularendonuclease which cleaves the RNA strand of an RNA:DNA duplex. It isknown in the art that single-stranded antisense compounds which are“DNA-like” elicit RNAse H. Activation of RNase H, therefore, results incleavage of the RNA target, thereby greatly enhancing the efficiency ofoligonucleotide-mediated inhibition of gene expression. Similar roleshave been postulated for other ribonucleases such as those in the RNaseIII and ribonuclease L family of enzymes.

[0023] While the preferred form of antisense compound is asingle-stranded antisense oligonucleotide, in many species theintroduction of double-stranded structures, such as double-stranded RNA(dsRNA) molecules, has been shown to induce potent and specificantisense-mediated reduction of the function of a gene or its associatedgene products. This phenomenon occurs in both plants and animals and isbelieved to have an evolutionary connection to viral defense andtransposon silencing.

[0024] The first evidence that dsRNA could lead to gene silencing inanimals came in 1995 from work in the nematode, Caenorhabditis elegans(Guo and Kempheus, Cell, 1995, 81, 611-620). Montgomery et al. haveshown that the primary interference effects of dsRNA areposttranscriptional (Montgomery et al., Proc. Natl. Acad. Sci. USA,1998, 95, 15502-15507). The posttranscriptional antisense mechanismdefined in Caenorhabditis elegans resulting from exposure todouble-stranded RNA (dsRNA) has since been designated RNA interference(RNAi). This term has been generalized to mean antisense-mediated genesilencing involving the introduction of dsRNA leading to thesequence-specific reduction of endogenous targeted mRNA levels (Fire etal., Nature, 1998, 391, 806-811). Recently, it has been shown that itis, in fact, the single-stranded RNA oligomers of antisense polarity ofthe dsRNAs which are the potent inducers of RNAi (Tijsterman et al.,Science, 2002, 295, 694-697).

[0025] In the context of this invention, the term “oligomeric compound”refers to a polymer or oligomer comprising a plurality of monomericunits. In the context of this invention, the term “oligonucleotide”refers to an oligomer or polymer of ribonucleic acid (RNA) ordeoxyribonucleic acid (DNA) or mimetics, chimeras, analogs and homologsthereof. This term includes oligonucleotides composed of naturallyoccurring nucleobases, sugars and covalent internucleoside (backbone)linkages as well as oligonucleotides having non-naturally occurringportions which function similarly. Such modified or substitutedoligonucleotides are often preferred over native forms because ofdesirable properties such as, for example, enhanced cellular uptake,enhanced affinity for a target nucleic acid and increased stability inthe presence of nucleases.

[0026] While oligonucleotides are a preferred form of the compounds ofthis invention, the present invention comprehends other families ofcompounds as well, including but not limited to oligonucleotide analogsand mimetics such as those described herein.

[0027] The compounds in accordance with this invention preferablycomprise from about 8 to about 80 nucleobases (i.e. from about 8 toabout 80 linked nucleosides). One of ordinary skill in the art willappreciate that the invention embodies compounds of 8, 9, 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30,31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48,49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, or 80 nucleobases inlength.

[0028] In one preferred embodiment, the compounds of the invention are12 to 50 nucleobases in length. One having ordinary skill in the artwill appreciate that this embodies compounds of 12, 13, 14, 15, 16, 17,18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35,36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50nucleobases in length.

[0029] In another preferred embodiment, the compounds of the inventionare 15 to 30 nucleobases in length. One having ordinary skill in the artwill appreciate that this embodies compounds of 15, 16, 17, 18, 19, 20,21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 nucleobases in length.

[0030] Particularly preferred compounds are oligonucleotides from about12 to about 50 nucleobases, even more preferably those comprising fromabout 15 to about 30 nucleobases.

[0031] Antisense compounds 8-80 nucleobases in length comprising astretch of at least eight (8) consecutive nucleobases selected fromwithin the illustrative antisense compounds are considered to besuitable antisense compounds as well.

[0032] Exemplary preferred antisense compounds include oligonucleotidesequences that comprise at least the 8 consecutive nucleobases from the5′-terminus of one of the illustrative preferred antisense compounds(the remaining nucleobases being a consecutive stretch of the sameoligonucleotide beginning immediately upstream of the 5′-terminus of theantisense compound which is specifically hybridizable to the targetnucleic acid and continuing until the oligonucleotide contains about 8to about 80 nucleobases). Similarly preferred antisense compounds arerepresented by oligonucleotide sequences that comprise at least the 8consecutive nucleobases from the 3′-terminus of one of the illustrativepreferred antisense compounds (the remaining nucleobases being aconsecutive stretch of the same oligonucleotide beginning immediatelydownstream of the 3′-terminus of the antisense compound which isspecifically hybridizable to the target nucleic acid and continuinguntil the oligonucleotide contains about 8 to about 80 nucleobases). Onehaving skill in the art armed with the preferred antisense compoundsillustrated herein will be able, without undue experimentation, toidentify further preferred antisense compounds.

C. Targets of the Invention

[0033] “Targeting” an antisense compound to a particular nucleic acidmolecule, in the context of this invention, can be a multistep process.The process usually begins with the identification of a target nucleicacid whose function is to be modulated. This target nucleic acid may be,for example, a cellular gene (or mRNA transcribed from the gene) whoseexpression is associated with a particular disorder or disease state, ora nucleic acid molecule from an infectious agent. In the presentinvention, the target nucleic acid encodes ROCK 1.

[0034] The targeting process usually also includes determination of atleast one target region, segment, or site within the target nucleic acidfor the antisense interaction to occur such that the desired effect,e.g., modulation of expression, will result. Within the context of thepresent invention, the term “region” is defined as a portion of thetarget nucleic acid having at least one identifiable structure,function, or characteristic. Within regions of target nucleic acids aresegments. “Segments” are defined as smaller or sub-portions of regionswithin a target nucleic acid. “Sites,” as used in the present invention,are defined as positions within a target nucleic acid.

[0035] Since, as is known in the art, the translation initiation codonis typically 5′-AUG (in transcribed mRNA molecules; 5′-ATG in thecorresponding DNA molecule), the translation initiation codon is alsoreferred to as the “AUG codon,” the “start codon” or the “AUG startcodon”. A minority of genes have a translation initiation codon havingthe RNA sequence 5′-GUG, 5′-UUG or 5′-CUG, and 5′-AUA, 5′-ACG and 5′-CUGhave been shown to function in vivo. Thus, the terms “translationinitiation codon” and “start codon” can encompass many codon sequences,even though the initiator amino acid in each instance is typicallymethionine (in eukaryotes) or formylmethionine (in prokaryotes). It isalso known in the art that eukaryotic and prokaryotic genes may have twoor more alternative start codons, any one of which may be preferentiallyutilized for translation initiation in a particular cell type or tissue,or under a particular set of conditions. In the context of theinvention, “start codon” and “translation initiation codon” refer to thecodon or codons that are used in vivo to initiate translation of an mRNAtranscribed from a gene encoding ROCK 1, regardless of the sequence(s)of such codons. It is also known in the art that a translationtermination codon (or “stop codon”) of a gene may have one of threesequences, i.e., 5′-UAA, 5′-UAG and 5′-UGA (the corresponding DNAsequences are 5′-TAA, 5′-TAG and 5′-TGA, respectively).

[0036] The terms “start codon region” and “translation initiation codonregion” refer to a portion of such an mRNA or gene that encompasses fromabout 25 to about 50 contiguous nucleotides in either direction (i.e.,5′ or 3′) from a translation initiation codon. Similarly, the terms“stop codon region” and “translation termination codon region” refer toa portion of such an mRNA or gene. that encompasses from about 25 toabout 50 contiguous nucleotides in either direction (i.e., 5′ or 3′)from a translation termination codon. Consequently, the “start codonregion” (or “translation initiation codon region”) and the “stop codonregion” (or “translation termination codon region”) are all regionswhich may be targeted effectively with the antisense compounds of thepresent invention.

[0037] The open reading frame (ORF) or “coding region,” which is knownin the art to refer to the region between the translation initiationcodon and the translation termination codon, is also a region which maybe targeted effectively. Within the context of the present invention, apreferred region is the intragenic region encompassing the translationinitiation or termination codon of the open reading frame (ORF) of agene.

[0038] Other target regions include the 5′ untranslated region (5′UTR),known in the art to refer to the portion of an mRNA in the 5′ directionfrom the translation initiation codon, and thus including nucleotidesbetween the 5′ cap site and the translation initiation codon of an mRNA(or corresponding nucleotides on the gene), and the 3′ untranslatedregion (3′UTR), known in the art to refer to the portion of an mRNA inthe 3′ direction from the translation termination codon, and thusincluding nucleotides between the translation termination codon and 3′end of an mRNA (or corresponding nucleotides on the gene). The 5′ capsite of an mRNA comprises an N7-methylated guanosine residue joined tothe 5′-most residue of the mRNA via a 5′-5′ triphosphate linkage. The 5′cap region of an mRNA is considered to include the 5′ cap structureitself as well as the first 50 nucleotides adjacent to the cap site. Itis also preferred to target the 5′ cap region.

[0039] Although some eukaryotic mRNA transcripts are directlytranslated, many contain one or more regions, known as “introns,” whichare excised from a transcript before it is translated. The remaining(and therefore translated) regions are known as “exons” and are splicedtogether to form a continuous mRNA sequence. Targeting splice sites,i.e., intron-exon junctions or exon-intron junctions, may also beparticularly useful in situations where aberrant splicing is implicatedin disease, or where an overproduction of a particular splice product isimplicated in disease. Aberrant fusion junctions due to rearrangementsor deletions are also preferred target sites. mRNA transcripts producedvia the process of splicing of two (or more) mRNAs from different genesources are known as “fusion transcripts”. It is also known that intronscan be effectively targeted using antisense compounds targeted to, forexample, DNA or pre-mRNA.

[0040] It is also known in the art that alternative RNA transcripts canbe produced from the same genomic region of DNA. These alternativetranscripts are generally known as “variants”. More specifically,“pre-mRNA variants” are transcripts produced from the same genomic DNAthat differ from other transcripts produced from the same genomic DNA ineither their start or stop position and contain both intronic and exonicsequence.

[0041] Upon excision of one or more exon or intron regions, or portionsthereof during splicing, pre-mRNA variants produce smaller “mRNAvariants”. Consequently, mRNA variants are processed pre-mRNA variantsand each unique pre-mRNA variant must always produce a unique mRNAvariant as a result of splicing. These mRNA variants are also known as“alternative splice variants”. If no splicing of the pre-mRNA variantoccurs then the pre-mRNA variant is identical to the mRNA variant.

[0042] It is also known in the art that variants can be produced throughthe use of alternative signals to start or stop transcription and thatpre-mRNAs and mRNAs can possess more that one start codon or stop codon.Variants that originate from a pre-mRNA or mRNA that use alternativestart codons are known as “alternative start variants” of that pre-mRNAor mRNA. Those transcripts that use an alternative stop codon are knownas “alternative stop variants” of that pre-mRNA or mRNA. One specifictype of alternative stop variant is the “polyA variant” in which themultiple transcripts produced result from the alternative selection ofone of the “polyA stop signals” by the transcription machinery, therebyproducing transcripts that terminate at unique polyA sites. Within thecontext of the invention, the types of variants described herein arealso preferred target nucleic acids.

[0043] The locations on the target nucleic acid to which the preferredantisense compounds hybridize are hereinbelow referred to as “preferredtarget segments.” As used herein the term “preferred target segment” isdefined as at least an 8-nucleobase portion of a target region to whichan active antisense compound is targeted. While not wishing to be boundby theory, it is presently believed that these target segments representportions of the target nucleic acid which are accessible forhybridization.

[0044] While the specific sequences of certain preferred target segmentsare set forth herein, one of skill in the art will recognize that theseserve to illustrate and describe particular embodiments within the scopeof the present invention. Additional preferred target segments may beidentified by one having ordinary skill.

[0045] Target segments 8-80 nucleobases in length comprising a stretchof at least eight (8) consecutive nucleobases selected from within theillustrative preferred target segments are considered to be suitable fortargeting as well.

[0046] Target segments can include DNA or RNA sequences that comprise atleast the 8 consecutive nucleobases from the 5′-terminus of one of theillustrative preferred target segments (the remaining nucleobases beinga consecutive stretch of the same DNA or RNA beginning immediatelyupstream of the 5′-terminus of the target segment and continuing untilthe DNA or RNA contains about 8 to about 80 nucleobases). Similarlypreferred target segments are represented by DNA or RNA sequences thatcomprise at least the 8 consecutive nucleobases from the 3′-terminus ofone of the illustrative preferred target segments (the remainingnucleobases being a consecutive stretch of the same DNA or RNA beginningimmediately downstream of the 3′-terminus of the target segment andcontinuing until the DNA or RNA contains about 8 to about 80nucleobases). One having skill in the art armed with the preferredtarget segments illustrated herein will be able, without undueexperimentation, to identify further preferred target segments.

[0047] Once one or more target regions, segments or sites have beenidentified, antisense compounds are chosen which are sufficientlycomplementary to the target, i.e., hybridize sufficiently well and withsufficient specificity, to give the desired effect.

D. Screening and Target Validation

[0048] In a further embodiment, the “preferred target segments”identified herein may be employed in a screen for additional compoundsthat modulate the expression of ROCK 1. “Modulators” are those compoundsthat decrease or increase the expression of a nucleic acid moleculeencoding ROCK 1 and which comprise at least an 8-nucleobase portionwhich is complementary to a preferred target segment. The screeningmethod comprises the steps of contacting a preferred target segment of anucleic acid molecule encoding ROCK 1 with one or more candidatemodulators, and selecting for one or more candidate modulators whichdecrease or increase the expression of a nucleic acid molecule encodingROCK 1. Once it is shown that the candidate modulator or modulators arecapable of modulating (e.g. either decreasing or increasing) theexpression of a nucleic acid molecule encoding ROCK 1, the modulator maythen be employed in further investigative studies of the function ofROCK 1, or for use as a research, diagnostic, or therapeutic agent inaccordance with the present invention.

[0049] The preferred target segments of the present invention may bealso be combined with their respective complementary antisense compoundsof the present invention to form stabilized double-stranded (duplexed)oligonucleotides.

[0050] Such double stranded oligonucleotide moieties have been shown inthe art to modulate target expression and regulate translation as wellas RNA processsing via an antisense mechanism. Moreover, thedouble-stranded moieties may be subject to chemical modifications (Fireet al., Nature, 1998, 391, 806-811; Timmons and Fire, Nature 1998, 395,854; Timmons et al., Gene, 2001, 263, 103-112; Tabara et al., Science,1998, 282, 430-431; Montgomery et al., Proc. Natl. Acad. Sci. USA, 1998,95, 15502-15507; Tuschl et al., Genes Dev., 1999, 13, 3191-3197;Elbashir et al., Nature, 2001, 411, 494-498; Elbashir et al., Genes Dev.2001, 15, 188-200). For example, such double-stranded moieties have beenshown to inhibit the target by the classical hybridization of antisensestrand of the duplex to the target, thereby triggering enzymaticdegradation of the target (Tijsterman et al., Science, 2002, 295,694-697).

[0051] The compounds of the present invention can also be applied in theareas of drug discovery and target validation. The present inventioncomprehends the use of the compounds and preferred target segmentsidentified herein in drug discovery efforts to elucidate relationshipsthat exist between ROCK 1 and a disease state, phenotype, or condition.These methods include detecting or modulating ROCK 1 comprisingcontacting a sample, tissue, cell, or organism with the compounds of thepresent invention, measuring the nucleic acid or protein level of ROCK 1and/or a related phenotypic or chemical endpoint at some time aftertreatment, and optionally comparing the measured value to a non-treatedsample or sample treated with a further compound of the invention. Thesemethods can also be performed in parallel or in combination with otherexperiments to determine the function of unknown genes for the processof target validation or to determine the validity of a particular geneproduct as a target for treatment or prevention of a particular disease,condition, or phenotype.

E. Kits, Research Reagents, Diagnostics, and Therapeutics

[0052] The compounds of the present invention can be utilized fordiagnostics, therapeutics, prophylaxis and as research reagents andkits. Furthermore, antisense oligonucleotides, which are able to inhibitgene expression with exquisite specificity, are often used by those ofordinary skill to elucidate the function of particular genes or todistinguish between functions of various members of a biologicalpathway.

[0053] For use in kits and diagnostics, the compounds of the presentinvention, either alone or in combination with other compounds ortherapeutics, can be used as tools in differential and/or combinatorialanalyses to elucidate expression patterns of a portion or the entirecomplement of genes expressed within cells and tissues.

[0054] As one nonlimiting example, expression patterns within cells ortissues treated with one or more antisense compounds are compared tocontrol cells or tissues not treated with antisense compounds and thepatterns produced are analyzed for differential levels of geneexpression as they pertain, for example, to disease association,signaling pathway, cellular localization, expression level, size,structure or function of the genes examined. These analyses can beperformed on stimulated or unstimulated cells and in the presence orabsence of other compounds which affect expression patterns.

[0055] Examples of methods of gene expression analysis known in the artinclude DNA arrays or microarrays (Brazma and Vilo, FEBS Lett., 2000,480, 17-24; Celis, et al., FEBS Lett., 2000, 480, 2-16), SAGE (serialanalysis of gene expression)(Madden, et al., Drug Discov. Today, 2000,5, 415-425), READS (restriction enzyme amplification of digested cDNAs)(Prashar and Weissman, Methods Enzymol., 1999, 303, 258-72), TOGA (totalgene expression analysis) (Sutcliffe, et al., Proc. Natl. Acad. Sci.U.S.A., 2000, 97, 1976-81), protein arrays and proteomics (Celis, etal., FEBS Lett., 2000, 480, 2-16; Jungblut, et al., Electrophoresis,1999, 20, 2100-10), expressed sequence tag (EST) sequencing (Celis, etal., FEBS Lett., 2000, 480, 2-16; Larsson, et al., J. Biotechnol., 2000,80, 143-57), subtractive RNA fingerprinting (SuRF) (Fuchs, et al., Anal.Biochem., 2000, 286, 91-98; Larson, et al., Cytometry, 2000, 41,203-208), subtractive cloning, differential display (DD) (Jurecic andBelmont, Curr. Opin. Microbiol., 2000, 3, 316-21), comparative genomichybridization (Carulli, et al., J. Cell Biochem. Suppl., 1998, 31,286-96), FISH (fluorescent in situ hybridization) techniques (Going andGusterson, Eur. J. Cancer, 1999, 35, 1895-904) and mass spectrometrymethods (To, Comb. Chem. High Throughput Screen, 2000, 3, 235-41).

[0056] The compounds of the invention are useful for research anddiagnostics, because these compounds hybridize to nucleic acids encodingROCK 1. For example, oligonucleotides that are shown to hybridize withsuch efficiency and under such conditions as disclosed herein as to beeffective ROCK 1 inhibitors will also be effective primers or probesunder conditions favoring gene amplification or detection, respectively.These primers and probes are useful in methods requiring the specificdetection of nucleic acid molecules encoding ROCK 1 and in theamplification of said nucleic acid molecules for detection or for use infurther studies of ROCK 1. Hybridization of the antisenseoligonucleotides, particularly the primers and probes, of the inventionwith a nucleic acid encoding ROCK 1 can be detected by means known inthe art. Such means may include conjugation of an enzyme to theoligonucleotide, radiolabelling of the oligonucleotide or any othersuitable detection means. Kits using such detection means for detectingthe level of ROCK 1 in a sample may also be prepared.

[0057] The specificity and sensitivity of antisense is also harnessed bythose of skill in the art for therapeutic uses. Antisense compounds havebeen employed as therapeutic moieties in the treatment of disease statesin animals, including humans. Antisense oligonucleotide drugs, includingribozymes, have been safely and effectively administered to humans andnumerous clinical trials are presently underway. It is thus establishedthat antisense compounds can be useful therapeutic modalities that canbe configured to be useful in treatment regimes for the treatment ofcells, tissues and animals, especially humans.

[0058] For therapeutics, an animal, preferably a human, suspected ofhaving a disease or disorder which can be treated by modulating theexpression of ROCK 1 is treated by administering antisense compounds inaccordance with this invention. For example, in one non-limitingembodiment, the methods comprise the step of administering to the animalin need of treatment, a therapeutically effective amount of a ROCK 1inhibitor. The ROCK 1 inhibitors of the present invention effectivelyinhibit the activity of the ROCK 1 protein or inhibit the expression ofthe ROCK 1 protein. In one embodiment, the activity or expression ofROCK 1 in an animal is inhibited by about 10%. Preferably, the activityor expression of ROCK 1 in an animal is inhibited by about 30%. Morepreferably, the activity or expression of ROCK 1 in an animal isinhibited by 50% or more.

[0059] For example, the reduction of the expression of ROCK 1 may bemeasured in serum, adipose tissue, liver or any other body fluid, tissueor organ of the animal. Preferably, the cells contained within saidfluids, tissues or organs being analyzed contain a nucleic acid moleculeencoding ROCK 1 protein and/or the ROCK 1 protein itself.

[0060] The compounds of the invention can be utilized in pharmaceuticalcompositions by adding an effective amount of a compound to a suitablepharmaceutically acceptable diluent or carrier. Use of the compounds andmethods of the invention may also be useful prophylactically.

F. Modifications

[0061] As is known in the art, a nucleoside is a base-sugar combination.The base portion of the nucleoside, is normally a heterocyclic base. Thetwo most common classes of such heterocyclic bases are the purines andthe pyrimidines. Nucleotides are nucleosides that further include aphosphate group covalently linked to the sugar portion of thenucleoside. For those nucleosides that include a pentofuranosyl sugar,the phosphate group can be linked to either the 2′, 3′ or 5′ hydroxylmoiety of the sugar. In forming oligonucleotides, the phosphate groupscovalently link adjacent nucleosides to one another to form a linearpolymeric compound. In turn, the respective ends of this linearpolymeric compound can be further joined to form a circular compound,however, linear compounds are generally preferred. In addition, linearcompounds may have internal nucleobase complementarity and may thereforefold in a manner as to produce a fully or partially double-strandedcompound. Within oligonucleotides, the phosphate groups are commonlyreferred to as forming the internucleoside backbone of theoligonucleotide. The normal linkage or backbone of RNA and DNA is a 3′to 5′ phosphodiester linkage.

[0062] Modified Internucleoside Linkages (Backbones)

[0063] Specific examples of preferred antisense compounds useful in thisinvention include oligonucleotides containing modified backbones ornon-natural internucleoside linkages. As defined in this specification,oligonucleotides having modified backbones include those that retain aphosphorus atom in the backbone and those that do not have a phosphorusatom in the backbone. For the purposes of this specification, and assometimes referenced in the art, modified oligonucleotides that do nothave a phosphorus atom in their internucleoside backbone can also beconsidered to be oligonucleosides.

[0064] Preferred modified oligonucleotide backbones containing aphosphorus atom therein include, for example, phosphorothioates, chiralphosphorothioates, phosphorodithioates, phosphotriesters,aminoalkylphosphotriesters, methyl and other alkyl phosphonatesincluding 3′-alkylene phosphonates, 5′-alkylene phosphonates and chiralphosphonates, phosphinates, phosphoramidates including 3′-aminophosphoramidate and aminoalkylphosphoramidates, thionophosphoramidates,thionoalkylphosphonates, thionoalkylphosphotriesters, selenophosphatesand boranophosphates having normal 3′-5′ linkages, 2′-5′ linked analogsof these, and those having inverted polarity wherein one or moreinternucleotide linkages is a 3′ to 3′, 5′ to 5′ or 2′ to 2′ linkage.Preferred oligonucleotides having inverted polarity comprise a single 3′to 3′ linkage at the 3′-most internucleotide linkage i.e. a singleinverted nucleoside residue which may be abasic (the nucleobase ismissing or has a hydroxyl group in place thereof). Various salts, mixedsalts and free acid forms are also included.

[0065] Representative United States patents that teach the preparationof the above phosphorus-containing linkages include, but are not limitedto, U.S. Pat. Nos.: 3,687,808; 4,469,863; 4,476,301; 5,023,243;5,177,196; 5,188,897; 5,264,423; 5,276,019; 5,278,302; 5,286,717;5,321,131; 5,399,676; 5,405,939; 5,453,496; 5,455,233; 5,466,677;5,476,925; 5,519,126; 5,536,821; 5,541,306; 5,550,111; 5,563,253;5,571,799; 5,587,361; 5,194,599; 5,565,555; 5,527,899; 5,721,218;5,672,697 and 5,625,050, certain of which are commonly owned with thisapplication, and each of which is herein incorporated by reference.

[0066] Preferred modified oligonucleotide backbones that do not includea phosphorus atom therein have backbones that are formed by short chainalkyl or cycloalkyl internucleoside linkages, mixed heteroatom and alkylor cycloalkyl internucleoside linkages, or one or more short chainheteroatomic or heterocyclic internucleoside linkages. These includethose having morpholino linkages (formed in part from the sugar portionof a nucleoside); siloxane backbones; sulfide, sulfoxide and sulfonebackbones; formacetyl and thioformacetyl backbones; methylene formacetyland thioformacetyl backbones; riboacetyl backbones; alkene containingbackbones; sulfamate backbones; methyleneimino and methylenehydrazinobackbones; sulfonate and sulfonamide backbones; amide backbones; andothers having mixed N, O, S and CH₂ component parts.

[0067] Representative United States patents that teach the preparationof the above oligonucleosides include, but are not limited to, U.S. Pat.Nos.: 5,034,506; 5,166,315; 5,185,444; 5,214,134; 5,216,141; 5,235,033;5,264,562; 5,264,564; 5,405,938; 5,434,257; 5,466,677; 5,470,967;5,489,677; 5,541,307; 5,561,225; 5,596,086; 5,602,240; 5,610,289;5,602,240; 5,608,046; 5,610,289; 5,618,704; 5,623,070; 5,663,312;5,633,360; 5,677,437; 5,792,608; 5,646,269 and 5,677,439, certain ofwhich are commonly owned with this application, and each of which isherein incorporated by reference.

[0068] Modified Sugar and Internucleoside Linkages-Mimetics

[0069] In other preferred oligonucleotide mimetics, both the sugar andthe internucleoside linkage (i.e. the backbone), of the nucleotide unitsare replaced with novel groups. The nucleobase units are maintained forhybridization with an appropriate target nucleic acid. One suchcompound, an oligonucleotide mimetic that has been shown to haveexcellent hybridization properties, is referred to as a peptide nucleicacid (PNA). In PNA compounds, the sugar-backbone of an oligonucleotideis replaced with an amide containing backbone, in particular anaminoethylglycine backbone. The nucleobases are retained and are bounddirectly or indirectly to aza nitrogen atoms of the amide portion of thebackbone. Representative United States patents that teach thepreparation of PNA compounds include, but are not limited to, U.S. Pat.Nos.: 5,539,082; 5,714,331; and 5,719,262, each of which is hereinincorporated by reference. Further teaching of PNA compounds can befound in Nielsen et al., Science, 1991, 254, 1497-1500.

[0070] Preferred embodiments of the invention are oligonucleotides withphosphorothioate backbones and oligonucleosides with heteroatombackbones, and in particular —CH₂—NH—O—CH₂—, —CH₂—N(CH₃)—O—CH₂— [knownas a methylene (methylimino) or MMI backbone], —CH₂—O—N(CH₃)—CH₂—,—CH₂—N(CH₃)—N(CH₃)—CH₂— and —O—N(CH₃)—CH₂—CH₂— [wherein the nativephosphodiester backbone is represented as —O—P—O—CH₂—] of the abovereferenced U.S. Pat. No. 5,489,677, and the amide backbones of the abovereferenced U.S. Pat. No. 5,602,240. Also preferred are oligonucleotideshaving morpholino backbone structures of the above-referenced U.S. Pat.No. 5,034,506.

[0071] Modified Sugars

[0072] Modified oligonucleotides may also contain one or moresubstituted sugar moieties. Preferred oligonucleotides comprise one ofthe following at the 2′ position: OH; F; O—, S—, or N-alkyl; O—, S—, orN-alkenyl; O—, S— or N-alkynyl; or O-alkyl-O-alkyl, wherein the alkyl,alkenyl and alkynyl may be substituted or unsubstituted C₁ to C₁₀ alkylor C₂ to C₁₀ alkenyl and alkynyl. Particularly preferred areO[(CH₂)_(n)O]_(m)CH₃, O(CH₂)_(n)OCH₃, O(CH₂)_(n)NH₂, O(CH₂)_(n)CH₃,O(CH₂)_(n)ONH₂, and O(CH₂)_(n)ON[(CH₂)_(n)CH₃]₂, where n and m are from1 to about 10. Other preferred oligonucleotides comprise one of thefollowing at the 2′ position: C₁ to C₁₀ lower alkyl, substituted loweralkyl, alkenyl, alkynyl, alkaryl, aralkyl, O-alkaryl or O-aralkyl, SH,SCH₃, OCN, Cl, Br, CN, CF₃, OCF₃, SOCH₃, SO₂CH₃, ONO₂, NO₂, N₃, NH₂,heterocycloalkyl, heterocycloalkaryl, aminoalkylamino, polyalkylamino,substituted silyl, an RNA cleaving group, a reporter group, anintercalator, a group for improving the pharmacokinetic properties of anoligonucleotide, or a group for improving the pharmacodynamic propertiesof an oligonucleotide, and other substituents having similar properties.A preferred modification includes 2′-methoxyethoxy (2′—O—CH₂CH₂OCH₃,also known as 2′-O-(2-methoxyethyl) or 2′-MOE) (Martin et al., Helv.Chim. Acta, 1995, 78, 486-504) i.e., an alkoxyalkoxy group. A furtherpreferred modification includes 2′-dimethylaminooxyethoxy, i.e., aO(CH₂)₂ON(CH₃)₂ group, also known as 2′-DMAOE, as described in exampleshereinbelow, and 2′-dimethylaminoethoxyethoxy (also known in the art as2′-O-dimethyl-amino-ethoxy-ethyl or 2′-DMAEOE), i.e.,2′-O—CH₂—O—CH₂—N(CH₃)₂, also described in examples hereinbelow.

[0073] Other preferred modifications include 2′-methoxy (2′-O—CH₃),2′-aminopropoxy (2′-OCH₂CH₂CH₂NH₂), 2′-allyl (2′-CH₂—CH═CH₂), 2′-O-allyl(2′-O—CH₂—CH═CH₂) and 2′-fluoro (2′-F). The 2′-modification may be inthe arabino (up) position or ribo (down) position. A preferred2′-arabino modification is 2′-F. Similar modifications may also be madeat other positions on the oligonucleotide, particularly the 3′ positionof the sugar on the 3′ terminal nucleotide or in 2′-5′ linkedoligonucleotides and the 5′ position of 5′ terminal nucleotide.Oligonucleotides may also have sugar mimetics such as cyclobutylmoieties in place of the pentofuranosyl sugar. Representative UnitedStates patents that teach the preparation of such modified sugarstructures include, but are not limited to, U.S. Pat. Nos.: 4,981,957;5,118,800; 5,319,080; 5,359,044; 5,393,878; 5,446,137; 5,466,786;5,514,785; 5,519,134; 5,567,811; 5,576,427; 5,591,722; 5,597,909;5,610,300; 5,627,053; 5,639,873; 5,646,265; 5,658,873; 5,670,633;5,792,747; and 5,700,920, certain of which are commonly owned with theinstant application, and each of which is herein incorporated byreference in its entirety.

[0074] A further preferred modification of the sugar includes LockedNucleic Acids (LNAs) in which the 2′-hydroxyl group is linked to the 3′or 4′ carbon atom of the sugar ring, thereby forming a bicyclic sugarmoiety. The linkage is preferably a methylene (—CH₂—)_(n) group bridgingthe 2′ oxygen atom and the 4′ carbon atom wherein n is 1 or 2. LNAs andpreparation thereof are described in WO 98/39352 and WO 99/14226.

[0075] Natural and Modified Nucleobases

[0076] Oligonucleotides may also include nucleobase (often referred toin the art simply as “base”) modifications or substitutions. As usedherein, “unmodified” or “natural” nucleobases include the purine basesadenine (A) and guanine (G), and the pyrimidine bases thymine (T),cytosine (C) and uracil (U). Modified nucleobases include othersynthetic and natural nucleobases such as 5-methylcytosine (5-me-C),5-hydroxymethyl cytosine, xanthine, hypoxanthine, 2-aminoadenine,6-methyl and other alkyl derivatives of adenine and guanine, 2-propyland other alkyl derivatives of adenine and guanine, 2-thiouracil,2-thiothymine and 2-thiocytosine, 5-halouracil and cytosine, 5-propynyl(—C≡C—CH₃) uracil and cytosine and other alkynyl derivatives ofpyrimidine bases, 6-azo uracil, cytosine and thymine, 5-uracil(pseudouracil), 4-thiouracil, 8-halo, 8-amino, 8-thiol, 8-thioalkyl,8-hydroxyl and other 8-substituted adenines and guanines, 5-haloparticularly 5-bromo, 5-trifluoromethyl and other 5-substituted uracilsand cytosines, 7-methylguanine and 7-methyladenine, 2-F-adenine,2-amino-adenine, 8-azaguanine and 8-azaadenine, 7-deazaguanine and7-deazaadenine and 3-deazaguanine and 3-deazaadenine. Further modifiednucleobases include tricyclic pyrimidines such as phenoxazinecytidine(1H-pyrimido[5,4-b][1,4]benzoxazin-2(3H)-one), phenothiazinecytidine (1H-pyrimido[5,4-b][1,4]benzothiazin-2(3H)-one), G-clamps suchas a substituted phenoxazine cytidine (e.g.9-(2-aminoethoxy)-H-pyrimido[5,4-b][1,4]benzoxazin-2(3H)-one), carbazolecytidine (2H-pyrimido[4,5-b]indol-2-one), pyridoindole cytidine(H-pyrido[3′,2′:4,5]pyrrolo[2,3-d]pyrimidin-2-one). Modified nucleobasesmay also include those in which the purine or pyrimidine base isreplaced with other heterocycles, for example 7-deaza-adenine,7-deazaguanosine, 2-aminopyridine and 2-pyridone. Further nucleobasesinclude those disclosed in U.S. Pat. No. 3,687,808, those disclosed inThe Concise Encyclopedia Of Polymer Science And Engineering, pages858-859, Kroschwitz, J. I., ed. John Wiley & Sons, 1990, those disclosedby Englisch et al., Angewandte Chemie, International Edition, 1991, 30,613, and those disclosed by Sanghvi, Y. S., Chapter 15, AntisenseResearch and Applications, pages 289-302, Crooke, S. T. and Lebleu, B.ed., CRC Press, 1993. Certain of these nucleobases are particularlyuseful for increasing the binding affinity of the compounds of theinvention. These include 5-substituted pyrimidines, 6-azapyrimidines andN-2, N-6 and O-6 substituted purines, including 2-aminopropyladenine,5-propynyluracil and 5-propynylcytosine. 5-methylcytosine substitutionshave been shown to increase nucleic acid duplex stability by 0.6-1.2° C.and are presently preferred base substitutions, even more particularlywhen combined with 2′-O-methoxyethyl sugar modifications.

[0077] Representative United States patents that teach the preparationof certain of the above noted modified nucleobases as well as othermodified nucleobases include, but are not limited to, the above notedU.S. Pat. No. 3,687,808, as well as U.S. Pat. Nos.: 4,845,205;5,130,302; 5,134,066; 5,175,273; 5,367,066; 5,432,272; 5,457,187;5,459,255; 5,484,908; 5,502,177; 5,525,711; 5,552,540; 5,587,469;5,594,121, 5,596,091; 5,614,617; 5,645,985; 5,830,653; 5,763,588;6,005,096; and 5,681,941, certain of which are commonly owned with theinstant application, and each of which is herein incorporated byreference, and U.S. Pat. No. 5,750,692, which is commonly owned with theinstant application and also herein incorporated by reference.

[0078] Conjugates

[0079] Another modification of the oligonucleotides of the inventioninvolves chemically linking to the oligonucleotide one or more moietiesor conjugates which enhance the activity, cellular distribution orcellular uptake of the oligonucleotide. These moieties or conjugates caninclude conjugate groups covalently bound to functional groups such asprimary or secondary hydroxyl groups. Conjugate groups of the inventioninclude intercalators, reporter molecules, polyamines, polyamides,polyethylene glycols, polyethers, groups that enhance thepharmacodynamic properties of oligomers, and groups that enhance thepharmacokinetic properties of oligomers. Typical conjugate groupsinclude cholesterols, lipids, phospholipids, biotin, phenazine, folate,phenanthridine, anthraquinone, acridine, fluoresceins, rhodamines,coumarins, and dyes. Groups that enhance the pharmacodynamic properties,in the context of this invention, include groups that improve uptake,enhance resistance to degradation, and/or strengthen sequence-specifichybridization with the target nucleic acid. Groups that enhance thepharmacokinetic properties, in the context of this invention, includegroups that improve uptake, distribution, metabolism or excretion of thecompounds of the present invention. Representative conjugate groups aredisclosed in International Patent Application PCT/US92/09196, filed Oct.23, 1992, and U.S. Pat. No. 6,287,860, the entire disclosure of whichare incorporated herein by reference. Conjugate moieties include but arenot limited to lipid moieties such as a cholesterol moiety, cholic acid,a thioether, e.g., hexyl-S-tritylthiol, a thiocholesterol, an aliphaticchain, e.g., dodecandiol or undecyl residues, a phospholipid, e.g.,di-hexadecyl-rac-glycerol or triethylammonium1,2-di-O-hexadecyl-rac-glycero-3-H-phosphonate, a polyamine or apolyethylene glycol chain, or adamantane acetic acid, a palmityl moiety,or an octadecylamine or hexylamino-carbonyl-oxycholesterol moiety.Oligonucleotides of the invention may also be conjugated to active drugsubstances, for example, aspirin, warfarin, phenylbutazone, ibuprofen,suprofen, fenbufen, ketoprofen, (S)-(+)-pranoprofen, carprofen,dansylsarcosine, 2,3,5-triiodobenzoic acid, flufenamic acid, folinicacid, a benzothiadiazide, chlorothiazide, a diazepine, indomethicin, abarbiturate, a cephalosporin, a sulfa drug, an antidiabetic, anantibacterial or an antibiotic. Oligonucleotide-drug conjugates andtheir preparation are described in U.S. patent application Ser. No.09/334,130 (filed Jun. 15, 1999) which is incorporated herein byreference in its entirety.

[0080] Representative United States patents that teach the preparationof such oligonucleotide conjugates include, but are not limited to, U.S.Pat. Nos.: 4,828,979; 4,948,882; 5,218,105; 5,525,465; 5,541,313;5,545,730; 5,552,538; 5,578,717, 5,580,731; 5,580,731; 5,591,584;5,109,124; 5,118,802; 5,138,045; 5,414,077; 5,486,603; 5,512,439;5,578,718; 5,608,046; 4,587,044; 4,605,735; 4,667,025; 4,762,779;4,789,737; 4,824,941; 4,835,263; 4,876,335; 4,904,582; 4,958,013;5,082,830; 5,112,963; 5,214,136; 5,082,830; 5,112,963; 5,214,136;5,245,022; 5,254,469; 5,258,506; 5,262,536; 5,272,250; 5,292,873;5,317,098; 5,371,241, 5,391,723; 5,416,203, 5,451,463; 5,510,475;5,512,667; 5,514,785; 5,565,552; 5,567,810; 5,574,142; 5,585,481;5,587,371; 5,595,726; 5,597,696; 5,599,923; 5,599,928 and 5,688,941,certain of which are commonly owned with the instant application, andeach of which is herein incorporated by reference.

[0081] Chimeric Compounds

[0082] It is not necessary for all positions in a given compound to beuniformly modified, and in fact more than one of the aforementionedmodifications may be incorporated in a single compound or even at asingle nucleoside within an oligonucleotide.

[0083] The present invention also includes antisense compounds which arechimeric compounds. “Chimeric” antisense compounds or “chimeras,” in thecontext of this invention, are antisense compounds, particularlyoligonucleotides, which contain two or more chemically distinct regions,each made up of at least one monomer unit, i.e., a nucleotide in thecase of an oligonucleotide compound. These oligonucleotides typicallycontain at least one region wherein the oligonucleotide is modified soas to confer upon the oligonucleotide increased resistance to nucleasedegradation, increased cellular uptake, increased stability and/orincreased binding affinity for the target nucleic acid. An additionalregion of the oligonucleotide may serve as a substrate for enzymescapable of cleaving RNA:DNA or RNA:RNA hybrids. By way of example, RNAseH is a cellular endonuclease which cleaves the RNA strand of an RNA:DNAduplex. Activation of RNase H, therefore, results in cleavage of the RNAtarget, thereby greatly enhancing the efficiency ofoligonucleotide-mediated inhibition of gene expression. The cleavage ofRNA:RNA hybrids can, in like fashion, be accomplished through theactions of endoribonucleases, such as RNAseL which cleaves both cellularand viral RNA. Cleavage of the RNA target can be routinely detected bygel electrophoresis and, if necessary, associated nucleic acidhybridization techniques known in the art.

[0084] Chimeric antisense compounds of the invention may be formed ascomposite structures of two or more oligonucleotides, modifiedoligonucleotides, oligonucleosides and/or oligonucleotide mimetics asdescribed above. Such compounds have also been referred to in the art ashybrids or gapmers. Representative United States patents that teach thepreparation of such hybrid structures include, but are not limited to,U.S. Pat. Nos.: 5,013,830; 5,149,797; 5,220,007; 5,256,775; 5,366,878;5,403,711; 5,491,133; 5,565,350; 5,623,065; 5,652,355; 5,652,356; and5,700,922, certain of which are commonly owned with the instantapplication, and each of which is herein incorporated by reference inits entirety.

G. Formulations

[0085] The compounds of the invention may also be admixed, encapsulated,conjugated or otherwise associated with other molecules, moleculestructures or mixtures of compounds, as for example, liposomes,receptor-targeted molecules, oral, rectal, topical or otherformulations, for assisting in uptake, distribution and/or absorption.Representative United States patents that teach the preparation of suchuptake, distribution and/or absorption-assisting formulations include,but are not limited to, U.S. Pat. Nos.: 5,108,921; 5,354,844; 5,416,016;5,459,127; 5,521,291; 5,543,158; 5,547,932; 5,583,020; 5,591,721;4,426,330; 4,534,899; 5,013,556; 5,108,921; 5,213,804; 5,227,170;5,264,221; 5,356,633; 5,395,619; 5,416,016; 5,417,978; 5,462,854;5,469,854; 5,512,295; 5,527,528; 5,534,259; 5,543,152; 5,556,948;5,580,575; and 5,595,756, each of which is herein incorporated byreference.

[0086] The antisense compounds of the invention encompass anypharmaceutically acceptable salts, esters, or salts of such esters, orany other compound which, upon administration to an animal, including ahuman, is capable of providing (directly or indirectly) the biologicallyactive metabolite or residue thereof. Accordingly, for example, thedisclosure is also drawn to prodrugs and pharmaceutically acceptablesalts of the compounds of the invention, pharmaceutically acceptablesalts of such prodrugs, and other bioequivalents.

[0087] The term “prodrug” indicates a therapeutic agent that is preparedin an inactive form that is converted to an active form (i.e., drug)within the body or cells thereof by the action of endogenous enzymes orother chemicals and/or conditions. In particular, prodrug versions ofthe oligonucleotides of the invention are prepared as SATE[(S-acetyl-2-thioethyl) phosphate] derivatives according to the methodsdisclosed in WO 93/24510 to Gosselin et al., published Dec. 9, 1993 orin WO 94/26764 and U.S. Pat. No. 5,770,713 to Imbach et al.

[0088] The term “pharmaceutically acceptable salts” refers tophysiologically and pharmaceutically acceptable salts of the compoundsof the invention: i.e., salts that retain the desired biologicalactivity of the parent compound and do not impart undesiredtoxicological effects thereto. For oligonucleotides, preferred examplesof pharmaceutically acceptable salts and their uses are furtherdescribed in U.S. Pat. No. 6,287,860, which is incorporated herein inits entirety.

[0089] The present invention also includes pharmaceutical compositionsand formulations which include the antisense compounds of the invention.The pharmaceutical compositions of the present invention may beadministered in a number of ways depending upon whether local orsystemic treatment is desired and upon the area to be treated.Administration may be topical (including ophthalmic and to mucousmembranes including vaginal and rectal delivery), pulmonary, e.g., byinhalation or insufflation of powders or aerosols, including bynebulizer; intratracheal, intranasal, epidermal and transdermal), oralor parenteral. Parenteral administration includes intravenous,intraarterial, subcutaneous, intraperitoneal or intramuscular injectionor infusion; or intracranial, e.g., intrathecal or intraventricular,administration. Oligonucleotides with at least one 2′-O-methoxyethylmodification are believed to be particularly useful for oraladministration. Pharmaceutical compositions and formulations for topicaladministration may include transdermal patches, ointments, lotions,creams, gels, drops, suppositories, sprays, liquids and powders.Conventional pharmaceutical carriers, aqueous, powder or oily bases,thickeners and the like may be necessary or desirable. Coated condoms,gloves and the like may also be useful.

[0090] The pharmaceutical formulations of the present invention, whichmay conveniently be presented in unit dosage form, may be preparedaccording to conventional techniques well known in the pharmaceuticalindustry. Such techniques include the step of bringing into associationthe active ingredients with the pharmaceutical carrier(s) orexcipient(s). In general, the formulations are prepared by uniformly andintimately bringing into association the active ingredients with liquidcarriers or finely divided solid carriers or both, and then, ifnecessary, shaping the product.

[0091] The compositions of the present invention may be formulated intoany of many possible dosage forms such as, but not limited to, tablets,capsules, gel capsules, liquid syrups, soft gels, suppositories, andenemas. The compositions of the present invention may also be formulatedas suspensions in aqueous, non-aqueous or mixed media. Aqueoussuspensions may further contain substances which increase the viscosityof the suspension including, for example, sodium carboxymethylcellulose,sorbitol and/or dextran. The suspension may also contain stabilizers.

[0092] Pharmaceutical compositions of the present invention include, butare not limited to, solutions, emulsions, foams and liposome-containingformulations. The pharmaceutical compositions and formulations of thepresent invention may comprise one or more penetration enhancers,carriers, excipients or other active or inactive ingredients.

[0093] Emulsions are typically heterogenous systems of one liquiddispersed in another in the form of droplets usually exceeding 0.1 μm indiameter. Emulsions may contain additional components in addition to thedispersed phases, and the active drug which may be present as a solutionin either the aqueous phase, oily phase or itself as a separate phase.Microemulsions are included as an embodiment of the present invention.Emulsions and their uses are well known in the art and are furtherdescribed in U.S. Pat. No. 6,287,860, which is incorporated herein inits entirety.

[0094] Formulations of the present invention include liposomalformulations. As used in the present invention, the term “liposome”means a vesicle composed of amphiphilic lipids arranged in a sphericalbilayer or bilayers. Liposomes are unilamellar or multilamellar vesicleswhich have a membrane formed from a lipophilic material and an aqueousinterior that contains the composition to be delivered. Cationicliposomes are positively charged liposomes which are believed tointeract with negatively charged DNA molecules to form a stable complex.Liposomes that are pH-sensitive or negatively-charged are believed toentrap DNA rather than complex with it. Both cationic and noncationicliposomes have been used to deliver DNA to cells.

[0095] Liposomes also include “sterically stabilized” liposomes, a termwhich, as used herein, refers to liposomes comprising one or morespecialized lipids that, when incorporated into liposomes, result inenhanced circulation lifetimes relative to liposomes lacking suchspecialized lipids. Examples of sterically stabilized liposomes arethose in which part of the vesicle-forming lipid portion of the liposomecomprises one or more glycolipids or is derivatized with one or morehydrophilic polymers, such as a polyethylene glycol (PEG) moiety.Liposomes and their uses are further described in U.S. Pat. No.6,287,860, which is incorporated herein in its entirety.

[0096] The pharmaceutical formulations and compositions of the presentinvention may also include surfactants. The use of surfactants in drugproducts, formulations and in emulsions is well known in the art.Surfactants and their uses are further described in U.S. Pat. No.6,287,860, which is incorporated herein in its entirety.

[0097] In one embodiment, the present invention employs variouspenetration enhancers to effect the efficient delivery of nucleic acids,particularly oligonucleotides. In addition to aiding the diffusion ofnon-lipophilic drugs across cell membranes, penetration enhancers alsoenhance the permeability of lipophilic drugs. Penetration enhancers maybe classified as belonging to one of five broad categories, i.e.,surfactants, fatty acids, bile salts, chelating agents, andnon-chelating non-surfactants. Penetration enhancers and their uses arefurther described in U.S. Pat. No. 6,287,860, which is incorporatedherein in its entirety.

[0098] One of skill in the art will recognize that formulations areroutinely designed according to their intended use, i.e. route ofadministration.

[0099] Preferred formulations for topical administration include thosein which the oligonucleotides of the invention are in admixture with atopical delivery agent such as lipids, liposomes, fatty acids, fattyacid esters, steroids, chelating agents and surfactants. Preferredlipids and liposomes include neutral (e.g. dioleoylphosphatidyl DOPEethanolamine, dimyristoylphosphatidyl choline DMPC,distearolyphosphatidyl choline) negative (e.g. dimyristoylphosphatidylglycerol DMPG) and cationic (e.g. dioleoyltetramethylaminopropyl DOTAPand dioleoylphosphatidyl ethanolamine DOTMA).

[0100] For topical or other administration, oligonucleotides of theinvention may be encapsulated within liposomes or may form complexesthereto, in particular to cationic liposomes. Alternatively,oligonucleotides may be complexed to lipids, in particular to cationiclipids. Preferred fatty acids and esters, pharmaceutically acceptablesalts thereof, and their uses are further described in U.S. Pat. No.6,287,860, which is incorporated herein in its entirety. Topicalformulations are described in detail in U.S. patent application Ser. No.09/315,298 filed on May 20, 1999, which is incorporated herein byreference in its entirety.

[0101] Compositions and formulations for oral administration includepowders or granules, microparticulates, nanoparticulates, suspensions orsolutions in water or non-aqueous media, capsules, gel capsules,sachets, tablets or minitablets. Thickeners, flavoring agents, diluents,emulsifiers, dispersing aids or binders may be desirable. Preferred oralformulations are those in which oligonucleotides of the invention areadministered in conjunction with one or more penetration enhancerssurfactants and chelators. Preferred surfactants include fatty acidsand/or esters or salts thereof, bile acids and/or salts thereof.Preferred bile acids/salts and fatty acids and their uses are furtherdescribed in U.S. Pat. No. 6,287,860, which is incorporated herein inits entirety. Also preferred are combinations of penetration enhancers,for example, fatty acids/salts in combination with bile acids/salts. Aparticularly preferred combination is the sodium salt of lauric acid,capric acid and UDCA. Further penetration enhancers includepolyoxyethylene-9-lauryl ether, polyoxyethylene-20-cetyl ether.Oligonucleotides of the invention may be delivered orally, in granularform including sprayed dried particles, or complexed to form micro ornanoparticles. Oligonucleotide complexing agents and their uses arefurther described in U.S. Pat. No. 6,287,860, which is incorporatedherein in its entirety. Oral formulations for oligonucleotides and theirpreparation are described in detail in U.S. application Ser. Nlo.09/108,673 (filed Jul. 1, 1998), Ser. No. 09/315,298 (filed May 20,1999) and Ser. No. 10/071,822, filed Feb. 8, 2002, each of which isincorporated herein by reference in their entirety.

[0102] Compositions and formulations for parenteral, intrathecal orintraventricular administration may include sterile aqueous solutionswhich may also contain buffers, diluents and other suitable additivessuch as, but not limited to, penetration enhancers, carrier compoundsand other pharmaceutically acceptable carriers or excipients.

[0103] Certain embodiments of the invention provide pharmaceuticalcompositions containing one or more oligomeric compounds and one or moreother chemotherapeutic agents which function by a non-antisensemechanism. Examples of such chemotherapeutic agents include but are notlimited to cancer chemotherapeutic drugs such as daunorubicin,daunomycin, dactinomycin, doxorubicin, epirubicin, idarubicin,esorubicin, bleomycin, mafosfamide, ifosfamide, cytosine arabinoside,bis-chloroethylnitrosurea, busulfan, mitomycin C, actinomycin D,mithramycin, prednisone, hydroxyprogesterone, testosterone, tamoxifen,dacarbazine, procarbazine, hexamethylmelamine, pentamethylmelamine,mitoxantrone, amsacrine, chlorambucil, methylcyclohexylnitrosurea,nitrogen mustards, melphalan, cyclophosphamide, 6-mercaptopurine,6-thioguanine, cytarabine, 5-azacytidine, hydroxyurea, deoxycoformycin,4-hydroxyperoxycyclophosphoramide, 5-fluorouracil (5-FU),5-fluorodeoxyuridine (5-FUdR), methotrexate (MTX), colchicine, taxol,vincristine, vinblastine, etoposide (VP-16), trimetrexate, irinotecan,topotecan, gemcitabine, teniposide, cisplatin and diethylstilbestrol(DES). When used with the compounds of the invention, suchchemotherapeutic agents may be used individually (e.g., 5-FU andoligonucleotide), sequentially (e.g., 5-FU and oligonucleotide for aperiod of time followed by MTX and oligonucleotide), or in combinationwith one or more other such chemotherapeutic agents (e.g., 5-FU, MTX andoligonucleotide, or 5-FU, radiotherapy and oligonucleotide).Anti-inflammatory drugs, including but not limited to nonsteroidalanti-inflammatory drugs and corticosteroids, and antiviral drugs,including but not limited to ribivirin, vidarabine, acyclovir andganciclovir, may also be combined in compositions of the invention.Combinations of antisense compounds and other non-antisense drugs arealso within the scope of this invention. Two or more combined compoundsmay be used together or sequentially.

[0104] In another related embodiment, compositions of the invention maycontain one or more antisense compounds, particularly oligonucleotides,targeted to a first nucleic acid and one or more additional antisensecompounds targeted to a second nucleic acid target. Alternatively,compositions of the invention may contain two or more antisensecompounds targeted to different regions of the same nucleic acid target.Numerous examples of antisense compounds are known in the art. Two ormore combined compounds may be used together or sequentially.

H. Dosing

[0105] The formulation of therapeutic compositions and their subsequentadministration (dosing) is believed to be within the skill of those inthe art. Dosing is dependent on severity and responsiveness of thedisease state to be treated, with the course of treatment lasting fromseveral days to several months, or until a cure is effected or adiminution of the disease state is achieved. Optimal dosing schedulescan be calculated from measurements of drug accumulation in the body ofthe patient. Persons of ordinary skill can easily determine optimumdosages, dosing methodologies and repetition rates. Optimum dosages mayvary depending on the relative potency of individual oligonucleotides,and can generally be estimated based on EC₅₀s found to be effective inin vitro and in vivo animal models. In general, dosage is from 0.01 ugto 100 g per kg of body weight, and may be given once or more daily,weekly, monthly or yearly, or even once every 2 to 20 years. Persons ofordinary skill in the art can easily estimate repetition rates fordosing based on measured residence times and concentrations of the drugin bodily fluids or tissues. Following successful treatment, it may bedesirable to have the patient undergo maintenance therapy to prevent therecurrence of the disease state, wherein the oligonucleotide isadministered in maintenance doses, ranging from 0.01 ug to 100 g per kgof body weight, once or more daily, to once every 20 years.

[0106] While the present invention has been described with specificityin accordance with certain of its preferred embodiments, the followingexamples serve only to illustrate the invention and are not intended tolimit the same.

EXAMPLES Example 1

[0107] Synthesis of Nucleoside Phosphoramidites

[0108] The following compounds, including amidites and theirintermediates were prepared as described in U.S. Pat. No. 6,426,220 andpublished PCT WO 02/36743; 5′-O-Dimethoxytrityl-thymidine intermediatefor 5-methyl dC amidite, 5′-O-Dimethoxytrityl-2′-deoxy-5-methylcytidineintermediate for 5-methyl-dC amidite,5′-O-Dimethoxytrityl-2′-deoxy—N4-benzoyl-5-methylcytidine penultimateintermediate for 5-methyl dC amidite,[5′-O-(4,4′-Dimethoxytriphenylmethyl)-2′-deoxy-N⁴-benzoyl-5-methylcytidin-3′-O-yl]-2-cyanoethyl-N,N-diisopropylphosphoramidite(5-methyl dC amidite), 2′-Fluorodeoxyadenosine, 2′-Fluorodeoxyguanosine,2′-Fluorouridine, 2′-Fluorodeoxycytidine, 2′-O-(2-Methoxyethyl) modifiedamidites, 2′-O-(2-methoxyethyl)-5-methyluridine intermediate,5′-O-DMT-2′-O-(2-methoxyethyl)-5-methyluridine penultimate intermediate,[5′-O-(4,4′-Dimethoxytriphenylmethyl)-2′-O-(2-methoxyethyl)-5-methyluridin-3′-O-yl]-2-cyanoethyl-N,N-diisopropylphosphoramidite(MOE T amidite),5′-O-Dimethoxytrityl-2′-O-(2-methoxyethyl)-5-methylcytidineintermediate,5′-O-dimethoxytrityl-2′-O-(2-methoxyethyl)-N⁴-benzoyl-5-methyl-cytidinepenultimate intermediate,[5′-O-(4,4′-Dimethoxytriphenylmethyl)-2′-O-(2-methoxyethyl)-N⁴-benzoyl-5-methylcytidin-3′-O-yl]-2-cyanoethyl-N,N-diisopropylphosphoramidite(MOE 5-Me-C amidite),[5′-O-(4,4′-Dimethoxytriphenylmethyl)-2′-O-(2-methoxyethyl)-N⁴-benzoyladenosin-3′-O-yl]-2-cyanoethyl-N,N-diisopropylphosphoramidite(MOE A amdite),[5′-O-(4,4′-Dimethoxytriphenylmethyl)-2′-O-(2-methoxyethyl)-N⁴-isobutyrylguanosin-3′-O-yl]-2-cyanoethyl-N,N-diisopropylphosphoramidite(MOE G amidite), 2′-O-(Aminooxyethyl) nucleoside amidites and2′-O-(dimethylamino-oxyethyl) nucleoside amidites,2′-(Dimethylaminooxyethoxy) nucleoside amidites,5′-O-tert-Butyldiphenylsilyl-O²-2′-anhydro-5-methyluridine ,5′-O-tert-Butyldiphenylsilyl-2′-O-(2-hydroxyethyl)-5-methyluridine,2′-O-([2-phthalimidoxy)ethyl]-5′-t-butyldiphenylsilyl-5-methyluridine5′-O-tert-butyldiphenylsilyl-2′-O-[(2-formadoximinooxy)ethyl]-5-methyluridine,5′-O-tert-Butyldiphenylsilyl-2′-O-[N,N-dimethylaminooxyethyl]-5-methyluridine,2′-O-(dimethylaminooxyethyl)-5-methyluridine,5′-O-DMT-2′-O-(dimethylaminooxyethyl)-5-methyluridine,5′-O-DMT-2′-O-(2-N,N-dimethylaminooxyethyl)-5-methyluridine-3′-[(2-cyanoethyl)-N,N-diisopropylphosphoramidite],2′-(Aminooxyethoxy) nucleoside amidites,N2-isobutyryl-6-O-diphenylcarbamoyl-2′-O-(2-ethylacetyl)-5′-O-(4,4′-dimethoxytrityl)guanosine-3′-[(2-cyanoethyl)-N,N-diisopropylphosphoramidite],2′-dimethylaminoethoxyethoxy (2′-DMAEOE) nucleoside amidites,2′-O-[2(2-N,N-dimethylaminoethoxy)ethyl]-5-methyl uridine,5′-O-dimethoxytrityl-2′-O-[2(2-N,N-dimethylaminoethoxy)-ethyl)]-5-methyluridine and5′-O-Dimethoxytrityl-2′-O-[2(2-N,N-dimethylaminoethoxy)-ethyl)]-5-methyluridine-3′-O-(cyanoethyl—N,N-diisopropyl)phosphoramidite.

Example 2

[0109] Oligonucleotide and Oligonucleoside Synthesis

[0110] The antisense compounds used in accordance with this inventionmay be conveniently and routinely made through the well-known techniqueof solid phase synthesis. Equipment for such synthesis is sold byseveral vendors including, for example, Applied Biosystems (Foster City,Calif.). Any other means for such synthesis known in the art mayadditionally or alternatively be employed. It is well known to usesimilar techniques to prepare oligonucleotides such as thephosphorothioates and alkylated derivatives.

[0111] Oligonucleotides: Unsubstituted and substituted phosphodiester(P═O) oligonucleotides are synthesized on an automated DNA synthesizer(Applied Biosystems model 394) using standard phosphoramidite chemistrywith oxidation by iodine.

[0112] Phosphorothioates (P═S) are synthesized similar to phosphodiesteroligonucleotides with the following exceptions: thiation was effected byutilizing a 10% w/v solution of 3,H-1,2-benzodithiole-3-one 1,1-dioxidein acetonitrile for the oxidation of the phosphite linkages. Thethiation reaction step time was increased to 180 sec and preceded by thenormal capping step. After cleavage from the CPG column and deblockingin concentrated ammonium hydroxide at 55° C. (12-16 hr), theoligonucleotides were recovered by precipitating with >3 volumes ofethanol from a 1 M NH₄OAc solution. Phosphinate oligonucleotides areprepared as described in U.S. Pat. No. 5,508,270, herein incorporated byreference.

[0113] Alkyl phosphonate oligonucleotides are prepared as described inU.S. Pat. No. 4,469,863, herein incorporated by reference.

[0114] 3′-Deoxy-3′-methylene phosphonate oligonucleotides are preparedas described in U.S. Pat. Nos. 5,610,289 or 5,625,050, hereinincorporated by reference.

[0115] Phosphoramidite oligonucleotides are prepared as described inU.S. Pat. No., 5,256,775 or U.S. Pat. No. 5,366,878, herein incorporatedby reference.

[0116] Alkylphosphonothioate oligonucleotides are prepared as describedin published PCT applications PCT/US94/00902 and PCT/US93/06976(published as WO 94/17093 and WO 94/02499, respectively), hereinincorporated by reference.

[0117] 3′-Deoxy-3′-amino phosphoramidate oligonucleotides are preparedas described in U.S. Pat. No. 5,476,925, herein incorporated byreference.

[0118] Phosphotriester oligonucleotides are prepared as described inU.S. Pat. No. 5,023,243, herein incorporated by reference.

[0119] Borano phosphate oligonucleotides are prepared as described inU.S. Pat. Nos. 5,130,302 and 5,177,198, both herein incorporated byreference.

[0120] Oligonucleosides: Methylenemethylimino linked oligonucleosides,also identified as MMI linked oligonucleosides, methylenedimethylhydrazolinked oligonucleosides, also identified as MDH linked oligonucleosides,and methylenecarbonylamino linked oligonucleosides, also identified asamide-3 linked oligonucleosides, and methyleneaminocarbonyl linkedoligonucleosides, also identified as amide-4 linked oligonucleosides, aswell as mixed backbone compounds having, for instance, alternating MMIand P═O or P═S linkages are prepared as described in U.S. Pat. Nos.5,378,825, 5,386,023, 5,489,677, 5,602,240 and 5,610,289, all of whichare herein incorporated by reference.

[0121] Formacetal and thioformacetal linked oligonucleosides areprepared as described in U.S. Pat. Nos. 5,264,562 and 5,264,564, hereinincorporated by reference.

[0122] Ethylene oxide linked oligonucleosides are prepared as describedin U.S. Pat. No. 5,223,618, herein incorporated by reference.

Example 3

[0123] RNA Synthesis

[0124] In general, RNA synthesis chemistry is based on the selectiveincorporation of various protecting groups at strategic intermediaryreactions. Although one of ordinary skill in the art will understand theuse of protecting groups in organic synthesis, a useful class ofprotecting groups includes silyl ethers. In particular bulky silylethers are used to protect the 5′-hydroxyl in combination with anacid-labile orthoester protecting group on the 2′-hydroxyl. This set ofprotecting groups is then used with standard solid-phase synthesistechnology. It is important to lastly remove the acid labile orthoesterprotecting group after all other synthetic steps. Moreover, the earlyuse of the silyl protecting groups during synthesis ensures facileremoval when desired, without undesired deprotection of 2′ hydroxyl.

[0125] Following this procedure for the sequential protection of the5′-hydroxyl in combination with protection of the 2′-hydroxyl byprotecting groups that are differentially removed and are differentiallychemically labile, RNA oligonucleotides were synthesized.

[0126] RNA oligonucleotides are synthesized in a stepwise fashion. Eachnucleotide is added sequentially (3′- to 5′-direction) to a solidsupport-bound oligonucleotide. The first nucleoside at the 3′-end of thechain is covalently attached to a solid support. The nucleotideprecursor, a ribonucleoside phosphoramidite, and activator are added,coupling the second base onto the 5′-end of the first nucleoside. Thesupport is washed and any unreacted 5′-hydroxyl groups are capped withacetic anhydride to yield 5′-acetyl moieties. The linkage is thenoxidized to the more stable and ultimately desired P(V) linkage. At theend of the nucleotide addition cycle, the 5′-silyl group is cleaved withfluoride. The cycle is repeated for each subsequent nucleotide.

[0127] Following synthesis, the methyl protecting groups on thephosphates are cleaved in 30 minutes utilizing 1 Mdisodium-2-carbamoyl-2-cyanoethylene-1,1-dithiolate trihydrate (S₂Na₂)in DMF. The deprotection solution is washed from the solid support-boundoligonucleotide using water. The support is then treated with 40%methylamine in water for 10 minutes at 55° C. This releases the RNAoligonucleotides into solution, deprotects the exocyclic amines, andmodifies the 2′-groups. The oligonucleotides can be analyzed by anionexchange HPLC at this stage.

[0128] The 2′-orthoester groups are the last protecting groups to beremoved. The ethylene glycol monoacetate orthoester protecting groupdeveloped by Dharmacon Research, Inc. (Lafayette, CO), is one example ofa useful orthoester protecting group which, has the following importantproperties. It is stable to the conditions of nucleoside phosphoramiditesynthesis and oligonucleotide synthesis. However, after oligonucleotidesynthesis the oligonucleotide is treated with methylamine which not onlycleaves the oligonucleotide from the solid support but also removes theacetyl groups from the orthoesters. The resulting 2-ethylhydroxylsubstituents on the orthoester are less electron withdrawing than theacetylated precursor. As a result, the modified orthoester becomes morelabile to acid-catalyzed hydrolysis. Specifically, the rate of cleavageis approximately 10 times faster after the acetyl groups are removed.Therefore, this orthoester possesses sufficient stability in order to becompatible with oligonucleotide synthesis and yet, when subsequentlymodified, permits deprotection to be carried out under relatively mildaqueous conditions compatible with the final RNA oligonucleotideproduct.

[0129] Additionally, methods of RNA synthesis are well known in the art(Scaringe, S. A. Ph.D. Thesis, University of Colorado, 1996; Scaringe,S. A., et al., J. Am. Chem. Soc., 1998, 120, 11820-11821; Matteucci, M.D. and Caruthers, M. H. J. Am. Chem. Soc., 1981, 103, 3185-3191;Beaucage, S. L. and Caruthers, M. H. Tetrahedron Lett., 1981, 22,1859-1862; Dahl, B. J., et al., Acta Chem. Scand,. 1990, 44, 639-641;Reddy, M. P., et al., Tetrahedron Lett., 1994, 25, 4311-4314; Wincott,F. et al., Nucleic Acids Res., 1995, 23, 2677-2684; Griffin, B. E., etal., Tetrahedron, 1967, 23, 2301-2313; Griffin, B. E., et al.,Tetrahedron, 1967, 23, 2315-2331).

[0130] RNA antisense compounds (RNA oligonucleotides) of the presentinvention can be synthesized by the methods herein or purchased fromDharmacon Research, Inc (Lafayette, Colo.). Once synthesized,complementary RNA antisense compounds can then be annealed by methodsknown in the art to form double stranded (duplexed) antisense compounds.For example, duplexes can be formed by combining 30 μl of each of thecomplementary strands of RNA oligonucleotides (50 uM RNA oligonucleotidesolution) and 15 μl of 5× annealing buffer (100 mM potassium acetate, 30mM HEPES-KOH pH 7.4, 2 mM magnesium acetate) followed by heating for 1minute at 90° C., then 1 hour at 37° C. The resulting duplexed antisensecompounds can be used in kits, assays, screens, or other methods toinvestigate the role of a target nucleic acid.

Example 4

[0131] Synthesis of Chimeric Oligonucleotides

[0132] Chimeric oligonucleotides, oligonucleosides or mixedoligonucleotides/oligonucleosides of the invention can be of severaldifferent types. These include a first type wherein the “gap” segment oflinked nucleosides is positioned between 5′ and 3′ “wing” segments oflinked nucleosides and a second “open end” type wherein the “gap”segment is located at either the 3′ or the 5′ terminus of the oligomericcompound. Oligonucleotides of the first type are also known in the artas “gapmers” or gapped oligonucleotides. Oligonucleotides of the secondtype are also known in the art as “hemimers” or “wingmers”.

[0133] [2′-O-Me]-[2′-deoxy]-[2′-O-Me] Chimeric PhosphorothioateOligonucleotides

[0134] Chimeric oligonucleotides having 2′-O-alkyl phosphorothioate and2′-deoxy phosphorothioate oligonucleotide segments are synthesized usingan Applied Biosystems automated DNA synthesizer Model 394, as above.Oligonucleotides are synthesized using the automated synthesizer and 2′-deoxy-5′-dimethoxytrityl-3′-O-phosphoramidite for the DNA portion and5′-dimethoxytrityl-2′-O-methyl-3′-O-phosphoramidite for 5′ and 3′ wings.The standard synthesis cycle is modified by incorporating coupling stepswith increased reaction times for the5′-dimethoxytrityl-2′-O-methyl-3′-O-phosphoramidite. The fully protectedoligonucleotide is cleaved from the support and deprotected inconcentrated ammonia (NH₄OH) for 12-16 hr at 55° C. The deprotectedoligo is then recovered by an appropriate method (precipitation, columnchromatography, volume reduced in vacuo and analyzedspetrophotometrically for yield and for purity by capillaryelectrophoresis and by mass spectrometry.

[0135] [2′-O-(2-Methoxyethyl)]-[2′-deoxy]-[2′-O-(Methoxyethyl)] ChimericPhosphorothioate Oligonucleotides

[0136] [2′-O-(2-methoxyethyl)]-[2′-deoxy]-[-2′-O-(methoxyethyl)]chimeric phosphorothioate oligonucleotides were prepared as per theprocedure above for the 2′-O-methyl chimeric oligonucleotide, with thesubstitution of 2′-O-(methoxyethyl) amidites for the 2′-O-methylamidites.

[0137][2′-O-(2-Methoxyethyl)Phosphodiester]-[2′-deoxyPhosphorothioate]-[2-O-(2-Methoxyethyl)Phosphodiester] Chimeric Oligonucleotides

[0138] [2′-O-(2-methoxyethylphosphodiester]-[2′-deoxyphosphorothioate]-[2′-O-(methoxyethyl)phosphodiester] chimeric oligonucleotides are prepared as per the aboveprocedure for the 2′-O-methyl chimeric oligonucleotide with thesubstitution of 2′-O-(methoxyethyl) amidites for the 2′-O-methylamidites, oxidation with iodine to generate the phosphodiesterinternucleotide linkages within the wing portions of the chimericstructures and sulfurization utilizing 3,H-1,2 benzodithiole-3-one 1,1dioxide (Beaucage Reagent) to generate the phosphorothioateinternucleotide linkages for the center gap.

[0139] Other chimeric oligonucleotides, chimeric oligonucleosides andmixed chimeric oligonucleotides/oligonucleosides are synthesizedaccording to U.S. Pat. No. 5,623,065, herein incorporated by reference.

Example 5

[0140] Design and Screening of Duplexed Antisense Compounds TargetingROCK 1

[0141] In accordance with the present invention, a series of nucleicacid duplexes comprising the antisense compounds of the presentinvention and their complements can be designed to target ROCK 1. Thenucleobase sequence of the antisense strand of the duplex comprises atleast a portion of an oligonucleotide in Table 1. The ends of thestrands may be modified by the addition of one or more natural ormodified nucleobases to form an overhang. The sense strand of the dsRNAis then designed and synthesized as the complement of the antisensestrand and may also contain modifications or additions to eitherterminus. For example, in one embodiment, both strands of the dsRNAduplex would be complementary over the central nucleobases, each havingoverhangs at one or both termini.

[0142] For example, a duplex comprising an antisense strand having thesequence CGAGAGGCGGACGGGACCG and having a two-nucleobase overhang ofdeoxythymidine(dT) would have the following structure:  cgagaggcggacgggaccgTT Antisense Strand   |||||||||||||||||||TTgctctccgcctgccctggc Complement

[0143] RNA strands of the duplex can be synthesized by methods disclosedherein or purchased from Dharmacon Research Inc., (Lafayette, Colo.).Once synthesized, the complementary strands are annealed. The singlestrands are aliquoted and diluted to a concentration of 50 uM. Oncediluted, 30 uL of each strand is combined with 15 uL of a 5× solution ofannealing buffer. The final concentration of said buffer is 100 mMpotassium acetate, 30 mM HEPES-KOH pH 7.4, and 2 mM magnesium acetate.The final volume is 75 uL. This solution is incubated for 1 minute at90° C. and then centrifuged for 15 seconds. The tube is allowed to sitfor 1 hour at 37° C. at which time the dsRNA duplexes are used inexperimentation. The final concentration of the dsRNA duplex is 20 uM.This solution can be stored frozen (−20° C.) and freeze-thawed up to 5times.

[0144] Once prepared, the duplexed antisense compounds are evaluated fortheir ability to modulate ROCK 1 expression.

[0145] When cells reached 80% confluency, they are treated with duplexedantisense compounds of the invention. For cells grown in 96-well plates,wells are washed once with 200 μL OPTI-MEM-1 reduced-serum medium (GibcoBRL) and then treated with 130 μL of OPTI-MEM-1 containing 12 μg/mLLIPOFECTIN (Gibco BRL) and the desired duplex antisense compound at afinal concentration of 200 nM. After 5 hours of treatment, the medium isreplaced with fresh medium. Cells are harvested 16 hours aftertreatment, at which time RNA is isolated and target reduction measuredby RT-PCR.

Example 6

[0146] Oligonucleotide Isolation

[0147] After cleavage from the controlled pore glass solid support anddeblocking in concentrated ammonium hydroxide at 55° C. for 12-16 hours,the oligonucleotides or oligonucleosides are recovered by precipitationout of 1 M NH₄OAc with >3 volumes of ethanol. Synthesizedoligonucleotides were analyzed by electrospray mass spectroscopy(molecular weight determination) and by capillary gel electrophoresisand judged to be at least 70% full length material. The relative amountsof phosphorothioate and phosphodiester linkages obtained in thesynthesis was determined by the ratio of correct molecular weightrelative to the -16 amu product (+/−32+/−48). For some studiesoligonucleotides were purified by HPLC, as described by Chiang et al.,J. Biol. Chem. 1991, 266, 18162-18171. Results obtained withHPLC-purified material were similar to those obtained with non-HPLCpurified material.

Example 7

[0148] Oligonucleotide Synthesis—96 Well Plate Format

[0149] Oligonucleotides were synthesized via solid phase P(III)phosphoramidite chemistry on an automated synthesizer capable ofassembling 96 sequences simultaneously in a 96-well format.Phosphodiester internucleotide linkages were afforded by oxidation withaqueous iodine. Phosphorothioate internucleotide linkages were generatedby sulfurization utilizing 3,H-1,2 benzodithiole-3-one 1,1 dioxide(Beaucage Reagent) in anhydrous acetonitrile. Standard base-protectedbeta-cyanoethyl-diiso-propyl phosphoramidites were purchased fromcommercial vendors (e.g. PE-Applied Biosystems, Foster City, Calif., orPharmacia, Piscataway, N.J.). Non-standard nucleosides are synthesizedas per standard or patented methods. They are utilized as base protectedbeta-cyanoethyldiisopropyl phosphoramidites.

[0150] Oligonucleotides were cleaved from support and deprotected withconcentrated NH₄OH at elevated temperature (55-60° C.) for 12-16 hoursand the released product then dried in vacuo. The dried product was thenre-suspended in sterile water to afford a master plate from which allanalytical and test plate samples are then diluted utilizing roboticpipettors.

Example 8

[0151] Oligonucleotide Analysis—96-Well Plate Format

[0152] The concentration of oligonucleotide in each well was assessed bydilution of samples and UV absorption spectroscopy. The full-lengthintegrity of the individual products was evaluated by capillaryelectrophoresis (CE) in either the 96-well format (Beckman P/ACE™ MDQ)or, for individually prepared samples, on a commercial CE apparatus(e.g., Beckman P/ACE™ 5000, ABI 270). Base and backbone composition wasconfirmed by mass analysis of the compounds utilizing electrospray-massspectroscopy. All assay test plates were diluted from the master plateusing single and multi-channel robotic pipettors. Plates were judged tobe acceptable if at least 85% of the compounds on the plate were atleast 85% full length.

Example 9

[0153] Cell Culture and Oligonucleotide Treatment

[0154] The effect of antisense compounds on target nucleic acidexpression can be tested in any of a variety of cell types provided thatthe target nucleic acid is present at measurable levels. This can beroutinely determined using, for example, PCR or Northern blot analysis.The following cell types are provided for illustrative purposes, butother cell types can be routinely used, provided that the target isexpressed in the cell type chosen. This can be readily determined bymethods routine in the art, for example Northern blot analysis,ribonuclease protection assays, or RT-PCR.

[0155] T-24 Cells:

[0156] The human transitional cell bladder carcinoma cell line T-24 wasobtained from the American Type Culture Collection (ATCC) (Manassas,Va.). T-24 cells were routinely cultured in complete McCoy's 5A basalmedia (Invitrogen Corporation, Carlsbad, Calif.) supplemented with 10%fetal calf serum (Invitrogen Corporation, Carlsbad, Calif.), penicillin100 units per mL, and streptomycin 100 micrograms per mL (InvitrogenCorporation, Carlsbad, Calif.). Cells were routinely passaged bytrypsinization and dilution when they reached 90% confluence. Cells wereseeded into 96-well plates (Falcon-Primaria #353872) at a density of7000 cells/well for use in RT-PCR analysis.

[0157] For Northern blotting or other analysis, cells may be seeded onto100 mm or other standard tissue culture plates and treated similarly,using appropriate volumes of medium and oligonucleotide.

[0158] A549 Cells:

[0159] The human lung carcinoma cell line A549 was obtained from theAmerican Type Culture Collection (ATCC) (Manassas, Va.). A549 cells wereroutinely cultured in DMEM basal media (Invitrogen Corporation,Carlsbad, Calif.) supplemented with 10% fetal calf serum (InvitrogenCorporation, Carlsbad, Calif.), penicillin 100 units per mL, andstreptomycin 100 micrograms per mL (Invitrogen Corporation, Carlsbad,Calif.). Cells were routinely passaged by trypsinization and dilutionwhen they reached 90% confluence.

[0160] NHDF Cells:

[0161] Human neonatal dermal fibroblast (NHDF) were obtained from theClonetics Corporation (Walkersville, Md.). NHDFs were routinelymaintained in Fibroblast Growth Medium (Clonetics Corporation,Walkersville, Md.) supplemented as recommended by the supplier. Cellswere maintained for up to 10 passages as recommended by the supplier.

[0162] HEK Cells:

[0163] Human embryonic keratinocytes (HEK) were obtained from theClonetics Corporation (Walkersville, Md.). HEKs were routinelymaintained in Keratinocyte Growth Medium (Clonetics Corporation,Walkersville, Md.) formulated as recommended by the supplier. Cells wereroutinely maintained for up to 10 passages as recommended by thesupplier.

[0164] Treatment with Antisense Compounds:

[0165] When cells reached 65-75% confluency, they were treated witholigonucleotide. For cells grown in 96-well plates, wells were washedonce with 100 μL OPTI-MEM™-1reduced-serum medium (InvitrogenCorporation, Carlsbad, Calif.) and then treated with 130 μL ofOPTI-MEM™-1 containing 3.75 μg/mL LIPOFECTIN™ (Invitrogen Corporation,Carlsbad, Calif.) and the desired concentration of oligonucleotide.Cells are treated and data are obtained in triplicate. After 4-7 hoursof treatment at 37° C., the medium was replaced with fresh medium. Cellswere harvested 16-24 hours after oligonucleotide treatment.

[0166] The concentration of oligonucleotide used varies from cell lineto cell line. To determine the optimal oligonucleotide concentration fora particular cell line, the cells are treated with a positive controloligonucleotide at a range of concentrations. For human cells thepositive control oligonucleotide is selected from either ISIS 13920(TCCGTCATCGCTCCTCAGGG, SEQ ID NO: 1) which is targeted to human H-ras,or ISIS 18078, (GTGCGCGCGAGCCCGAAATC, SEQ ID NO: 2) which is targeted tohuman Jun-N-terminal kinase-2 (JNK2). Both controls are2′-O-methoxyethyl gapmers (2-O-methoxyethyls shown in bold) with aphosphorothioate backbone. For mouse or rat cells the positive controloligonucleotide is ISIS 15770, ATGCATTCTGCCCCCAAGGA, SEQ ID NO: 3, a2′-O-methoxyethyl gapmer (2′-O-methoxyethyls shown in bold) with aphosphorothioate backbone which is targeted to both mouse and rat c-raf.The concentration of positive control oligonucleotide that results in80% inhibition of c-H-ras (for ISIS 13920), JNK2 (for ISIS 18078) orc-raf (for ISIS 15770) mRNA is then utilized as the screeningconcentration for new oligonucleotides in subsequent experiments forthat cell line. If 80% inhibition is not achieved, the lowestconcentration of positive control oligonucleotide that results in 60%inhibition of c-H-ras, JNK2 or c-raf mRNA is then utilized as theoligonucleotide screening concentration in subsequent experiments forthat cell line. If 60% inhibition is not achieved, that particular cellline is deemed as unsuitable for oligonucleotide transfectionexperiments. The concentrations of antisense oligonucleotides usedherein are from 50 nM to 300 nM.

Example 10

[0167] Analysis of Oligonucleotide Inhibition of ROCK 1 Expression

[0168] Antisense modulation of ROCK 1 expression can be assayed in avariety of ways known in the art. For example, ROCK 1 mRNA levels can bequantitated by, e.g., Northern blot analysis, competitive polymerasechain reaction (PCR), or real-time PCR (RT-PCR). Real-time quantitativePCR is presently preferred. RNA analysis can be performed on totalcellular RNA or poly(A)+mRNA. The preferred method of RNA analysis ofthe present invention is the use of total cellular RNA as described inother examples herein. Methods of RNA isolation are well known in theart. Northern blot analysis is also routine in the art. Real-timequantitative (PCR) can be conveniently accomplished using thecommercially available ABI PRIS™ 7600, 7700, or 7900 Sequence DetectionSystem, available from PE-Applied Biosystems, Foster City, Calif. andused according to manufacturer's instructions.

[0169] Protein levels of ROCK 1 can be quantitated in a variety of wayswell known in the art, such as immunoprecipitation, Western blotanalysis (immunoblotting), enzyme-linked immunosorbent assay (ELISA) orfluorescence-activated cell sorting (FACS). Antibodies directed to ROCK1 can be identified and obtained from a variety of sources, such as theMSRS catalog of antibodies (Aerie Corporation, Birmingham, Mich.), orcan be prepared via conventional monoclonal or polyclonal antibodygeneration methods well known in the art.

Example 11

[0170] Design of Phenotypic Assays and In Vivo Studies for the Use ofROCK 1 Inhibitors

[0171] Phenotypic Assays

[0172] Once ROCK 1 inhibitors have been identified by the methodsdisclosed herein, the compounds are further investigated in one or morephenotypic assays, each having measurable endpoints predictive ofefficacy in the treatment of a particular disease state or condition.Phenotypic assays, kits and reagents for their use are well known tothose skilled in the art and are herein used to investigate the roleand/or association of ROCK 1 in health and disease. Representativephenotypic assays, which can be purchased from any one of severalcommercial vendors, include those for determining cell viability,cytotoxicity, proliferation or cell survival (Molecular Probes, Eugene,Oreg.; PerkinElmer, Boston, Mass.), protein-based assays includingenzymatic assays (Panvera, LLC, Madison, Wis.; BD Biosciences, FranklinLakes, N.J.; Oncogene Research Products, San Diego, Calif.), cellregulation, signal transduction, inflammation, oxidative processes andapoptosis (Assay Designs Inc., Ann Arbor, Mich.), triglycerideaccumulation (Sigma-Aldrich, St. Louis, Mo.), angiogenesis assays, tubeformation assays, cytokine and hormone assays and metabolic assays(Chemicon International Inc., Temecula, Calif.; Amersham Biosciences,Piscataway, N.J.).

[0173] In one non-limiting example, cells determined to be appropriatefor a particular phenotypic assay (i.e., MCF-7 cells selected for breastcancer studies; adipocytes for obesity studies) are treated with ROCK 1inhibitors identified from the in vitro studies as well as controlcompounds at optimal concentrations which are determined by the methodsdescribed above. At the end of the treatment period, treated anduntreated cells are analyzed by one or more methods specific for theassay to determine phenotypic outcomes and endpoints.

[0174] Phenotypic endpoints include changes in cell morphology over timeor treatment dose as well as changes in levels of cellular componentssuch as proteins, lipids, nucleic acids, hormones, saccharides ormetals. Measurements of cellular status which include pH, stage of thecell cycle, intake or excretion of biological indicators by the cell,are also endpoints of interest.

[0175] Analysis of the geneotype of the cell (measurement of theexpression of one or more of the genes of the cell) after treatment isalso used as an indicator of the efficacy or potency of the ROCK 1inhibitors. Hallmark genes, or those genes suspected to be associatedwith a specific disease state, condition, or phenotype, are measured inboth treated and untreated cells.

[0176] In Vivo Studies

[0177] The individual subjects of the in vivo studies described hereinare warm-blooded vertebrate animals, which includes humans.

[0178] The clinical trial is subjected to rigorous controls to ensurethat individuals are not unnecessarily put at risk and that they arefully informed about their role in the study. To account for thepsychological effects of receiving treatments, volunteers are randomlygiven placebo or ROCK 1 inhibitor. Furthermore, to prevent the doctorsfrom being biased in treatments, they are not informed as to whether themedication they are administering is a ROCK 1 inhibitor or a placebo.Using this randomization approach, each volunteer has the same chance ofbeing given either the new treatment or the placebo.

[0179] Volunteers receive either the ROCK 1 inhibitor or placebo foreight week period with biological parameters associated with theindicated disease state or condition being measured at the beginning(baseline measurements before any treatment), end (after the finaltreatment), and at regular intervals during the study period. Suchmeasurements include the levels of nucleic acid molecules encoding ROCK1 or ROCK 1 protein levels in body fluids, tissues or organs compared topre-treatment levels. Other measurements include, but are not limitedto, indices of the disease state or condition being treated, bodyweight, blood pressure, serum titers of pharmacologic indicators ofdisease or toxicity as well as ADME (absorption, distribution,metabolism and excretion) measurements.

[0180] Information recorded for each patient includes age (years),gender, height (cm), family history of disease state or condition(yes/no), motivation rating (some/moderate/great) and number and type ofprevious treatment regimens for the indicated disease or condition.

[0181] Volunteers taking part in this study are healthy adults (age 18to 65 years) and roughly an equal number of males and femalesparticipate in the study. Volunteers with certain characteristics areequally distributed for placebo and ROCK 1 inhibitor treatment. Ingeneral, the volunteers treated with placebo have little or no responseto treatment, whereas the volunteers treated with the ROCK 1 inhibitorshow positive trends in their disease state or condition index at theconclusion of the study.

Example 12

[0182] RNA Isolation

[0183] Poly(A)+mRNA Isolation

[0184] Poly(A)+mRNA was isolated according to Miura et al., (Clin.Chem., 1996, 42, 1758-1764). Other methods for poly(A)+mRNA isolationare routine in the art. Briefly, for cells grown on 96-well plates,growth medium was removed from the cells and each well was washed with200 μL cold PBS. 60 μL lysis buffer (10 mM Tris-HCl, pH 7.6, 1 mM EDTA,0.5 M NaCl, 0.5% NP-40, 20 mM vanadyl-ribonucleoside complex) was addedto each well, the plate was gently agitated and then incubated at roomtemperature for five minutes. 55 μL of lysate was transferred to Oligod(T) coated 96-well plates (AGCT Inc., Irvine Calif.). Plates wereincubated for 60 minutes at room temperature, washed 3 times with 200 μLof wash buffer (10 mM Tris-HCl pH 7.6, 1 mM EDTA, 0.3 M NaCl). After thefinal wash, the plate was blotted on paper towels to remove excess washbuffer and then air-dried for 5 minutes. 60 μL of elution buffer (5 mMTris-HCl pH 7.6), preheated to 70° C., was added to each well, the platewas incubated on a 90° C. hot plate for 5 minutes, and the eluate wasthen transferred to a fresh 96-well plate.

[0185] Cells grown on 100 mm or other standard plates may be treatedsimilarly, using appropriate volumes of all solutions.

[0186] Total RNA Isolation

[0187] Total RNA was isolated using an RNEASY ₉₆™ kit and bufferspurchased from Qiagen Inc. (Valencia, Calif.) following themanufacturer's recommended procedures. Briefly, for cells grown on96-well plates, growth medium was removed from the cells and each wellwas washed with 200 μL cold PBS. 150 μL Buffer RLT was added to eachwell and the plate vigorously agitated for 20 seconds. 150 μL of 70%ethanol was then added to each well and the contents mixed by pipettingthree times up and down. The samples were then transferred to the RNEASY96™ well plate attached to a QIAVAC™ manifold fitted with a wastecollection tray and attached to a vacuum source. Vacuum was applied for1 minute. 500 μL of Buffer RW1 was added to each well of the RNEASY 96™plate and incubated for 15 minutes and the vacuum was again applied for1 minute. An additional 500 μL of Buffer RW1 was added to each well ofthe RNEASY 96™ plate and the vacuum was applied for 2 minutes. 1 mL ofBuffer RPE was then added to each well of the RNEASY 96™ plate and thevacuum applied for a period of 90 seconds. The Buffer RPE wash was thenrepeated and the vacuum was applied for an additional 3 minutes. Theplate was then removed from the QIAVAC™ manifold and blotted dry onpaper towels. The plate was then re-attached to the QIAVAC™ manifoldfitted with a collection tube rack containing 1.2 mL collection tubes.RNA was then eluted by pipetting 140 μL of RNAse free water into eachwell, incubating 1 minute, and then applying the vacuum for 3 minutes.

[0188] The repetitive pipetting and elution steps may be automated usinga QIAGEN Bio-Robot 9604 (Qiagen, Inc., Valencia Calif.). Essentially,after lysing of the cells on the culture plate, the plate is transferredto the robot deck where the pipetting, DNase treatment and elution stepsare carried out.

Example 13

[0189] Real-time Quantitative PCR Analysis of ROCK 1 mRNA Levels

[0190] Quantitation of ROCK 1 mRNA levels was accomplished by real-timequantitative PCR using the ABI PRISM™ 7600, 7700, or 7900 SequenceDetection System (PE-Applied Biosystems, Foster City, Calif.) accordingto manufacturer's instructions. This is a closed-tube, non-gel-based,fluorescence detection system which allows high-throughput quantitationof polymerase chain reaction (PCR) products in real-time. As opposed tostandard PCR in which amplification products are quantitated after thePCR is completed, products in real-time quantitative PCR are quantitatedas they accumulate. This is accomplished by including in the PCRreaction an oligonucleotide probe that anneals specifically between theforward and reverse PCR primers, and contains two fluorescent dyes. Areporter dye (e.g., FAM or JOE, obtained from either PE-AppliedBiosystems, Foster City, Calif., Operon Technologies Inc., Alameda,Calif. or Integrated DNA Technologies Inc., Coralville, Iowa) isattached to the 5′ end of the probe and a quencher dye (e.g., TAMRA,obtained from either PE-Applied Biosystems, Foster City, Calif., OperonTechnologies Inc., Alameda, Calif. or Integrated DNA Technologies Inc.,Coralville, Iowa) is attached to the 3′ end of the probe. When the probeand dyes are intact, reporter dye emission is quenched by the proximityof the 3′ quencher dye. During amplification, annealing of the probe tothe target sequence creates a substrate that can be cleaved by the5′-exonuclease activity of Taq polymerase. During the extension phase ofthe PCR amplification cycle, cleavage of the probe by Taq polymerasereleases the reporter dye from the remainder of the probe (and hencefrom the quencher moiety) and a sequence-specific fluorescent signal isgenerated. With each cycle, additional reporter dye molecules arecleaved from their respective probes, and the fluorescence intensity ismonitored at regular intervals by laser optics built into the ABI PRISM™Sequence Detection System. In each assay, a series of parallel reactionscontaining serial dilutions of mRNA from untreated control samplesgenerates a standard curve that is used to quantitate the percentinhibition after antisense oligonucleotide treatment of test samples.

[0191] Prior to quantitative PCR analysis, primer-probe sets specific tothe target gene being measured are evaluated for their ability to be“multiplexed” with a GAPDH amplification reaction. In multiplexing, boththe target gene and the internal standard gene GAPDH are amplifiedconcurrently in a single sample. In this analysis, mRNA isolated fromuntreated cells is serially diluted. Each dilution is amplified in thepresence of primer-probe sets specific for GAPDH only, target gene only(“single-plexing”), or both (multiplexing). Following PCR amplification,standard curves of GAPDH and target mRNA signal as a function ofdilution are generated from both the single-plexed and multiplexedsamples. If both the slope and correlation coefficient of the GAPDH andtarget signals generated from the multiplexed samples fall within 10% oftheir corresponding values generated from the single-plexed samples, theprimer-probe set specific for that target is deemed multiplexable. Othermethods of PCR are also known in the art.

[0192] PCR reagents were obtained from Invitrogen Corporation,(Carlsbad, Calif.). RT-PCR reactions were carried out by adding 20 μLPCR cocktail (2.5×PCR buffer minus MgCl₂, 6.6 mM MgCl₂, 375 μM each ofDATP, dCTP, dCTP and dGTP, 375 nM each of forward primer and reverseprimer, 125 nM of probe, 4 Units RNAse inhibitor, 1.25 Units PLATINUM®Taq, 5 Units MuLV reverse transcriptase, and 2.5×ROX dye) to 96-wellplates containing 30 μL total RNA solution (20-200 ng). The RT reactionwas carried out by incubation for 30 minutes at 48° C. Following a 10minute incubation at 95° C. to activate the PLATINUM® Taq, 40 cycles ofa two-step PCR protocol were carried out: 95° C. for 15 seconds(denaturation) followed by 60° C. for 1.5 minutes (annealing/extension).

[0193] Gene target quantities obtained by real time RT-PCR arenormalized using either the expression level of GAPDH, a gene whoseexpression is constant, or by quantifying total RNA using RiboGreen™(Molecular Probes, Inc. Eugene, Oreg.). GAPDH expression is quantifiedby real time RT-PCR, by being run simultaneously with the target,multiplexing, or separately. Total RNA is quantified using RiboGreen™RNA quantification reagent (Molecular Probes, Inc. Eugene, Oreg.).Methods of RNA quantification by RiboGreen™ are taught in Jones, L. J.,et al, (Analytical Biochemistry, 1998, 265, 368-374).

[0194] In this assay, 170 μL of RiboGreen™ working reagent (RiboGreen™reagent diluted 1:350 in 10 mM Tris-HCl, 1 mM EDTA, pH 7.5) is pipettedinto a 96-well plate containing 30 μL purified, cellular RNA. The plateis read in a CytoFluor 4000 (PE Applied Biosystems) with excitation at485 nm and emission at 530 nm.

[0195] Probes and primers to human ROCK 1 were designed to hybridize toa human ROCK 1 sequence, using published sequence information (GenBankaccession number NM_(—)005406.1, incorporated herein as SEQ ID NO:4).For human ROCK 1 the PCR primers were: forward primer:AACGAAGAGACAGAGGTCATGATTC (SEQ ID NO: 5) reverse primer:TTGAGATGCTTCACCTCCTCTTG (SEQ ID NO: 6) and the PCR probe was:FAM-AGATGATTGGAGACCTTCAAGCTCGAATTACATC-TAMRA (SEQ ID NO: 7) where FAM isthe fluorescent dye and TAMRA is the quencher dye. For human GAPDH thePCR primers were:

[0196] forward primer: GAAGGTGAAGGTCGGAGTC(SEQ ID NO:8) reverse primer:GAAGATGGTGATGGGATTTC (SEQ ID NO:9) and the PCR probe was: 5′JOE-CAAGCTTCCCGTTCTCAGCC-TAMRA 3′ (SEQ ID NO: 10) where JOE is thefluorescent reporter dye and TAMRA is the quencher dye.

Example 14

[0197] Northern Blot Analysis of ROCK 1 mRNA Levels

[0198] Eighteen hours after antisense treatment, cell monolayers werewashed twice with cold PBS and lysed in 1 mL RNAZOL™ (TEL-TEST “B” Inc.,Friendswood, Tex.). Total RNA was prepared following manufacturer'srecommended protocols. Twenty micrograms of total RNA was fractionatedby electrophoresis through 1.2% agarose gels containing 1.1%formaldehyde using a MOPS buffer system (AMRESCO, Inc. Solon, Ohio). RNAwas transferred from the gel to HYBONDTM™-N+ nylon membranes (AmershamPharmacia Biotech, Piscataway, N.J.) by overnight capillary transferusing a Northern/Southern Transfer buffer system (TEL-TEST “B” Inc.,Friendswood, Tex.). RNA transfer was confirmed by UV visualization.Membranes were fixed by UV cross-linking using a STRATALINKER™ UVCrosslinker 2400 (Stratagene, Inc, La Jolla, Calif.) and then probedusing QUICKHYB™ hybridization solution (Stratagene, La Jolla, Calif.)using manufacturer's recommendations for stringent conditions.

[0199] To detect human ROCK 1, a human ROCK 1 specific probe wasprepared by PCR using the forward primer AACGAAGAGACAGAGGTCATGATTC (SEQID NO: 5) and the reverse primer TTGAGATGCTTCACCTCCTCTTG (SEQ ID NO: 6).To normalize for variations in loading and transfer efficiency membraneswere stripped and probed for human glyceraldehyde-3-phosphatedehydrogenase (GAPDH) RNA (Clontech, Palo Alto, Calif.).

[0200] Hybridized membranes were visualized and quantitated using aPHOSPHORIMAGER™ and IMAGEQUANT™ Software V3.3 (Molecular Dynamics,Sunnyvale, Calif.). Data was normalized to GAPDH levels in untreatedcontrols.

Example 15

[0201] Antisense Inhibition of Human ROCK 1 Expression by ChimericPhosphorothioate Oligonucleotides having 2′-MOE Wings and a Deoxy Gap

[0202] In accordance with the present invention, a series of antisensecompounds were designed to target different regions of the human ROCK 1RNA, using published sequences (GenBank accession number NM_(—)005406.1,incorporated herein as SEQ ID NO: 4, nucleotides 794383 to 1106859 ofthe sequence with GenBank accession number NT_(—)031911.1, incorporatedherein as SEQ ID NO: 12, and GenBank accession number XM_(—)085795.1,incorporated herein as SEQ ID NO: 13). The compounds are shown inTable 1. “Target site” indicates the first (5′-most) nucleotide numberon the particular target sequence to which the compound binds. Allcompounds in Table 1 are chimeric oligonucleotides (“gapmers”) 20nucleotides in length, composed of a central “gap” region consisting often 2′-deoxynucleotides, which is flanked on both sides (5′ and 3′directions) by five-nucleotide “wings”. The wings are composed of2′-methoxyethyl (2′-MOE)nucleotides. The internucleoside (backbone)linkages are phosphorothioate (P═S) throughout the oligonucleotide. Allcytidine residues are 5-methylcytidines. The compounds were analyzed fortheir effect on human ROCK 1 mRNA levels by quantitative real-time PCRas described in other examples herein. Data are averages from threeexperiments in which A549 cells were treated with the antisenseoligonucleotides of the present invention. The positive control for eachdatapoint is identified in the table by sequence ID number. If present,“N.D.” indicates “no data”. TABLE 1 Inhibition of human ROCK 1 mRNAlevels by chimeric phosphorothioate oligonucleotides having 2′-MOE wingsand a deoxy gap TARGET CONTROL SEQ ID TARGET % SEQ ID SEQ ID ISIS #REGION NO SITE SEQUENCE INHIB NO NO 257383 Start 4 1aaactgtccccagtcgacat 80 14 1 Codon 257384 Coding 4 6tctcaaaactgtccccagtc 89 15 1 257385 Coding 4 43 ggatcccgcagcaggttgtc 5816 1 257386 Coding 4 48 atttgggatcccgcagcagg 66 17 1 257387 Coding 4 53ttccgatttgggatcccgca 79 18 1 257388 Coding 4 58 ttcacttccgatttgggatc 6719 1 257389 Coding 4 82 aatccatccagcaaacaatc 64 20 1 257390 Coding 4 87catccaatccatccagcaaa 72 21 1 257391 Coding 4 122 tcttaaggcaggaaaatcca 8522 1 257392 Coding 4 254 ttgaacttctccaaatgcac 85 23 1 257393 Coding 4362 gatgtccctttcttcccaga 60 24 1 257394 Coding 4 527tacttctgcagtatagaatc 82 25 1 257395 Coding 4 567 gaataaaacccatggaatgg 5526 1 257396 Coding 4 625 gctaacttcaaatgtccaga 70 27 1 257397 Coding 4654 tattcatcttcatacaagta 79 28 1 257398 Coding 4 835ccaaccaaagaatctgcata 91 29 1 257399 Coding 4 872 aagtgaatttttatggttca 7430 1 257400 Coding 4 898 gatatgtcattatcatcagg 57 31 1 257401 Coding 41003 tcatttttgaagaagagatg 73 32 1 257402 Coding 4 1039gctacagtgtctcggagcgt 82 33 1 257403 Coding 4 1044 ctggtgctacagtgtctcgg84 34 1 257404 Coding 4 1072 tcaatgtcactacttaaatc 81 35 1 257405 Coding4 1077 tagtatcaatgtcactactt 85 36 1 257406 Coding 4 1082attactagtatcaatgtcac 73 37 1 257407 Coding 4 1087 tcaaaattactagtatcaat61 38 1 257408 Coding 4 1092 agtcatcaaaattactagta 61 39 1 257409 Coding4 1174 gtaaatcctacaaaaggtag 63 40 1 257410 Coding 4 1182tataatatgtaaatcctaca 21 41 1 257411 Coding 4 1187 attgctataatatgtaaatc19 42 1 257412 Coding 4 1192 ctacgattgctataatatgt 81 43 1 257413 Coding4 1197 agtatctacgattgctataa 63 44 1 257414 Coding 4 1477tggtactcattaattctatg 82 45 1 257415 Coding 4 1535 tgtagaaacttcattttcta58 46 1 257416 Coding 4 1597 ttctcattagcaagctgtga 83 47 1 257417 Coding4 1651 tctgtcctaagtaagtcatt 77 48 1 257418 Coding 4 1693atctctgtgtgactcttcct 93 49 1 257419 Coding 4 1698 tgctcatctctgtgtgactc82 50 1 257420 Coding 4 1703 tgacttgctcatctctgtgt 88 51 1 257421 Coding4 1855 ccaatcatctcagaatcatg 72 52 1 257422 Coding 4 1860ggtctccaatcatctcagaa 92 53 1 257423 Coding 4 1865 ttgaaggtctccaatcatct89 54 1 257424 Coding 4 1955 catgtcttgagcctcttttc 87 55 1 257425 Coding4 2170 gccttctctcgagcttctct 87 56 1 257426 Coding 4 2425aataaagtatttatttcctg 54 57 1 257427 Coding 4 2430 cttccaataaagtatttatt61 58 1 257428 Coding 4 2461 tgagctaactcaaattctaa 81 59 1 257429 Coding4 2466 taagctgagctaactcaaat 75 60 1 257430 Coding 4 2480tctatactgtttcgtaagct 77 61 1 257431 Coding 4 2683 gactcagcttttgtttctgc82 62 1 257432 Coding 4 2688 gctcagactcagcttttgtt 81 63 1 257433 Coding4 2723 aaaatactgttcttccagaa 81 64 1 257434 Coding 4 2755gaagcagctttcttgctttc 67 65 1 257435 Coding 4 2760 ttcttgaagcagctttcttg70 66 1 257436 Coding 4 2765 tctatttcttgaagcagctt 65 67 1 257437 Coding4 2989 acagcctgtgttttaagggt 70 68 1 257438 Coding 4 2994tgttaacagcctgtgtttta 73 69 1 257439 Coding 4 3177 attgcgcttgcatgtcattc80 70 1 257440 Coding 4 3182 taccaattgcgcttgcatgt 77 71 1 257441 Coding4 3187 tcttctaccaattgcgcttg 82 72 1 257442 Coding 4 3404ctgtttcttccagccatatc 75 73 1 257443 Coding 4 3409 acatactgtttcttccagcc78 74 1 257444 Coding 4 3414 ccacaacatactgtttcttc 70 75 1 257445 Coding4 3530 atctccttgggttacaggtc 87 76 1 257446 Coding 4 3535tacacatctccttgggttac 76 77 1 257447 Coding 4 3575 tatctggaatattttaggaa63 78 1 257448 Coding 4 3601 ctacattcaccttcatttgc 88 79 1 257449 Coding4 3769 cttcgacactctagggcagg 88 80 1 257450 Coding 4 3774ggcatcttcgacactctagg 76 81 1 257451 Coding 4 3779 aacatggcatcttcgacact82 82 1 257452 Coding 4 3862 tctcttgctgatgttacatc 79 83 1 257453 Coding4 4009 cggaaagactgatttgcagt 83 84 1 257454 Coding 4 4043actagtttttccagatgtat 61 85 1 257455 exon 12 455 tcctgttcaaacaaacggag 786 1 257456 intron 12 14348 gggagtgaaaaatcccagtc 31 87 1 257457 exon: 12171702 aataacctaccaattgaact 10 88 1 intron junction 257458 intron 12237333 gttggaaacgggaatatcat 5 89 1 257459 exon: 12 302069ggcaacttactctatgtcca 82 90 1 intron junction 257460 3′ UTR 13 844cacgactgacagccattttc 67 91 1

[0203] As shown in Table 1, SEQ ID NOs 14, 15, 16, 17, 18, 19, 20, 21,22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39,40, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59,60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77,78, 79, 80, 81, 82, 83, 84, 85, 90 and 91 demonstrated at least 50%inhibition of human ROCK I expression in this assay and are thereforepreferred. More preferred are SEQ ID Nos 15, 29, and 79. The targetregions to which these preferred sequences are complementary are hereinreferred to as “preferred target segments” and are therefore preferredfor targeting by compounds of the present invention. These preferredtarget segments are shown in Table 2. The sequences represent thereverse complement of the preferred antisense compounds shown inTable 1. “Target site” indicates the first (5′-most) nucleotide numberon the particular target nucleic acid to which the oligonucleotidebinds. Also shown in Table 2 is the species in which each of thepreferred target segments was found. TABLE 2 Sequence and position ofpreferred target segments identified in ROCK 1. TARGET SITE SEQ IDTARGET REV COMP SEQ ID ID NO SITE SEQUENCE OF SEQ ID ACTIVE IN NO 1738414 1 atgtcgactggggacagttt 14 H. sapiens 92 173842 4 6gactggggacagttttgaga 15 H. sapiens 93 173843 4 43 gacaacctgctgcgggatcc16 H. sapiens 94 173844 4 48 cctgctgcgggatcccaaat 17 H. sapiens 95173845 4 53 tgcgggatcccaaatcggaa 18 H. sapiens 96 173846 4 58gatcccaaatcggaagtgaa 19 H. sapiens 97 173847 4 82 gattgtttgctggatggatt20 H. sapiens 98 173848 4 87 tttgctggatggattggatg 21 H. sapiens 99173849 4 122 tggattttcctgccttaaga 22 H. sapiens 100 173850 4 254gtgcatttggagaagttcaa 23 H. sapiens 101 173851 4 362 tctgggaagaaagggacatc24 H. sapiens 102 173852 4 527 gattctatactgcagaagta 25 H. sapiens 103173853 4 567 ccattccatgggttttattc 26 H. sapiens 104 173854 4 625tctggacatttgaagttagc 27 H. sapiens 105 173855 4 654 tacttgtatgaagatgaata28 H. sapiens 106 173856 4 835 tatgcagattctttggttgg 29 H. sapiens 107173857 4 872 tgaaccataaaaattcactt 30 H. sapiens 108 173858 4 898cctgatgataatgacatatc 31 H. sapiens 109 173859 4 1003catctcttcttcaaaaatga 32 H. sapiens 110 173860 4 1039acgctccgagacactgtagc 33 H. sapiens 111 173861 4 1044ccgagacactgtagcaccag 34 H. sapiens 112 173862 4 1072gatttaagtagtgacattga 35 H. sapiens 113 173863 4 1077aagtagtgacattgatacta 36 H. sapiens 114 173864 4 1082gtgacattgatactagtaat 37 H. sapiens 115 173865 4 1087attgatactagtaattttga 38 H. sapiens 116 173866 4 1092tactagtaattttgatgact 39 H. sapiens 117 173867 4 1174ctaccttttgtaggatttac 40 H. sapiens 118 173870 4 1192acatattatagcaatcgtag 43 H. sapiens 119 173871 4 1197ttatagcaatcgtagatact 44 H. sapiens 120 173872 4 1477catagaattaatgagtacca 45 H. sapiens 121 173873 4 1535tagaaaatgaagtttctaca 46 H. sapiens 122 173874 4 1597tcacagcttgctaatgagaa 47 H. sapiens 123 173875 4 1651aatgacttacttaggacaga 48 H. sapiens 124 173876 4 1693aggaagagtcacacagagat 49 H. sapiens 125 173877 4 1698gagtcacacagagatgagca 50 H. sapiens 126 173878 4 1703acacagagatgagcaagtca 51 H. sapiens 127 173879 4 1855catgattctgagatgattgg 52 H. sapiens 128 173880 4 1860ttctgagatgattggagacc 53 H. sapiens 129 173881 4 1865agatgattggagaccttcaa 54 H. sapiens 130 173882 4 1955gaaaagaggctcaagacatg 55 H. sapiens 131 173883 4 2170agagaagctcgagagaaggc 56 H. sapiens 132 173884 4 2425caggaaataaatactttatt 57 H. sapiens 133 173885 4 2430aataaatactttattggaag 58 H. sapiens 134 173886 4 2461ttagaatttgagttagctca 59 H. sapiens 135 173887 4 2466atttgagttagctcagctta 60 H. sapiens 136 173888 4 2480agcttacgaaacagtataga 61 H. sapiens 137 173889 4 2683gcagaaacaaaagctgagtc 62 H. sapiens 138 173890 4 2688aacaaaagctgagtctgagc 63 H. sapiens 139 173891 4 2723ttctggaagaacagtatttt 64 H. sapiens 140 173892 4 2755gaaagcaagaaagctgcttc 65 H. sapiens 141 173893 4 2760caagaaagctgcttcaagaa 66 H. sapiens 142 173894 4 2765aagctgcttcaagaaataga 67 H. sapiens 143 173895 4 2989acccttaaaacacaggctgt 68 H. sapiens 144 173896 4 2994taaaacacaggctgttaaca 69 H. sapiens 145 173897 4 3177gaatgacatgcaagcgcaat 70 H. sapiens 146 173898 4 3182acatgcaagcgcaattggta 71 H. sapiens 147 173899 4 3187caagcgcaattggtagaaga 72 H. sapiens 148 173900 4 3404gatatggctggaagaaacag 73 H. sapiens 149 173901 4 3409ggctggaagaaacagtatgt 74 H. sapiens 150 173902 4 3414gaagaaacagtatgttgtgg 75 H. sapiens 151 173903 4 3530gacctgtaacccaaggagat 76 H. sapiens 152 173904 4 3535gtaacccaaggagatgtgta 77 H. sapiens 153 173905 4 3575ttcctaaaatattccagata 78 H. sapiens 154 173906 4 3601gcaaatgaaggtgaatgtag 79 H. sapiens 155 173907 4 3769cctgccctagagtgtcgaag 80 H. sapiens 156 173908 4 3774cctagagtgtcgaagatgcc 81 H. sapiens 157 173909 4 3779agtgtcgaagatgccatgtt 82 H. sapiens 158 173910 4 3862gatgtaacatcagcaagaga 83 H. sapiens 159 173911 4 4009actgcaaatcagtctttccg 84 H. sapiens 160 173912 4 4043atacatctggaaaaactagt 85 H. sapiens 161 173917 12 302069tggacatagagtaagttgcc 90 H. sapiens 162 173918 13 844gaaaatggctgtcagtcgtg 91 H. sapiens 163

[0204] As these “preferred target segments” have been found byexperimentation to be open to, and accessible for,

0 SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 163 <210> SEQ ID NO 1<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence<220> FEATURE: <223> OTHER INFORMATION: Antisense Oligonucleotide <400>SEQUENCE: 1 tccgtcatcg ctcctcaggg 20 <210> SEQ ID NO 2 <211> LENGTH: 20<212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223>OTHER INFORMATION: Antisense Oligonucleotide <400> SEQUENCE: 2gtgcgcgcga gcccgaaatc 20 <210> SEQ ID NO 3 <211> LENGTH: 20 <212> TYPE:DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHERINFORMATION: Antisense Oligonucleotide <400> SEQUENCE: 3 atgcattctgcccccaagga 20 <210> SEQ ID NO 4 <211> LENGTH: 4065 <212> TYPE: DNA <213>ORGANISM: H. sapiens <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION:(1)...(4065) <400> SEQUENCE: 4 atg tcg act ggg gac agt ttt gag act cgattt gaa aaa atg gac aac 48 Met Ser Thr Gly Asp Ser Phe Glu Thr Arg PheGlu Lys Met Asp Asn 1 5 10 15 ctg ctg cgg gat ccc aaa tcg gaa gtg aattcg gat tgt ttg ctg gat 96 Leu Leu Arg Asp Pro Lys Ser Glu Val Asn SerAsp Cys Leu Leu Asp 20 25 30 gga ttg gat gct ttg gta tat gat ttg gat tttcct gcc tta aga aaa 144 Gly Leu Asp Ala Leu Val Tyr Asp Leu Asp Phe ProAla Leu Arg Lys 35 40 45 aac aaa aat att gac aac ttt tta agc aga tat aaagac aca ata aat 192 Asn Lys Asn Ile Asp Asn Phe Leu Ser Arg Tyr Lys AspThr Ile Asn 50 55 60 aaa atc aga gat tta cga atg aaa gct gaa gat tat gaagta gtg aag 240 Lys Ile Arg Asp Leu Arg Met Lys Ala Glu Asp Tyr Glu ValVal Lys 65 70 75 80 gtg att ggt aga ggt gca ttt gga gaa gtt caa ttg gtaagg cat aaa 288 Val Ile Gly Arg Gly Ala Phe Gly Glu Val Gln Leu Val ArgHis Lys 85 90 95 tcc acc agg aag gta tat gct atg aag ctt ctc agc aaa tttgaa atg 336 Ser Thr Arg Lys Val Tyr Ala Met Lys Leu Leu Ser Lys Phe GluMet 100 105 110 ata aag aga tct gat tct gct ttt ttc tgg gaa gaa agg gacatc atg 384 Ile Lys Arg Ser Asp Ser Ala Phe Phe Trp Glu Glu Arg Asp IleMet 115 120 125 gct ttt gcc aac agt cct tgg gtt gtt cag ctt ttt tat gcattc caa 432 Ala Phe Ala Asn Ser Pro Trp Val Val Gln Leu Phe Tyr Ala PheGln 130 135 140 gat gat cgt tat ctc tac atg gtg atg gaa tac atg cct ggtgga gat 480 Asp Asp Arg Tyr Leu Tyr Met Val Met Glu Tyr Met Pro Gly GlyAsp 145 150 155 160 ctt gta aac tta atg agc aac tat gat gtg cct gaa aaatgg gca cga 528 Leu Val Asn Leu Met Ser Asn Tyr Asp Val Pro Glu Lys TrpAla Arg 165 170 175 ttc tat act gca gaa gta gtt ctt gca ttg gat gca atccat tcc atg 576 Phe Tyr Thr Ala Glu Val Val Leu Ala Leu Asp Ala Ile HisSer Met 180 185 190 ggt ttt att cac aga gat gtg aag cct gat aac atg ctgctg gat aaa 624 Gly Phe Ile His Arg Asp Val Lys Pro Asp Asn Met Leu LeuAsp Lys 195 200 205 tct gga cat ttg aag tta gca gat ttt ggt act tgt atgaag atg aat 672 Ser Gly His Leu Lys Leu Ala Asp Phe Gly Thr Cys Met LysMet Asn 210 215 220 aag gaa ggc atg gta cga tgt gat aca gcg gtt gga acacct gat tat 720 Lys Glu Gly Met Val Arg Cys Asp Thr Ala Val Gly Thr ProAsp Tyr 225 230 235 240 att tcc cct gaa gta tta aaa tcc caa ggt ggt gatggt tat tat gga 768 Ile Ser Pro Glu Val Leu Lys Ser Gln Gly Gly Asp GlyTyr Tyr Gly 245 250 255 aga gaa tgt gac tgg tgg tcg gtt ggg gta ttt ttatac gaa atg ctt 816 Arg Glu Cys Asp Trp Trp Ser Val Gly Val Phe Leu TyrGlu Met Leu 260 265 270 gta ggt gat aca cct ttt tat gca gat tct ttg gttgga act tac agt 864 Val Gly Asp Thr Pro Phe Tyr Ala Asp Ser Leu Val GlyThr Tyr Ser 275 280 285 aaa att atg aac cat aaa aat tca ctt acc ttt cctgat gat aat gac 912 Lys Ile Met Asn His Lys Asn Ser Leu Thr Phe Pro AspAsp Asn Asp 290 295 300 ata tca aaa gaa gca aaa aac ctt att tgt gcc ttcctt act gac agg 960 Ile Ser Lys Glu Ala Lys Asn Leu Ile Cys Ala Phe LeuThr Asp Arg 305 310 315 320 gaa gtg agg tta ggg cga aat ggt gta gaa gaaatc aaa cga cat ctc 1008 Glu Val Arg Leu Gly Arg Asn Gly Val Glu Glu IleLys Arg His Leu 325 330 335 ttc ttc aaa aat gac cag tgg gct tgg gaa acgctc cga gac act gta 1056 Phe Phe Lys Asn Asp Gln Trp Ala Trp Glu Thr LeuArg Asp Thr Val 340 345 350 gca cca gtt gta ccc gat tta agt agt gac attgat act agt aat ttt 1104 Ala Pro Val Val Pro Asp Leu Ser Ser Asp Ile AspThr Ser Asn Phe 355 360 365 gat gac ttg gaa gaa gat aaa gga gag gaa gaaaca ttc cct att cct 1152 Asp Asp Leu Glu Glu Asp Lys Gly Glu Glu Glu ThrPhe Pro Ile Pro 370 375 380 aaa gct ttc gtt ggc aat caa cta cct ttt gtagga ttt aca tat tat 1200 Lys Ala Phe Val Gly Asn Gln Leu Pro Phe Val GlyPhe Thr Tyr Tyr 385 390 395 400 agc aat cgt aga tac tta tct tca gca aatcct aat gat aac aga act 1248 Ser Asn Arg Arg Tyr Leu Ser Ser Ala Asn ProAsn Asp Asn Arg Thr 405 410 415 agc tcc aat gca gat aaa agc ttg cag gaaagt ttg caa aaa aca atc 1296 Ser Ser Asn Ala Asp Lys Ser Leu Gln Glu SerLeu Gln Lys Thr Ile 420 425 430 tat aag ctg gaa gaa cag ctg cat aat gaaatg cag tta aaa gat gaa 1344 Tyr Lys Leu Glu Glu Gln Leu His Asn Glu MetGln Leu Lys Asp Glu 435 440 445 atg gag cag aag tgc aga acc tca aac ataaaa cta gac aag ata atg 1392 Met Glu Gln Lys Cys Arg Thr Ser Asn Ile LysLeu Asp Lys Ile Met 450 455 460 aaa gaa ttg gat gaa gag gga aat caa agaaga aat cta gaa tct aca 1440 Lys Glu Leu Asp Glu Glu Gly Asn Gln Arg ArgAsn Leu Glu Ser Thr 465 470 475 480 gtg tct cag att gag aag gag aaa atgttg cta cag cat aga att aat 1488 Val Ser Gln Ile Glu Lys Glu Lys Met LeuLeu Gln His Arg Ile Asn 485 490 495 gag tac caa aga aaa gct gaa cag gaaaat gag aag aga aga aat gta 1536 Glu Tyr Gln Arg Lys Ala Glu Gln Glu AsnGlu Lys Arg Arg Asn Val 500 505 510 gaa aat gaa gtt tct aca tta aag gatcag ttg gaa gac tta aag aaa 1584 Glu Asn Glu Val Ser Thr Leu Lys Asp GlnLeu Glu Asp Leu Lys Lys 515 520 525 gtc agt cag aat tca cag ctt gct aatgag aag ctg tcc cag tta caa 1632 Val Ser Gln Asn Ser Gln Leu Ala Asn GluLys Leu Ser Gln Leu Gln 530 535 540 aag cag cta gaa gaa gcc aat gac ttactt agg aca gaa tcg gac aca 1680 Lys Gln Leu Glu Glu Ala Asn Asp Leu LeuArg Thr Glu Ser Asp Thr 545 550 555 560 gct gta aga ttg agg aag agt cacaca gag atg agc aag tca att agt 1728 Ala Val Arg Leu Arg Lys Ser His ThrGlu Met Ser Lys Ser Ile Ser 565 570 575 cag tta gag tcc ctg aac aga gagttg caa gag aga aat cga att tta 1776 Gln Leu Glu Ser Leu Asn Arg Glu LeuGln Glu Arg Asn Arg Ile Leu 580 585 590 gag aat tct aag tca caa aca gacaaa gat tat tac cag ctg caa gct 1824 Glu Asn Ser Lys Ser Gln Thr Asp LysAsp Tyr Tyr Gln Leu Gln Ala 595 600 605 ata tta gaa gct gaa cga aga gacaga ggt cat gat tct gag atg att 1872 Ile Leu Glu Ala Glu Arg Arg Asp ArgGly His Asp Ser Glu Met Ile 610 615 620 gga gac ctt caa gct cga att acatct tta caa gag gag gtg aag cat 1920 Gly Asp Leu Gln Ala Arg Ile Thr SerLeu Gln Glu Glu Val Lys His 625 630 635 640 ctc aaa cat aat ctc gaa aaagtg gaa gga gaa aga aaa gag gct caa 1968 Leu Lys His Asn Leu Glu Lys ValGlu Gly Glu Arg Lys Glu Ala Gln 645 650 655 gac atg ctt aat cac tca gaaaag gaa aag aat aat tta gag ata gat 2016 Asp Met Leu Asn His Ser Glu LysGlu Lys Asn Asn Leu Glu Ile Asp 660 665 670 tta aac tac aaa ctt aaa tcatta caa caa cgg tta gaa caa gag gta 2064 Leu Asn Tyr Lys Leu Lys Ser LeuGln Gln Arg Leu Glu Gln Glu Val 675 680 685 aat gaa cac aaa gta acc aaagct cgt tta act gac aaa cat caa tct 2112 Asn Glu His Lys Val Thr Lys AlaArg Leu Thr Asp Lys His Gln Ser 690 695 700 att gaa gag gca aag tct gtggca atg tgt gag atg gaa aaa aag ctg 2160 Ile Glu Glu Ala Lys Ser Val AlaMet Cys Glu Met Glu Lys Lys Leu 705 710 715 720 aaa gaa gaa aga gaa gctcga gag aag gct gaa aat cgg gtt gtt cag 2208 Lys Glu Glu Arg Glu Ala ArgGlu Lys Ala Glu Asn Arg Val Val Gln 725 730 735 att gag aaa cag tgt tccatg cta gac gtt gat ctg aag caa tct cag 2256 Ile Glu Lys Gln Cys Ser MetLeu Asp Val Asp Leu Lys Gln Ser Gln 740 745 750 cag aaa cta gaa cat ttgact gga aat aaa gaa agg atg gag gat gaa 2304 Gln Lys Leu Glu His Leu ThrGly Asn Lys Glu Arg Met Glu Asp Glu 755 760 765 gtt aag aat cta acc ctgcaa ctg gag cag gaa tca aat aag cgg ctg 2352 Val Lys Asn Leu Thr Leu GlnLeu Glu Gln Glu Ser Asn Lys Arg Leu 770 775 780 ttg tta caa aat gaa ttgaag act caa gca ttt gag gca gac aat tta 2400 Leu Leu Gln Asn Glu Leu LysThr Gln Ala Phe Glu Ala Asp Asn Leu 785 790 795 800 aaa ggt tta gaa aagcag atg aaa cag gaa ata aat act tta ttg gaa 2448 Lys Gly Leu Glu Lys GlnMet Lys Gln Glu Ile Asn Thr Leu Leu Glu 805 810 815 gca aag aga tta ttagaa ttt gag tta gct cag ctt acg aaa cag tat 2496 Ala Lys Arg Leu Leu GluPhe Glu Leu Ala Gln Leu Thr Lys Gln Tyr 820 825 830 aga gga aat gaa ggacag atg cgg gag cta caa gat cag ctt gaa gct 2544 Arg Gly Asn Glu Gly GlnMet Arg Glu Leu Gln Asp Gln Leu Glu Ala 835 840 845 gag caa tat ttc tcgaca ctt tat aaa acc cag gta aag gaa ctt aaa 2592 Glu Gln Tyr Phe Ser ThrLeu Tyr Lys Thr Gln Val Lys Glu Leu Lys 850 855 860 gaa gaa att gaa gaaaaa aac aga gaa aat tta aag aaa ata cag gaa 2640 Glu Glu Ile Glu Glu LysAsn Arg Glu Asn Leu Lys Lys Ile Gln Glu 865 870 875 880 cta caa aat gaaaaa gaa act ctt gct act cag ttg gat cta gca gaa 2688 Leu Gln Asn Glu LysGlu Thr Leu Ala Thr Gln Leu Asp Leu Ala Glu 885 890 895 aca aaa gct gagtct gag cag ttg gcg cga ggc ctt ctg gaa gaa cag 2736 Thr Lys Ala Glu SerGlu Gln Leu Ala Arg Gly Leu Leu Glu Glu Gln 900 905 910 tat ttt gaa ttgacg caa gaa agc aag aaa gct gct tca aga aat aga 2784 Tyr Phe Glu Leu ThrGln Glu Ser Lys Lys Ala Ala Ser Arg Asn Arg 915 920 925 caa gag att acagat aaa gat cac act gtt agt cgg ctt gaa gaa gca 2832 Gln Glu Ile Thr AspLys Asp His Thr Val Ser Arg Leu Glu Glu Ala 930 935 940 aac agc atg ctaacc aaa gat att gaa ata tta aga aga gag aat gaa 2880 Asn Ser Met Leu ThrLys Asp Ile Glu Ile Leu Arg Arg Glu Asn Glu 945 950 955 960 gag cta acagag aaa atg aag aag gca gag gaa gaa tat aaa ctg gag 2928 Glu Leu Thr GluLys Met Lys Lys Ala Glu Glu Glu Tyr Lys Leu Glu 965 970 975 aag gag gaggag atc agt aat ctt aag gct gcc ttt gaa aag aat atc 2976 Lys Glu Glu GluIle Ser Asn Leu Lys Ala Ala Phe Glu Lys Asn Ile 980 985 990 aac act gaacga acc ctt aaa aca cag gct gtt aac aaa ttg gca gaa 3024 Asn Thr Glu ArgThr Leu Lys Thr Gln Ala Val Asn Lys Leu Ala Glu 995 1000 1005 ata atgaat cga aaa gat ttt aaa att gat aga aag aaa gct aat aca 3072 Ile Met AsnArg Lys Asp Phe Lys Ile Asp Arg Lys Lys Ala Asn Thr 1010 1015 1020 caagat ttg aga aag aaa gaa aag gaa aat cga aag ctg caa ctg gaa 3120 Gln AspLeu Arg Lys Lys Glu Lys Glu Asn Arg Lys Leu Gln Leu Glu 1025 1030 10351040 ctc aac caa gaa aga gag aaa ttc aac cag atg gta gtg aaa cat cag3168 Leu Asn Gln Glu Arg Glu Lys Phe Asn Gln Met Val Val Lys His Gln1045 1050 1055 aag gaa ctg aat gac atg caa gcg caa ttg gta gaa gaa tgtgca cat 3216 Lys Glu Leu Asn Asp Met Gln Ala Gln Leu Val Glu Glu Cys AlaHis 1060 1065 1070 agg aat gag ctt cag atg cag ttg gcc agc aaa gag agtgat att gag 3264 Arg Asn Glu Leu Gln Met Gln Leu Ala Ser Lys Glu Ser AspIle Glu 1075 1080 1085 caa ttg cgt gct aaa ctt ttg gac ctc tcg gat tctaca agt gtt gct 3312 Gln Leu Arg Ala Lys Leu Leu Asp Leu Ser Asp Ser ThrSer Val Ala 1090 1095 1100 agt ttt cct agt gct gat gaa act gat ggt aacctc cca gag tca aga 3360 Ser Phe Pro Ser Ala Asp Glu Thr Asp Gly Asn LeuPro Glu Ser Arg 1105 1110 1115 1120 att gaa ggt tgg ctt tca gta cca aataga gga aat atc aaa cga tat 3408 Ile Glu Gly Trp Leu Ser Val Pro Asn ArgGly Asn Ile Lys Arg Tyr 1125 1130 1135 ggc tgg aag aaa cag tat gtt gtggta agc agc aaa aaa att ttg ttc 3456 Gly Trp Lys Lys Gln Tyr Val Val ValSer Ser Lys Lys Ile Leu Phe 1140 1145 1150 tat aat gac gaa caa gat aaggag caa tcc aat cca tct atg gta ttg 3504 Tyr Asn Asp Glu Gln Asp Lys GluGln Ser Asn Pro Ser Met Val Leu 1155 1160 1165 gac ata gat aaa ctg tttcac gtt aga cct gta acc caa gga gat gtg 3552 Asp Ile Asp Lys Leu Phe HisVal Arg Pro Val Thr Gln Gly Asp Val 1170 1175 1180 tat aga gct gaa actgaa gaa att cct aaa ata ttc cag ata cta tat 3600 Tyr Arg Ala Glu Thr GluGlu Ile Pro Lys Ile Phe Gln Ile Leu Tyr 1185 1190 1195 1200 gca aat gaaggt gaa tgt aga aaa gat gta gag atg gaa cca gta caa 3648 Ala Asn Glu GlyGlu Cys Arg Lys Asp Val Glu Met Glu Pro Val Gln 1205 1210 1215 caa gctgaa aaa act aat ttc caa aat cac aaa ggc cat gag ttt att 3696 Gln Ala GluLys Thr Asn Phe Gln Asn His Lys Gly His Glu Phe Ile 1220 1225 1230 cctaca ctc tac cac ttt cct gcc aat tgt gat gcc tgt gcc aaa cct 3744 Pro ThrLeu Tyr His Phe Pro Ala Asn Cys Asp Ala Cys Ala Lys Pro 1235 1240 1245ctc tgg cat gtt ttt aag cca ccc cct gcc cta gag tgt cga aga tgc 3792 LeuTrp His Val Phe Lys Pro Pro Pro Ala Leu Glu Cys Arg Arg Cys 1250 12551260 cat gtt aag tgc cac aga gat cac tta gat aag aaa gag gac tta att3840 His Val Lys Cys His Arg Asp His Leu Asp Lys Lys Glu Asp Leu Ile1265 1270 1275 1280 tgt cca tgt aaa gta agt tat gat gta aca tca gca agagat atg ctg 3888 Cys Pro Cys Lys Val Ser Tyr Asp Val Thr Ser Ala Arg AspMet Leu 1285 1290 1295 ctg tta gca tgt tct cag gat gaa caa aaa aaa tgggta act cat tta 3936 Leu Leu Ala Cys Ser Gln Asp Glu Gln Lys Lys Trp ValThr His Leu 1300 1305 1310 gta aag aaa atc cct aag aat cca cca tct ggtttt gtt cgt gct tcc 3984 Val Lys Lys Ile Pro Lys Asn Pro Pro Ser Gly PheVal Arg Ala Ser 1315 1320 1325 cct cga acg ctt tct aca aga tcc act gcaaat cag tct ttc cgg aaa 4032 Pro Arg Thr Leu Ser Thr Arg Ser Thr Ala AsnGln Ser Phe Arg Lys 1330 1335 1340 gtg gtc aaa aat aca tct gga aaa actagt taa 4065 Val Val Lys Asn Thr Ser Gly Lys Thr Ser * 1345 1350 <210>SEQ ID NO 5 <211> LENGTH: 25 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: PCR Primer <400>SEQUENCE: 5 aacgaagaga cagaggtcat gattc 25 <210> SEQ ID NO 6 <211>LENGTH: 23 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220>FEATURE: <223> OTHER INFORMATION: PCR Primer <400> SEQUENCE: 6ttgagatgct tcacctcctc ttg 23 <210> SEQ ID NO 7 <211> LENGTH: 34 <212>TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHERINFORMATION: PCR Probe <400> SEQUENCE: 7 agatgattgg agaccttcaagctcgaatta catc 34 <210> SEQ ID NO 8 <211> LENGTH: 19 <212> TYPE: DNA<213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHERINFORMATION: PCR Primer <400> SEQUENCE: 8 gaaggtgaag gtcggagtc 19 <210>SEQ ID NO 9 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: PCR Primer <400>SEQUENCE: 9 gaagatggtg atgggatttc 20 <210> SEQ ID NO 10 <211> LENGTH: 20<212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223>OTHER INFORMATION: PCR Probe <400> SEQUENCE: 10 caagcttccc gttctcagcc 20<210> SEQ ID NO 11 <220> FEATURE: <400> SEQUENCE: 11 000 <210> SEQ ID NO12 <211> LENGTH: 312477 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 31186-31258 <223>OTHER INFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY:misc_feature <222> LOCATION: 39181 <223> OTHER INFORMATION: n = A,T,C orG <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 39183<223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY:misc_feature <222> LOCATION: 39464-39563 <223> OTHER INFORMATION: n =A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:51426-51525 <223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE: <221>NAME/KEY: misc_feature <222> LOCATION: 56648 <223> OTHER INFORMATION: n= A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:56710-56809 <223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE: <221>NAME/KEY: misc_feature <222> LOCATION: 65039-65138 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 73415-73514 <223> OTHER INFORMATION: n = A,T,C or G<220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 79644-79743<223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY:misc_feature <222> LOCATION: 86851-86950 <223> OTHER INFORMATION: n =A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:92371-92470 <223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE: <221>NAME/KEY: misc_feature <222> LOCATION: 97243-97342 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 102609-102708 <223> OTHER INFORMATION: n = A,T,C or G<220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:106849-106948 <223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE:<221> NAME/KEY: misc_feature <222> LOCATION: 113142-113241 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 117089-117188 <223> OTHER INFORMATION: n = A,T,C or G<220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:121444-121543 <223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE:<221> NAME/KEY: misc_feature <222> LOCATION: 126722-126721 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 130441-130541 <223> OTHER INFORMATION: n = A,T,C or G<220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:135160-135259 <223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE:<221> NAME/KEY: misc_feature <222> LOCATION: 135692 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 138860-138959 <223> OTHER INFORMATION: n = A,T,C or G<220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:141856-141955 <223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE:<221> NAME/KEY: misc_feature <222> LOCATION: 144205-144304 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 146475-146574 <223> OTHER INFORMATION: n = A,T,C or G<220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:147608-147707 <223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE:<221> NAME/KEY: misc_feature <222> LOCATION: 149158-149257 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 160337-160436 <223> OTHER INFORMATION: n = A,T,C or G<220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:193431-193530 <223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE:<221> NAME/KEY: misc_feature <222> LOCATION: 204369 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 227937 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 227963-228062<223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY:misc_feature <222> LOCATION: 243193-243292 <223> OTHER INFORMATION: n =A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:245887-245986 <223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE:<221> NAME/KEY: misc_feature <222> LOCATION: 263803-263902 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 266546-266645 <223> OTHER INFORMATION: n = A,T,C or G<220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION:267821-267920 <223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE:<221> NAME/KEY: misc_feature <222> LOCATION: 275949-276048 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 280464 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 280587 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 281830 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 281920 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 281923 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 282024 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 282032 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 282068 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 282084 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 282142 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 282161 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 282174 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 282196 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 282206 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 282209 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 282225 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 283014 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 283066 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 283879 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 284048 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 284636 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 284724 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 284740 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 284751 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 284786 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 284882 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 284883 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 284953 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 284987 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 285011 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 285187 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 285194 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 285217 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 285276 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 286043 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 286059 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 286672 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 287007 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 287508 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 287510 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 287528 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 287577 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 287712 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 287999 <223> OTHERINFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY: misc_feature<222> LOCATION: 288244 <223> OTHER INFORMATION: n = A,T,C or G <220>FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 289257-289356<223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY:misc_feature <222> LOCATION: 303061 <223> OTHER INFORMATION: n = A,T,Cor G <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 303104<223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY:misc_feature <222> LOCATION: 303156 <223> OTHER INFORMATION: n = A,T,Cor G <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: 303196<223> OTHER INFORMATION: n = A,T,C or G <220> FEATURE: <221> NAME/KEY:misc_feature <222> LOCATION: 303294 <223> OTHER INFORMATION: n = A,T,Cor G <400> SEQUENCE: 12 gctcaatgct ggtacctgcc gacgaaaagg cttcttgcctaaaaaagaac ctattgcccc 60 accctaacag caacaaggag aacaagcact ctctcctcgtcagaagtctc tggactggct 120 gcaacccaga ggaaacagag ctggggcatc tgccgggacagcgatcagaa gcagccagag 180 ctccgcctcc gtccggacct ccaaggccgc tccagagccctgcatggctc ctcagagccg 240 gccaaggcga gaaccttgcc ggcagctgct tacccgcactcggctcctcc cagcggtccg 300 ccttctccac cgcctgcccg caaggccccg cccacctcaccgccccgccc caccccaccc 360 tgcggaggcg acagagtgcg cgcgcgcgcg cggggtcccggctggttccc cttccgagcg 420 tccgcgcccc gcatgcgcag tctgccccgg cggtctccgtttgtttgaac aggaaggcgg 480 acatattagt ccctctcagc ccccctcgcc ccaccccccaggcattcgcc gccgcgactc 540 gccctttccc cggctgggac cgcagcccct cccagaagctcccccatcag cagccgccgg 600 gacccaacta tcgtcttcct cttcgcccgc tctccagcctttcctctgct aagtctccat 660 cgggcatcga cctcgccctg ccccaccgga caccgtagcagcagccccag cagcgacggg 720 acaaaatggg agagtgaggc tgtcctgcgt ggaccagctcgtggccgaga ctgatcggtg 780 cgtcgggccg ggccgagtag agccggggac gcggggctagaccgtctaca gcgcctctga 840 gcggagcggg cccggcccgt ggcccgagcg gcggccgcagctggcacagc tcctcacccg 900 ccctttgctt tcgcctttcc tcttctccct cccttgttgcccggagggag tctccaccct 960 gcttctcttt ctctacccgc tcctgcccat ctcgggacggggacccctcc atggcgacgg 1020 cggccggggc ccgctagact gaagcacctc gccggagcgacgaggctggt ggcgacggcg 1080 ctgtcggctg tcgtgagggg ctgccgggtg ggatgcgactttgggcgtcc gagcggctgt 1140 gggtcgctgt tgcccccggc ccggggtctg gagagcggaggtcccctcag tgaggggaag 1200 acgggggaac cgggcgcacc tggtgaccct gaggttccggctcctccgcc ccgcggctgc 1260 gaacccaccg cggaggaagt tggttgaaat tgctttccgctgctggtgct ggtaagaggg 1320 cattgtcaca gcagcagcaa catgtcgact ggggacagttttgagactcg atttgaaaaa 1380 atggacaacc tgctgcggga tcccaaatcg gaagtgaattcggattgttt gctggtgagt 1440 agcgctgttc tcttttgctt gtctggggtt ttgcatgtctttccttcctc ttgcattagt 1500 ctggttcgct gcaaaagtct caggtgtgca taatcgtcttggaaaatgct agatttctgt 1560 ttcctgaaga atcttggagt accattaaca tctcatggtgtggtcctatt tgccatttga 1620 ctggctctgt actaactact ctgaactttt aattctgactcagttcatga gtacccgaaa 1680 gatacttaca aactgctatt gcaggcatcc tataaaaagttatcacgtca gagttactgc 1740 aatgtataca gtttgttgaa tggcgatcgt agtaattaatgttaatcttt ctgatccgtt 1800 tgacagttaa attggaaaga ctactttgca ttttttttcctttttgcgat ttagctccta 1860 ggggtttagt ttttagcctt gaaaatgata acggagaattgctattgagg aagtgatttt 1920 tgagccaaaa tttcataggt gatttgagtt tgaaggaactttttgctaaa ctattgtaaa 1980 aaaccaagat aattggtcat aggtaattac agtgttaacttattttattg tattattggt 2040 tacaaaagta cgtaccatac agttattgta actgtctttttggtggtgtg gcacttaaca 2100 ttgtcacttg taaaacattc taaagagggt tttatgacatgaaaaaccac aatcccattt 2160 taaaaatagt taagtggttg ataatacagt tatctgtaaataattggaat tcttaggttt 2220 gatacatttc ctctgtaaat gtatcaaagt tgttcagagtttctgaacaa ctaagggtta 2280 tataatattt cttggtccaa acaaaaccac ataatctctaaaataaggtt tcagggagca 2340 tgtatatgga caggatttca caatgtcttc ggtgaaataatgttagatat gctcaatttt 2400 ggggattaag attaaggaaa caaaataata ccattcaaagaaataataca attcaaaaat 2460 cgcatcttga atcaaaggaa aattaatgta gattgtttaaaattaacgtg catttcagtt 2520 actctaaatt tctgtttatt tgtatttttc cacgtgtatgggttttacaa caagcttccc 2580 aaagctttca gaattgttgt aagcatccct gaccaggaaataagactatt gtgggttgtt 2640 tgaaatgtct caccacatta tggatgctct ttatattgagagatagtaaa gaaggttgat 2700 gcgtaataga ttttaaaaca ttttattaaa cgagataagtggaaggaatt agaatatatc 2760 ttgtagtaag gatttctata acttctaaga agtctgatttatcttttaat atttatccag 2820 ttttcatata tttttgattt tattttgctt ttcgatctccttattacatg agtggtgtta 2880 tagggagggt ttagtagctt tctttttctt taaatcatcttctggcctct tgctatagct 2940 tcatttggtg gggaccttta tttacatgtg gaggatacaaggaaatataa gtcatgactc 3000 tttccctgag agtccaatct atttaatgga agacaaaaccttgtctcaca tatatacaca 3060 gaaacaaagc taaacacttc acagattgag aaaatgtgataaattccaga tgattgttta 3120 gataatgtat gttcttggaa attgagagaa agaagtcactgtgagttgag agagccaaag 3180 tatgttttat aatggatgga taggatttag gtagagggtactcaagagga gtggaggata 3240 acgtgaatag agggtggaga catgactggc tatgacatgtttggggaaac aaggaagata 3300 aggttggaag gaaatattag cgttagattg tggaggatttgattggtagg ctaaggattt 3360 tggattgcct ttacactgtt gtggctatta aatatttataaataagaccg tgctaggatg 3420 aatgaagaag atgaatctgg tgtgtggaag atgggttagcatgggaaaag attggagaga 3480 cagtgagatt ggacagtttt ttaaaattgt tattattgtaatagtccaga aatgattgaa 3540 gcccaaaatt ggggcatgag aatgagaaaa gaagaattaatggaacttta ttggcaggga 3600 aaagatgggg aaccaaagag agaggcaata gtcagatgactctaaagagg ttgagcctgg 3660 attataggga gaatggtaaa aatagcaaag tcaggtttggaaattgcttg tgaagaatag 3720 gcctacagat aaattcaatt ttagattcgt tgtgtttgaggggatagtgg agcaatcatg 3780 agaaaatgac ctgtagactg ttggagatac agaactgagctcataattac agataatgat 3840 ttagaagtca tttgcacaaa ggtgcaagtt gatataatggtaaagggaga aacaatggag 3900 ggaaaaatga gggtgaaaga tgaaggttga acaaagaggaagagtggagg aggtaagcca 3960 tcaaggcctc cagagttagg acagccaggt atcatggagcccaacacttc taaaacaaca 4020 ttttgttaaa gagaatagtc aacagacaga tttggttgttttttcccctt caaatcttat 4080 aatcttgtcc tctttttctg tttagttcca cagctgtttattgatagctg ttactgaaat 4140 tttattaccc cgtcaatctg aacttggtaa agactgaattttcctttttt tttttctgag 4200 atggaatctc actctgttgc ccaggctgga gtgcagtggcgcgatcttgg ctcactgcaa 4260 cctccgactc ctgggttcga gtgattctcc tgcctcagcccccacgagta gttgggatta 4320 cacgcgtgca ctaccacgcc tggctaattt ttgtagttttagtagagatg gggtttcacc 4380 atgttggcca ggctggtctt gtactcctta ccccaaggaatccgcctgcc ttggcctccc 4440 gaagtactgg gattacaggc gtgagccacc gtgcctggcccaagactgaa tttcctttag 4500 agacaaaaaa tcctttttct gggtttgtag tatctagcatgaattttctt cgcatgatgt 4560 tttatgttta acattgagca ttcgttcgtt cattcattcattgtgtcatt cagggtgacc 4620 tatcaggtga cttcagcttt ttcagtgagt ctgttcttgttcttccctat ctggactttg 4680 gacctctcat gtattatagg aacaaaactc cttttacattggttgccttt ttctgttatt 4740 ttgacttttg ttgatttgtg taactgatac ttacttattagtgtgtatta tgtgccacgt 4800 gcagtccttt agttctagag ttgtttttgt aatcccaaaaataaggtttt caaattgttt 4860 cttctctacc tcctttacta gattcatgct tagaaatggaaacttccaaa tcttttggtt 4920 tcagaaattt gctggtgtat gtccttttaa actaccttacctactaaaca gcttttttgt 4980 tgagacacta atgatgaata gcatccgtaa tctgaaatttgaagtgctca aaatctgaaa 5040 ctttttgagt gctgacatga cgccataagt ggaaaatccacctgaccttg tgagatgggt 5100 tgcagtcaaa atgcagtcag aactttgttt catgcacaaaattattaaaa tattgcaaaa 5160 aataaccttc aggttgttgt gtataaggta tgtatgagacgtaaatgaat ttcttattta 5220 gacttggtcc ccatccccag ggtatcttat gtatatgcaaatattttaaa atctgaaaaa 5280 gtctgcagtc tgaaacactt ctagttccaa gcattttaaacaagggatac tcaacctgca 5340 gtagtttaga ataatgtagc tggaagaact ctgataagtggctatccaat cttttctcga 5400 acacctgtaa tttttttcaa gaactaagta agttcaatcttcatatcatg tataaatgct 5460 aaaatagtct gcaaatacta agatacatta tgataaccattcacttgttt cctagagcat 5520 tcaaattagt caactttggt tttctgtagg tcagattctttccagtgtcc taatggttgt 5580 ctttcttact gtccatgtta cagtttataa ggattatcgggagctatttc tcttttccgg 5640 acttccttat cttctaatta ttttgtctag ggcctgtaataatttctcag tgattttgag 5700 tacctttgaa aacagaataa gggatggagt ttagttaagtctatttaatc tttgcagagg 5760 aaaaaatgct tatgatttgt aaattaaata ggaaaaacaaggccgggtgc agtggctcgc 5820 tcctggaggg ctgaggccag agaattgctt gagcctaggacggggaggtt gcagtgagcc 5880 gagattgtgc cactgcactc cagcctggac gacacagtgagaacttgtct caaaaaaaag 5940 gaaaaacaaa aatggttggg agggttatag atacatttgttttgaagttt gacatacttt 6000 tcatgaaaat atcttgcttg tagcatattt attggcacatcgcttcatga tgcctgttaa 6060 gaacatgacc tttccacttc tcttctggct ggtggaggtgtagcaacggg tgagcggagg 6120 agacagaagg gaaccagtat tagtcaaata gtgcctgttgagttctaaca ctgtgctgtt 6180 tgtcactact atttcttatt agggagtttt tcagaatgtggaagataaat gtaaatgaaa 6240 tttctggtgt tagtctttga gtttttcttc ctttaaattatcttcctggg ctctcttgac 6300 ctagtgcttt cccatctttt cattcttctt tttttcttttcctcaatctt tggcctatca 6360 tacttatctt tgtcttcact ctcttgaaga attcatccattcttacagct tcaattatta 6420 ttatctctat gtacatgata cccaaatctt tatttttagtcttttgtttc aaaccttaaa 6480 tatctgactg ccttgtaagc atttctactt ggatgtcatgttagtctcaa atccctcata 6540 ttccaaaaca agcttcctcc cataaactta tctgttttgtcattaattcc actattgtcc 6600 ctgtcgctaa actgaaattt ttgttgtgaa cttttttgacagtgttagtc cacacattgg 6660 gttattacag cagattttgg gtagcatttt cgaatctggattattgctgt ctaatcccct 6720 gtatgccttg ccagttgaat tgatctgttt tgggggtatattactcttgg aatgaaaaga 6780 cctgaggtca ttaaatcttt gagccttaaa gttgtatctctgccagtgtt cctagtttgt 6840 gagttaatag gtgttattag tatcaattgc atatctggttagtttggacc agactttttt 6900 ggatgttagt atgttgcagg aacttctttt aaaaaaacaagttactctcc taattcattt 6960 ttattcattc agcaaatctt tgtatagggc ctaaacttggattgaaatct aggctctgac 7020 atgtatttgc tgaaatattt tagggagtta ttttaacctctcaatgctgt ttcatcttca 7080 gaaaaataag aggggataaa agtactagat tgttgtaagaattgaataat atagtcaaag 7140 caactatcat agcatttggt atttagtaag catttgtaatattgtcatat ttataatatt 7200 tctgttatat actagtcagt gtgcttagtc tagaagttgatatttattgt cgtctctaag 7260 tacagagtaa tttatctggc acctcaagcc gctcataaattcataatcta gctgtgcgtt 7320 attgacgtct aagccaattg taactgaaaa ttgagacatttattaataga ttcaataaat 7380 ggggaagatc ttttagagac tgtttatttg tttagttgtgatttataata tggtatatac 7440 tttgtgccaa gggtttagtt ggatataaaa ggcaattgggaactaattta tggagattga 7500 gttgttatct tgttctcttt ctgtgtgtat cttttatactttcagtgtgt gttctgtaaa 7560 taggccctct tgcactacag gcaccctgtg ggtgtgtagagacgtggctc agagtaacgg 7620 ctgagatatg aattggttaa ttcattaatc ttttgagtcagaaatgtttt ttttccgccc 7680 agtccgaagt gccgtggcgc tatcttggct tactgcaagctccacctcct gggttcacgc 7740 cattctcctg cctcagcctc ctgagtagct gggactacaggcgcccgcca ccgcacccgg 7800 ctaatttttt gtatttttag tagagacagg gtttcaccgtgttagccagg atggtctcga 7860 tctcctggcc tcgtgatccg cccgcctcag cctcccacagtgctgggatt acaggcatga 7920 gcgccgcacc ctgcccagaa atgtttttaa tccagaagaaggaactacat ataaaatttt 7980 atgttgaata aagagtaaat tctcagtttt agattagactttgcataact ttacaccagt 8040 tttgcttcac gattataagt tttggtgtta tggatatatggtgaaaacta cagattctat 8100 tacgataatt gatagaaaaa cccacttatc aggaaatattatatatagag ttctattgct 8160 gaagataatc caatagctct acttctccat tcctcatccccactaaaaag ggggtatatc 8220 catttagttt aagtccacag tatagctgtt ggatacgacacacatgtgtt caagaagatg 8280 aacaggtagt actgcaattc acaatggaaa gtattcttaaaaatatgcca ttagtgtggt 8340 gtgaaaagaa cactggactg aaaattcaga gcatagtcttgttttgctat taaatctctg 8400 tgtaatgaag tacatcattt ttctcatcta ggcttcagtttccctatttg tagaacaaag 8460 agttggacca gatagttgct gatgtctctt gtgtctctgaaaattctatg ataggcctgg 8520 catggtggtc tgtaatccca gcacttttgg aggccaaggtgcaagtatcc cttgagccca 8580 ggagtttgag accggcctgg gcaacagaga gggacctcggctctacaaaa aaaataaaaa 8640 tattacctgg gtgtagtctc agctacttgg gaggttgaggtaggaagatc gcttgagccc 8700 aggagattga ggctgcagtg agccatgttg gtgccacggcactctagcct ggttgtcaaa 8760 gtgagactct gtctcaaaaa aaaaaaataa taaaataaaatttattaaac aaaatctatg 8820 agactttgtt tattacactt caattaaaac ttcaaaaaattgtctttgat ttattattta 8880 agaaataaaa cttaaacagt taaatgatgt tggggtagaaatgtaagact gtcaaccatc 8940 tttagtaagt gctttttttg gaactagttc ctaaattaatttcaatagtt atatttgcca 9000 ttattttttg aatttgtgac ttacgttcat aattgtaggggtttgtactt tctgaacata 9060 ggaagtgtaa ttttttattt gtatatctat taaaatgtaaagatatgatt ttcttctcta 9120 gtatacttgc cctctctgtt tgggattatg aaatatagagtgtattttta ttacagttcc 9180 taaacaatta ataggaaggt agatgttcaa attaacgtccaaagtctgat atctcaggga 9240 ggtgagagtt tatcttatac ttgtgttata ttaagggtaggccaacctgt gcccctctta 9300 attgatccat acttctgata ccagattttt ggcaagaaattgaaaatttt ctcagattgt 9360 tgtcttgaac tacttttttt ttttttgaga cagggtctggccctgtcacc caggctgaag 9420 tgtggtggca tgatctcagc tcactgaagc ctccacttcccgagctgaag ccattcttct 9480 gcctcagcct cttgagtaac tgggactaca ggcaagcgccaccatgcctg gctaattttt 9540 gtattttttt tggtagagat ggggttttgc catgttgcccaggctggtct caagtgagcc 9600 attggcctca gcttcccaaa gtgtaaggat tacaggcgtgagccacagca cccagccctc 9660 aaactaattt tgaaacagtg gactgcaatt aactttcctttttctctttt ctgttattca 9720 ttcattcatt tgacaaatgt ttatttggtg tgtactgtgtgctaggaatt ctgctatgca 9780 atggaacacc atggtgagta aaaacaaaca tgtcccactttcatggaatt tatagtgtca 9840 ggaaaaggat taattaaatg tctatcacac taagataggagtggggtatg gtgaagggca 9900 ggtaagagat atgatcatga ctttccttta aggtttggtttacttgtagt aaagcaatta 9960 agtgtatagt tttcagatat gaggctgtag attgtatcagattcacttga ggcacttgtt 10020 aaaaatggaa tctgggatct tgcgtagaca aacaaaatcacaattttttg ggatgattct 10080 taggatttgc atgagatgtg gattcttaga cacactcaagtttgaaaact gtagttacag 10140 tggaaagaat gtgaattttc attctgtttg gtaattttatgataaaaatt tcaaatatac 10200 gcagaagtag ggaaaatagg atagtgaact cctgttactcagattttgca gttttcaaaa 10260 tgtcatcaca tttgatttca ttaatcaccc tttttcttaccaaaatattt taaagtaaat 10320 tccatatatt gtcatttcat ccttacatac ttcaataagcatctttgaaa aaaaaaaagg 10380 acattttctt acataatcat aatggccatt atcatgcctagcaaaattaa aagtgctttg 10440 gtatcattaa ttcccagttt atattcatat ttctgattgtctaaaacagt gtctatttta 10500 cagttgcttt attagaatct ggatccaaag tccaaatgttgcttttcttt ggttaggtcc 10560 tttaagagtc ttttaagtct cttaatatag agaaatctcattcccccaac ttctccaact 10620 ccccttcccc tgcatgttga aggaactagg tcagtggtactatacaggac cttgttttac 10680 attctggatt tttcttttta ctttcctaat gatgtaatttaacttcttcc tgtattttcc 10740 atatttccta taaaatggta gttagatcta aaagcttgatttacttattt cagatttcta 10800 gtcaagggta ctcaatagat tgtattttct tttgcctcacacggaggtgc ataatgtctg 10860 cctggcctgt agtgatgcta aggttgatca ttctgttcaggtggcatcag tctgtgatag 10920 cttcctgtaa gaatcgttca ttaacctttc atctaatggttccattcatt catgatcttt 10980 aactgaatcc ctgttatttc attagggaat agcaaaataatgattttcta attctgttat 11040 tcctttcaca tttattaact gtaattcctc tgttaagagtagaactttta catattaata 11100 tcaactaggg ctgtatgttt accatgaaat ccaattcatgaaatacagga tgaatacttc 11160 aatcttttcc ttaattgcca cttgttatag caaggtattttggagactta ttatgataga 11220 tatgccacaa actgggtaac ttgaggggag tttaagtaaaggactgttta caaaggtata 11280 gataatattt aaggaaactg gtagggatgg tacagtgctctgtgactagt cacagtgagg 11340 aagggagttg cgatcgcctc aaggccagca ggaaacagggaagaggaaag cagctgagga 11400 gggtagctct atggaagagg tctgtctgat ggggagtgtgctttcaagta aagaggagct 11460 tgtggatcaa gtattaaggg agacagagga atatatcccctacccttatt ctcctccctc 11520 cctacatgca gtctccttct gagatctccc attacctgaaaagctaatat gcaaaagagc 11580 ctgttgatat gatccatatg agtcacaaac aagacataaccaacatttat ttatttattt 11640 cgctctcagt cttttttttt tttttaaatt tttggcatcattataaacac atttaaaaat 11700 atttttggtg tgttttcagt caatttatgt catccttgctgatactcagt ttgtcctgtc 11760 tttggctgat gggggcttct tcaggttgat tctcatgttatttgtatatg accctaatta 11820 tctttgatag agcttccttg tttttttttt tttgctgcagtaaggtactc aggctcatct 11880 tgtacatttc ttgcctcaga cttggaaatc agccattcctccaggaaact ctttcctttt 11940 ggtggggaat gctactcttt actattggat gaaattgtttcttgtccttg ttcagtgaat 12000 agagctatga tattttattt ttgagaagaa gaaaaagtgactgattattt caccaacttc 12060 ttgtcagtta ggctctttca ttttaggttt tttttttttttctttttttc cagtacttaa 12120 aaagtgcttt ctttactttt tgatgtaatt ttattttattgtgtatgtga aacatttaca 12180 tgctttttgg ttggtttggg tttttttgtt tttgttttgagacagtcttg ctctgtcgcc 12240 cagactggag tgcagtggca ccatcttggc tcactgtaacctcctccctc caggttcaag 12300 caattcttct gactcagtct ccctagtagc tgggactacaggcatgtgcc accatgcccg 12360 gctaattttt gtatctttag tagaggtagg gttttgccatgttggccagg ctggtctcga 12420 actcctgagc tcaagtgatc ttgcctgcct cagcctcccaaagtgttggg attacaggca 12480 tgagccactg cgcccggcct gttttttgag acagggtcttgctctgtccc ccaggctgga 12540 gtgcagtggc atgatcacgg ttcactgcag cctcttcctcctgggctcaa gcgattctct 12600 cacttcagtt tcctgagcgg ctgggactat aggcacgtgttaccatgcct ggctgatttt 12660 aaaaatgttt tgtagagatg gagtctcgct gtgttgcctaggccaggatc aaactcctag 12720 gctcaaggga tcctcccacc acaacccccc aaagtgttgggattaaaggt gtgagctact 12780 gtgccaggcc catttacgtg gtttaaaagt taaaactatatcagatatat tcagaatatt 12840 cttactttga tccacgtctc tctaccctgt tcttttccctttactctttt ttttgatttg 12900 acatttcagt gtttattctt ataaatataa gcaaatatgtatccatatac acatatacat 12960 agatatatac atagatatat ttatattctt ttttaatcactttcttacgc aaaaagtagc 13020 atgttacata ttggtgtatg attttcctcc ttcctttttacagctgagtc ctgttccatt 13080 ttgtgggtgt tagcattgtt aattcagtct cctatggatggacatttgag ttgtttatat 13140 ttttaccagt gtgtctttgg agtagattcc tagaagtgagattgctgggt taaagacaca 13200 tgcatatata attttgctca ctgttaaaat attctccctctcctaagagt tgtgctattt 13260 tacatttcca ctagtaatga tatgggattg tctgttgtcctcaccaacaa tatgttgtaa 13320 gacttttgaa tttttgtcca tttaataggt gacaagtgctatttcagtat aatttgaatt 13380 tgaattttat tttctatttt ttctattcta acattaatatcaatttctgt tcttctcatg 13440 agtaaggttg aatatctttt catattttaa gggctatttgaattccttct gtgatctgcc 13500 cgtccatatt ttttgttcat tttaaattgt ttttcttctcagtttttaga agctctttat 13560 gtattaggga gcttagccct ttttctgtgg taaaatgcagattttttttt ttttttgcaa 13620 tttttcatgt tttagacttt gcttctgcat tttgttactttgccgcacaa tttttaaatg 13680 tctccaaatt atcaatcttt tgttgcttca aaattttgagtcatagttag aaagatttcc 13740 cctatgtcca gatcataaag gaattcatcc atattttctctgatgcttaa tgtttttttc 13800 agtttttact ttgaaatcta ctcggaattt atcatgtatggtaaattttg agatacatcc 13860 aattttatct ttattcaaat ggctatctgt ttatctccacagcatttatt ttaaaaatgc 13920 ctatgtttgt tggttgacaa tgccactttc ttcatatattaaatttccat gtgtacttgg 13980 gattatttct aaccttctgt tccattggtc tctcttgctttttcatgtgc taataccaca 14040 ctgtgtcaat tataaaggct ttctaatatc tcttcagcttttaaaatgtc tagtagggcc 14100 atctccaccc ctgctctccc atgcacatac accgtcacattgaagcacat gggtgttttt 14160 tagggataac tagcttccaa ccctggcttt gttgtttactagcccttggt ccttggacag 14220 tttactaaag cactgtgata tccatttatc ccatctctgaaatgggatga tgatgcctac 14280 cttatattgt tgtaaggatt acatgacacc agcagaactgccttgtcagg catgaattct 14340 ggagtcagac tgggattttt cactcccacc caggtcgaccacttattagc cctgtgactt 14400 tgggtaaagt tcttaagagg aactcaaaca catcaccttcaaataagatt aatcatagac 14460 cctcatagaa ctcttaagag tattaaacgt ggtaatgcatgtaagtgctt tgcagagttc 14520 acaacattta gtagatgtta gctgtttgta atcatcgtcatcattatata tccttatgat 14580 ttgtccttgg gaaaaactac ttttgatcta agtggattattatctatctc ccagctctgc 14640 tggccaggtt tttatttagt tgtgtaatct tggacaagttacctaacttt tttgagtctg 14700 aatatattta atctgcaaaa tgagaatcat gataatacgtcataggctta attaggagga 14760 ttaaatgaaa taatttatag gtggtgccat ggttacatacaagtattagt agttaattct 14820 tttcctttgt ttacttttat agtataggtt ggatgaaggttccagtatag gcaaaaatac 14880 tacttggggg taaagtagag tgtgatactt tatttgaaatgttccctgaa tctgatcttt 14940 actttttgtt actgctgcac tacccaaatc caaattttcatcccaacatt cttggatttg 15000 tgggacagcg tagcagcttt ccaatataat ctatactacatcttttctta actttggtgc 15060 tttttgtcat gtggtctcag tgtacctatt tacttagtctttattcccag catttcccaa 15120 aatagactgc gctctactgg attttcttgc tttctatagtatctcaccta ctctttccta 15180 cttttgctgt cgtatttcca ggacctacct ttctctccccttgagttctc ccccatgtcc 15240 tcctcacaca attttctcat ctttatagtc tgacataaatcacaactgtg ttaaactgtt 15300 actttctttt ttgaactcct gtagtctcat gaaatttagaaattgatcat agttgtcttg 15360 tatagggcta ttttgtttgt taatcttacc taatgagaattccttgaggg ctaagcataa 15420 ttctagacat cagtgttcag tattacttat tgattggtgtgaatgattat ctcccctttc 15480 cccattcctt tgttttttcc ctttctttct ctgaattagttgatcatacc tatctggtat 15540 agggctatct tgtttgttaa tcatacctaa tgagaattccttgtgggcta aacataataa 15600 ttctagacat catgttcaat aatgctcatt aattattgtgaatgattatc tcccctattt 15660 ccattctctt gttctttccc tttctttctc tgaattagttaggactcttt cagttgtaag 15720 tgatagaacc caacataaat atttgtagac aggctgggcatggtggctca cgcctgtaat 15780 cccagcactt tgggaggctg aggcaggcag atcacctgaggtcaggagtt caagaccagc 15840 ctggccaatg tggtgaaacc ctgtctctac taaaaatacaaaagttagct gggcatagtg 15900 gcaggcgcct gtaatcccag ccactcggga ggctgagacaggagaattgc ttgaacccgg 15960 gggggcagag gttgcagtga gccaagattg tgccactgcacttcagcctg tgtgacagag 16020 tgagactcca tctcaaaaaa aaaaaaaatt cgtagacaaaaaataatttt aaaaatgctt 16080 tattttaaat ttttatggga acatagtaga tatatgtatttatggggtac atgtgatgtt 16140 ttgatatagg catacaatgt ataatagtca catcagggtaaatggagtat ccatcaccta 16200 caacacttgc catttctttg tgttaagaat gttttgattctactcttatt ttttttttta 16260 attttcaatt tttgtggata catagtaggt gtatatattatggggtacat gggatgtttt 16320 gatacaggca tgctgtgtgt aatcacatca tggaaaatgaggtatccatc ctctcaagca 16380 tttatccttt gtgttacaat ccaattattc tccattagttatttaaaaat atatgattaa 16440 attattattg actatagtta gcctgttgtg ctatcaaatactagatctta ttcatttttt 16500 ctagctagtt tttttttttt cttgtaccca ttaactatccccccagtccc tttctcccct 16560 actacccttc ctatctctgt ctccatgagt tgaattgttttgagttttag atcccacaaa 16620 tgaatgggaa catgtgagat ttatctttct gtgtctggcttatttcactt aatgtaatga 16680 ccttgagttc tatctatgtt gtgcaaatga taggattttattctttttta tggttgaata 16740 atatcccatt gtgtatatgt accgtatttt ctttgtccattgatcatgga cacttaggtt 16800 gcttccatat cttgggtgtt gtgaatagtg gtatgataaacatgggagtg cagatatctc 16860 ttcgctatac tgatttcttt tcttttgcat atgtacctagaagtgggatt gctggattct 16920 atgggtagtt tgattagttc tttgaagaac cgccaaactgttctccatag tggttgtact 16980 aatttacatt cccgttaaca ttgtaggaga gttcccgtttctccacatcc tcagttgtat 17040 tcattattgc ctcttggatt aaaaaaaaaa gctactttaactggggtgaa atgataccac 17100 attgtagttt tgatttgcat ttctctgatg atcagtggtgctgagtacct tttcatatac 17160 ttgtttgcca tttgtatgtc tttttttttt tttttttttttgagacagag tttccctctt 17220 gttgcccagg ctggagtgca atggcgcagt cttggctcaccgcaaccttt gcctcccagg 17280 ttcaagcgat tctcctgctt cagcttcccg agtagctgggattacagcca tgcaccacca 17340 cacctggcta atttttttgg tgtttttagt agagatggggtttcttcatg ttggtcaggc 17400 tggtctcgaa ctccagacct caggtgatct gcccaccttggcctcccaaa gtgctgggat 17460 tacaggtgtg agccaccgcg cctggccatc tttttttttttttgagacag gatctcattt 17520 tgttgcccag gctggagttc agtggtgggg tcatggcttactgcagcatt gacctctctg 17580 gctcagatga ttctctcacc tcagcctctt gagtagctgggactacaggt gcgtgccacc 17640 atgcccggcc aatgtttgta tttttttttc tttttttgtagagacagggt tttgccatgt 17700 tgcccaggct ggtctcaaac tcctcggctt aatgatcctcctcccaaagt gctgggatta 17760 taggcatgat agacgtcttc tttggagaaa tgtctattcagatcctttgc ccatttaaaa 17820 attggattat tagacttttt ttctcctgag ttgtttgagctccttatata ttctagttat 17880 taatcccttg tttgcacata ttttctccca ttctttaggttgcctcctca ctttgttgat 17940 tctttaaaaa agaattttgg attcaggaaa acctagtctcattaaaggta aagattaagt 18000 tgaacttcag taaaaactag aatttgggct taaatgcaagcaaggctcat tctctgtgtt 18060 ctcttacgtt tttctttgtg ttggcatcat tctctcagattgtagactgg ttttagtcac 18120 atgtggaaaa tatggctgcc tacagcactg gaactttatatctaacagct tcagctttga 18180 ccattgactt cagatctttg agtcttgagt atagagctctggaggaaaag attcattagt 18240 tacatttagg tcagttgcca ggtgcccact ccttgactaatttttttttt ttttttgaga 18300 ttgagtcttg ctctgtcacc caagctggag tgcagtggcatgatctcggc tcactgcaac 18360 ctccacctcc caggttcaag cgatcctcct gcctcagcctcccgagtagc tgggactaca 18420 ggtgcacacc accacacctg gctaattttt tgtatttttagtagagatgg ggtttcacca 18480 tattagccag gatggtttca atctcctaac caagtcttgatctgcctgcc tcggcctccc 18540 agagtgctgg gattacagat gtaagccatc gtgcctggctccttgactaa ttaattgtga 18600 tcaggcgggt agggtcacat gagaactttg agaatggggaggcagtttgc aggaaaatga 18660 gggcagtagg gcagggcaga cagtgccgta ggtgtacattatgctaccct ttctttcctc 18720 tttgtcctta gagaagtcat atacactctt ctaaagtgcagttttctcaa tctgtaaaat 18780 tgaattaaca ataatttatt tcatggggta ctgaggaggactaaatgaga taatttagaa 18840 agcagcattt gaaacttcac aaaaacaata gagtaacctggtgataagtg gctgtacctt 18900 ggtctctcac aacgtgccct gtgcttattt ggcctggccccaaaggtttt gcaaatgatg 18960 tgactgcagc tggttttcca gatcggcttc tttggtgttgtccaccagag aacttgtgat 19020 tacaagacga gtgccagtag cttctaagac tatttgttcaggtacagtgg aaaaagtctt 19080 ttcttcttac cttcaaatag aatctctaat tgatctgaccaatttagata taatgtaccc 19140 atatctgaat caatctcagt caaaataatg gtgtgctgatgagcataggt ctgcatcatg 19200 tgctccagtc ttggaactgg ggaagagcca gcttataccaagatacatta cggttgcatg 19260 aaggaggggt ggaaattgga gttcatttac cattattaaattatctgaca ttaaaataaa 19320 tattgccagc cgtttgccaa acctcaactt aagaccaccaactgagatac acttggacct 19380 ttaagagagg gatacctgtg tgctgcattg ccttgatgatatgtcatagt gaggattacc 19440 aaatgcctgt tgatggatcc agtcacatta gaaccaaatacagattcttg tgattcatca 19500 cagctccacc atatgagaat tgaagaaaaa gtcttgtttcccatttaata aattatttta 19560 tcttttaaaa aatgtggttt aaaaaatgcc aggctatatgaagaggatga gcattttctg 19620 acacttgcta aaaagaagtt gtgttggttt tttttttttttttttttttt taaagatgag 19680 gtcttgctta cccaggctgg agtgcagtgg tgcgatcatggctcactgca gccttgacct 19740 cccaggctga agcagtcctc ccacctcagc ctcccgagtagctgggacta cagacctgtg 19800 ccatcacagc tggctaactt tgttgcattt tagtacagatgaagtcttaa cgatgttgcc 19860 cagcctggtc ttgaactcct gggctcaagc aatcctcccaccttggcttt ccaaagtgct 19920 gggattacaa gcagtgcctg gccggttttg ttttattttgtttggttgat ccaatttttt 19980 tagtggggaa gggaaaatca aattttatac agaagccaaataaagagatt cccttaatca 20040 tcattgtaac tctccaatac cttgttcctt cacctgtcaaaaggaaggga ttggctgttt 20100 agtaatttat gaagacatta ttttaatgtg aagaggctataaaataatag ttttacaatt 20160 ggtccctgaa cagcatggtg gttagaggtg ctgactcccctaataattga aaatctgcat 20220 gtaactttga ctcctcccca aatttaacag ttttttattttatttatttt ttgagacagc 20280 gtctcgctct gttgcccagg ctggagtgca gtggcatgatctcggctcac tgcaaccttt 20340 gccccaccgc ccacccccgg ttcaactgat ccttgggcttcagtcttcag agtagctggg 20400 attataggca tacgacacca cgctgggcta atttttgtgtttgtagtaga gacgggtttc 20460 accacgtagg ccagcctggt ctcaaactca tgggctcaagtgatccactc gcctcggcct 20520 cccaaagtac tgggattaca ggtgtgagcc actctgcccagccccagatt taacaattaa 20580 cagccttact aatgtaaaga ttcaactagc acatattttgtgatatatgt acatagttat 20640 ttaatttttg agatggaatc tcgctctgtc acccaggctggagtgcagtg gcatgatctt 20700 gactcactgc aacctctgcc tcctgggttc aagtgattctcctgtctctg cctcccgagt 20760 agctgggact acaggtgcgt gccaccacac ctagctcatttttgtatttt tagtagagac 20820 ggggtttcac catgttggcc aggctggtct tgaactcctgacctcgtgat ccacccacct 20880 cggcctccca gagtgctggg attacaggca tgagccaccattcctggcta ttatatatct 20940 tatatactgt attcttacag taaagtaagc tagagaaaagaaaatattag gaaaatcata 21000 agggagagaa aatatattta ctattcatta agtggaggtagatctcataa aggtcttcat 21060 cctcgttatc ttcatgttga gtagctgagg aggaggagagattggtcttt gtatcagggg 21120 tggcagagag ggaagaggta aaagagatgg aaggggaggcaagagaggca tactcggtgt 21180 atattttatt gaaaaaaaaa atccacatat aagtagatctatgcagttcc aactcatgtt 21240 gtttaagggt caactgttac agaaaaacag atattttcacagattaaaaa ggtttaaaaa 21300 tttactgttc ctatgagatt aatttttttt ccctaaatggtaataaattt tgcttactta 21360 gcttttatgc ttctgatcta ttgtactttg agatactgatgctcttacat gtaagctctg 21420 tgagaacagg tatctcattg tcttgttcac tgttgtatcactggttctta gagccctgct 21480 tggcacatta taagttttca ataaacattt atttgttgttgtcgaatttc tggaaaatat 21540 cttttgctta tttgtttctg taacaagagc tgaagatggttgacatcatg ttagggaata 21600 ggatataatt gataatttgt ttaaatgtaa actgtacctatccgggggat aacattttaa 21660 aactgcagga ggtctgtatg aaaatataga tattctgctccccaccccag tacctcttga 21720 attttgaatg aaggaccaaa agccaataat gtgttgttttgtgtgtgtgt tttttgatac 21780 agagtctcaa aaataaaggg aatcacttta tttggcttctgtatgcagtg gtgggatcat 21840 gattcactac agcctcaacc tcctgggctt aagtgattctcccacctcag cctcctgagt 21900 agctgagatt acaggcatgc taattttacc tggctagtttttgaatgttt aatagagaca 21960 gggtcacact gtgttgccca ggctggtctt aaactattaggttcgaggga tcctcccgcc 22020 ttggcctccc aaagtgttgg ggttacagat gtgatgaaatgtgtcaacat ttggctgcat 22080 aattcaagga accagtattt tctaagtgac caatcaatgcatgatgttat aaaatcatcc 22140 atgaataaaa gatccattca aagtagaaga tagaccaataaattttaatg ttgcaaactg 22200 tgcaaaaagt tcagaaacca ttgatataat ttcagattctacaatgccac taatctttaa 22260 gaaacacccc tcgtcaaatt tgatgtagta gcaaataatttccagttatc tgaaaagact 22320 gttaacatac tcttcccttt tccaactaca atctcagacttaataatggt tcactttatg 22380 atttttccac tttatgatgg tgtgtgtaaa gctatatgcattcagtagaa accgtacttt 22440 gagtacccat acaaccgttc tgtttttcac tttcagtacagtattcaata aattacatga 22500 gatatttgac actttgttat aaaataggct ttgtgttagatgattttgca caactgtagg 22560 ctgatgtaag tgttctgaac atgtttaagg taggctacactaatctatga tgttagcaga 22620 ttagatgtat taaatgcatt ttcaacctac aatattttcaacttacaagt ggtttatcag 22680 gatgtaaacc ccacccatta taagtctagg agcatctgtccatatctgtg tgaagttaga 22740 ttttcacgct tcaaaccaag gcaatgtatt gtcatagattgaatgcaggc gcagatatga 22800 gtgtccagtt gtcttctatt aagctagatg ctaaagagatttgtaaaaat gtaagataat 22860 gccattcttc ttcccaatta aaaaaagttt taggccggctgcggtggctc acgcctgtaa 22920 tcccagcatt ttgggaggcc aaggcgggtg gattgcctgaggccaggagt tcaagaccag 22980 tctggccaac atggtgaaac cccgtctcta ctaaagatagaaaaaaatta gccgaatgtg 23040 gtggcgtgcc cctgtaatcc cagctacttg gaggctgaggcaggggaatt tcttgaacca 23100 ggggaacttc ttgaaccagt ggaggttgca gtgagctgagattgcgccgc tgcactctag 23160 tctgggcaac agagcaagac tccgtcttaa aaaaaaaaagttttaataaa atatgttatt 23220 tataataaca tataatggga atttttttta aattcctgtcctagtttatt tctgctgcta 23280 taacagaacc aagactgggt aatttataaa gaacggaaatttgttttctc atagttctgg 23340 agtctgggaa gaccaagatc aaggcaccag caggtttggttctctgatga gggtctagtc 23400 tctgaatcca agaaggtgcc tggaaagctg catcctccagaagggagggg tcagtatgtc 23460 ctcacatggt agaaggcaaa aggacaaaaa gggtccaaactccctaatca aacctgttca 23520 tcatggcatt aatatactta taaggactct gccctcatgacctttacacc ttcccaaagg 23580 ccccacctct taacactgtt tctctgggga ttaagtttccaataaatgaa ttttggcact 23640 ttcagaccat agcaaatttg ttcagatgat ccctttctagtttcttgaga aaggaactta 23700 gattattaat tgaaaattat tattcttttt cttttcttaaaaaatttatt ttaagacagg 23760 gtctcactct gtcacccaga ctggagtgca gtgacgtgatcatagctcac tctagccttg 23820 acctttcagg ggctcaggct atccttctgc ctcagcctctcctgagtagc tgggaccaca 23880 ggcgtgtgcc tcgatgcccc gctaattttt aaatttttttgtagagacga ggtctttcta 23940 ttgttaccca gatgggcctt gaactcctga gctcaagtgatccttccacc tcagcctccc 24000 aaagtattag gattattggc ctgagccact gcgcctggccatcattactc ttttctaatc 24060 atttaatgct atacattttt cttttagcag tgatttagctgtattccaca aattccagtg 24120 ttgtgttctc attttcattc agttcaatgt atatttttatttcctttgag gctttccctt 24180 tgacccagaa attatttaga aatctgttgt ttagtttccaagtgtttgga gatttcccta 24240 ctatcttttt cttatttatt gtagtttgta cattttggtcaaagaacaca ttctgttatg 24300 attttaattc tttttaattt gctgaggttt gaattatggcacacaatgtg gtggtctgtc 24360 ttggtaatag ttccatgagc acttgtaaag gatgtgtattctgctatttt gggttagaat 24420 gtgatataaa tgtcaattag atcttgttga ttaattgtgttgagttcttg atgattttgt 24480 gcccagttct atcaattttt aagagagaag tattcaagttgccgactaaa attgtagatt 24540 tgtctatttc tcttttcatt tttgtcaggt tttgcttcacttgtgttgca gttctgttgt 24600 tctgagcgta catggttcag attattgtgc cttcttggtggattgactct tttgtgatca 24660 tataattttc cattctgtgc ctggccattt tctttctctgaagtctgcct tgtctgacac 24720 taatatagcc acacccactt tcttttgatt aatatttgcatggtaaatca tttcccattc 24780 ttttacttcc aacctacctg tattgttgca ttttaagtgaatttcttgtc tgtggatggg 24840 tcatgtttat gtatccaggt ctaccaatct gtttcttaatttgaattgtt gtctagacca 24900 tttatatttc atataactat tgttatgtta gggtttaaatctgctgttat attctttctt 24960 ttctgttttt acctccctgt tttttgtatc tgtgttccttttttgccttc ctgggccact 25020 tgaacattta ttgtaattcc cttttgactt atctgaaagtgtttttgtgt gtatatgtct 25080 ttgtatagct ttttaaattg gttactctag gtattaaattaggcatagaa aacatcacat 25140 tatattggtt ttgatatttt actagttcaa ttgaaaggtagaaatttttc ctcctttttg 25200 tcattttacc cttgttcgta atatattcat gtttaatatcttcttacatg cattgagaaa 25260 caagcaacat tataattttt gctttaactt tcaagacaagaaaaatgtat tatcttttcc 25320 catattttta cattttgttc ttcttttctg atatatgatataccagattt atttctttca 25380 tcgtcttcta tttagagaat ttcctttaac cattattttagaatgtctgc ttgtgacaaa 25440 tctcttggtt tttcatcatc tgagaatgtc ttaatttttttttttttttt cagtggatgt 25500 agaatttcgg tttgacattt taaaaaattg gcttaggtttctttgggtgt gtcctgtttt 25560 gatgtttggt cagctccttc aacctgtagg tttatgtcttttgccaaatt tggggaattt 25620 ttagccatca tttgttttag tcccttttag ccccattctatttttcttgt ttgtcttctg 25680 ggcatccagt gatacaaatg ttaaatcttt tgttatagttccatggaaaa tgaatgattc 25740 tcttcatttt cttttccatg atcaaggtct gattgtatgataatttgtat aagaatttta 25800 agaaaataac aattagctga gttttaaaaa attattttcaggctgggcgc ggtggctcac 25860 gcctgtaatc tcagcacttt gggatgccta ggtgggcggatcacttgagg tcgggagttc 25920 gagaccagcc tgtcccacat ggtgaaatcc tgtctctactaaaaatacaa aaattagcca 25980 ggtgtggtgg tgcatgcctg taatcccagc tactcaggaggctgaggcag gagaatagct 26040 tgaacctggg aggtggaggt tgcagtgagc cgagattgcgccactgcact ccagcctggg 26100 cgacagagtg agactcagtc tcaaaaaaaa aaaaaaaaaattatttccat actactacta 26160 accaaagaat gtatgacatg tatttatttt tccccaaaataacaaattag atacttaaaa 26220 gaatcgtttg agaaatgaca aactctctct ccccaaagtctgaaccatct gaacttcttc 26280 agttactcat gccaggagaa aactttgatt gtgttcacccatacctataa aactttaagg 26340 cagtagggag gaaatcctcc ttgaaattat ccgtgaatatatttttgtga ctcatttgga 26400 taaatcttta aaaaataaaa aacagtcctg tgtggttgttactgatacta tcttgtttta 26460 cagatgagaa cattgaggtg gtgatgggtt aaataacttgctcaagatca catgactact 26520 aaatattcta gcttggaata tgagacagga tgccatttctgtagaagtat tctcttactt 26580 tacaaggtta atgtcttaac ttggtatctt aagcctgttctcccttacat cttttggaac 26640 cttgttaaat catgtatctt ctataaaaat atcttttctacctaaaaatg tttcatcaag 26700 tttaagtttt aaatcatgca aacttctagt ctttctcatttatttagcca tttttctcat 26760 ttcttcattt tccattaatt tttctaccaa ccataagctggtatgtgcac cttctcctct 26820 aacgaagcat tctaattaag attaataatg cccttaaaaattggcagtaa aatatacgta 26880 acatgaaatt tgcctttttt ttttttttga aatggggttgtccaagctgc tctgaaattc 26940 ctgggctcag gcgatcctcc cacttcactt tcctgagtagctgggattac aggtgcatgc 27000 cactgcatcc gcctccgttt taactgttcc tgagtacaatttggtggtgt taaagcacat 27060 tcacaatttt gtgttttcac cagtatctgt ttgcactatatcatctgtct caaacaaact 27120 ctgtatccat taaacagtaa ctcttttatt ccctgtccctccagcctatg gtaatctcca 27180 ttctactttc tgtttctgta aatttgccat ttctaggtactgtgtataag tggaatcata 27240 caatattttt ccttttttgt ctggcttatt tcatttagcataatgtttac agattttatc 27300 tatgttgtag tatgtgtcag catttccttt tttaaggctgaataatacta cagtgtatgt 27360 acataccacc ttttatccat tcatctgttg gtgtacaattgtgttgtttc taccttgagc 27420 tattttgaat atgctgctgt gaacattggt gtacaaatatctgttcgaat cccttctttc 27480 ggttcctttg ggtgtatacc cagaagtgga attactgatcaggtggtaat tttatgagaa 27540 actgatacac tatttttcac agcagctaca ctgttttacattcccaccag cattgccaag 27600 ggttccagtt tcattacgtt tcattccagt ttcattctaacacttgttct tttctgattt 27660 tttaaaataa taactatcct aatgggtgtc aagtgatgtttcattgtggt tttgatttgt 27720 atttccctaa tgatttatga tgttgagcat cttttcatgtgcttattggc catttctgtc 27780 tttggagacg tttttattca agtttttgcc cattttaaaattgagttgtt ttgaatttta 27840 ggagtgccct acatattttg cgtaataatc tcttatcagatatattattt gcaaatattt 27900 tctcccattc tgtggattgc catttcactc tgttaatactgttctttgat gcacaaaagt 27960 ttttaatttt gatgaggttt agtttgtttt tttttttgttgcctgtgttc ttggtctcat 28020 gtacaagaga tcatggtcaa attcaatgtc ctgaagcttccccctatgtt ttcttctaag 28080 cattttatag ttctagcttt tatgttttgg tctttgatcccttttgagtt aattttggta 28140 tatggtatat tgtaggggct cccacttgat tctcttgcatgtggatatgc agtttttcca 28200 gcaccatttg ctaaaaagac tgtccattat tgaatggttatgtcacacat gttgaaatta 28260 attgaccata tgtatgtgtg ggtttgtgcc ccttttccccccgccattcc atttttctgc 28320 acaattgacc cttgaacaac atggggatta ggggcactgaccactaccct ctctccatac 28380 atttgaaata catgaaatac atactttttt aatttccccaaaacttaact actaatagcc 28440 tgctgttgac tgaaagcctt actgatagca aaaccagttgattaacaaca cgtattttgt 28500 atgttatatg taaatatatg tactgtattc ttacataatgttagccagca aaaagaaaat 28560 attactaaaa tcataaggaa gagaaaatat atttactgttcattaaatgg aagtggatca 28620 tcataaaggt cttcatattc accatcttca cattgagtaggctgagtaga aggaaaagga 28680 ggagttggtc ttgctgtttc atgggtagca gaggtggaaggaggtataag tggactcatg 28740 cagttgaaac ttatgttgtt tgagcaccag ctgtatgtgcttgtccttat gccagcagta 28800 caccattttg gttactgtag ctttgtagtg atttttgaagtcagtgaatt tgagtcctct 28860 aactttgttc tttttcaaga ttgttttggc tatttgtgatctgagattac atttgaattt 28920 taggatgagg ttttctattt ctgcaaaaaa caccattgggattttgatag ggattacatt 28980 taatctgtat attactttga gtagtattgc cattaaaaaaaatatttttt ccagtccatg 29040 aacataggat gtcttttcat ttatgtcttc tataatttcagcaatgtttt atggttttca 29100 gtgtacatgt ctttagcctc agttaaattt attcttcagtattatttgtt ttgatgcttt 29160 tataaatgaa actgctctta agtttctgtt tggattattcactgttagta tatagaaatg 29220 caactgattt ttgagtattg actaacctgt aacttggctgaatttggtta tgggctctga 29280 cggatttttt tgttttgttt tgttttggtg tgtgtgtaatctttagtttt ccacctatag 29340 gatcatgtca cctgtgaata gataatttta tgtctttgtttccaatttgg atgcctttta 29400 tttctttttc ttacctaatt gctctggcaa aaacttccaataatgagttg aatagaagtg 29460 acaaaaggtg acatctttgt cttgttcctg atctcaggagaaaagctttc agtctttcat 29520 cattgagtat gatgttagct gtgggatttt catatgtggccttcaccatg ctgaggaagt 29580 ttctttccat tccctgtttg ttgattgttt tttattatgagagggtgttg aattttgttg 29640 aacattttat ctgtatctaa tgaggtgatg tggtttttgtcctttattct attaatgtgg 29700 tagattacat taaaatattt ttgtatgtta aatcaactgtgcattcctgg cataaatccc 29760 acttgatcct ggtgtgtact tcttctcata tgtgctatattctacttgct gttattttat 29820 tgggcatttt tatgtctata ttcatatgtt tcataaactctgatatgtaa tttttgtgtg 29880 atatttttag ttttgctatc agggcagtgc tgcactcatagaatgagtta ggaaatattt 29940 ccttctcttc agttttttga aagagtttga aaaggattcgtgttaattct tctttaaacg 30000 tttggtagag gccggacata ctgcctactc acaatctgtaatcccagcac tttgggaggc 30060 caaggcaaac aaataacgtt gagctcagga gttcaagaccagcctgggca gcatggtaaa 30120 accccatccc taaaaaaaaa aattaaccag gtgtggttgctcacacaagc tactcaggag 30180 gctgaggctg gaggatagct tgaagtccag gaagcagaggttgcagtgaa ctgagactgt 30240 gcccctacac ttcaccctgg gcaacagagt gagaccctgcctcaaaaaaa ggaaaaaaca 30300 aacaaataaa aatacataaa tgtttgttag aattcaccagtggagccatc tggcccaggg 30360 attttctttc ttgagaggtt tattattacc atttctgtcttcttacttgt tacagttatt 30420 ggtgtattct gactttccat ttctttatgg ttcatttttggcagattgta tgcttctagg 30480 aatttgtcca ttttatgtaa gttacccttt ttttctggtgtgcagatgtt cataatattc 30540 tattacaatt atttgtagaa ttgataggaa tgtcgtcacttttatttctg aaattactaa 30600 tttgagtctt ctctcttttt cccttagtca gcttaaccaaatatgtgcca attttatctt 30660 ttcagaagac ctactttggg tcacattggc tttctctgttattttcctat tctgtgtttt 30720 atttatcttc actttaatct ttattctttc cttctgtcagctttgggttt catttgcttt 30780 tctttttcta gttccttata gtattaagtt aggttatttttattgatttt aaaagctttt 30840 tttcttttta aaatgtaagc atttatagct ataaatttcctcctttgcac tgatttttca 30900 gcatcctact aggtttggta cattgtattt tcattttcatttgtctcaaa gtattttcta 30960 atttcccaat gattttttct ttgacccata gttttaagagttcattgtct aatttccacg 31020 tttgtaattt tccagttttc ccctattatt gtttagtagtttcattccat tgtgattgga 31080 aaagatactt tgtgtcattt tggtctttta aaagttattaaaacttgttt tgtggcataa 31140 cataataagt ctattcttga gaatgttcca tgtgtacttgagaaannnnn nnnnnnnnnn 31200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 31260 nnnnnnnnnn nnnnnnnnnn nnnnngttat aggtcgtcatgtctctgttt tttaataatc 31320 ttctgtctgg tctattcata ctgaaaatgg attattaaatctccaactat ttgtagaact 31380 atgtattctt tcttaaatct ttcaatgttt gcttcacatatcatggggct tgtatattta 31440 tataccaaat gtacatattt gacgcatata aatttatgataatatatttt tggtgaatta 31500 actcttttat caatatgtaa tgtctttttt gtctgttaaccattttttta acttaaggtc 31560 tgttttgtct gatattagcc tacccacctc agtgctttttttatttacta tttgcatgga 31620 gtattttttc ccatctttca cttttaacca tttgtctttggacttgaagt gagttcctta 31680 tgggcagcat atagttggat catggtgttt ttgtttgttttttaaaatcc attttgtcag 31740 tctctgtagt ttgattggag agtttagtcc atttatacttaaagtgttag tttttagctc 31800 cagaatttgt ttggtttctt aatatagttt ctttctctttgttgacattc tcattttggt 31860 catatataat tttcctagtt tcctttagtt ccttttctgtgttttccttc aactctttga 31920 acatatttaa gacagttttt aatgtcttta tcaagtccaatgtctgggct tcttggatat 31980 ggtttctgtt catttatttc attcctccca gccgtgtttttctgttcctt tgtatatctt 32040 gtggtttctt tatggaaaat tggccgttta actgttataatgtggtgatt ttggaaatta 32100 gattttctct attctccagg gtttgctagt gatatggctttgatgagtgg gggaacacca 32160 gggtcttcgt ctcgagtcga attagaaaaa aactacaggaacacacgtgg atcacatacc 32220 gaaaggaagg agaacgcttt tgttctcact tctctttctagatgggtaac agatcgtctt 32280 caacatgcac tcccctggag tgtattttaa agcactgggactccttcgac cctaaaactt 32340 tgaagaaaaa gcagtattct actgcacaag ggcatggccttcctatcctc ttcgtaaacc 32400 tggcctgggg agacttaatt ttaatattat ccaacagttagaacttttct gcacacagga 32460 gggcaaatgg actgaggtcc cctatgtaca ggctttctttgctctgcgag acaacccaga 32520 cctttacaag ttctgtacaa tcgacccagt tcttttagtagccatggcag gcaaacccac 32580 agggagtagt tccccagagc taaagcgggc tccagaggagcaatctaaga cagctactga 32640 agtcccaacc cttccggttc cactccagtt gtaccatcagcaccaccatc tccagtatgt 32700 cctactctcc cctcttcact cttacccctc caggaaatgcctgatgggaa tggtgccata 32760 agggttcaag ttcccttctc attgcaggac gttaagcaaacaaagggaga cttaggtcaa 32820 ttttctgatg accctgatag gtatatagaa attttctgaaatttaactca ggtgtttgat 32880 ctcacgtgga gggatgttat gttgctgcta agttaaaccctcactacagc tgaaaagcag 32940 acagttccgc aggcagcaga aaaatatgga ggtgagcaacatgcctccat acagcagacc 33000 aaggaaaaaa agaggagaaa aggaaggtga ggaagaggtgaaaacttcac tcctgcaagg 33060 aagggaagca gttccggtaa ataaccctaa ctgggatcccaataactcag cagatgaatg 33120 gaaaaggaag cactttttaa ggtgtatatt agaaggcctgcgaaagacca cagctaaacc 33180 tctcaattac tccaaactgt ccatgataga ccaaaaaccagacaagaatc ccacagcttt 33240 tatggaaagg ctgagagggg cactaataaa gcacacttctctatacccca atttagttga 33300 gggacagctc atcctgaagg acaagtttat tacacaggcagctcccaata ttagaagaaa 33360 gctacagaag caagcagtag aaccagatag caccctagaaagcctcctga gaatagccac 33420 ttcagtcttt tacaaaaggg attgggagga ggcccgagaaaaggagagga agtacaagag 33480 aaaaacagag gctctagtag cagcattaca agcttgtaaagtcctgaatc cccaaggtgc 33540 atccactagc tgttatcaat gtggtcagtc agggcattttaagaaggaat gcccaggaag 33600 caagacaaag ccaccttgac cctgtccagc ctgtggtggagactactgga gacagacctg 33660 ctttggtctt ttgttctggg ttcagaacta gtctcacagatggtccagca ggactgatgg 33720 gtcccagagc tcaaactcca tccactcaga ctgccattacagcacaggtg ccccgggctt 33780 tcctgctaga caagagctgc gaaaaacctt ccaaaggacccttttttttc ctctctatct 33840 gcctaaaata atttcttaat aactcctacc acactaggttctctctctct ctctctctct 33900 ctctctgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtaggtgggtgttt tgagatagag 33960 tcttgctctg tcacccaggc tggagtgcag tggtgccatctcagctcatt gcagcccctg 34020 cctcccgggt tcaaacgatt ctcgcaccct agccccccaaacggttggga ccacaggcgc 34080 acgccaccac acccggctaa tttttgtatt tttagtagagacggggtttc gccatattgg 34140 ccaggctggt ctcgaactcc tgggctcaaa tgatctgcctgccttggcct cccaaagtgc 34200 tgggattaca ggcatgagcc actgtgcctg gccagtttgttttttattgc tgatggctgt 34260 agtacaaatg actgcagtcc atttgttttg agacttttccaaattgtttt tgcaaagact 34320 attccttact gtgagtgatt actgaagtct tggttcctttagcttgtatt cagctaatgt 34380 ttggacagag atttccttga atgtgagatg ctccaatacataccaaaaaa gcctcagaaa 34440 acaaaaagag aaccacctct cagtctttgc agatttgccctctgctgggg tactccttta 34500 ccacttagct aggcttgttc tgagtttcag gatgaaactcacatgagatg agagcttagg 34560 atcttctcag gattttcctt agcatgcttt ttttgcctgggcttcacacg gctttttata 34620 ttctcctgta tacatggctg cttttgaatc atctaatttcccaaaaaagt ctcgccccac 34680 ttctcctctg ggcctagatg gtctattgta tgtgttgtgtccattagtcc tccctactcc 34740 cccatctttt acctcaggtg tgtcagtagg ttttgtagttagcttccttg tagcctttat 34800 gagcagcacc agctgctttt cctgcctgtg ttctgaattatgtgaaacag agtttaggtt 34860 cttgccttag tccttcaggt atctcccata caagatagaatgaacataca cagtatggtc 34920 ttattctacc ctcttgggtt caaggtgtgg gggagtaggaagaggaggaa ccagtctgct 34980 acctgctcaa gacttaagac tgtccttggc cgggcgcggtggctcacgcc tgtaatccca 35040 gcactttggg aggccgaggc gggtggatca tgaggtcaggagatcgagac catcctggct 35100 aacaaggtga aaccccgtct ctactaaaaa tacaaaaaattagccgggcg cggtggtggg 35160 cgcctgtagt cccagctact cgggaggctg aggcaggagaatggcgtgaa cccgggaagc 35220 ggagcttgca gtgagccgag attgcgccac tgcagtccgcagtccggcct gggcgacaga 35280 gcgagactcc gtctcaaaaa aaaaaaaaaa aaaaaaaagactgtcctttt ctctggcagg 35340 gggtgggaca agagcaagta aactgccata acactttcctaccatttggg atagctttta 35400 cttgattgga tatttgcttg ttttttgcta taaacctaaattttccagaa ctattgcaga 35460 cctgcttcag atagcttgtt acttttgatg tttctgtcggtgaaggagag cttggagctt 35520 cctagtccac caatttgctg acatctgata gtgacttaaaataaattatg gctctgtgtg 35580 tgtgtgtgtg tgtgtgtgtg tgtgtgtatg taccaagaggggaggatcac ttgaggtcag 35640 aaattttagt ccaacctggc caacatggtg aaaccccatctctactacaa atacaaaaat 35700 ttttatgtgt gtatatatat gtacacacac acaaacgtatcataaaactt atcatttaac 35760 tgtttctttt ttgtttgaga cagactctcc ctctgtcacctaggctggag tgcagtggca 35820 cgatctcggc tcactgcaat ctccacctcc tgggttcaagtgattctcct gcctcagcct 35880 tctgaatagc tgggattaca ggtgcctgcc accatgcccagctaattctt gtatttttag 35940 tagagacggg gtttcaccgt attagccagg ctggtcttgaactcctgacc tcaagtgatc 36000 ctcctgcctt ggcctcccaa agtgctggga ttacaggcatgagccaccta gcccatttaa 36060 ctgattctaa atgtatagtt ctgtggcatt aatcattcacgttgttgtgc gaccatcagt 36120 gatgtttaaa tggcaaatga tttggcttct tttgcattctctattctgtt tgacttcctg 36180 agatgtttta aattcattct taaggcattt cttctttttaagttccttct cagcttcctt 36240 ggtactattc tgtctttctt ctctgtgtcc tcctccaccatttttgctct gtgtacctct 36300 tttttttctt tccttttttt ttttttttga gacagagtcttgctctgtca ccaggctgga 36360 gtgcagtggt gctatgtcgg ctcactgcaa cctccgcctcccgggttcaa gcgattctcc 36420 tgtctcagcc tcctgagtag ctgggactac aggtgcgcatccccataccc ggctaatttt 36480 ttgtatttta gtaaagaagg ggtttctcca tgttggtcaggctggtctca aactccgacc 36540 tcaggtgatc caccagcctc ggcctcccaa agtactgggatttcaggtgt gagccaccat 36600 gcccagcctg ctctgtgtac ctgttaaatg ttagtgtttcccagtgttct attcttcagt 36660 ctttctctac tctccctgtt tattcttgtt ggatgattgcatatgttctc atggatttaa 36720 gtgtgctctc atgactccca aatctgtatt gtagtcccaatctctctgtt aaacccaaac 36780 ttatattcag ctgccatcta gttattcatg aaatgatatcccaaaggacc tcagatgtag 36840 tgtgtctgaa atatttatca tctctccaaa ttatattatctgccttggtt aatgaagtca 36900 ctgtctactc acatcaactt gaagatcata taatcattttcaacccttcc tgttcctctt 36960 tcaattattc atcagattct tttgcctcta atgccccattcctgtttacc cagtttttca 37020 gtcttttatc cttttatgct tgtattacca cagtatcctcctagttgctt tcatctccac 37080 ctgtctcttc tacttttaat ttatcatctt tatcattgccagactactaa aataaaaacc 37140 agttatgcca ctttctcccc cgcagaaatt cctgtggttccaaattatca gaaggtaaat 37200 tcttctcctg gtatgttata cctttaacat tctctccctgatcatcacag ctttgactcc 37260 ttctccttca tctccttctc acctttccca gtccagaacattctatcctc attctctgaa 37320 ttctcgttgc ttttcacctc tagattgcct tgccccacttctgtcttatt ccttcataat 37380 tctattcttc aaggttcagc tcaaatttca tcttctctgtgaagccccaa atagcctttt 37440 ctctatttat gtagtaccta cttataacac ttgatatcctgtatttactt tttctaccta 37500 ctaactaact tctgaactct gagagacagg aagtattttttttctgcttt cctgcagaag 37560 aggaggcaga tttagaaata aataattaca gtataacagctaaatacata atttctgtaa 37620 tagcacaaag gaggttatgt aacatttccc ctcagggcactggggaaggc agcacaaagt 37680 aagggacatt tgaggcagtc ttaatgtaag aatgttcacattccagcggg agaatagagc 37740 aggtgcaaag gccccatagg gtagagtgag cctggcttatttgggaaatg gctggtagca 37800 cactgtggcc taagcccagc tatgtatgtg tagagaaacaggctggacaa aggtatgatt 37860 ggagactgat tgggaagagc cattgtttgt tgtactagataatttggaac attatttact 37920 tatttattga ctataaatgt cctgggcagc attgacatgggtatagtaat gtcacaccag 37980 ttaaacagat tttaactgta tttaatatca ggttgttaaaattccctaag gaaattctgt 38040 tttcattttc atagctgtgc ttaattttga tgaattatttctttatcaag tgaagagaga 38100 ttgacagact gattgatttt tagagacagg gtcccactctgtcaccaggg ctgtagtgca 38160 gtggcaaaat catagattac tgtagccttg agctcctgggctcaagcgat cttcctgcct 38220 cagcctctca agtagctggg actgcaagca tgcggcaccacgcccagcta ttttttttac 38280 ttttattttt gtagagatag ggtcttgcta tgttgtctaggctggtcttg aactcctggt 38340 ttcaagtgat cctcctgtct cgatctccca aagtgctgggattgcagttg tgagccacag 38400 catctggcct aagggactta gttaaaacaa cattctactacatataccaa aaacattttc 38460 tttaaatggt atatcctctc ttaccaaagt tggtcagcagtctttagtac cagtggtcag 38520 cccattttag aagtagtttg attttcagaa gccccagttttgctgccata gttttcaatg 38580 gattttgagg gtggggtggt gtttctccaa aaatatgtctgttaggttta gaataataac 38640 catttaggtt ttgaaaattt acctaaaaat gaatgtttcttataatagtg taactgtcct 38700 ttcctagtgc cattaaattc tcatacctta taatgtgttacttttaatgg atttaatcct 38760 aaatgatgtt ttacacctga ctcatatttg ttaattcagatttatccaga aaactaaagt 38820 ttgtagtagt gttgaataat tttcttttgc ttctgctttcacttgaactc tgttgtggca 38880 taacacaatg gacctctacc tcctcttccc cctgctccccctgccatatc agtgcttctc 38940 aggaaatgtg aagtgttttt actttagaga tcactgtaaaatgttatact tttaattgat 39000 tatgcctgcc tttgtgtgta tatgtgaaaa tacttctcttatgtaactac cttcttttaa 39060 aatgtaactg ctacaactgg tctactaccc ttgttcaactattagtagca aagaatttaa 39120 ttactgtaat tttttgacat tgattggccc ctctgttacatattttctcc tccacttttg 39180 ntnttttttt tttttttttt tttttttttt ttttttggaaacggagtctt tttcacccag 39240 gctggaggca tgattttggc taaccgaaac ctttgcctcccgggttaaag cgattgtcct 39300 gtctcaacct cccgagtagc tgggattaca ggtacccaccaccaccatgc ctgactaatt 39360 tttatatttt tagtagagat ggggtttcac cattttggccaggctggcct ttaactcccg 39420 aactaaggca gtacgcctgg cctgggcctc ccaaagggctgaannnnnnn nnnnnnnnnn 39480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 39540 nnnnnnnnnn nnnnnnnnnn nnntagtcct agttcccaatatcatgagaa ccaactgaaa 39600 tcagggggca aaagaccctg aagtcaaggc tgccaatgtgatcctcttac aacatattcc 39660 cctatttgca cttggaatat ctggaaatct cctcacttcaccattctact gtgcctcagt 39720 ttcttatttg tatggagaat cctgatttcc atgttaaattttttagctat taggctctct 39780 gggagcaatg atcacttttt cagacaatgc agtattctggcatcctgggc aaaaaaaaaa 39840 aaaaagcttg gggtgggact ggcttatctc aactctctgtcacttctact atttcttaag 39900 ggcagaaagc aaaagtagaa aaaaaaattc ctgcaaataccttcccttat catcatttat 39960 gattggatgg ggtcataagg caatcaagca actgcttcttgcactcattt ctctatcaac 40020 aggtatttac tgagccccta catctgtgct aggatctgaggaatccaaaa gcaaaaacaa 40080 tgaagacatt gttcctactc tctgtaactt catgatgtaatggggaggtg gggtttctca 40140 aaaagcccaa ttaagaacag ctaaatgtgg ctgggcacagtggctcatgc ctgtaatccc 40200 agcactttgg gagaccgagg caggtagatc acctgaggtcaggagttcga gaccaccctg 40260 gccaatatgg tgaaacccag tgtctactaa aaatacaaaaattagctggc tgtggtggca 40320 tgcacctgta atcccagcta ctcaggaggc tgaggcaggagaatcgcttg aacccgggag 40380 gcagaggttg cagtgagcca agatcatgcc actgcagtccagcctgggtg ctccgtctca 40440 aaaaaaaaaa aaaaaaaaaa gcggccgagg caggcagatcacgaggccag gagtttgaga 40500 ccagcctggc caacatagtg aaaccctgtc tctactaaacatacaaagat gagcccagtg 40560 tggtgacaca cgcatgtagt cccagctagt cagcagactgaggtaggaga atcgcttaaa 40620 cccaggaagt agaggttgca gtgagccaag accatgccattgcactccag cctgggaaac 40680 aaagtgagac tctgtctcca aacaaacgaa aaaacaaacaaaaaaacagc taaatgtcag 40740 attatctgat actgacaata agagaagaaa taaatcacacaatcttagaa ctgtaggaaa 40800 tccaggaggc catctaactc aacctttcct caaaagcagaaatcttccaa catccttgcc 40860 agggtcatta atgttctgct ttaatgtgga actcacatctcacaaagaag ttcatttaat 40920 ttttcaaagt ctcttattgc tagaacattt tattgtaatttgaaccaaaa ctctctcact 40980 tattattcta tacagcagtg tttttcaaaa ctttaatttttaaagcatgc aatgtgtttt 41040 taaaagaaat agtaggcacg aacacaatat ataaagcagttaaaagtgga actcttctgt 41100 tggcagctgc agggggccct cagcatgtcc acttgtctctggctcttctc tacctccctg 41160 cagcccaagg ctcaccacaa aatccctgaa gctctgcacagcacaatttg aaaccactgc 41220 cttaaaggta attagagtaa atctaattcc agctgcatatgtcagccctt gaagacagat 41280 atcttgtctc tctttaaatc ttttctagat taaacttgcccatttcaaat gatctattcc 41340 atctatgtta ttttcccttt gtatctcccc catcctaatcattctcctcc taaaaatgtt 41400 gaaaccctat ttgaacacaa ttcttttgat accgtaaccagctgaggata cagcctctct 41460 tgactatctt ccggacacca gcagctgaag agggcttagtgaatggagaa ggacctgagc 41520 cagaccttga gacatatagg atttaatagg tagagatgagaaaagataaa attgcaggct 41580 gggaaactag catgaacaga gatacagaaa ttaaaacaagggcctcctga tacattttta 41640 atacttttct tccagatcct tatgggtttt tttccattcagccagctgcc tagtctctta 41700 attatttctt ggaagaaaat taagccagat cttatctccatgaacatagg cttttactgt 41760 attgctgtgt tggggccaaa ttagtgacat gatctgatcaatactaaaat taggtcattt 41820 caatattaca gtcattgcta cagggaaaag agtatttaatgaagatgttt gataagccca 41880 taaaaaaaaa gaaaaactgt gtcatcttcc aattgagtcaacatcatggc gagaggattc 41940 ataaaggtta taacactatt gagaagcatc cctgaagagtctcagttcta aagccaaccc 42000 aaagaacacc ttgtctccac aaaataatct tttgcttatcactcatcaaa ccacaggttt 42060 gatatgtgcc ccaaatagac tgtaaaaaca acagcatttcaatataggtg agtgctaatc 42120 tatcaagaca aatcatcctg taaaaacaaa tataaaaaaaaatggtttca ataaatatgc 42180 ccatcaccgt ccattttttc atgctacttg attttaccatttacagatag gaattcaagc 42240 tcatcagttt tttatgtaat ttggtttttc attcaggaaggaagatctta aagacagtat 42300 agttcactat ataccttttt cctataagat acattctttactgattatat gaatatgtac 42360 tatttcacct tacaagaatg ggaatttcgt acacacagcatcacaaagtt gggaaagtga 42420 acttgtccta aattttaatg tgttatgaat ctaacaattttaaggcatta aaaaaaactg 42480 atcttactag tattcagggt atttgattca gacattttatttccattatt ttctcttttt 42540 ccttcatctc aaaaggagat aatctgaatt gctctaatctgaaaaacaac aataagtgta 42600 gcaaatactt gacaatgaga tggaaagcct ctaaaaatctgttaccttag taacagcaag 42660 aatgtggaag aataagaata cactgtcagg acatcatttcagtttctaca gatttgaggc 42720 ctaaatacag tcagctttca ttagcttttt gtttttcccagcaggtcctt ggactttgaa 42780 acaaaacctg aatgacagct atgagcaatt ccagcctggctcctgattag ctatgccaaa 42840 caaaacaact ctctgcagtg taaactttcc aggcatctgcaaccaagcct ctgtagagac 42900 tataattcct cagtgagatt tgaaggagag tcagaggttggggggcgtgg ggcgggggtg 42960 aagggtgacc aggagggggg gagggggaga aaggataagaccttcccctc tacctcgaac 43020 tagtcccctg aactagtttc ggattaaagc agggggaaactaaagtagaa cttgtcttgg 43080 tcttgttgtt gttgtagctt ttaactctgg cttattttacagtttaatta accaatcctc 43140 taaatccgag tattctattc caatgaacat ccatgacacccttaatatca gtgtatccaa 43200 actactcatg catttaaagc aaataagcaa ttctgagaccttattagcat cgaatagagt 43260 aaggagtaga ttaaagtgat ccaaaagaga agaggaacaacaattataag tggatagtgc 43320 cctatatgga tgcaaactaa gattttgtgt catttgttgtcatcgttttt tttttttctg 43380 gatgcagatc taaaattaat tacactaaat aacagcaaactgtgctgtgt ggtcccatct 43440 gctacataat tagatcccta aggaactaac aattaagcttttttcttgtg atcaggccct 43500 ttgattctac ctaaacaaca tacctttatc tacacaaatgaattataggt tagatctcaa 43560 agcagtttat tttgatcaga tgcttgattt tgaagttatcagtggtttca gagaaaattc 43620 tgcactgggg agtcacaagg caattcaact gtaaagagatgagaattaaa ctaaaaggta 43680 gacaaaatct gccaacaatc ttcctataac ttcagaagtgaaaatgattg tttttgtatt 43740 tcttggtcat acaacaaaat ggtagcagtg aaggttgttcttgtcttatg aaaaatgaca 43800 cttaactact ctctgatctc tattgcttct gagataacctacaaaaactg atgactgact 43860 gacttagaat gctaggtgac agagatccct acttctctagtcaataacca tttattaaac 43920 agtgtcacag tgagtaggta gcatttaaag taaggagtagctctaccacc ctaaaatgta 43980 atttgcagat atatgacaac agatatcaca gaaaatttaccatttacata accatatcat 44040 aaaagtctaa cttagattta ggacaacatt aaacttaagtcattgaaaat tcaaatggaa 44100 aattcaagtt gagacttaag aaaagattct atataaagatttatcaaaaa tcacaaccag 44160 ggagatatga attctggaaa ctaaagacaa ggagatttcaatgtttaaga caggtaatac 44220 acaactagaa aactacatgt ccaattattt tattcaatttttctgtaatc tcagcacaga 44280 aactacagaa aacaacctta aggctatttt tgtaatggacgaagacaagg atagtgacct 44340 accaacaagt gaaaaccctt caatatgagt ctgtgcttgcaatcccaaac caaacgtgct 44400 gattcagcac aaagtcatca atggagttct tatcttttcaaggaacaatc cattgtctgg 44460 ctgaaacaaa gggcagtgca caggaaatca gccaaatctgggccagatgg ccactgattc 44520 aagtttcatt ctccccatcc cccaccttcc ctggcactgcaaaaaaagat gcaggcagca 44580 ctttcagtca ccaggtaata atctgcatgt ggggtataaaccttttacaa ttacatatac 44640 tccccacctc aacaccatgt aatttgttaa agcagaatgtccttataaaa tgtttaatga 44700 ggaagatcta taaagcaatt cttgaaaata cccctgtaagcccatcgttt cttaggcatg 44760 gcatgaagtt ttagcagacg aaatgattaa aaatattcatactaacttgt tctcatctac 44820 tgtttttctc aaaccatgac acaaactaga aatgaacccagagaaaactg ctttatcatg 44880 gccatttcca tgggttgata tcactggccc accgccttgctaagaacttc taggttttgt 44940 aacctagaaa cactttgtac aattacaatg acaaactactaatacaagta attagaataa 45000 tgattaatac taacttccaa atcatgttaa gaaacataaaatttttgtta caaaatatct 45060 gtcttaaact aactgggctt tgatccaaat gaggtagaacattgctttct gtgtgaaata 45120 acagaaacaa ctgtttggga aagcagaagt gtaacggagcaaaagtatct ctaagcacgt 45180 aatcttataa aaaattttat aaagtattca catactccatctgggatggg aaaagcccaa 45240 ccacataagt aaccccataa ggtctgtcat tcaaaacagagaaagaattc tagtttttaa 45300 agttcacttt ctccaagcct cagaagttaa gaggaatggcaaggaagttg gagaaggagc 45360 aaaaaaaaag atctgtgttt gaaaggagag gtagttactacccctaagaa tgatgccaat 45420 aactcctgtt ctcaaggaaa tcacccctac atattattctggaatcacgg agtaaacagg 45480 agaaaaatgc tagtggattt tcataaaaac cagtgtgtctgaccgtgtgt catagaatct 45540 gtaaattagt atactgctat aaatacacaa tttgcatccttactggttac tataaaactc 45600 aattttaaat tctaaccaat aactattaat ttaaagcaaatcatacagaa ttatgtcata 45660 cttaaatcat ggaaacatgt atattaaaga atggcataagtaaaaatcta agacctcaaa 45720 atgttcaaaa cacttttact tttcctagct ttataaaataaaggtattta gaaaataaaa 45780 aattaaaata aaataaaggt atttagaata aacaaaagtcttttctttga tggcaaccct 45840 tgactctata ggggttgctt gagaaatatt tacatttatgtggcttaagt aaattttgtc 45900 aaccaacaaa tagcaattcc tttagtaaat tttataatcctttttataat tactttggaa 45960 atgtcaatgc aggagtaaat tttattggca tgctacagtgtcgcttttcc aaaatttaca 46020 tatagaaagg ttagggtaga tcaaggaggt tttctgcctacttgtttgtt tttttaacac 46080 aagataatca caggctggta atgaaatgac taaagacaaaaatgacttga aaatatgcaa 46140 tcaacagcaa ctcacacaaa tggcctattt tattgtagggaagttgaaaa ggctgctaaa 46200 agcaacagaa ggtaaccttg cttaagagaa cattcatggtagctgacaca atcccaacaa 46260 caactatgaa ataaagcaaa attacagagc ttcaaatcttgacctgtttt aaagagaata 46320 gcggtaagca gaagcttcct taaaaacttg tcctcaagaattgctcctgg aagtttattt 46380 ataattacag tatcagacta agtgtaagac taaattttaatcagtggtga agaatatatt 46440 caagccagca actgtgtgtt ggaaaaatat atatatagtctaaatattta catagtatat 46500 agatatgtgt gtgtcataca taggtcaatg tgtttctgctaatgttagca agctttttca 46560 cagctcagat ttaattatta gaaatgtatt tccacagcctgatttctcta taaaacacta 46620 ctgttttcct ttacatagaa ctaaaaaata atactcagtatcatactaga aactttaaaa 46680 cgaggtaatt gagtattggc tgtttaatag gttttctttctttagtataa aaccgatttt 46740 agtattcatg aaaaaaagaa aatgactatt caaattaaaattctctacct tctgagctca 46800 actttagagt ctctaagata aatttacatt taaatgaaacacacacaatg gcagctggac 46860 tatcataacc accccacaca cattaatgtt ttcagagatttaaatctcta attcttagga 46920 gaaagaaaat tgtgactata caactatttt taatcagtcatacaactatt tttaattaga 46980 tttatatgtt aatgctttaa atataagagt taagggaaaagtatttctaa acacctaaga 47040 caaaacagaa ttactactaa caaaagtcaa ctcggaacaaatggtccttt gcatgttaag 47100 tctctggaaa accagctact gtgaggagct aaaatttagcaggctaactt aagattttta 47160 gtggatccta atataaagga ctaacagttt aaagctaaaatattattgta caaggaataa 47220 gttctaatta atctttcagt ctctttcctt tttttccctccttaatatgt atcttctttt 47280 ctctgatggt ccacaaatca ggtagaaaat ttaatttcattcaattaaaa ggaaatgtat 47340 ttaaaaataa catacttatg gtaaatattt tgtctattgttttttaatac tgctcatgat 47400 gagaaatata tcaaggacca aattagagta cacacagctttcagtgatat aaaattgctg 47460 aaaatccaaa tttctaaaaa gtgaatttat aagaaaatgctttaagatct atgtttcaaa 47520 tatttatttc tcaatataat ctacatcagt agaatagttatgtactaatt aatcgtgtac 47580 aatgtgcttt aaacatagtc tgaaggtcca aatgagctttcattcatttt ctttctaaat 47640 gataatttct catgccttaa atttctatat aaagaacatctcaaaagcgg tatactaact 47700 aaagttagtt tctgctcaat aagacaaaaa aaaaggatatgagttgaaca gtggtcaaat 47760 tcataccccc accatgaaaa aaaaggaatg ccacagttttagtattattt ataggtacct 47820 taatatccat gaagtgtcac taacaactta aggatcataccatgattttc ttaacatcac 47880 caataccatt tcaaatttta taatacgttc gatgcctcatgaacaagtta aaaacatctt 47940 ctagttttta aaaaattaaa tgggcataaa attccatttgatataagttc aattgaattc 48000 ttttgtaatt taggtattgg gaaacaggac aaaaaacacctttacatatt atgttatata 48060 aatattttcc tatattagta atggtttgag gaacatggtttgaataaaat gagatctgaa 48120 tctagaaaac cacaagtcac attactgtgt tttaactagactgtcagttt ccccaagggc 48180 agggacagct cttcattttc ctctctctct ccacagcatcccttccaatt ataggtgctt 48240 aaaaaagact tattagctta cttattgtta atttcctgcccattggtgta ttctattatc 48300 cccactgaat aaacacctct tgcacagtag aaaaattatgagaaaattat ttttaaagaa 48360 gtgacaaatg tatttcatga aatcattcct tttacccaaatgaaaactga gagacagttt 48420 gataattatt gctgccaatt attacttcaa atcctttttaaaaatcagta caggttaaac 48480 aaaccttaat ctaggataga attatggtgt ctgtttgcaatatgtgaact ttagctttcc 48540 aagggtaaac accatacaga tgtctggtaa tctcccatgcaacagtatct aaaataagcc 48600 cacactgttt tgattttgga cgtcttgtcc atttaaaatctaataagcaa tatgaattgt 48660 aacatctata ctgtacagga aatgtcacat ttttcaaaacacttttttcc agactatcaa 48720 ttccatggct ctattccact atgcagtatt atcctggaaatacacggctt agactatttt 48780 ttattaacta ggatatttgt tggcaaattt aaagcccatattacttaaat tatataggta 48840 catttgtaag taagtaaatt tgatgatcaa ttgaacacgcacgaaggatt ataaacacat 48900 catcattttg tgtaattcaa aaacaaaagg gaaatgaaatgtatctccct tcacctctgc 48960 cacagaattg ttttgaattt atttttcctc attaccatcctttatttaaa agataatgct 49020 actgaactag cttccaaagg gcatgggtct tgtgttggtatttccagtca acgtaataag 49080 cttatgttgt tccattaccc tcccttgccc caactcctgtgtagtcagtg gcttagatca 49140 gaaggaagtt tggcctaggt taaggatgta atacatagccatggaagaaa gctaacagtt 49200 cctacagacc tgctactatt atttgtcatt aacaaatatttattgagggg gcaactatgt 49260 gcttggtctt aggtgctggg gatagaacag tgagcaaaagggacaaagca cttgcagtct 49320 ggtgaataaa caagatctga ctcccactgg caccattccttgccaaccaa aattgagcct 49380 tgtacagcct gttacccaaa aatatggcac taaatggtcccctagtggta tgtgcaaagg 49440 ctaaaatgtc cctcggcagc tacctatagc accttcagcaagaacagcaa catttgccac 49500 aagttacctt tctgggtaat ataacacatt gtaagatgcaattcctgaca tcccaatctt 49560 ataatctagt tgaatcacaa aacaggtacc caagagtatgacacagaggc aggaacaaaa 49620 gttttaaatc agccagactg agatttgaat tctgactttacacttagtac ctgaaggctc 49680 ttgtgaaatt tacctaagcc ttatctgagt cttagttatctcagctgtaa aggtgagaat 49740 aatgatgcct actttacagg gctgttgtga ggattagaaatgatatgtgc acaatgtgtg 49800 aggtggggta catttttaat aatattgttg aattaaattattaatttctg ctattaatta 49860 aatcaataaa cagcagaaga ctaagcagag aagacctttatggcagtttt ttaaatcaaa 49920 agctgtgttt tgtgcataca cacatactct gacatacatatgctcccttt gaaattccag 49980 atatgcaact atgccatgtg ttacactgtt ccatcacagttcctactctg gaggcagaag 50040 ttgagataat gcataccact ggctcagagg tctttatgatggcataaaac ctcaaatatt 50100 tagaatacct gaaataccat aaccagacac acatttacagactctaaaag atcttcagta 50160 gattatgcaa atggtccatt taatacctac tgatatgaaacaattctgat acaatttttg 50220 accaacaata ctgtaaaaaa taccacagtt agtattttattgctccatta aaatgaacaa 50280 gggggggaaa ggttattttt acatgttcta atttttccagaaaagaacct agtaacattt 50340 ttaattcaga ggcaaacgct taaaactaag cgttttaattgcggctgggc acagtggctc 50400 atgcctgtaa tcccagcact ttgggaagcc gaggcgggcggatcaccagg tcagttgaga 50460 tcgagaccat cctggctaac acggtgaaat ctcgtctctactaaaaatac aaaaaattag 50520 ccgggcatag tagcaactaa ttgctactct ctaataagtctgagacttgt cattcaaatt 50580 caacaagtct aatgaggaaa aataactaac atttcctgagagtctttttt tttttttttt 50640 tttttgagac ggagtctcac tctgtcaccc aggctggagtgcagtggcat gatctcggct 50700 cactgcaacc gccacctccc gggttcaaac aatcctcctgcctcagcctc ttgagtagct 50760 aggattatag gcgtgtacca acaaacccag ctaatttttatatttttgta gagatggggg 50820 ttcaccatgt tagccaggct agtctcgatc tcctgacctcaagtgatctg cctgcctcgg 50880 cctcccaaag tgctgggatt acaggcgtga ctgggagtctattttgtacc agccatcatg 50940 cttggcagca ttgattcatt atctcagaaa tgtgacctgttttggaatca aattatgtgg 51000 caactgtctc agagtaaaaa atatcaagag tctcaggaaggtagttattt gttcaataga 51060 gattttattc ttaatagatt agattgattt ttgtaccaaaaggtaatgag cctaccagag 51120 aatgtagttg ttttcacatc accctgggga atgatacagtctgtgaaatt atgcacaaag 51180 agaaatattc ctcaccttag aactaagtga gttgctgctagtcatgacta ccctattact 51240 attccacctt gatcattcac tgtgctccaa cttgtgaaaatgtacaccta actattctct 51300 aatgttgcca agatcttaac ctcctgctcc agcgattttcaggccattat taaatcatca 51360 aagtagagca gccagaacag ctacctgaat tctctagttaatctggagaa gaaccctggt 51420 tcaagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 51480 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnatttt tgtattttta 51540 gtagagacag ggttttgccc tgttggccaa gctggttttgaactcctgat ctcaagtgat 51600 ccacccacct cgggcctccc aaagtgctag gattacaagcttgagccact gtgcccggcc 51660 acttttaact tttttttttt ttttttttca gatggagtttcactcttgtt gcccaggctg 51720 gagtgcaatg gtgcgatctc ggctcaccgg caacctcccgcctccctggt tcaagcgatt 51780 ttgttgcctc agcctcccga gtagctggga ttacaggcatgcgccaccat accaggctag 51840 ttttgtattt ttagtggaga cggggtttct ccatgttggttaggctggtc tcgaactccc 51900 gacctcaggt gatccatcca cctcagcctc ccaaagtgctgggattacag gcataagcca 51960 ctgcacccgg actaatataa ctttcaaaac aaatgtttctgcacagattt ttcaaagaac 52020 aaataaacta cttctggaaa ttttaatgtt gattaaagttctatagtaat atcaagtagg 52080 tatcaagtga tagtttacta ttatttgtaa cttgcatttctaaacaacta ataaggctaa 52140 gcatttttca tacttattga ccatttggaa tagtggttctgtgaatagct agtgacaatg 52200 tttgcaaaat actttaaaat tatgtttcat aaaatattttattaccattt tataagagtt 52260 ccttataaac tagagatggt aatccttttt tttttttttttttgagatgg agtctcactc 52320 tgcctcccag gctagagtgc actggcacaa tcccagctcactgcaacctc tgcctcctcg 52380 gttcaagcga ttctcctgcc tcagcctcct gagtagctgggattacaggc atgcgccacc 52440 atgcccagct aatctttgta tttttagtac agactgggtttcaccatgtt ggtcaggcta 52500 gtcttgtact cctgaccttg tgatctgccc accttggccttccaaagtgc tgagattgca 52560 ggtgtgagcc accgtgccca gccgagatga taatcttttatttgcatatg gtacagattt 52620 ttttccccaa tctatcatat gtctatggca tttatttatggttcttgctt tacaaaaatg 52680 tttttaaatt gtgttatcat atgtctatct tttaatatatacctcttgtt tcttgtttgg 52740 cttaaataaa tgcccttcaa tgaagtaatg gttgtttaactgtggaatac agatgctata 52800 gattattttt ataggatatt gtataataat ttaaaagaataaagacaagc tataccagtt 52860 aacttagagt tatgttcctt gtgttttatt gaatattaggtgaaaattag aaagatatga 52920 tagcacatat acagtactaa aatatatata agggtacatgatcatggaaa aagttgtaac 52980 aacataaata gatgagtgac attgttggag tgaaaagaaaggggaaaggg aaaagacagc 53040 cctcaaattt ttaaaaaaga agtaactaca gttttcgtttgtttgtttgt ttttagagat 53100 agagcctcat tacattgccc aggctagtct cgaactcctggcttcaagtg ctcttcctcc 53160 cactttggcc tcccaaagca ctaggattac agacatgagccactgtgcct ggcctacaag 53220 ttttttaaaa agcataatat gatcctattc atgtaagattattaatattg attatatata 53280 aatatgcatg gagatgcata aaagaatggc taccaaaatgttgatgatgt ttgacttagc 53340 gtggttagat ttcaaggaga gaaaaggaag aagaaaggaaacaagaggaa aagaaagaaa 53400 acatgagaaa aaaaccaaag agatataaat gtgccctttccaaaaaatag aacagactgt 53460 atgggtgaga ggcagaactc tcaagtgaaa aggttcccccaccctctgat gtggatacaa 53520 gttatggtaa cagactcaga gttccctgaa taccccaaacagatatacat gttaacttat 53580 attagtgtcc atttaaattg gtttaaaaat acagagagatattataccaa ttacatcttc 53640 tcttcacact aacctataca ggcagaggtg acaacttcaagtgcatctgc tctaaaaata 53700 actaaaatta cagaatgata catttgagta gtagttctaagacttatgtc cataaaaacc 53760 acctgggaag acacttaaaa tgcacattca tcctcagactatggcagaac atttttttta 53820 aatgcacatt catgggtccc accctagaaa ttcttattagtgggtctagt gatcaataat 53880 ccgtatttaa caagcacccc aggcaaatgt ggcaggaggaattgacagat cacgctaaag 53940 aacagtgaat ttcaggatgt ttgatctctt tacaaactgattaaccaaag aatctgagag 54000 tgtggtgcac tagagagaac aaagaatttg aagtaaaaagatctagatta cctctctgac 54060 ctaatccccc actcgctccc tcgcttacct ctccccatctgctcccaggc ctcctagttg 54120 cccctctaac aacaagctga aaccctcttc ccctaacctttccagtggct cagtccctca 54180 cctctttgtg tcttcactca agtcacttct caatgacacccgtagaaaat tgcaacctcc 54240 agcttacttt ctaattcccc tttctgctct acttttctctctggcacttc tcactaccta 54300 aaaaacatca tattttcctc aatttctttt gttcattgtttgattctcct gagaacagag 54360 atatttgtca cttttttttt tttttttaaa gacagagttttgctcttgtt gccaggctgg 54420 agtgcaatgg cgcaatctca gctcaccgca acttccgcctccggagttca aatgattctc 54480 ctgcctcagc ctcccaagta gctgggatta caggcatacaccacgatgcc tggctaattt 54540 tgtatttttt tttttagtag agacagggtt tcaccatgttggccaggctg gtctcaaact 54600 cctgacctca ggtgatccac cgaccttggt ctcccaaagtgctgagatta caggcatgag 54660 ccaccacacc cggccagata tctctcactt ttcttccctgcagcatcccc agcacttaac 54720 aacagtacct ggacagaggt cagatttaac ttggccaagataaaacaact gaagcttgag 54780 caattcagtg atagaaggca gataccacta gtaagcagacagtttgttaa taaagattcc 54840 tgggtcctgc cagagattct gattcagtaa gctcgggatggggcctgaga ttttgaatat 54900 ctaacaagaa tccagctgat acggatgtta tttagagagttctcaagtgc ctctgctcat 54960 gttttagtga aagtcaaccc atcctactat aagagctgtaggctaaaaag ggaaagctct 55020 gtcatactca tgtgcgtttg tgacaagaga aaaagtgatccccaaacccc ttgtaactaa 55080 gttatctacc taaataaaaa aaaaaagatt ctacaatagaaatagtttca cgacatatgt 55140 aacactattt tcatagatat ttactacaac ttgatttttaataacatggt gtttaggtgg 55200 tttctaatgt ttttactttt ataaataatg tttcaagtgatatttttgct atatgccttt 55260 gtgcatttgt ctaattgttt ttttctaaca aattccaaaaagggaaattt ccaaatcaaa 55320 gagtttagac cagcactgtc caacagaact ttctccagtgatggaaatgt tctatatcta 55380 cacagtccaa catggcagcc accagccatg agcagctattgagtgcttga aatgtaacca 55440 gtataactga gagactgaat ctttggttgt atataatgttaactaattta catttaaata 55500 gccacatgtg gctagaggct accatattgg acaaggcaggtatagacatt taaagtcttg 55560 aaacataatg ccaaattact ccccagaaaa gttgtaatcatttgccatca gtcttagtat 55620 aagaacaacc atttatttat ccaattgcca acattagattttatcaatct attgaatctt 55680 tgtaatctga aagttaaaaa gtgtctgtgt gattttaatatacatttctt tgtattatta 55740 ataatgttga atgggattgt ttcttctgtc atcacactcattcaaatgca aatatgttca 55800 cccatattat ttaactcagg ggttggaagt tatctggcttccagattatt aggtccatgt 55860 gttttcttta aaatgtattg ccaacattta aattcacataaaaatgtgaa tttctagctt 55920 cacctgaaaa atcaaatggc ctagcaacag tggcccacaaccccaaaagg caacaatcag 55980 tggagcctta gaaactctgc caccctcaga ctttcttgtccaccagaact catcacctct 56040 attactttac aagtgtcttg cttcacaact caggtttactgcttggcccc tccacacatc 56100 tgagtttaca attcctacct gaattaaatt tagagagattaaaagacaaa tagcagctaa 56160 taatagctaa ttttcattcc atggttagca tgcaaaaatgatttatgata tttctggatt 56220 attttataac taatatttaa ctattaggag aatacttttttagtgggcgc tgaggtttct 56280 tactccctag aacagtgctt cagtatcaga agggttgttaaaatgcagat tggtaggcct 56340 tatccccaga atttctgatt caacaggtca gagtggaccccaagaatctg catgtaacct 56400 caagctctaa gtgcaactct acatcaagat gaggttttctcaaagatatg gacactccta 56460 gaagggtagt gacattatgt aattttgaca acataatacgaatttgtaga tccttttttc 56520 taaaatatgt ctacatttat tatttcattt tatcttcaaaataaacctat ccagaaggtt 56580 ggcaattagc agcagtaatt ggtaatctca ttttacaaaagaaaagtgaa gcccaggagc 56640 tgaagagnag gttcctaggc ccacaaaaag ttggagagagccgtaacaag gaagggttcc 56700 tcgctactcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 56760 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnc taagcaccca 56820 aaagttggga agaccggtaa cagcaggtct cctcctaactcctaaccttg gtaaaatagg 56880 caaacacggg aatacaggac acaactggtg ctgcttttgcagactggcac cataaaaaca 56940 gaactatgac caggaacaga gaagtcaata caagatatagttcacagcaa caaacattta 57000 tcaagtgatt atgtgttaat ctatgctaac atggctacagtccctactgt agggactgct 57060 gaagatactc ataaagcata gggaaaaata aaaataaaatctgaaaccac aaccttgaaa 57120 agatagtgaa ttctctacat cataggcagg tcaaaaacaactgcactgaa gggtggggtg 57180 gtaggggcaa atacagagtt gggtagaaga gaagagtcactgtgggactc cacatctgga 57240 cttgaaggag ttgctgtcac cagtcaagaa tgggagggaagaaagtgtac acaataggga 57300 gccctcctgg ggctcaacag cacaaagagc tgctgttatccatgttgggt gtatatttgc 57360 caaaaatatc agggatgctg gaactccaga actcacaagggcatgctttt tgtagtctaa 57420 attaccttga ctatataaga actcaagtca gtgggcataacttcattatc catttgtagc 57480 caaaagataa catgattgga gatcataaat tttctctctgatttttcctc aagttttgag 57540 aagcattcct agtaaaattc cagcaaactg tgatcacaatactaaccaca aatcagctct 57600 tcaaggaaga ttgcattttc acttaaatat cgtttatatcttagttaaca gctacttttc 57660 taagagttaa ataataataa tagctaataa atattaccatagactgaaaa tacaagaaaa 57720 ctacaattag tttgaaaaaa gtctattgga aaatttggttcactattcat tagtggtgta 57780 actgaccacc cctatatgtc tagcttatgg tacagctcaaaatgactgag gtaatgaaat 57840 ttccatcact cagtttggaa aactatagca atatggcggaaaaaactatc cctcactttg 57900 tataagacta aagaaatgca aagcaaaaga aaggtataaaatgttacact gattaaatta 57960 gtaacaataa tatgttttaa gataacataa gcacacacatagattgttaa tgacagtata 58020 aatttatata gtccttttag aaagtaatgt ggaaattactgtaaatctag aaatttccta 58080 ataaaaataa gagctaacat ttatgtgttt catgtgttattgctttttat cttaacttta 58140 aagaactatt aatataaccc attttacatc ttagtaagctaaagttctga gaaaataaat 58200 tacttgtcca atattgccaa tgagtactag agctaggtttaaacccacaa cctctaattt 58260 tagaacccac ccatttacaa ctacattata ctatccctcaagaaaaaaaa gatcctaagg 58320 aagttaaggg aaaaagaaga aaagaaaaga aataaagttacatgtataag aataggcatt 58380 ataggccagg catggtggct cacgcctgta atcccagcactttgggaggc tgaggcgggc 58440 agatcacgag gtcgagatcg atgagatcga gaccatcctgcaacacagtg aaaccccatc 58500 tctactaaaa atacaaaaaa ttagccaggc gtggtggcaggcgcctgtag tcacagctgc 58560 tagggaggct gaggcaggag aatggcgtga acccggaaggcggagcttgc agtgagtgga 58620 gatcgcacca ctgcactcca gcctgggtgg caaagcaagagttcgtctca aacaaaacaa 58680 agcaaaacaa aacaaaacaa agcagaaaaa agaagtaggctagttacatt ttttagtgct 58740 gcaaaatcaa aaacaggaag gcttaagtta ataatgaacatccattgcat aaacttttat 58800 aaaattattg taaataatgg gaagtaagct atagaaaaaatgtttataaa aaacagggtg 58860 aaaattatct gcaatataaa acatttgtaa aaatattcattcatatggca agattgggaa 58920 gggagctgga aaaaataaaa accattacca ttttgaagaatttcactact ggtattgaat 58980 tttcttttgt ttctgtcagc ttcattaatc aatcaatattactctaagta cccttacaaa 59040 tatattcttc tagttcttat tcttaggatc ccaaacaattcctacagcga atttataatt 59100 tatattagac ttattcaaaa taattacata tgtttcatcagcggtgcttt tagaaagctt 59160 tagctttcag tatgagatct ctatcttaac atcaacactttttgcaatca tcgcttacag 59220 tgtacctttg aaatccctta atttagacca tacgcagtcactaaaatcag ctaattttta 59280 ctttcacaac cttacaatca ctccttaact cctgtccccatcattagccc aaaggctgca 59340 ggtactttac aaattagaag tttcctaata agaacatactccctaggtga atatttatca 59400 agtaactaaa atatcttagc tataagggat gttcataatctaaacacttc agagaagtat 59460 aaattaaatt atgtcaagtc tctttcaagt aggtaaagttaatttccact ttaccaaaaa 59520 gaagagtttg gtaagagtaa ttttcctaaa aatgacactgtgggctgcga ggagaattca 59580 ctctccttag ccacacaagg tgacatgaga ggccctcagcccggtgtttg gcctctatca 59640 gcaacataga cacacattct gtgggtaagg agagtagctgttctccaatg acatcaaact 59700 cgtaagagca gggatatagt ttaaacatct ctaacctcctcttcatgaat ccaccattca 59760 ggaatgtttt ttgtcgcccc actgtctaac atccacagtccaatggttat ctttagccag 59820 ttttaggaca actattttaa tcgtagaact ctggagttcgaaggaccctt aaaaatcaca 59880 tagttcaatt cctttatcta actgagtggg aaacaaagttttccaaaaaa aagttacgtc 59940 gtgtggcggg tttgtggcaa atccaggcct agaattaagagatgcaacca aacatctgaa 60000 actcggcctt cgtatttatc ctttaccact aacatctttatattatgtca tgtcaatggg 60060 agtatacaca cgccctttca gtgttttcag atacacactgcggtagacaa agtaaactac 60120 tacagttctc attctctact tcataaagaa ttccaaaatggaggaaaaat tcaataaaga 60180 ttgcaaaaac cagtgtaggg aaactggtgt gtgtgcaggaggagatatta gtttaatagt 60240 tttgctttcc actgcatact gttctaagat ttttagccctctaactgatc ttcaaggtaa 60300 taattgtcca catttggcta aataatctca tgtcatcatgagtgggtatc aagttaatgg 60360 tcatcaccac gaatatatag ttcagtctta tattcaatccctttcatcat ctataatgta 60420 attccatttt aatagactta cggaaagata tacaaaaaatatacaacagt ggctgcctct 60480 gagaaggatg aaggcttgta tggaggagag gaaagagggcttaatttaca ctgtatactc 60540 ttcactgttg tttgaaatgt ttaccctact tatatattcattcattaaac attttcctct 60600 tgaaatacat acctatatgc atgtaactgt attactaaatatttcaacaa tttaattttt 60660 tctcttttaa ataatttttc agtatcattc acaaaacctcctagaaaatt tcagcaaaga 60720 gaagaaacaa cacagtcaca acactcaaag cagagttccaggatttaaag tctaagaaca 60780 tatatagatg aatgagaggt tttattaagg gggactgagttcaaaaacct tttgtgggaa 60840 agctcttccc tgtatttaaa cggctaggtt taaaaatgtgatttaagcag ggattcccgc 60900 ctcgaggcgg ggggaggcgg aaaaaaacac cttacggtgtgaaaagcaga aattaaaatg 60960 tggtttctat cctctcagcc ggggccagag gagtgggtggggaggataag gaccttggaa 61020 ccgtcacgtt caccggagaa aggggcaaga ctgaggagtttctccaggac ccgcagcctc 61080 ggttagaggc agcggcagtt ccgggcaaac gagtcgcgttcggggacgtt ccttggactc 61140 atgtaactga gaaaccccac gcactgaatc tccacatggagtttcctctt aatggagctg 61200 ggaagaggga cggcgggaat ggatttcctt taacgagtctccctcaccct ccccggccag 61260 ggcgcacctc actcgctgtc tgcttgtgtc agggtcagctgtcaacacaa ctctttgtgt 61320 ctcattcgga ataagagtgg tcctcagcca tgcacagaaaaatggacatg ctggccggaa 61380 gacacgcgcg cccgctcgtg acgaagtgac atttggtgttgaggaaaaaa acccacagac 61440 gccgagcgag cgcccagcca cctctggacg ttctcaactttcccaggctg aactccatcc 61500 atcctctccc cgccacctcc gcggcgccct acctgtcgcccgcccgcctg cccggcaccc 61560 cctcactgcc cctgggctcg gggagacgaa ccaggtgccccctcgccgcc tccgccgggg 61620 gaccccgcgc tactcggcgc gccgcagctc cctcgtcccacccccgggcc gtctcgctcc 61680 cgccacaaag gcagcgagta gagcccggtg acaggccttcggcctctggg cgcggacagc 61740 acggccgggt cccttcggtg ggcgacgcga ggcggcagggaaggcaggca cggctcccag 61800 gttggcactg gcgctccctc gccacccgca cgcgctccgctcacaccccc gctgagcttc 61860 ccatccctcc ggaaaagtcc gctgcggcgc ttccccgcgcggccagggcg tccagcccgc 61920 caaagggcag ctcctgccgg tcacaggcga gctcccgcgcccggttcgcg acctctcacc 61980 tcctgcccgc gccccggagt ccccgcggcc gctcacctggccctagcggg gcgcgagggc 62040 agaggaccag ggggtggccc tcggctcggc cgggctggtctccccacccc cagtgccgtt 62100 ccctgcgccg cgctgcgctc cgctgctccg gccgcccgactccgaggtgc tagcgccgcc 62160 ggcggctggc gtctctctgg caggcgcgcg gtccgcgcgctcagttcccc ggggcggagc 62220 gaggcggcgg ccgccgcagc cggaggcgga ttcctcaggtgctgcccctc ccgcccgccc 62280 tggcctccgc agcggggacc cccgcgcccc ctccccccgccccgcctcgc gccttcccgc 62340 tgcctcggcg tccgcgagtg gcttttgggg gtccctcgccctctgtgagg gtggtgggcg 62400 cgcatttctc ccctaccccc ctccaacgac gccccccgcccagcctgtgg agccctcgat 62460 gcgcggtcct cacgcctcca ttcccaaacc ccgccgcacagacgaacgcg cagccccgcg 62520 ggggtgtaca gggggtcgtg cggggggggg gtcttctgtgagggagggct gcaccccagt 62580 gtgctgacgc gcgaagccag gaggaaaaat tatttcccctggagcccgcg ttcattcagc 62640 ctgctctcgc ctctgcgcaa ttgctttctc tgttcactggacggcggctg aagagcccca 62700 agcgtgaact cggcctgggc agtggtggct cggaaacccttggccttttg catgaagggg 62760 gtagggtggg ggcagggcgc ggacaaaacc aaacaaacccgggagctccg ggcccaagcc 62820 gacttttgca cctcccggag gagcacgccc tccggccccgggccccggct cgtcggtagc 62880 ctcccgggac cacggggacg gctccacccc agggcagccgcgtccagctg gccctgggcc 62940 ttgacgggtg ctgcggggaa aaggttcttg agagatgctgcggcccggta gagagattta 63000 gctccacttt tcacagctgg aggcttttgt tattagagatgcggctccat ttttaaaact 63060 aattgttgcc acagataggc gactacatct ctggaagcagcagctccatg ctggatgtgg 63120 tggaagaatc gaggtaagga aaggctgatg tattttgagtgcctttagtg cagtacagcc 63180 cgggggagct ccccgtgtgc gtgggagtgg ggaggcagccgcggcgccca ggcccgggtg 63240 ggttggaatc attgtcaagt gataggtacc atttaaagacattgtcaatt aaactagttc 63300 tcatacgatg tcccagtact tttgtttgct tgtcttgcatttttcttttt tgccatatcc 63360 gaaatacatt tttttctttc ctgtcacttt tgatccatgcagagggaatg gactggaagt 63420 ttggaatcac cggaaaataa caaggcatag ctaagctgtgactagcaaga taaacgattt 63480 gttcgaggtt tgattgtcct ttccctcctc cactcggaagacatagttgt ctttttttaa 63540 atcatgtttt gtaccgaaat atttttattt tataattgaatccctgttgt tgggccttta 63600 aaaacccata gaaacattgt tatgtgccac gaaggtgggaaacacggctt tctcagtcat 63660 tgctgtactc ccacaattct gggtcatgca cacaatacaggtttagtaaa taaaatccgt 63720 atgtttttgg ccccacgcgg tggctcatgt ctgtagtcccagcgctttta gcctgggtga 63780 cagagaccct gactcaagag tgtgtgtgtg tgtgtgtgtgtgtgtgtgtg tgcgcgcgcg 63840 cgcgcgggcg ggctttaaaa taactacaac agaaaggtgcagatgattag caatagctaa 63900 aagaatccaa agtcacttag taaactcatc atgagaactggccatacacg tggaaaagac 63960 agtgctgtag aaactgaaat tttaagttca cctggtgaatataaactaag ctcatagaag 64020 ttaaagcagg tgagaatctt ttgaaaaact caacccatcacaaggtctgt agagtgtgtg 64080 ggggttgggc agaagcaaaa caccttccaa tttaaagataaggacactga acaaggagcc 64140 gctttaaagt aaaaccacca gtgcagtttt ctctcttagtgcaaggacca tttgttgagt 64200 gccaggtaca ctagacccta gaaatgtgaa agcaaatctggcagtcactc aaggaccata 64260 gtctaatatt acaggactct ggtattcatt cttcatccattcaacagata ttgagcacct 64320 aatgtgagtt agacggcaca atatatcagt agtagtggtgatttgtcagt aaacaaggca 64380 agtccctaac aagggtaaaa gtgagagtaa tctatatatcctggtagatt gaaaagagtt 64440 caatgccatc ttcattagat aacaaactcc cagccgggtacagtgtctca cgcctgtaat 64500 cccagccctt tggaggccaa ggcaggtgga acacttgaggtcaggagttc gagaccagcc 64560 tgaccaacat ggtgaaaccc catctctact aaaaatgcaaaattagctag gtgttggtgg 64620 cacacacctg tattctcagc tacttgggag gctgaggcaggagaatcgct tgaatcctgg 64680 aggcggaggt tggagtgagc caaattgcgc cattgcacccccagcctggg caacaagaac 64740 aaaattccga ctcaaaaaaa aaaaaaaaga tttcacactcctgtgtcctg aatttgtgtt 64800 tctgccttgt cttccctttg ggatttctat gtccctcagcatgcccagta gacctccata 64860 attataaaag atgatagggt tcagagtgaa cattctgaaaaaaggtccca tagttttgaa 64920 tcctgactcc agcctggtga cagagtgaga ctatgtctcaaaaagaaaaa aaaaaaaaga 64980 agatgcatta gtgcctgaaa cacttttttt tttttttttttttttttttt ttttttggnn 65040 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 65100 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncactttttgacc gggcatggtg 65160 gctcacacct gtaatcccag cactttggga ggcgtaggcgggtggcccct gaggtcagga 65220 aatcgagacc accctggcca acatggtgaa actctctctactaaaaatac aaaattaggc 65280 cgggtaccgt ggttcacgcc tataatccca gcactttgggaggccgaggt gggcagatca 65340 cctgaggtca ggagtttgag accagcctgg ccaacatggtgaaaacccgt ctctactaaa 65400 aataccaaaa ttagccaggt atggtggttg gcacttgtaatcccagctac ttgggaggct 65460 gaggcagaag aatcacttga acccaggagg cagaggttgccgtgagccta aatcgcccca 65520 ttgcactcca gcgtgggcaa caagagtgaa attctgtctcaaaaaaaaaa aaaaagaaaa 65580 aaaaaaaaat tagccaggcg tggtgctgca tgcctgtaatcccagctact caggaggctg 65640 aagcaggaga atcgcttgaa cccgggaggc agaggttgcagtgagctgag actgcaccat 65700 tgcactccag cctgggcaac aagagagtaa ctccatttcaaaaaaaagaa aagaaacaca 65760 ttttttaagg ctatagctgc cattggtagt gattactctggcgaatctag acaaagtaaa 65820 ttgaaaacct ctgaaaagga atcaccactt aaatgccattaagaacattt gtgatcatgg 65880 gaggaagtaa aaatatcaac attaacagga gtttggaagaggttaattcc agctctcaca 65940 gacaacttcg aagggttcaa gacttcagtt gagaaagtaattacagatgt ggtagaaata 66000 gcaagagaac tagaagtaga agcagagctc aaagatgtgactagattgct acaatctcct 66060 gataaaactt gtacaggtgg ccgagcacag tggctcacgcctgtaatccc agcactctgc 66120 aatgcctagg caggcagatc acgaggtcaa gagatcgagaccatccgggc caacatggtg 66180 aaaccccgcc tctactaaaa atacaaaacg tagctgggcattgcggtgag cgcctgtaat 66240 cccagctact cgggaggctg aggcaggaga atagcttgaacccgggaggc agaggttgca 66300 gtgagccaag atcacgccac tgcactccag cctggtgacagagcgagact ccaactcaaa 66360 aaaagaaaaa aaaaaaaaga aaagaacagg tgaggaatttttttacttga tgagctagca 66420 aagaaagtgg tttcttgaga tggactctac tcctggtgaagaggctgtga acatcgttga 66480 aattttcaga caacaaaagg ttactcgcag tggctcacacatataatccc aggactttgg 66540 gaggctgagg caggcatatc acttgagacc aggagttcgagacgagcctg gccaatatgg 66600 tgaaacccag tctctactaa aaatacaaaa cttagctgggcatggttgca catgcctgta 66660 gttgagctac tcaggaggct gaggcaggaa aatcacttgaacccaagagg cggaggttgc 66720 agtgaaccat gatcacacca ccgcactcca gccttggagacacagcgaga ctccaactca 66780 aaaaacaaac aaacaaaaaa tgacaacaaa ggattattccataaagctaa ttcataaagc 66840 agtatcaaag tttgagagga ctgactccaa ttctgaaagttctactatgg ataaaagtgg 66900 atcaaacagc attgcatgct acagagaaat ctttttgaaagagtcaatcg aggtggcaaa 66960 cttcactgtt ttcttttaaa gaaattgcca ggcctggcacggtggctcac gcctgtaatc 67020 ctagcacttt gggaggccga agtgggcaga tcacctgaggtcaggagttg aagaccagcc 67080 tggccaacat ggtgaaaccc tgtctctact aataatacaaaaaaattagc tgagcgtggt 67140 ggtgcacacc tgtaatccca gctactcggg agcctgaggcaggagaattg cttgaacctg 67200 ggaggcagag gttgcagtga gccaagattg tgccattgcactccagcctg ggtgacagga 67260 gtgaaactcc gtctcaaaaa aaaaaaaaaa aaaaaggaaagaaagaaaga aattgccaca 67320 gccccccaac cttcagcaac cacccttctc atcagtcagcagccatcaac atcaaggcaa 67380 gaccctccac cagcaaaaaa aaaaaaaaaa aaaaaaaaatgatgattctc tgacagctca 67440 gataattgtt aacattatta gtactgctac ttttttagcaataaagtata ttttaattaa 67500 ggtatgtact ttttaaagat gtaatgctat tgcacatttaataaactata gtataggcca 67560 ggcactgtgg ctcatgcctg taatcccagc actttgggaggctgaggtgg gtgaatcacg 67620 gggtcaggag ttcaagacca gcctgaccaa gatggtgaaaccccatctct actaaaaata 67680 caaaaattag ccaggtgagg tggcaggtgc ctgtaatcccagctactcag gaggctgggc 67740 aggagaatcg cttgaactcg gagcgaggag gttgcagtgagctgagatag caccattgca 67800 ctccagcctg ggcaacagag tgagactgtc tcataaataaataaataaat aaataaataa 67860 ataaataaat aaataacagt ataatgtaaa cctaactgggaaaccagaaa atttgtgtga 67920 ctcgctttat ggagatacgt tgttgcagtg gtctcgaactgaacccacat ttcccaagta 67980 ttcctgtgca gcatggatgg ctagacaaaa ggatgattcatctccctggt aggatggagt 68040 gggacggcat gagatttaat catgctactc tgaagagcgtgcaatttaaa atgtatgaat 68100 tgtttatttc tgggattttc catttaatat tttcagatcatgcttgacta tgggtaactg 68160 aaaccacaga aagcaaaacc actgataagc ggggactgcttgatacaatt tggtctcatt 68220 aaaggaatat tccctgcttt tactgaggcc aatccacactctgataaggg gatcctgaat 68280 atgttgtcaa gtcactgaca ttctcaccta gggtatgagaaggctctttg tgaactcttt 68340 taagcaccct ctctctctgg cctccagctt ttacaacatttgacaaatgt gtgtcataac 68400 ttgtttgttt tgtgactgtg atggtgagtt tatgtatcaactcagctagg ctagagcacc 68460 cagttattta tgttatttat gttgctgtga aagtattttggagacattgt taagacttac 68520 aatcggccag gtacagtacc tcacgcctgt aatcccagcactttgggagg ccaaggtggg 68580 cggatcacct gaggtcagga gttcaagccc agcctggtcaacatggtgaa accccgtctc 68640 tactaaaaat acgaaacaaa ttagctgggt gtggtggtgggcgcctgtaa tcccagctac 68700 tcaggaggct gagccaggag aatcgcttga accccgggaggcagacggtg tggtgagctg 68760 agattgcgcc attgcactcc agcctaggca acaagagcaaaactctgtct caaacaaaca 68820 aacaaacaaa aaaagacgta caataattta ctttaagtaaaggatcttat ccttgatagc 68880 atgggtgaga cttatccagc cagctgaaag gccttaagagaaaaactgag gtttccctga 68940 agaagaaaaa attctgactc aaaacagcag cagcagctcctgcctgagcg tttccaaact 69000 gctggcctgc cctacaggtt tcagatttgg cagtccccacaatcatataa gccaatacct 69060 tgaaataaga tagacagaga gaaagaaaga gagtgtgtgtgtgtgcgcac gtgtgtgtgt 69120 gtatgctgaa aatacttttt ccagttgggc acaatggctgtaatcccagc actttgggag 69180 gccgaggtgg gcagatccct tgagcttgga agttcaagaccagcctgggc aacatggcga 69240 aaccccgtct ctacaaaaaa taaaaaaata agccaggcatggtggtgggt ccctgtagtc 69300 cccagctact cgagaggctg agatgggaga attgcttgagaccaggaggt agagatgagc 69360 tatgactgca acactgcact ccagcctgag tgacagagcaagaccctgtc tcaaaaaaaa 69420 acaaaaagaa aagaaaaaga aaaaatactt tttccatctcatatcttgtc ttttcactct 69480 ctttatagtg tctttgatga gcagacgttc ctaatgtgaacttttattta tcaatctttt 69540 acctctcata gtttacactt tttaatcatc ctctactcccaagtcttaaa gataattatc 69600 ctaggtttcc tcaaaatcat aaagtttgtt tgtttgagacagggtcttgc tctgtggccc 69660 aggctgtagt gcagggtgcc atctcggttt tgcagcctcaacctcccagg ctcaagcaaa 69720 ctgcccacct tggcctccca aagttttggg attacagggatgagccacca cgtctggtca 69780 aatttaaggc ttttactttc acatttacgt tattaatccagctgaaatta agttttaaaa 69840 atatgatatg agggccgggc acggtggctc atgcctgtaatcccagcact ttaagaggcc 69900 aacacgggca gatcatgaag tcaggagatc gagaccatcctggctaacac agtgaaaccc 69960 cgtctctact aaaaaaaata caaaaaatta gccgggtgtggtggtgggtg cctgtagtcc 70020 cagctactca ggaggctgag gcaggagaat ggtgtgaacgtgggaggcgg agcttgcagt 70080 gagccaagat cacaccgctg cactctagcc tgggtgacagagcgagactc catctcaaaa 70140 aaaaaaaaaa aaagatatga tatgaggtag ggctccagttgtccacacgg ctttattcct 70200 ttacattgtt tttttcttca agatacttat tattatactatgtgatattt tattatgcat 70260 ttatttatat attgtctcct cttccactag aatgtacgctacatgaaggc aaggatttta 70320 ttctgttttg ctcatgctat atcttcagca tctagaacagtaactgacat aggacaggag 70380 caaatcaata ttcattggat ggatggatgg atggatggatggatggacag atgcatgggt 70440 ggatgggtgg atgagtaaat ggatggatgg gtggatggatggtaaataat gaatcagggt 70500 ggatggattc tgcttggttt tatagctctt gcttcaccctatgcattacc caccaaatta 70560 aaatctctgt ggcagctgtc tcactctgct ttataatcccaaggcaccta gcacagtgct 70620 ttacatttca aacatactca gtagatattt attaagtcgaatcaaatcta cctccttata 70680 acctttctcc ctcactctta tgcgtcatgc acaattctacaggattaagc cacctaaaat 70740 caggtctgat ctacatctct gtcctactca aaaatcctccagtaaatttt ttccatgaac 70800 tttgacatga aaccattgtt ttgagaaatc ttctatttattatgtacaga taaaatcttc 70860 tattttttaa ggaagtagca tatatgttat ttaatccctacaattaccct gtaagacaag 70920 taataatagc ctccccactc gacaggtgag aaaacagcctgagagaggtt taacactttt 70980 tccaatgtca gttacctaat aagtatatga tctatctgattccaagaatg taactatact 71040 acattttgct gtaacttccc tggtactcaa aaccttccacaacatgatcc ctatcaattt 71100 ttccagtatt acttttctac tatagccaga aattactattaaattttaga agtatatcct 71160 tcctatgaag tccatgtaaa tagtaaaaaa tgggaagcaagcaattggtc aaccataggg 71220 aaataattta aaagaaaatc aaaattcact gcaagaaatatagcaattga aaataatgtc 71280 aggccaggcg cagtggctca cgcctgtaat cccagcactttgggaggcca aggcaggcgg 71340 atcaactgag gtcaggagtt caagaccagc ctgaccaatatggagaaacc ccatctctac 71400 taaaaataca aaaattagcc aggcgtggtg gcacatgcctgtaattccag ctactcagga 71460 ggctgaggca ggaggatcac ttgaacctgg aaggcggagattgcagtgag ccgagattgc 71520 gccattgcac tccaggccta ggcaacaaga gtgaaactctgtctcaaaaa aaaaaaaaaa 71580 tgaaagaaag aaagaaagaa aagaaaaaga aaagaatgtctacacgtgta ctttgacatg 71640 tttcttatac ctgtaatccc agtactttgg gaggctcatgtgagcagatt gcttgagctc 71700 acaagttcaa gaccagcttg agaaacatgg tgaaaacctgtatctacaca aaatataaaa 71760 gttagccagg catggttcca tgaaccatag tcccatctacttgggaggct gaggtgggag 71820 gatggcttga acccaggagg cagaggttgc agtaagccaagatggcacca ctgccctcca 71880 gcctgggcaa tagagccaga ccctgtcaca aaaaataaaaagaaacatgt ttaaaaataa 71940 gatggattga aggatgaata gagagatatg tgatagagcatttatagtaa aatgttaact 72000 gtataaataa gcgatgagaa tataggtgtt cacagtaaaacattttcaac tcttctgtat 72060 gcttcaaaat atttaagtaa atctactgag aaaattgtatataaaaatta ctataaatgt 72120 gtaaaaagag actctgccct acccaagaaa aaactaaaggaaatttacta aattattaat 72180 agtagctgtg tttggatggc atcatatgag taatttttattttttccttt ttgtactttt 72240 tagtatttta caaatgttta acaggcatgt tacccaattctaataaaaac catttttaag 72300 aaaataagga tgcatattat ctcatacttt acaatcttatagtaaaacta gtaaaactaa 72360 caggacataa gagagagaat gagaatcaac ggatagaggcagacagataa aatggagagt 72420 tggccctaag actgcctcca tgctttaata aatataacctgaggaactta aaggaccgcc 72480 cctttttact gaaaggtctc tactgttaaa atatgtctattttccttttt ttcttgatga 72540 tcaaaaagac cacttctagt ggaaatcctt tcacactcccttacgtccta ttgcttagag 72600 ctgagccagc ccttggagcc cttggctgct gccaaccactactacccact tcgtgtgtta 72660 gtggaggcct cccagtcctg tggagctgat gatctggtaaatagctcttg aggcttttta 72720 aaagcatatt cccccagaat tctggctccc tactgttaaaataaataaat tctctacacc 72780 agcatgccag aatggagttg ggctttggag ctaaggtcaagggtagggtc aatcagcagg 72840 ggtctcacaa aaccccagag gctgacattt cgaggcagggtaactttttt aaatgtaaaa 72900 gtaagtgttt gcagattctg gaaacaaaca aacaaacttgacttggtatc ttttcttggg 72960 ggtggaggca atctgtagtt atcagatggt aaaatcataattaaacaaag ctccaggctt 73020 tatttaagga ggtaactttt ctgaggttta accttgttttgatcaggtaa ttttccgcca 73080 cagaaaaatg ggctcatatc aaacagagtc cttttaatggacaaacttaa gcacaaaaag 73140 ttgtttctaa ggtggcatct ttggcaaaaa cactagagaatcaaaagact ataaaattaa 73200 tatcaagaaa atattgaact tcaattatat aattggcaccatttcagtct tttagcataa 73260 cacactacct gtaactgcca cctggtttag attacttgagaaaaacaaac aaacatgttc 73320 tctaagtttg gtatgtatta aaaagatcac ataattcttttttttttttt tttttgatat 73380 ggagtttagt tcttgtcgcc caggctggag tgcannnnnnnnnnnnnnnn nnnnnnnnnn 73440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 73500 nnnnnnnnnn nnnntttttc tcctgctttg tatgtgtgtttacacacaca cacacacata 73560 cacacacaca taattttaat gatggaatta aaagaacttaaatacaggac ttaagttaaa 73620 cttttattct cttagaatgg ttcaactctt ttatcagttgatgtcataat ttagtagtca 73680 cataaacaca tacataaaat gttttagttt cctaagtaaaccatgaaaca ccaaaaataa 73740 aatcactggt ggggcgcggt ggctcacacc tgtaatcccagcactttggg aggctgaggc 73800 gggcggatag cttgagttca ggagttcaag accagcctgggcaacacggt gagaccccat 73860 ctctactaaa aatacaaaaa aaaaaaaaaa tagctgggcatgctagtaca cgcctgtggt 73920 cctagctaat caaggggctg aggtgggagg attgattgagcctgggggca gaggttgcag 73980 agggccaaga ttgtgtcact gcactccagc ccgagtaacagactgagaac ttgtctcaaa 74040 acattaaaac aacaacaaaa acaaaatcac cttaataataaattagtttt tcttagtttc 74100 taaaggaaat tcaggctttc attttgactc aaaatgatgatacataaaat tcttataaat 74160 tattatttgg aagccaacaa ggagggtaat gacataaattataaaagttc aagtggatgc 74220 ctgaaaaata tttttgtaac tcaggagata attgtttacatattgtgcaa ctatattgtt 74280 attgttactg caactcaggc ttttagaagg aagttttacatggaaaaaat tatttcctac 74340 ctccgagttg gtgaccaacc aatgaattta gagattaatttcagtataat ctttcagctg 74400 attactttta attaagtagt aaaaggaaac tacaaacagatgagatatgc tttttttttt 74460 ttttggtgta tgattggcaa gggacaaaaa acacatatgctgagtaattg catggtgcat 74520 gtttcagcaa agcataagga gacaccagga aataaattacaggtaaagct aatcaataca 74580 agggcaggga gttttgaaga agacagcaaa agaaagattttgttaaaagc gaatatgagg 74640 aaagttataa atgtgtatat tgttaatagc tcaaatgcccaaatacattt taagtaatgc 74700 ccttaattaa atgtaaatag caaacttagt agttacgtcaacttctttct tagtaacatg 74760 tttaaagcag attacaaatc tcttagtaac aatgtagccgcagtccttct tgaaaaaaaa 74820 aaattgcttc tcttcagttg gttctgaaag acaatatatttctagaaatg aaatttgcta 74880 ccaatgaggt cttattttgt aggtgaatta tgtgacttttaacttgcagt atgcttgttg 74940 aaagtacatt taattttcca aaggaaagtt ctccacaaggttaaacaaaa ggtttaacaa 75000 aagtttgaaa gtcctgctaa agatttccta agagctctcatcttgctcgc ttccatttta 75060 cttctgcttc tctgtggtca ctattactgt gcaggaagcaattttgcaag catttatatg 75120 gctgtgagcc atgcatgagt caattagatt gcctattttttaaatctctc taacagttta 75180 tagaacagac taaccccttc actcgcactg catctgacttggattctgcc cttttgagga 75240 agaatacatt agaccaaaaa aaaaaaaaaa aaaaggcatctcactgagat gaggacagaa 75300 agtatcaaac tcttcttcct cttctccctt taaaaatgctccacaaggcc agatgtggtg 75360 gctcatgcct gcaatcccag cactttggga ggtcgaggatcacttgagtc caggaggtcg 75420 agactagcct gggcagtata gtgagaccct gtctctaaacagcaacaaca acaaaaacaa 75480 aaaaacaaaa gctatacaaa ctcctcctga gagttatttgaaatgcacat acaatgctca 75540 gataacagca attctttagt aacagggtct aacagatcattgtcagcccc ctattcttcc 75600 agatttgttt attttaggat aacttagctg cattgcaaataactttccat aagagatccc 75660 ccttccatga atgcaggtct gtatagatag aagagaaagtgctgtaatat aacagcctgt 75720 tcagtatccg tctaggccag tatttaagct aaatataatggactaataaa aatgggtaat 75780 acaaaaacag gaggtagaat aatttttttt tcttttttttgagatggagt ctcactcttt 75840 gcccaggctg gagtgcagtg gtgcgatctc agctcactgcaagctccgcc tcgccggttc 75900 acgccattct tctgcctcag cctcccgagt agctgggactacaggcgcct gccaccacgc 75960 ccggctaatt ttttgtattt ttagtgcaga caggtttcaccgtgttagcc agaatggtct 76020 tgatttcctg acctcgtgat ccgcccgcct cggccttccaaagtgctggg attacaggcg 76080 tgagccactg cgcctggctg aataaaaata tttttaatatcctttggggg cagcagtttt 76140 gccttccttc cttttttagt tccttccttc tgcctttcatttctatttgt tgatgtggct 76200 aatattagca ttagtttgca ttccctgagc acagagcagatgcctaggtc acttgtgatg 76260 agaagtgaac taataatgtg ctgaaaaaat ttcatttggaatgtctgcct catatgtttt 76320 catcgtaaga acttacttgc ctgccttgat aactggatgctattttgttc tacatatgcc 76380 atctcttttg ggccttcagc aattgatgtt gactatttagctacatagga gcagggactg 76440 gacctcattt tactcatcat tgcagcctaa gaacttagcagaacatgaca tagagtaggc 76500 actcaataaa tattaaggaa tgaagtgact ttgggttagtttttctgatc atttacacat 76560 gcaaatgaat aaagacaact gcattgaact agccatgctaaaattagttg cccttcctca 76620 aacacaccat gttctctgta tctccctgta tttccacccatttttttatt caagtagaat 76680 ttcttatagc cagcctttta cctatacctt cctactttagattcctaatt agccctcatt 76740 tttagatgaa acaaaattgt gatatttctt cctacacacggtgcacagca tctcatacag 76800 actggtctct tatgacattg tgatggctta ttggtctaaaaggatgctct ctccctagtc 76860 tattcattcc ttaaaggaag gtgagatggt taattttatatgatggcttg actgggatac 76920 gagatgccca ggcagctggt taagcattat ttctgggtgtgtatgagggt atatctggag 76980 agattagcat tggaataagt ggactcaacg aagcagatggccttccccat tgtgggtggg 77040 catcagctaa tctgttgagg acctgaatag aacaaaattgtggtggaagg ttgaattcac 77100 tctctgactc cttaggctag gacattgatc ttcccctgccatcagtgctc ctgattctca 77160 ggccttcgaa tctggtctgg aatctacatc accgtcttactggcactcag gatctttgaa 77220 ctacaccact ggctctcctg ggtttccagc tagtagatggcagatcatgg gacttctcag 77280 tgcccataat cacataagcc aattccttat aatacatttcttcagccagg tgcagtggct 77340 cacgcctgta atcccaacac tttgggaggc caaggtgggcagataacctg aggtcgggag 77400 atggagacca gcctgaccaa catggagaaa ccccgtctccactaaaaata gaaaaattag 77460 ccgtgcatgg tggcacatgc ctgtatgccc agctacccgggaggctgagg caggagaatc 77520 actggaaccc tggaggcaga ggctgtggtg agccaagatcctgccactgc attccagcct 77580 gggcgacaga gcgagactcc atctcaaaaa acaaaaaacaaaaaacaacg gtcgggtgca 77640 gtggctcatg cctgcaatcc cagcactttg ggaggccgaggcgggcagat cacaaggtca 77700 ggagttcgag atcagcctgg ccaatatggt gaagccccgtttctactaaa aaaatataaa 77760 aattagccgg acatggtggc atgtgcctgt agtctcagctactcgggagg ctgaggcaga 77820 agaatcgctt gaacccagga agcggaggtt gcagtgagtcgagatagtgc cactgcactc 77880 tagcctgggc gacagagtga gactttgtct caaaaaaaaaaaaaaattac atttcttctt 77940 atatggattc acaaagatat attctattgg ctctgtttctctgaagaacc ctgactaata 78000 cagtaggaaa catatctttt catcattgta tagtcccagtcttagcacag gtctgatttg 78060 ttgagatcca tacaatgtca gcagaataga aatccattttctttcacatt ccaacaggaa 78120 agtcagacat cttaaccaat caattgggat aaaatgtgacacatattata gtagaaatat 78180 aatacaataa attccatggg agcacaaaga agggaatggttatttcttcc tagagatcag 78240 gattaagata tatagaggag gtaacatttg acctggccttgggaccttaa aaaaagttca 78300 ccaagtggat gttctagaag agataacagt ataagcaaagaaaagaagat ttaaaagtac 78360 ttagcatggc attggaattc tgattccagc caagatggagtaacaggaaa cagatttatc 78420 ctcccacctg aaacaactgg aaaaaaaaaa aaaacagacaaaatatatga aacaatggtt 78480 tgaagatatt agacatgggt aacagaggac agtgatcggtgcaagatgga aaacaagtaa 78540 gatgagccct atgattatcc cagtttacct agagagagtttcctagccat ggctcaggat 78600 gagggaactt agtcagacag agcccattgg tcttcctgagtggaggagac tctctgatct 78660 gagagtctca ggaggccagg attgtagagt ttacaaagaaaagtactggg gctgcaaaga 78720 gaaggaactc cagggcacaa tggctcacac ccgaatcccagcactttggg aggccaaagt 78780 gggtggatca cttgaggcca ggagtttgag gtcagcctgggcaatatggc gaaactatgc 78840 ctctactaaa aatacaaaaa ttagccaggc gtcgtggtgagcacctgtaa tcccagctac 78900 tcgggaggct gaggcatgag aattgcttga accctggaggcagaggttgc agtgagctga 78960 aatcgtgcca ctgtactcca gcctgggtga cagaatgagactctgtcaaa aaaaaaaaaa 79020 aaaaaaaaag gccgagcgct gtggctcatg cctataatcccagcactttg ggaggccgag 79080 gtgagtggat cacgaggtca ggagatcgga tcagcctgaccaacatggtg aaaccccgtc 79140 tctactaaaa gtacaaaaat tagtccagag tggtggtgtgtgcctgtagt cccagctact 79200 caggaggcgg aggcaggaga atcgcttgaa tctgggaggtggaggttgca gtgagccaag 79260 atcgcaccac tgcactccaa cctgggcgac agagcgagactctgtctcaa aagaaaaaaa 79320 gaaaagaaaa gaaaagaaaa agaagagaaa aaagaaaaaagaagggaaga tataattatt 79380 atctctatta aagaaaaaaa aaaagaagtc aggagtttgagaccagcctg gccaacatgg 79440 tgaaaccccg tctctactaa aagtacaaaa attggctgggtgtggtggtg ggtgcctgta 79500 atcccagcta cttgggaggc tgaggcagga gaattgcttgaacccaggaa gtggtggttg 79560 cagtgagcca aaactgtgcc actgaactcc agcctgggtgacagagcgag gctccatctc 79620 aaaaaaaaaa aaaaaaaaaa aaannnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 79680 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 79740 nnntgcatca gtatgtaagc atataaacaa gaatgaaaagagtaagcaaa aatttattat 79800 gaaagtggat ttcatatatt ttgtaaggtg aggggaaaagagtaggggag aaatacaatg 79860 tgttaattaa acaccattta tactttatag atctaaatttaatttgtcga catgaaaggt 79920 attcaccata attttttttt aagagatggg gtcttgctctgttgcccagg ctggaatgca 79980 gtggctccat cacagctcac tgcaggcttg aactcctgggcttaagtgat cctcctgaca 80040 gcgtcttgag tagctggggc tacaggcaca tgccaccacccctggcttct tttttttaaa 80100 tttttcgtaa atgtggggtg tcactaagtc actcaggctggtctcaaatt cttaggctcg 80160 agtgatcctc ctgccttggc ctcccaaagt gctgggattacagacatgag ctgctgtgcc 80220 tagccacaca ataaattttt aagtgaacag atatttataatatatatgtt ataatgattt 80280 gagttttctt tggctggtta gtgggaaatt tctaaaacaactttatccag ttataactta 80340 cataccataa aacacaccca ttgtaggtat gtatttcaaaagttttccac aaatttgtag 80400 agttatgcaa ccattaccac aatccagttt gaagatgtcacctcaaaaat atccctcatg 80460 cctgtttaca gccaattctc aactgctttc tatctctaataattgtatct ttgagacaca 80520 caaaaatgaa aataaatcat gttatgtatt catacagaaaagtcagaatg gacatgtacc 80580 aaaattttat gcaagggtct gtttatagca aatattaattaacattttga aaaatatttt 80640 tctgacgttt ttagccaatt agcttctatt acctatagagtttttaaacc ttccctttaa 80700 caaaaagata catgagtatt ccacataaat atggcagattgtacaaatgc atttattttc 80760 tcttcctacc aaaacccaac taaaagtata gtaaagaggtttttaaaggc atgaaatcag 80820 aaggacaaaa gacagaagta aatgaaacag caacaacatttataaacttg aaagcaaatg 80880 gacaggcaat aattgacctt gcagcaatga gaagttgaatctcaactcag caatgaggga 80940 agcttagaaa caactagttt tacacaataa accacaccacctccccaaag gcagcaccag 81000 gttaggttcc aggtatggag acaaaggtga ggttattaacaggagaaatt aactgaaatt 81060 ctcttttaca aagtcagatc aatccccaga taccttcttcacttccaggc aggcaactat 81120 accttccctt ttctacagaa agctggacag ccagagttgtgggtgggggt gccattctgc 81180 aaacaagggg attaggtgaa cattgtatat actgaaagctgagtccacct acctccagac 81240 ctcttttcca actcagctcc cagaaagata gtaaccagtcctacaccatc atgacaggag 81300 gctaggtttc tctctgtaga atctaaccag tccaaggagaaatgcttaac atactgacag 81360 taggcatttc tcagtgaaag agcgagaaag catggattacagcttcttat cagctttatg 81420 gggcactact cttaaataag agcagacaac cagaattattagacaactga gaaacatctc 81480 agacatggaa aatagagaac aaaacaagca aacaaaaagcaacctggaga aaacaggctc 81540 tgaagggaga aaaaaagtgt ttaaacactt atttcattaatacattcgag atggaagaga 81600 agacaatata tgtattaaac aataacaagt agatataaatacatatactt tacccacaag 81660 ttgaaacctt atatctatac aaacaaacct gcacacaagtgtttttagca gctttattta 81720 taattgccca aactagaggc aaccaagatg ttcttcaaaaggtgaatagg taaactgtgg 81780 tacatccaca ccatggaata ttattcagta aaaaaaaaaaaaaagaaaga aaggagatat 81840 taagccacaa aaagacatgg tagaacctta aatgtatattgctaagtgaa ggaagccaat 81900 ctgaaaagcc tacctactat ctgattccaa ctatatgacattctgggaac aggcaagatt 81960 caagaggcag taagacctat ggttgcccag aactcacagggagggagaga ggaatgaact 82020 ggtggagctc aaaggatttt tagtacaacg gtagagcacaatcaaactat cctgcatgat 82080 atgctaatag taacagatga aactatgtat gtgtcaaaacccatacaatg tacaacccaa 82140 agagtgaacc ctaatataaa ctatgaattt tagttaataatgtgtaaata ttcattcatc 82200 aactgtaaca aatatactac agtaatgcaa aatactaataatagaggaaa ctggcagagg 82260 ggttcaaaac ggtatatttg ggagcctctg tactttctgcttcatttttc tgtaaagcta 82320 acactgctct aaaatagtct acaaactaaa aacaaaacaaaaaacaacca ataaagtact 82380 gttttttaaa aacctgctaa atcaggacag ccacaggtggcatgcacctg tagtcccagc 82440 tactctcatg gctggggtgg gaggattgct tgagcccaggagtttgtgtg gtgcgtgatg 82500 atcgatcctg tgaaaaacca ctgcactcca gcctgggcaacatagcaaga ccccacctct 82560 taaaatgaac acagcaagac cccatttctt taagaaaaaaattttaaacc cgctaaattc 82620 gcagtgaaat tggtactctt acattccggg ggaattaaaaattggaagaa tcttttggaa 82680 aattattaag atattatgta tctaaaagta taactttcaactgaatactt tacttccaaa 82740 aatctaccct aaggaaaaag tacaaagtat gctgggaagggatccgtaag tacagagtaa 82800 aacactggat ctcatttaaa gtatccagta aatgataaagaaatttaggc atataattag 82860 atggaattag gcttccataa gaaattataa taataactattcagtaacat cacagaaaaa 82920 tattcatgag agaaatacta actttaaaat aagaatataaaattacatat ataattacaa 82980 ttatgaaaat aaacgtttct gcaaaaagct ataagaagttggtgggatta tagatgccct 83040 ttttctctat ttctcaaatt ttctaaaatg taatcttttctttaaacaag gaagggattt 83100 gaaatgatga gacaaactgc atttttagaa tgattttaatataaatatta gatttggtaa 83160 tgtcttaatg atttttttac tttattgaat gcttatttttacaataaggt atttgtcagg 83220 aaaattaaga aacatggagt tagctgtaaa taacactacttcataggaca tgtgttcaga 83280 tgtataggaa ctattgctat tctaatgtaa ctattagactattctaatgt taattagttt 83340 caaatttcca aaagtcttga gtttttctta atgaaacattgacattaaga caatctgaat 83400 tttaaaaata cataatcaat tggtttctgc actgagtttggcatacatat cttttcctta 83460 tgatttctga ctgatatttt attcaaatct ggactctgaaaatcataatt tttctacaca 83520 tctaccacat gactggtgtt tttttggttg catatcttttagttggtgtt atgaattgct 83580 caaggattag tattctgact tcaaagtact gagaaacagttgataccaga acattttaca 83640 cccagaacac tttacactga gtgctctcaa taccaccaagtaccaaaaat tcaggtacga 83700 agcaaaagtg tgctaaatgt taaatgtaaa attctcaacatttaccacta aacttcatgt 83760 tttgaggagg atgctgttgt aactttattt atttatttatttatttattt atttatttat 83820 ttattttttt tttttgagat ggagtcttgc tcttgttgcccaggctggag tgcaatggca 83880 cgatctcggc tcactgaaac ctctgcctcc caggttcaagcgattctcct gcctcaccct 83940 cccaagcagc tgggattaca gacgcctgcc accacgcctggctaattttt atatttttag 84000 tagagatggg gtttcgccat gttggccagg ctggtctcgaactcctgact tcaggtgatc 84060 cacccgcctc ggcctcccaa agtgctggga ctacaggcataagccactgt gcccagcctt 84120 gttgtaactt taatatcctt caatctataa gcctaaatggcttcaacact aaataaaaag 84180 tcacttgtca tggctatacc catttcgaca ggcctcatgagtttttgttg tgttttatca 84240 gcaggcccta gagtaaggag aacatttttg gcactttcttgctttcactc ttcttcctta 84300 cttccattct ctcacacatt cattgagcag actcaatgtaccggacactg aagatacaga 84360 gataaaaaat gagatccctg ccctcaagaa gctcaaaatagaattgggca gacagacaag 84420 tcacagagca tgacggtctg gtggctcatg cggtatgacagggtgcagca cggcatggtg 84480 tggatggcat gacaggggtg acagggagtt gagttttgactatgttcagt ttgagatcac 84540 attagaacat ctaggcatgc agatctccta gtaggcacttaatggaggag tttacacaaa 84600 ggcctaggct gggactagag atttacaaat cattagtacaaaggtcatgg gattgcctag 84660 gaagaaggtg acaaaagggg ctaaggattg aaattctacttaagaggcag gaaaatgagt 84720 aagagcatgc aaagcagctg aggaaaaacc aaagtgggaaactaaaagag aacagtatca 84780 acaaagaaaa tgaagcacat tttaagaatg aggcaaaagccaactctatc ataagcgcca 84840 aagaatccaa gaagacaagg atgaaaaggg tacccctagcatggcaacct ggccagggca 84900 cctgcacgag cctcacagga ggctaaatct tatgacatagtgaggaactg gtgacaagtg 84960 gacagcctgt attcaaaagg ctgggctggg ccgggcacggtggctcacgc ctgtaatccc 85020 agcgctttgg gaggccgagg caggcggatc acaaggtcaggagattgaga ccatcctggc 85080 taacacgatg aaaccccgtc tctactaaaa atacaaaaaattggctgggc gtggtggtgg 85140 gtgcctgcag tcccagctac ttgggaggct gaggcaggagaatggcgtga acccaggagg 85200 cggagcttac agcgagccaa gatcgcacca ctgcactccagcctgggcga cagaacaaga 85260 ttccgtaaaa aaaaaaaaaa aaaaaaaaaa agggctgggctgtaaaggga aggagagctg 85320 tgtaggacaa ttggtgagaa atggaggatc caaagaagtatcaaaaacgc ttcctttctt 85380 ccttttcttt ctttccttct ttctccccac ctcatctccctctctcttcc ttctttcctt 85440 ccttcttccc tttcttcctt tttttaatag aaagactagaaatgaactaa tttataagct 85500 aaagaaaaca ttttagagat aaaatgctta caataaagcagaaggaaggt gtcacaagtg 85560 gattcctcac tctgaggaat gagacaaggt taagatccaggacttactac atgaaggcct 85620 ccattttctc tgtgcagtag aagccaagtt cattttcagagagctggaaa ggatggtgga 85680 acaggcatgc tgatgtgaga gaagaaaggt taggctggccaccctttaga ggtaactgga 85740 gaggagctta tttggaatca tgtaagattt cagggcagcgttcccagccc aggggagaga 85800 ccgtgaatgt agaacgggtg ggaatgaacc acccatttcccaggttgtgt gaatttcttc 85860 aataaaactc aacaacctga gtgcaagagc aaagaagaataaatggttgg agagttaggt 85920 attgatgaag gtcagagaat gggtcttaaa aacaccgaggcctccgggac tggtttacca 85980 cggaagaggt ggtgtcgcag aagaaggtca tcagggtagaaagactggtc gattgaggac 86040 tgatccaatg ggaataaatg cataagagag gacaggtcttatgtaggcat tcgcatgtaa 86100 aaacctgacc caagacaagt tcaagataaa acagaatgagaatgaggaag gaacctcaag 86160 agcccaactg gtccattctc ctgaagctaa acggaaccaccaaaaaccat cccaaagaga 86220 agagactcta cattgtcttc caagaccacc aacagtggatttttctttat ataagaggtc 86280 aggaaactga gactaaacat cgctggtttt gacgtcacattcaacgacgt cacattcaac 86340 tccgtcactg tagctcaaat gcagtcagag acgatatgcaagtgaacaag catggccatg 86400 tttcaataaa actttatttt caaaataaga catggactggatttggtcta ggatgagttt 86460 gtcaacttct gccctagatc cctttcttct gaggtatttgttcttttaaa attgattgga 86520 agaatgtttt atatgtagag aatattaaca ctttattttatttaatcctg tcatccaggc 86580 tgtcatccag gctggagtgc agtggtgcaa tttcagctcactgcaacctc tgcctcccag 86640 gttcaagtga ttcccctgcc tcagcctccc gagtagctgggattacaggc gagcaccacc 86700 acgcccagct aattttgcat ttttagtaga gatgagatttcactatgttg gccaggcttt 86760 ttgtttatta tatatactgc tactgcactt cagcctgggcaaaagaagaa gaccccagct 86820 ctaaaaaaat aaataattaa aaattttaaa nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 86880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 86940 nnnnnnnnnn agatagacat tgtcttgggc tatttcttcaccatcattct gagaattctc 87000 ttggcttttc tattatgttg ggccttttgt ttcctgtttctttttctttt ttaaaaaatt 87060 ttatttattt atttattttt ttgagatgga gtctcacttgtgtcacccag gctggagtac 87120 agtggcgcga tctcggctca ctgcgagctc cgcctccgggttcacgccat tctcctgcct 87180 cagcctccca agtagctggg actacaggtg cctgccaccatgcgtggcta attttttttg 87240 tatttttagt agagatgggg tttcacatgt tagccaggatggtctcgatc tcttgacctc 87300 gtgatccacc cgcctcagcc tcccaaagtg ctgggattacaggtgtgagc caccgccacc 87360 cggcctgttt cctgtttctt acaccttgct tgtgatttacttctttgttt taataaagca 87420 cattatctga gtttcctgag aaagaataca tgaaaggtaaagctttgaaa ccttttgtga 87480 ttgagaatgt ctttatttga ctctctcatt tgattgatagtttagcttgg tctagaatta 87540 tcactgggaa ataattttct ttcagaaatt tgcaggtatttccttattgt cttctagatt 87600 ccagttattt ctagtgagat gtataaacct attctgattcttgatccttt gtatatgacc 87660 tgctttttct aatagcttgt agaatcctgt ctttattctttattgtaaca catttcaaaa 87720 tgatatgctt tggggtgagt ctatttttat tgatagtgttggatattttc aattgaaaat 87780 ttgaatcctt catttctaga aatttttctt gaattatttctttgataata ttcttccttt 87840 catttcttta tctagaactt ctgttagttg tatgttgatctctagaactg atcctttatt 87900 tttcctaact cttctattca gttttccatt tctggtttttttttttttaa ccctactttc 87960 tggaagagtt tttcaatttt attttccagt gtttctacttatatttttat ttatctgaac 88020 acatttttaa tttccaagag ctgtctttac atgatcctgttcttttatat attcaatatc 88080 atctcatctc tctaaagata ttgatttatt atttgacattttcatttcca catgatatgg 88140 tttggctctg gggccccacc cgaatctcgt tttgaattgtaatccccact gcaattcaaa 88200 atgtcaaggg agggacctgg tgggaggcga ttggatcatgggggtggttt cccccatgtt 88260 gttcttgtga tagtgaattc tcacaagatc tgatggttttataagagttt gacatttcct 88320 ccttcacagg cactctctcg tttgccagcg tgtaagacatacctgcttct gcttccgcca 88380 tgattgtaag tttcctgagg cctccccagc catgcagaactgtgagtcaa ttaaacctcc 88440 tttctttata cattacccag tctcggttag tatctttatagcagtgtgaa aacagactaa 88500 taacactatg aatagctctg ttctctccat atttctttttccactttgtt ttggttttta 88560 ccttttttac cagagacttt tctccagtat tcggtggtccttggttataa gttcacattc 88620 aaaagaggga cactaagaag ccgacagaaa tctttgagcacatgggtagg gctttaacag 88680 aggatgatct ggctggatca gttatttggg gaatcgctttatgtgtacct ttaggtgttt 88740 cccttggagg gttaattttc ctcagagaag atcttccaatcaccctcctg gaggttaata 88800 tctagatgta gtgccctgtg agctttaaag gggaagagggcttataggat tcagcacaaa 88860 atttacaaat attcacttaa tacttctgct ttagactaatacccccaact acaggtgtgc 88920 ctgttgtgct cacaggccag gaacctctgt ttcacctttaaagaataaac ctccattatt 88980 ttgcgaggta aaaggcagtc atccagcttt gtggaattgaagaggagatc tgggaatgta 89040 actacttcat agagtttcaa cttagccttc tattttgagccccaacttcc aactatagct 89100 gatgccacca attcctaagt ctctggaaaa tccttgtatatgtgtttgct tccatgcttt 89160 cccgtttgcc agtttagaca ttagctttct caaatctgctagatccgttg tcatatatcc 89220 atctctttcc agcttccaaa gttttgttcc tgttgtctcctttctggtcc tttatatccc 89280 tatggatttc tgtcttttaa aaaaatccca gctgggcttggtggctcagg cctgtaatcc 89340 cagcactgtg ggagggtgag gtgggcagat cgcgaggtcaggagatcaag accatcctgg 89400 gcaacatggt gaaaccccgt ctctactaaa aatacaaaaaaattagctgg ctgtggtggt 89460 gcatgactgt agtcccagct acttgggagg ctgaggcaggagaattgctt gaacccagga 89520 ggcggaggtt tcagtgagct gagatcgtgc cactgcactccagcctgggt gacagagcaa 89580 gactccgtct caaaaaaaaa aaaaaatccc tttattattatgttaataga gtttcaatag 89640 gatgcaaaaa taaatgaatg agtggaattt gtcattttcattcataagtt gactttgaaa 89700 tgttctagtc tcctacaact atattatttt atttttgcacacaactaaat gttgaatttg 89760 gagttaaatt tctcttagtg accatgcttc agtttatggtataaaataaa ccatttatct 89820 accactatgc ctttagtttt agtttagtca gaaatctgaggagcaaattg caacatagaa 89880 gggaacacac atctggcctg gccctggccc tggccttatccttctaaatg tctttaacct 89940 ccaaactcta gtgtgcctcc gagaacaagg tgatctttccaagtgtggcc ttggctgttg 90000 ggaataggaa gccaattata tgagtttcat agtccaataatttttttctt tttaaaaaaa 90060 gacctttttt ttaaatctgc ctactttatt tggtaaaacaacaacaacaa acaaaacctt 90120 attttttaga gcagttttag gttcacagca aaattgagaggaaagtacag agatttccca 90180 cacaccccct atcccacatg tgtatagtct tgcccatttttaacatcccc caccagagcg 90240 gtgcatttgt tatcactgat gaacccacat tgatacatcattatcatcca gaagtccaca 90300 gtttacatta agattcactc ttcgagtaca ttctctaggtttagacaaat gtgtaatgac 90360 atgtagccat gattatagta gcatacagag tagtttcactgccctaagac tcctctgtgc 90420 tgtttattct tccctccctc ccctcaaccc ttggcaaccactgatcttta ctctgtctcc 90480 atagttttga cttttccaga atgtgatata gttggaatcttataacatgc agctctttca 90540 gattggcttc ttctttcact tagtaaaatg catttacatttactctatat ctttttatag 90600 cttgatagct catttctttt catcactgag taataatacattgtctggat ataccacagt 90660 ttatttatcc attcacctcc tgaaggacat cttgcttgtgtccaagtttt ggcaattaaa 90720 aataaagcta ctggccaggc acagtggctc acacctgtaatcccaacact ttgggagtcc 90780 aaggtgagca gattttttga gcccaggact ttgagaccagcttgggcaac atggcaaaac 90840 ctacctctac aaaaaaaaaa acaaaaacaa aacaaaacaaaaaacaaaac aaaagttagc 90900 tgagcatgct ggcgtgtgcc tgttttcccg gctactcaagaggctgaagt gggaggatta 90960 cttgagccca gaaagttgcg gctacagtaa gccgtaattgtaccactgca ttccagcctg 91020 gggccacagc aagaccctgt ctaaaaaaaa aaaaagagaaaagaaggaga agaaggaaga 91080 ggaggaggag aaggaaggaa gaggaggagg aaagaaagaggaaggaagga aggaatgaag 91140 gaaggagaga agaggaagag gaggaagagg aagaaacggaggaagaagaa gaagaaagaa 91200 gaaagaagaa ggaagaagac gactataaac atttgtgtacggtacttatt tgttatttgt 91260 atatcttctt tgctctggtg acttaagatc tttgagctattttttaatca ggtaatttgt 91320 tttcttattg ttgagtttta agagttcttt gtatattttggataacaatc ctctaccagg 91380 tatatttttt gaaaatattt tctcccagtc tatggcctgtcttcccgttc ctgtaacagt 91440 gtctttcaca gagcaaaaca ttttaatttt aatgaagtctagcttatcaa ttctttcttt 91500 catgaatcac aactttggtg ttgcatcaaa aatttatcatgaaacctgag gtcacctaaa 91560 ttttctccta tgttgtcttc taagagtttt atggtcttgtgtttttcaag tctgtgatcc 91620 attttgagtt aattcttgtg gagggtgtta agatctgtgtctagattcaa gtttctgaat 91680 gtagatgttc ggttctagta ccatctgttg gaaagtctatctctattgta ttgcctttgc 91740 ttctttgtca aagaacagtt gactgtattc atgtgggtctctttctgggc tctctgttct 91800 gttccattga tctgtttgtc tattctttat caatatcatgctggcttgat tactgcagtt 91860 ttgtagtaag tcttgaagtc acatagtgtc atttctccaatttgttcttt tccttcaata 91920 ttgtgttgcc tattctgggt cttttggctt ttggctctccatatcaactt tttttttttt 91980 ttttgagatg aagtctcgct ctactccctc ggctggagtgcagtggtgct atctcggctc 92040 actgcagcct ccgcctccca ggttcgggcg gttctcctgcttcagcctcc caaatagctg 92100 ggattaccag catgcgcttc cacacccggc taatttttgtatttttagta gagacggggt 92160 ttccccatgt tggccaggct ggtcttgaac tcctgacctcaagtgatcca ccctcctcag 92220 cctcccaaaa tgctgggatt acaggcatga gccactgtgcctggctccca tatcaacttt 92280 caaatcagtt tgtcaaaatc cacaaaataa ttttctgggatttttactgg acttgccatg 92340 aatctataga tcaagttggg aagaacttaa nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 92400 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 92460 nnnnnnnnnn atccattgaa aagtcttagt cccctgatatactttggata tgtatcccct 92520 ctgaaactta cgttgaattg taatccccaa tgttggaggtggggcctggt gggatgggag 92580 gtgattcgat catggtggtg gatttctcat gaacggattagctctatcat cttggtgccg 92640 tcctcatgat agtgagtgag ctctcatgag atctggttgtttaaaagtgt gtggcgcctt 92700 ccccaaactc tctctctctt gctcctgctt tcgccatgtaaggtgccttc tcctgctttg 92760 ccttgtccca tgagtaaaag ctccctgagg tgtccccagaagtcaagcag atgctggtgc 92820 catgcttgta cagcctgcag aactttaagc caattaaacctattttcttt ataatttatt 92880 cagtcttaag tatttcttta tagcagtgga agaatggcctaacacatccc ccaactgaca 92940 acagtaaaat tcccaattca tctagctccc tgaggacatatctgatctaa tattgaattt 93000 gttagccctg aattgaaatt caacatttgc ttgtggatgcctactatttt tgtacatctc 93060 tagaattata gagaaaccat catgattcac tatgctttgaataattccta gcacacagta 93120 aataaatgtg gaaaaaaaat ggaaaaaggg agacataaagtgttttttaa aaaagaagtc 93180 agggtataaa tcctatcaaa ttcctgtcct gaactagaaaatttcccctt tgtgttatgc 93240 catagtgcag gatgcttgct attccaaact aggttccaataatttgagat ttcctcatga 93300 gatattttgg acattttcca gatcaaaaat gttctcaatcttctcttaga atttagggac 93360 tacagttaaa atatatggaa aaaaggaagc tacaaccttcaaatcactac ccaggtatgt 93420 cctgccctct tccccatagt ctctgtggct taaaacacaggaacagctga ggacaaatga 93480 agttttggga aaagactgca ctttttaacc caaagaagggcagagaggag cagagccata 93540 ggtcatggta ttaaccactg ccactgaatt ttcgatccttccattaagag agccaacaaa 93600 tatagaaaga gacctatttc tagcctatct agacgaagaattagatgagt acatttaact 93660 cccactttgc tgtcctgtag cccccaagcc actggctccttggcagaaaa attcaccttc 93720 ccctccactt ccagatttac atatatgggt gtagctttactctggagtag aaaggctgtc 93780 gctcaagttc tgtgtaaagg ataaaccttt tacctgtgttgacagaactc cacagggcag 93840 gccaggcaaa tgaatatgct tgttgctagg ataccacactgtttccatga cagcaggcca 93900 ctgctgtttg ctactctctt gaccactagc acacacacctgttccttaag aaatatgctt 93960 gtttcccttc attataaggc aaatatttag tagaaaataacacaactaca tggctgcaac 94020 ttctctagct ttccagaata aatataatac atcaactacaatttgatgtg tggaaaggga 94080 attcacatat ccatgtgtat cccaagtctt catatgcaataaaatgtttt aaatgattga 94140 ctgattcatt gttacattta tagcccacca tgtgccacagaagagttgag tcaacaaagc 94200 atattgtttc tgaggccact ctaacattca tcctacatgctgcttacaca gtgactgttg 94260 tattatgagt ttttcaaaga ttacacttca tggttccctgtggaactcat cttttttttt 94320 tttttttttt tttttgagac aggatctcat tctgctgcccaggctggagt gcagtggcac 94380 aatcactgct cactgcaacc tcaacctccc aggctcaagcaatcctctca cttcagcctc 94440 ccttgtagct gggagtacag gtgcacacca ccatgcctggtgaatttttt ttattttttg 94500 tagagatgga gatctcactg tgttgcccag gttggtcttgagctcctgga cacaagcaat 94560 ctgcctgcct cggcctccga aagtgttgga atataggcatgagccactgc ggctggctgg 94620 aaatcaccag tttttttttt ttctcctact ggcatccggttgtcacaaaa attgccacca 94680 ctactgacta tgactgctag ccaggaccag gactaaagtgcctaggtgca cattttaagt 94740 aggtcctcac tttcagggtc gggcatgtgc acttctgccaacatctaccc aaaggtaatg 94800 aatacctgag tcatgggtgc caaggtctgg atcatagtgatggttgtaca attgtataaa 94860 tttaccaaaa acaattcaaa tgtgcatgtc cagtagttgtatttatcaca tataaattat 94920 atctcaatag agctgtcaac aatgaccaaa caaatactctgtagacactc ctatatcact 94980 gaagtaattt tcaaatctct tataatttgt gctgtggtttggatatgtga ctgtgtctct 95040 tccgttcaac tgttaagttt gtcaaaggta agacctgcattttacacact tttgtaactt 95100 ccccagtgat cagctggatt cgctgcacat aaaaaatgttcaataaatat ttgctgagtg 95160 ttttatgaca tatatttaat atcttgcttc ctgaacttctagctttgggt cttctttgag 95220 agccaaattt tgttgtatct tatattctac catcacaaatgctagaaata caaagcatat 95280 ctctatttgc tttatatttg cccatgaata catttttttacacagctcta tgtaaaggct 95340 tgttagaggt cattaggcag tagggtctga agaggaggataaattgagca agagacaaag 95400 actgatttgt atttcctatt gcatcaaaaa taataaatgcagagcaaaaa ggggaggggg 95460 aggcaacgat ggtggcattt acaacttgtg tttacccactatcccttccc ctttctatag 95520 gatattttca ctttaaaagg aaaaaaatgt aagggaacctatttaaaatg tgggatttat 95580 gacacagaaa aggaaaaata aaataaaatt ctttccagaaaccagaaagt ctaccaaagg 95640 atgcacctcg ttccattgtg atatcaaaac atagagaaactaatttaaaa aaccacttga 95700 aatataatat tcctctgacc ccaaattctt gagcaaaaataaaaacaaga aaatgactat 95760 tttatgttaa ccaaatggaa atagggcata attctaggcaaaagcccttt gaggcagaca 95820 gaattagttc tgtcactaaa agaggtggaa agattggtggttgcctgtgg ctaggggtgg 95880 gttgggctgt gactgctaat ggatacagtt tctttttggggtgatgaaat gttctaaaag 95940 ttattgtggt gacagtagga caactcagtg aatatatttttaaaactata tgataggcca 96000 ggtgccgtgg ctcacgccta taatccccac atttcgggaggccaagatag aggccaggaa 96060 tttgagacca gcctggccaa cattaagaaa ccccatctctactaaaaata caaaaggtag 96120 ccagatgagg tagcacacat ctgtaatccc agctactcaggaggctgagg caggagaatc 96180 acttgaaccc gggaggcagc agttgcagtg agctgagatcgcgccactgc actccagcct 96240 gggcaacagt gcgagactct gtctggacac acacacacacacacacacac acacgataca 96300 agaattatat ctcaataaag tatatatata gacacacatatgcacatgtc tttatgaata 96360 tatatatatg tgtgtgtgtg tgtgtgtgtg tgtgtgtatagtctctactg aaaacccttc 96420 aacagtttgg ataaagtcca gccccttaga actgcccacacagcccttca ttaccttcat 96480 ctactcatcc caactgtaac tcttgccatt ttccaccagggaccccagga tccagacata 96540 ctaaatgatt tgcagttttc ctcattttgg acctttgcaaaagttgtttt tctgcctaga 96600 atacccttat tcctcttttt ctcttactcc tgttagtttttcaaaactca ggttcaaatt 96660 tcatctatgg gagctcattc ctgtaccata caacatggttaggttcagta ccctctggta 96720 attttttctg tatttgtctg tctcctctat tagattataaactccttaag tcttatatct 96780 tattcttaat agctagtgta gggtctggca catgccagtactacatttat tccaaaaaaa 96840 tttaatgaat taatattttg attatactac tccttttatagtactataaa ttatttgttt 96900 gtatctttat ttcttcaata aattgtgagc tccttaccttattcatcttt gcatttctca 96960 ggtaacacaa tttcaagtat ataatagata ttctataatacaatgatgga tatatattat 97020 attacattat attattggat gaggctccgc agaaaacagttaaactacag gtatcctatg 97080 ttgacatggc tttctttttc tttagagatg gggtctcacattttgcccag gctggtctcc 97140 aactcctggg cttaagccat tctcccactt tggcctcccaaactgctagg attacaggta 97200 tgagccacca tgctcagcca ctgacatgga tttctgaaagtcnnnnnnnn nnnnnnnnnn 97260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 97320 nnnnnnnnnn nnnnnnnnnn nnaggtcagg gaaggataggagatattcca gacaatagtt 97380 aaactcactt ttcataattt atctatttgc ttctgctttactcctgttgg aaattcttaa 97440 cggggactgc tgcttttttt tttagttgga tttttatgatgattatattt tttttttgtc 97500 tttttaaatt tttttattat actttaagtt ctagagtacatgtgcacaac gtgcaggttt 97560 gttacatatg tatacacgtg ccatgttggt gtgctgcacccattacctcg tcatttacat 97620 taggtatatc tcctaatcct atccctcccc ccaccccacggcaggccccg gtgtgtgatg 97680 ttccccttcc tgtgtccaag tgttggctga ttataaggaggccaggagcc aagtaaacca 97740 cattgatttg tagcttattt aaaaaaaaaa aaagccaaccagttaataag ccttagaaca 97800 ttctgatctt ggtacaaatg cttgatgtaa tcttattttctaacattata tttcatttca 97860 gttgcttgct atattgttga ctagggactt caaaaagaactttgttttac ttttcatcct 97920 ctcattattc ttatgagccg aactgttttt ttgttttttcgttttttttt tgagacagag 97980 tcttgctctg tcgcccaggt tggagtgcaa tggcatgatctcggctcact gcaacctccg 98040 cctccccagg ttcaagcgat tctcctgcct cagccccccaggtagctggg attacaggcg 98100 cccaccacca cgctgggcta atttctgtat ttttcgtagagacggggttt caccatgttg 98160 gccaggctgg tctcgaactc ctgacctcaa gttatccatacgcctcggcc ccccaaagtg 98220 ctgggattac aggcataagc caccacgtcc ggcgagccaaactattttta aataaattaa 98280 gaaataataa taaattagca aaatcaatta cgttcagtcactacagtgat tacacagcca 98340 gtcacactga cttcagtttc ttccatattc ccattctacctatcattcac aacactgtca 98400 gattaacgtt cctatttaat atttaaccac ccactcaaatttatacagtg attccttaat 98460 gcctgccccc acaaagctac aaattttctg cttgctattcaaggcctcca agacccagtc 98520 ccattcacct gtgcaccttt accttcaatg acgtctaacaccacacctcc actccatcct 98580 ggctgtctca ttacccccta ccctcaccac gcacatttgcacctcagtgt tttgctttct 98640 ggcctttcct acctggaatt ccctccctta acctttacccttattcagat tcttacttgc 98700 cctttaaagc agtgtttttc catctctgct ttcctctgagaaaatgagat caggcagagt 98760 cttcccaagt atcagcaaag gtggtcagta gcaactcaacttaattctga acagtttagt 98820 aaacccagga gaaagactac atatagttcg ccgtggttctggaaaaagac ccaaggttga 98880 ctctcattgg cctggatagg agcacatgct cacccctgaaccaattacag cacttctggc 98940 taattcggct tgggtcacat gatcaagctt tgaaccaggggcatggggcc agtttcccgc 99000 aggtgcaggc caagaatagc aatcgcggca ttgtccctggaagaggagga aattatcctg 99060 ggcagacaaa aacagtggat gtgcgccata cattttctatctcacatacc atttaaaata 99120 ttatagaatc tctttcaaat acttaaataa gatatactttcttgaggatt ctatttaatt 99180 ttgaagcacg atggtaaaaa catgtatggg cacgatgtgccatctaaaaa agtggagagt 99240 tatattttta ttctgcttat gtaagcattg ttagtcaggtcaatcataac ctacagtaaa 99300 ataatattgc cagacagctg gcaccggggc ttatggctgtaatcccagca ctttgggagc 99360 ctgatgcagg aggattgctt gaggccaact gttccaggccagcctgagca acttagtgag 99420 agcgagatcc tgtctctaaa acaaaaattt ataagaatagtaatattgcc agattaatct 99480 tcctcagtta ttgattgaca cagatttgaa aagcactgattcatgtggta ttaagcgtct 99540 taactaaatc aagcattttg tccttgtttc ctgtgccgttagacactgat gaacttcagg 99600 aatgaagaca ttcacccttt ggcgcattca aaatagaaaacaagtttggt aataagtcat 99660 aatgaccata gttataaaaa taaacagtaa tttagctttaggctgggtgc agtgactcac 99720 ccctgtaatc ccagcacact gggaggccaa cttgggcagatcgattgagc ccaggagttg 99780 gagaccagcc tgggcaacat agcgagatct cctctctacaaaacatacaa aaattagctg 99840 tgtgttggct gggcacggtg gctcacgcct gtaatcccagcaccttggga ggccaaggcg 99900 ggtggatcac ctgaggtcga gagtttgaga ccggcctgaccaacatggag aaaccccatc 99960 tgtactaaaa atacaaaatt agccaggcgt ggtggcacatgcctgcaatc ccagctactt 100020 gggaggctga ggcaggagaa tggcttgaac ccgggaggcagagtttgcgg tgagcagaga 100080 tcgcgccatt gcactcaagt ttgggcaaca agagcgaaactccgtctcaa aacaaacaaa 100140 caaacaaaca aaaaattagc tgtgtgtagt ggcgtgcaactgtggtccca gatacttggg 100200 aggctgaggc cagggaatcg cttgagccca ggaggcggagcttacagtga gccgagatag 100260 cgccactgca ctccagcctg ggtgacagag caagactctgtctcaaaaca aacaaacaaa 100320 caaaaaactc gacagtacat ttgcaagcat aaaccaagatgggtcttgct agattcctga 100380 gcaaagaggt agaggggtcc cttgtggatg tcaaggggtgagggtcagcc acgaatgctt 100440 ttgtaaaatc agggattgtt gtgtcttcct cacatctatatcacgtgtgc ctgtatccca 100500 ggacacagct gtggaatgag tggaagaatg actctaggatacactcatga ggacatatgt 100560 ccattcagaa atcataaagt gattctgtaa gtatcagaattttataaact atctttcggc 100620 tgggcacggt ggctcacacc tgtaatccca acaatttgggaggccgaggc gggcggatca 100680 tctgaagtgt gagaccagcc tggctaacac agcaaaaccccatctctact aaaaatacaa 100740 aaattagcca ggcgtggtgg cacatgcctg ttatcccagctactctgaca caggagaata 100800 gcttgaacct gggaggcggg gaggttgcag tgagccgagattgcaccact gcactcagcc 100860 tgagcgacag agccagaatc cgtctcaaaa aaaaaaaaaaaaaaaacaac ttataaacaa 100920 tctttaattt ctagaaaagg agagatcgtg gtacccgaacctatctttgt aagtgatcac 100980 tgaatgtcct agcagcatat ggatccctaa aaaccagccaatcaatagct tagccttggt 101040 tgctatgttg ctgctgtttc cagaatacct gagctctgggcaggtatgca gcacaatggg 101100 ctatgaagct gggatcagat tcaaaggatg attcgttttcaaattcattt cggctgattt 101160 gctgaatgtg tgtggagtgg tcaaatcaaa gcctctttgggtagttttaa gtacttaggc 101220 ttgttttccg tgcttctttt taaaaaaagc attggctgggtgtggtggct cacgcctgta 101280 atcccagcac tttgggaggc tgaggtggat ggatcacctgaggtcaggag ttcgagacca 101340 gcccaaccaa cctggtgaaa ccctgtttct actaaaaatacaaaaattag ctgggcgtgg 101400 tggtggtggg cacctgtaag accagctact tgggaggctgaggcaggaga atcacttgaa 101460 tccaggaggc ggaggttgca gtgagctgag atagcaccattgcactccag cctaggcaac 101520 agagcaagac tctgtctcaa aggaaaaaaa aaagtgtcactctggtgtat cttgtctacc 101580 tgttcagatc gcttgctggc tgccatttgt gaaaattacttaaatgtggg ctgagactgc 101640 aaatgaaacg gtagtgacaa caacaaattt taatcaataaagaccacata cattgagaag 101700 gcaatatata aagacataag aaaaagaagt aaaaacacttttaacaatta cctaagtgct 101760 gtgtaaataa ggtttaagaa tggtggttga gactgagctatataaagttc aatagggtgt 101820 gatgacagag ctattacaat atgcatgaaa aatgacattttttaaaaaac aaaagacgtc 101880 ttctataact actacaccat tggattttgg ctaaaagtattgaaaaacag aaggctaaaa 101940 gagaaaattt gttaagaaaa ctaatattat aaaagaaggtaaataggcta caaataaaga 102000 aatatgagat tgaaagatgt ttgatgagtt tttgctgaacctgaattttt gcagaggagt 102060 ggtgaggtga aggtgttggg aaggggtgga ggaatctctagccctccata tcaaagaaaa 102120 gccaggattt taagcccaga gcctggaact ttcttgggactctgtaatga ccagcatctg 102180 ccactgtaat tacgaaacgt gttacctgac caacttcatgctacaatgtt agcttattgg 102240 tgtgaagcaa aacaaaattt tactttctta acatcctttaagtaggtgtt acttccctta 102300 atagcactgc catgcttggt cggggaggaa gggaaatctaaatcctcatg taacagcttt 102360 aaaaatgttc cagctattat ttttaagcct atacattttcccctctaaaa ttctctaact 102420 tcttctaagt tttatgtttt tttttcttct ttctttgatgctttaggtgt ttttaatgtg 102480 ctctcaatag aagtgccaaa tacttattag aagttctttagatcttgtct ttagaatgta 102540 taatgtaaaa ttctaaattt ataatcagaa caaaaataattttcctttaa tttgtgtggc 102600 atatagttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 102660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnncc ccttatatgt 102720 aactagatgc ttttctcttg ctctttttag aatgctggctttgtctttga cttttgacag 102780 tttgactata atatgcctta tagaagacct ttggggattgaatctctctg gagatatctg 102840 agctttctgt ctctagatgt ctagatctct tgcttgacttgggaagtttc agctattatt 102900 tcattaaatg ggttttctat gactttgcct atctcttctctttttggaac ctccaaaatt 102960 aaaatatttg gtcactttat aatgtctcgt atgtgatgtcatgtaggctt tcttcatttc 103020 ttcttcttct tgactaggtt atttaaaaaa aaaaactgtcttcaaggtta gaaattcttt 103080 aaatttttct gcataatcta atctattgtt gaagcacttagttgtatttt ttatttcatt 103140 cattaaactc tttagttcca agatttctgt ttggttctttttcatgatat ccatttattt 103200 gttcaatctc tcatttagat cattaattgt ttacttcatttctttgtatt gtttatctat 103260 gttctcttgt atctcaatgt ttctttaata tcattattttgaattctttt tcaagtattt 103320 tataaatttc cttttcgttg ggattactag agaatttttgttctttcttt tttttgacag 103380 agtctcattc tgtcacccag gctggagtac agtggcacgatttcagctca ctgcaacctc 103440 tgcctccagg gttcaagcaa ttctcatgcc tcagcctcccgagtagctgg gattacaggt 103500 gctcaccacg acgcctggct aatttttgta tttttagtagaggcatagtt tcgccatatt 103560 gcccaggctg acctcaaact cctggcctca agtgatccacccactttggc ctcccaaagt 103620 gctgggattc gggtgtgagc cattgtgccc tggagttccttctctttgca gccccagtac 103680 ttttctttgt aaactctaca atcctggcct cctgagactctcagatcaga gagtctcctc 103740 cactcaggaa gaccaatggg ctctgtctga ctaagttccctcatcctgag ccatggctag 103800 gaaactctct ctaggtggtc ttgaactcct gggctcaagtgatcttccca ccttggcttc 103860 ccaaagtgct gggattatag gccaccattc ctggctgagaattattgttt tcctcgggag 103920 gggtcacatt tccttgcttt ttcatgtttc ttttgtccttacattgatat ctgcacatct 103980 ggtgtaacag tttcttcttt caattttatc gattttcttttataagaaaa aactttttcc 104040 tacagatgta tctataatgt tggttgggta gggtgctttggctttgattc ttggtgggtg 104100 cggtagtata gcttccatat gatttctatg gctatgatcagtgtcagtgg catctttgag 104160 ttcctcagtg attcgggctg tggttaataa aggctgtggtaacgctttgc tgaagatggg 104220 gatgccaggt ggtctagtcc tcaggtatca gtggtagtgtcagtggccca agcatcacag 104280 ctggctggca tacgtgggca ctagtagtgg cagtcatgggtgggccagtc cttggggttc 104340 cacgcagctt gctttggtga caatggtggc agtggtggggtaggcaggtg gatggatcct 104400 caggctactg ggtagtatgc atggcatcag tcatggcagcagtgatggca ggctaaccct 104460 caggcccctg agaagtacgc gtggtgtgcc aatggtggcagtgctggact gggtgggcca 104520 gtccccaagc ccccaagtgg cacatgtggg tgggtgctgctggtgatggt ggctgcaggc 104580 taggtgggcc agttcctggg tccccaagag gtgtgcatggtcaccaagcg gcctatcccc 104640 aggaccccag acagtgcaca caggcagtgg tggtgaatgggctgggactg tctttaggcc 104700 ctggaaaggc atgtgaaagc actggcagtg gtagatggggtgggtcaatc cccaggccac 104760 tggatgatac ttatgggcac tggcagtggc agcagtgggtgaggcagctc tgtcctcagg 104820 cccttggatc gtgcatgtgg gtgcttgtgg tagtaaggagtgtgggttga tccccacgct 104880 cccagatgtc atgtacagat gctggcagag gtaggcaggatgtgtttatc atcaggtcac 104940 ctgatggtgt gtatgggcac cacgccagca ctggcaggtggagtggacct gtccttaggc 105000 cctcagaaaa catcttcagg cataggtggc agtgggtagtgtggacttgt cctcaggccc 105060 caaaacagag cctgggtaag gcagtctcca ggccccctgaaggttcacgc agccttgttg 105120 ctggagggaa tggcattgct gtcaaggtta gtggccctgaacaggtggct ctcaggctct 105180 ggggaacaca tgcttcagct tcctttatcc tggggccagtctccttcatg cactgtactg 105240 cccatgccct agggtttagg acactgcatg ggctaggaggctagggaccc aacagcactg 105300 ctgggttctg ccagtgtcat gatgctacag ccctctcggtggacatggag ggatgtcaac 105360 caaggcttcg ggggcaggct tcgggatatg aagatacaggggctgctggg ccccaggaca 105420 ggatgtaatc tggcatggac tgtgtgctca acatcgtaccatgctgcggc tgcttgggtt 105480 tcagggagtg tgtgagaccc agcatgaact ctccctctggaacaatcctg tcatgtgaac 105540 tccagacagc tacctatact agtctcaggg cccatcagggccaagggaat ttcctgtggc 105600 taggattgca ggagtcaata gtgggaattt ggacctttggagacctctca cttacctttt 105660 ccccacaaag gagacgtctt cctggctcca ggcagatcccagttgggctg gctgcttcat 105720 ttccctctct ttgcatgcct cagagattcc ctgtcatttccctgctgaat tccactgttc 105780 tctcttagga aatctgttca gtgtgtaatt atctgcttagtgttcgggta ctttttggtg 105840 gaggaggcaa gtgccaggct cttctagtca gccatcttgaagcctccttc cagaatagct 105900 ttttagtggt tactctgggc attatatttt atttatatacagtttatcac agtttaccca 105960 tgttgacaac tgactattca aataacgttt aaaaaccatgctttttgttt atatctgctt 106020 aacttcccct gttttaaatg caattgtgtt aaatctttcctctacacacc tttggcggtg 106080 agacaccatc ctgggtaggc ctgacaaaac cagaggagcctttacaagaa gcatcagaga 106140 ggccgggcac atggtggctc atgcctgtaa tcccagaactttgggaggct gaggcaggca 106200 gatcacttga ggccaggagt tcaagaccag cttggccaacatggcaaaac cctgtctcta 106260 ctaaaaatac aaaaaattag tcaggcatag tggcacacgcctgtaatccc agctactcga 106320 gagtctgagg caggagaatc tcttgaacct gggaggcgaaggttgcagtg agccgagatt 106380 gaccactgca ctccagcttg ggtgacagag cgagactccctctcaaaaaa aaaagaaaaa 106440 gaaaacaggg accttcattc tacaactgca aggaagtggattctgtaaag aattgaatga 106500 tttggtagag gactccaagc ctcagataag aatcaccaccctggccacac cttgatttca 106560 acttgttgag gccctgagca gaggaaccag atagcccatacctggactcc tgacccacag 106620 gaagtctgag acaaattttg tgttgtttta agtggctaaatttgtaacaa tttgttatgc 106680 agtaataaaa aactaataca tggttcttgc tttagagctttgttagatgg gactagagca 106740 gcattgagtc tagagctaat tttgccccac tcttcaggcaaaatctttct gtccaatcct 106800 ctgtgagtta tggggttttc cactgtggct attggaaaggaaagtattnn nnnnnnnnnn 106860 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 106920 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt tctctgttataactgaatat cacaacttat 106980 ttcaggatca aaatatagtg tgaggaagaa aagatttatctagtgatcaa acctaaattt 107040 aattttaaca ttttcccctg gggtaaaata gggatgaaaaataagttata aatgtctata 107100 ttctggccat agtttaaata gataatattt tctgtcttcagtgatatttt catctaatat 107160 ccaacatttc ttaaataatt aaagcaaacc tcctagagaataacagaaat tatgaggaaa 107220 gtcctatata taaaataatg aattaactat tgctagttaaatttatgttt atgtttcttt 107280 aaaaaaaaaa aaagatggcc gggcgcagtg gctcacgcctgtaatcccag cactttagga 107340 ggctgaggtg ggcggatcat gaggtcaaga gattgagaccatcctggcca acatggtgaa 107400 accccttctc tacttaaaaa aatacaaaaa attagctgggcgtggtgacg cgcgcctgta 107460 gtcccaacta cacaggaggc tgtggcagga gaattgcttgaacccgggag acggaggttg 107520 cagtgagcca agatggcgcc actgcactcc agcctggtaacacagtgaga ctccgtctca 107580 aaataataat aataataata atagttacag tctatgactagcctgatcat aaatcagtgc 107640 agcacctctc tgggtcttaa cagatgctcc acttatttcgcttatagtca tctctccttt 107700 agcactcagt ctatgttggt gtcccaaggt gctgctcctaaggcacatcc atcagcctaa 107760 actttcatca ttttccctgg tagtgtgagc agaggactactagcacttga gggtttttta 107820 ggctcttaga aatctacagg actcttatct acttcacaacaaaatcaaat gccatgcaag 107880 attgtggctt ttatatcaac catcatggcc gcaaaacatagataatagat atagataatc 107940 tctatatata attgtgtgga cttttacata tactatatatagatagtagt ttggattttt 108000 ttttattatt actaaaagtg gtacctaatt tctccggttaattttgagaa atgttttatg 108060 agtctgagga tgcaatttta aagacaatcg agcaagtgctttctaaaatt gtccacttgg 108120 ctcagatgag tctccatgtt tgagtgcatt ttagactagttcattgaaca tgatagaaaa 108180 tgtcaaggat tcaaaatctc ccaaacactt gaagtttgtaagttattctt gtgagtatcc 108240 acttgagaac atttgcaggg aagagcaagc atcaaaggaattgtagtttg gttaaaaaca 108300 aataaagaaa cagattgagt ccagaaagaa ctggggcttgagccctgcag tgtcacatac 108360 tggttatgtg ggtttgggta gtttcttaac atcactgacctcagtttcct cagctgtaaa 108420 acaagagagc aacacctact tgatagggtt gatgtgaagactaaataagt taaatgggaa 108480 agcacagaaa gtgctcagct cagccactga cacataaaatatattcaata aatggtaacc 108540 aatgttatta aaaatatatt aaggaagcaa tatctaaggcatgactccta tttataatgt 108600 tggctaccgt aatctggaca gttgttccct atctcttctgtccaatcttg gaaaaaatat 108660 ttttggaata agaaatgaaa aaatttttaa tagttttacacaaactatat tagaaaacaa 108720 attaacttga atttagaagc aataacttct gtgccttaaaaagggtgcct aaatattatt 108780 ttcagatagt tatatgtgtt tataatgttc ctcccctgtggtaaactgag gatggaggaa 108840 taaattgaat caaaatacat ggtgaaaaat aactggattcagaagaccct gagagacaaa 108900 aaaaaaaaaa aaaaaaaaga agcagatgat aggaacacttcattccaatc ccttaaaatg 108960 tgattctcag tttctctgtt agatccaatg acaaaagaagcatattatct aggctcaggc 109020 tggctggaga ttgatctgct gaatggagct aaaaacaaatttccaagaaa atttaataga 109080 ttgaccttcc ctataccata ccaataaatt ttatgggagaaattacccct ctttaatgaa 109140 aggaataaga tgttgctcca tttctaactt aatccgtgtaaatcacccgg gaagggtagg 109200 cttgtccagt aaatgttctc caataaaatg aattgcaaagaggctagact atttaataac 109260 aagatgaaat tttcagttat ttgaggaagc tgcattcactatataacaaa aagcgttatt 109320 accacaggag aaatactttc aagattcact gttctttcactaatataaat aactcctttt 109380 gggcagttcg gattatttag aatccaatag aaaaagaacattgctggtaa tatctgctgt 109440 aatagaaagc aatatcaaat cctcttttct ggaagcataggattgtgatg gctagacaat 109500 gttcctacaa ttcgttgctt aaactgtttc atggttctttttactatatg aactttctta 109560 gattagttca aatttgagga gtttttacct gaagttgttccttaagggtt ctatgtaaaa 109620 tagatttaga atggagttgg cctctttccc agagctgagggcttatagtg gcttgggcag 109680 cttgggcaaa gagaccatta agaaaattct tgtggtaggagagaggcagt ggcttggacc 109740 aaggtggtgc cagtggagat aagagtagtg gacagattatatatatatac acacatttat 109800 atatatttat atatatatat ttatatattt gtatatatatattttatttt tatttttatt 109860 ttttttttga gacagagtct cgctctgtct cccaggctggagtgcagtgg tgcgatcttg 109920 gctcactgca acttctgcct cctgggctca agtaattctcctgcctcagc ttcccgagta 109980 gctgagatta caggcaccca ccaccacgcc cagctaatttttgtattttt attagagacg 110040 gggtttcacc atgttggcca ggctggtctc gaactcctgacctcgtgatc tgcctgcctc 110100 ggcctcccaa agtgctggga ttacaagcat gagccactgcgcccgaccta gagatatatt 110160 ttgaaggaag aaccagtaga tttatttgct aatgatttggttatagatta tgaagggaag 110220 ggaaaaatta ggaataccac aaagtttgag gctataataattgaatggga gtggagccat 110280 ttacagagct ggtaaactgg gagagtgtta gatttgagaaggaagataaa aggtaacatg 110340 atatgagtct tctgtgtata agaaacatct aaaaaaattaaaataattaa agcactatca 110400 agcctttgct tttgaaaaca tgttctaaat ttttttccatgatatagtat tgttttggtg 110460 taatcttcag acctctttta aactatttaa cactttgatagctctacaga taatcataat 110520 agatcataaa atatttacta ctcatttgta ttgcaaagggttagtttatc ttttatgaag 110580 attaaaagac aaatttttca gccctctctc caagttccattattttaccc cccaaaacac 110640 aattaattac atgcttttga aaatcaatat atttgtttcaatagcagtac taactgggat 110700 ggcagggcta agccaattta tttaatggag tagtttttgttttgggattg agtgattcct 110760 tgttgaacct gcatacatat aaagacttgg tttcattaaaacaaacaaca aacaaacaac 110820 ttcaggacag aaatgctttt atttttaact ctgagatttaaccaggaaaa cgacaatttt 110880 aaaagctgag tttagacgta gaattaatag caactcatttgctcaaacaa aagataagag 110940 tgccatctag tgttcattag gagtatctgt aacttggactactattttct caattacagc 111000 agaaaaacaa atgaggggag acaaaggaaa aagaccactgtgatagccac agcaattgaa 111060 aatagggaca tgattcctga gagctcagag aggggcaccagatctgaatt tagaaattaa 111120 gaagttgcat catgaagtag ctacctcctg agctgataaaggaggaacag tcacaggtga 111180 caagggcagg aagagcactg atgtctaaga catgagtcaaggtgggaaat caaagaacaa 111240 cataattttg tgggcaagtt taactgcttg agataaagcagtggaaagtg agtgcctgga 111300 aggaaggcta gagacgggag caaagctgag atcaaagagagtttgtattt tctatgcaat 111360 gggaagccac tcaagagaca gcctttcatg gtggcccacccctgtagtac tagctactcg 111420 gaaggctgag gcaggaggat ggcttgagcc cagaagcttgaggctgcagt aagctatggt 111480 catgtcactg cacttcagct tgggtgacag agcaagaccctgtctctaaa aaaaaaaata 111540 aagataaaga tagaaaacag agaaacagcc ttgcaattcagatacaaccc tggcaccata 111600 tgggggatgt attagggtgg gaattgagac taaaattatgatgtaattat agtagatgat 111660 ggtagtagta agcaggaaaa aaggatgact caaataattggaagaaaaga taaataatta 111720 ggtttgatgg ctggatgtca tcttttagag ttcagatagattatattttt atcaattaac 111780 attttaaaat tctatttaaa agagtaaagt tagtgggaaagatgattaat ttcatgttga 111840 caagttaacc ttgagctgca tgggaaatag tgatgtgttgatgctaacag gcaattgaat 111900 atctggttct taaagctttg aggagaaggt taagtagaagatataaattc attttcatca 111960 gcacagtggt ggttgctgaa acaatgtgaa caagatcactcaggaaatat atatagtata 112020 aaataagagc ctggatagaa aagacattac taatgcttagtttaaaagaa tgatttgaat 112080 ttggatatgt tcagaatata ttagaaagaa aggagataatttctttaatc tcaaattata 112140 attcactgat gaggatcagt gtttcagaag ttccctgttgaggtctatct atacaaatat 112200 tcaatgaata cattttctat ttatgaacta tattaatcaggaccctttca gcaaaggata 112260 ggagctaaag ttgcaaaata gttcgaagag ttgctcatatccttcatcca gcttccccta 112320 atgttaactg acatattata taattttagt acaataacttgacaattttt tatcccagaa 112380 aagatggtat cctttctaaa acattgtgac ataaattcctggtcaattaa gatgagatgt 112440 gactattata ttcaatagta tttccttaga attaaagaacaaatttaaat ctggaagttc 112500 ttaaagtcaa ggacatttga catctttgct cttttccagttgtattttag ataaaacgtt 112560 tgcttgtcta gataataaat tgggctgttt ttcctcccctagagtactga tgaaaagtac 112620 cacaataatt attgaataat atatgggaaa tgtcaacagagaaatggaaa ctataaaaaa 112680 agagccaaac ggatattcaa caactgaaaa gtacaatttctgaaataaaa acattcactg 112740 gctgggctta atagtgattg gggatggcag aagaaagggtcagtgtgtta atctgagttt 112800 tctgagaagt agacaccaag aagaaaccaa acatgcaaggatttcttttt attgcaatag 112860 tttttgggga acaggtggtt ttcttgttac atggataagttctatagtgg taatttctga 112920 gattttggtg cacctgtcac ccaagcacat acgctgtaccaatgtacagt cttttatccc 112980 tcaccccctc tctcccttct taccaagcct ccaaagtccattatataatt cttatgcatt 113040 tgtgtcctca tagtttagct cctacttata agtgagaacatacaatattt gtttttccgt 113100 tcctgatttt ttttacttag aataatggtc tccaaataattnnnnnnnnn nnnnnnnnnn 113160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 113220 nnnnnnnnnn nnnnnnnnnn nattacaggc atatgccaccacgcctggct aattttctat 113280 ttttagtaga gatgggattt taccatgttg gtcaggctggtctggaactc ctgacctcag 113340 gtgatctgcc cgtctcaggc ccccaaaatg ctggggttacaggcatgagc caccacgccc 113400 agcccccgac cctttttttt ttaaatatct attaacattttgtagaacct ctttgggaaa 113460 ctgctctcta gactgatgcc ctcattttta tagaaagataggatacaggg ataaaccgat 113520 ttgcttaacc aaacagtccc ttatttactt agacaaactcacaggtaagt aagctacaca 113580 gtattgttat ttttattact tatttattta tttattttgagacagggtct ctctctgttg 113640 cccagactgg agtgcagtgg cgtgatctcg gctcacggcaacctttgcct cccaggctca 113700 agcaattctt ctgcctcagc ctccggaata gctgggattacaggcgtgca ctaccacgcc 113760 gagctaattt ttgtattttt agtagagatg gggtttcaccatgttggcca ggctggtatc 113820 gaactcctaa cctcaaatga tccacctgcc ttggcctcccaaagtgttgg gattacaggt 113880 gtgagccact gcccctggcc tttttttttt tttgagacagagtctcagtc tgtcacccag 113940 gctggaatgc agtggcacaa tcatggcaca ctgcagcatctacctcctgg gctcaagtgg 114000 tcctcccacc tcagcttcct gagtagctgg gactacaggtacacgccacc acacccagct 114060 aattttttgc atattttgta gagatagggt tatgccatgttacccagact ggtcttgaac 114120 acctgggctc taaatatcct cctgcctcag cctcgcaaagtgctgggatt acaggtgtaa 114180 gccaccttga ctcttaaatc agattatgta aatcaaataactcagcatct gatgtaaatt 114240 gaactatttt ggggaaggga gtaaattaac tgttaatgacctagctatga aaatatcacc 114300 ataaacggcc cttacaaagt tcagcagagt ttccactaccctacagaatc ttctctgata 114360 actcccattc agagtgattt tgcccttctc tgatcaatatgtcatcaaat tctgtcttct 114420 attgttacct acctgcctcg tgtgtaatct tatcttcctgagcagattgc aagttcctag 114480 aaagctagaa ggaattctta cttttcctta gagtcacttgagtcacataa tagtctcaat 114540 aaataatttt gaaaagctca caaatgacca actgaatggaatagggtcag catgaaggat 114600 tacagaagga gccagccatc cacatccttt ctatcttaagtgcaacactc tcccttcctt 114660 ctcattgtat ggctagcaat tacctcttgg caattttttctagaggtcta cagtaaagga 114720 ggctgccatt gctcaaggac gctgcttgat cctaccaaaattcccatgga tctgtgagga 114780 gctcctcaaa tcagcaggac atgcccctgt cctgcccaaaatctgtttgg agatcaaatg 114840 ggccttactc aataataaaa tggaagcctg aatttatttctatttgtttg gcctctgatt 114900 aattaggtac attctccaga atacaaagca gctgcttttttgaccattaa actgtgttcc 114960 ttaaagagaa gcgcatcccc gagtaggaac agttctaatgatttgtttta gtttgtttta 115020 tggcaagacc ttagagaagt cgaagaggaa gaaaggagactccctatccc tccttattgc 115080 tgacgatcca ggctgacagt agttggagag caagctccgtgctgaaaaga ttcaaacaca 115140 cccatgaccc acaaactgca aactgtcaga ttaattgtttttgcctacag acaactcaat 115200 gagtgctcat tgtgcagaca gcatgttctc agcactgctttaggcgccat gggattatag 115260 aagaggtata aaggtatgtt tcctcccttc caggaatttacaatctaata agatgagata 115320 agactaacat tgaaagcagc tgtggacaat acaaggcaggctataggatc tagtgctgac 115380 tttggggcct tggactttta aatgtaacag aaattcagagagggaggaaa agtcactggg 115440 cgtttcagtc ttacaagaag acttcatgaa gaaaatacaacaggagttga acattaaagg 115500 aaaggcagca ttccaggcaa aggaggcagg ctgtccttttcagtttcaga caatattgtg 115560 ttaatcattt tacatgcccc aattcttgat tgctacaaggcgaaaaggta tataagcacc 115620 catactttca atcactgggc tattgctttt taaaaattacctttaagata tggttgtctt 115680 agtggcctat aaataaattt gctcaatttt ccattttacccatttggcat gctcagatca 115740 ataatttgac ttattagctt tagagttatt aattacacggatgcagttta ttaacaaaaa 115800 gaaaacaaag tgcttactat gtgctaggcc ctgtgttaggtgccggggat aaaacagtgc 115860 ataaataatc aagatgcctg tcgagtttaa aagcaatagcaggccgggcc cggtggctca 115920 cgcctgtaat cccagcactt taggaggccg aggcgggcagatcacctgag gtcaggagtt 115980 cgagaccagc ctggccaaca gtgagaaacc ccgtctctactaaaaataca aaaattagcc 116040 gggtgtggtg gcgggcgctt ttaatcccag ctactcaggaggctgaggca ggagaatcgc 116100 ttgaacctgg gaagcagagg ttgcagtgag ctgagatcgcaccattgcac tccagcctgg 116160 gtgacagaat gaaactctgt ctcaaaaaaa aaaaaaaaaaagtgacagca gacacagaca 116220 tacaaagaac aacatgatta taattacagt atgagtcatcagagaaaaac atagagctag 116280 aaaaacagat atcagataag tctaatttat attggaaaggcagtgacaaa tttcttgagg 116340 aattgataat ttaggctgag acctgaagta tgtaggagttacaggcaggg gaacaggaac 116400 agaaaggcag ctaatatgct ctgaagagca gagcgagatgaggctagtca agtaagtaga 116460 gctgatcata cttatctatt tatattacaa ataaattataaattatagca agctgaacta 116520 ttcagcagtg atgacattta atttttatcc ttttatcacaagctaaaatg ataattaatt 116580 tctctttaca ataaaaacaa tttgagaaga tgaactcgaaacagaaatgc cttgttgtat 116640 ttcattccat gttgtctttc acacagtagc tagctacaagatacaaatga aatgttaaaa 116700 ttccagctaa agtggttacc tctaaaatta tgaaataaactgagagaaat caatccccca 116760 ggaggcaaca tagtgaagta ggtaggtgca tggaattttggaaccagact tcctatcttg 116820 aactcctggg ctcaagtaat cctcccacct cagcctttcaagtagctggg actataggct 116880 accagcaact atagaaacca gattccctgg gttgaatctcagctcaaaaa acaccatcag 116940 tcaactggat atgactgaca tctatagact acttcctccagcaataacag aatacacatt 117000 cttctcaagt tcatgtggag aaagccttca ccaaggtagatcatattctg ggccataaaa 117060 cacaccttaa caattttttt tttttttgnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 117120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 117180 nnnnnnnnaa atggaagaat agcaggttca ggagaggagaaagaagaaac gaatacacag 117240 tgcatgtcaa caggataaca tatagaggat ttctaaaagccacctccaag acaaattcag 117300 gtcccaggag gaacagactg tacaatcctg agtttcaagaaggatgaaca gatacatcaa 117360 aggtgatgca tgatttggag gcataaaagt aagcaatgtcttcacaatac tacacatcca 117420 gagaaacaca aatgcagcgt catctgtgga cacggtcctcctgttaactc catacaagat 117480 cagccctcca tctgcaggtg tcatagcttc ctcaacaattagttcccaag tcctataaaa 117540 tacccaacca aaaaattgta gatctttcat cttttagggaaatttttaat tgtaaattta 117600 ggattccacc atttatttaa agcaaagtca ctagtagctgagggtggtgg cacacgtggg 117660 tggtaccagc tactcaggaa gtggaggcgg gaggatcacttgagcagccc agaagtagga 117720 ggctgcagtg agctatgatc acaccactcc attctggcctgggtgacaga ctgagacccc 117780 atgtctttaa aaaaaaaaaa aaaaaaaaag caaaaagcaaagccactata ttagtgtaag 117840 gagggcgtgc ctgcttcctt acagatgata tcacatgtgttcttgtttac ttatttattt 117900 aacttaattt ttttttagat agaaccttgc cctgtcacccaggttggagt gtagtggcac 117960 aatctcagct cactgcaacc tctgcctctt gggttcaagtgattctcctg cctcagcctc 118020 cccagtagct gagactacag gcactcacca gcatgcttggctaatttttg tagttttggt 118080 aaagatgtgg tttcatcgcg ttggccaggc tggtctcgagctcctgagct caagtgatgt 118140 gcctgcctcg gcctcccaaa gtgctgggat tacagacattagccactgcg tcctgccaat 118200 atcacacatg ttctgagatg aaaaccagaa tggctttcttgtctgggttc ctgactgctc 118260 cttagctttg agttcttcta gtttcttctc acagagactcatgaagtaat catctggttc 118320 aatccctgca ttcttcaaat tttccaggaa tttttccagtgtgtccactg atctggcttc 118380 ctcaatcttt cttgtgctca ggtagagtga actcctagaaatccaggtgc ttacaagtgt 118440 ccacagagga gatatttctg acatcactaa ttctatccaaacaaggaaaa aacatcagtt 118500 ctgacaactt ctctaggtcc cagaggctca cttggaatccaattaactag gactagaagc 118560 cagtgcctcc actgggtatc acaaaggccc ctagtggcattagttctgta gaaactgggc 118620 ttctacaggc caggatcacc tcataaaggt gtgaggggactgccatcttg tctcaccgat 118680 catctggtac ctcttttctt tccatcactt ctagtctgcggtaaggtcaa aggccaagat 118740 actacccaaa tatcttcaaa cctccctgtc agttctcaacagaacatttc tattctgttt 118800 caacatccag aattattatg aaatcccaag gcacaatagtagaaaggtaa aaggtttcag 118860 ctttacttga acatttgtta tttccagctg gagccgtgtgtcctcaggac tacctgcttc 118920 caacatggtc ctcgctgaag gcagtgaagg ttggagggatattgggacca ggcttaaata 118980 tacaatgttt tctgtctgca tcaccttgtc tctgttccagttaaagggaa tccaaattgt 119040 ccccagagcc ttttctgcag attcatccag ccccctcactgccaccactg cctcagcacc 119100 ctgactccag aggaactgca gggctgtgag ccgactcccatggtgcccac tatagccctg 119160 accgtgcagc cactccaaaa acaccaggcg cctctgaggtgggaagtaat accctcggca 119220 gttatctgac ccaaagtacc acatatttgc tttatttatttatttattta tttttgagac 119280 agagtctctg tctgtcgccc aggctggagt gcagtggcaagatctcggct cactgcaacc 119340 tctacgtccc aggttcaagc aattctcctg cctcagcctcctgagtagct gggattacag 119400 gtgccagcca ccatacctgg ataatttttg tattttttagtagagacagg gtttcaccat 119460 gttggccagg ctggtttcta actcctgacc tcaggtgatctgcccgctca gcctcccaaa 119520 gtgctgggat tacaggtgtg agtcatcttg cccagccacatgtttgcttt aaatggaatg 119580 tctggggact gttaaacatg tgcctaggca tacttccacattcgaatggg ttccaagagt 119640 cctgtggaaa aggaactgaa ctctcttcat gattggacacatttgatgag caggtgtggg 119700 gatgggtgga gggagggaca tgaccagagc tggataaggaaagtgaaagc ccttagggga 119760 atcctcctga gcgcagaagt ctggagagtg ggccagactgcatcaatctg agtgttggga 119820 acggtgattc tgagtaaaga gtgaggctat tctggaactacctagagtaa ctgtggtaga 119880 gatgaaagaa ctgaataaac agaccaagag acccatttggggtcctcttc tctaaaaacc 119940 tgagagtgag aagtgggtta tcgacagcac ctgtaatgtcaacaattctg agctcccatt 120000 gcaaagttct cttcctcaat tattgatgag aatcccttgggtgaactcca agtatcctat 120060 agaatccact agttagtggg tcttaaataa gatctctttccattctcttt ctaaacttca 120120 ataaaatctc gatttcagaa tataatcatt aaaaataaaatcaaatattt gaagaggcca 120180 ggaaaccaga agtgtaaaat aaggagtctg tgtgacttcaggtgctctga taaggaagtg 120240 ctaggcgagt aatattagag atgcaagagg tagattgggagaaatgccca ggagggacaa 120300 ggggaacagg ttgggcagga agggccttca gattttggagctgatctgac acctgtgaaa 120360 ggcaagtggg gaaagaagaa agcttgacta gaaagggactccgttctaag aaagtttcac 120420 cagaccagtg ggaaaccctc aagccaaagc cacccattacaggagcaccc tgaaccagca 120480 ctcacaacca tacccagtcc ttcacaggaa agcagcctggggagcacggt gttgttgcaa 120540 cctggaaggg ggctggggag cggcagctga ggctccctgccaactatttt tccctgcatt 120600 aggaaatcca agtggtgcat tttcatccct gctccatagagttcattcaa taatgtacta 120660 taatctgtaa taaggatact ccggccacgt ggagttgaggttcagcagat ctcccacatg 120720 ctaagtggca tttccctttc tgatgcctct ccagaactttggagcaagga gagtattaaa 120780 tgtaatccaa tctgctggct gaagctctgt agtcactctgcttcatggcc cactaacctt 120840 ggccctgttg atctctgcct tctccaaaaa ttaccttttcaggccaggca cagcagctca 120900 cgcccagcac tttgggaggc tgaggcaggt ggatcacttgaggtcaggag ttcaagacca 120960 gcctgaccaa catggtgaaa ccccatctgt actaaaaatacaaaaaatta gccgggtgtg 121020 gtagtgcctt cctgtaattc cagccacttt ggaggctgaggcaggagaat cacttgaact 121080 ccagaggcag aggttgcagt gagctgagat cgtgccactgcactccagcc tgggcaacaa 121140 gagtgaaatt ccatcttgaa aaaaaaaaat taccttttcatcccttcaag cctgttctca 121200 gtgctggcca tctgccatgg cctagcacct cctggaagtccaggatcagc tgcccaggaa 121260 gcagctcccc aacaaagtat gaactgtgtc tgcctctgcccacctctgga tatcaggtgc 121320 ctgcttgccc ttccaggggt aattgctgtc tccttataacaggtaaccac tgttttcagt 121380 gtcttattgt ccttccagta tatcaatggc atacgactgatattcacctg ttttcattct 121440 tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 121500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnntataaaa aagaccccag 121560 agagctccat tgccccctct accatgtgag gatacagctagaaggcatca tctgtgaatc 121620 aagaaatgga cccttagcag ataccaaatc tgttggtgccttgatcttgg acttccaagc 121680 gtctagaatt gtaagaaata aatatttgtt gttgataagccaaccagttt gtggtatttt 121740 tgttatagca accaaaatgc actaagacag tatttacatatcttattcta aaatatttta 121800 tgatggctaa aatgtttatt atacattgtc atctaaaacagtaagttttt ctttcatttt 121860 tttttaatca catgcacaaa tgcattaaag taaagatccagtggccttac catggtctgg 121920 aggcccatgc ttacatctcc acctgtcctg tccctccagccgatggccct agccttcttt 121980 ctcagttcct cccgtgtgtc aagcacttcc tcttagcaccttcaaacatt ctgaagtgct 122040 ctctccccta ctctccattg tgctttgtct caatcttcctcttcatctca acacagaaaa 122100 cctcttcaaa aactttctta aatctccaag acaaattatgaactcccagt tagtctaacc 122160 ttttctagcc ctcatcccaa cgaataaaat catttaattatataattaca gattagtgtg 122220 tgtattagtt atcttctgtt tgcgtaggaa ctcactgaatgttggttgaa ttaatgaata 122280 gacaattaaa atagttaagt atgacaaata ttattaataaaataaaaatc tcatttattc 122340 ttcacagatg tgaaatataa ggccactcaa actgaacagcatacttaata tatttatgaa 122400 aaaaatttaa gatcttcacc tcataatcac ttgaatagaactagatgtga caaaatatta 122460 ctacatatgt taattatttg tgagcagcta gttcaaattatcattatcat ttatgctaca 122520 aacttttgtt ttttgttttt tttgagatgg agtctcattctgtcacccag gctggagtgc 122580 agcggcacaa tctcagctca ctgcaacctc cgcctcccgggttcaagcga ttctccctgc 122640 ctcagcctcc ccagtagctg ggattacagg cacccgcaaccacatcgagt taatttttgt 122700 atttttagta aagatggggt ttcgccatgt tggccatgctggtctcaaac tcctgacctc 122760 aggtgatcca cccgcctcag cctcccaaag tactgggattacaggcatga gccactgtgc 122820 ccagcctgcc agagaaaaac atttttataa aaattatataaataaaaatt ttcatgaatt 122880 taaaactagc tttttctctt tttagtctga agttttactcttgtcaatac atgcatttat 122940 ctatttggac attaattcaa ttacatcttt ttttattatcttcttggcat taggccctgg 123000 tgattaaaag atgaactgta catagtcttc accctctgagttttgagcca agtgagatgt 123060 atgccaacca gttattacag ctaaggggta gcccagcacagaagtaaatg cagtgagctg 123120 cttattgtct ggagaggtca aggaaggtgt tttggaaataacgtgcttga ggctgcctca 123180 tattttaacc gttgataaag aggaaaagta ggtgaaatatctttaagaaa tatcaaaaaa 123240 ttataagtcc atttataact tcttatgaaa gccattttttacatttgtaa aaaaatacct 123300 ttatccattt tgtcagctgt cttcagtgac aactagatttcactcaactg cagtgtggca 123360 gaggctggct agatgcacag caaacccact tcctctccctggagacaagg tcgaacattt 123420 cctagctctg acaatggaac gtggctgatg tgaaggacaagacagttaag agttgtttgc 123480 cttccctata tgctctcttc cttccacagc aatcttgaagtctagaattg gaagatagta 123540 gtggaacaag atgaaaggac ctggatccct gagtgacggcaaggagaagt gtctcaccgt 123600 gtcagactac agcatgagca agtaattaag ccagtggaattttaaggatg tgtgctacag 123660 cagttagctt atcctaatga atcattccat tgtgagggtagtgtgtagtc aatcataatt 123720 ttaaagaaca taatgaatga cagatattct tagtcctgtttttagtatct acccccactt 123780 ctttgctaac aggactccag ttttgtttgg ggtgccaatttgcacagctt taagggaaga 123840 ttttggttgg tctaaactca tcatgagaat tctgttccccacgcttttag cctcccctgt 123900 agtgagggat ggtcatgcag cccagttcta gctaagaaacctaatggaaa gtccaccgca 123960 gggatttctg ggaaaggttt tccttccctg ataaaagaaacctgcatggc tggcatcatc 124020 cctttttccc ctctacttaa cttaaacagc aacctgcatgagtgggggct acattctgga 124080 ctaacataat aaatgcattc tttttttttt tttttttttttgagacaggg tcttgctgtc 124140 acacaggcta gaatgcagtg gcaagactat agctcactgcagcctcaaac tcctgggccc 124200 aaggtatcct cccacctcag cctcccaagt acctgggactacaggtgcat gctaccaagc 124260 ctggctattt ttttttttta gctttttata gagacagagtctcactgtat tgcctagggt 124320 ggtatcaaat tcctggcctc aagtggtcct cctgccttggcctttcaacg cgctgggatt 124380 acaggtgaga gccaccatgt ccagtcaata aattaattctgaactaacat gacaatacac 124440 taatgccata aatttgtggg gtgatatggg acatgcatgaaaaaaaagtg catgccccat 124500 ctaaagacat ccaaagatat ttttatttaa atattaaaatgacaaaacaa aggcctcaat 124560 gggcccaaaa ccactcagtt catgaaattt gaaatataaaggtctgttat atactgttaa 124620 ataacagtca cacttcattg tttgtcatca tctattgaatacagaacatg tgtctctcat 124680 actttcttaa tgtgtttcct ttaaatcaat atgatgtactggctaatagt tcaggctttg 124740 aaatcaggct gcctggattc aaatcccagc tctggcacttactgtgtgaa ctcaagtgta 124800 gtgtactaga cttctacctg tttttttatt actaagatggagataatcat agcatatatg 124860 tctaaggatg gtgataagga tgaaatgaca caatcaatacaatgtactcg gttgagtaca 124920 caatgctcaa taaatacaag cagttatttt tattacttccaaatgtcacc agagagcttc 124980 aagtagtgaa ataacccatt gagaaagtcc ttgctgactatgcgtgtaag tgtactttat 125040 gttcagggac tactgatgag taaactaaag aataattaaggttgcctggc agcctgtgga 125100 ctccttggat tttaggcagg gttaaaacaa catcacagcttagttgtttt aagtaaaaga 125160 ttagtcagtg actaagagtc aagggcggga tgagagaggacactcgaaga gtgggtgtgt 125220 gccctctcct atccctatga actcttacac cctgggcttatagtatctgg ggcctggctc 125280 tagacatgat gagaaagaga taaaagccaa ggaatagccacagatgataa tcctaacagc 125340 gcaggcagcc aggcttccct cctaccactc catccaccttccccatgatt ctccaaaatg 125400 attatttgcc ctaacaagga ttaaggccca tgtttttctgcctctacatc tctgctcatg 125460 caactccctt ccctaaaaaa cccatcagcc catctctgcctgtgaaacac tatttgtttc 125520 tcagagttaa gcataaatgt cccctttttc actttgttgaacaagtgaca cttctcagat 125580 acatcacaag atagcatgtt acactgttaa gagcactagctcatagacag gttttcatca 125640 atctccaaag taccaaacga tatgttcaat atattttcctaaatgcatgt acatacacag 125700 aattatctca ggaagggtat actagaaatg gagttatctctggttagggg atttgggtga 125760 ctgggaaaca ggtgtagaat gcaacctaat tttcattattagactcttta atatatttaa 125820 cagtaaagta aaatatgttt taagttcttt cttgttgtatgttttatctt cctctgtgaa 125880 atttttatcc taatcctacc tatttctgat ctctagagatgcccggtata aatcaagtat 125940 gctgactaat taattgataa acatgttaat gaactgttgctaagaatcat ataaatatgt 126000 atatacaact gagtttttac taaagccctg aaagtattaataacatattc ttttaagtga 126060 gttaaaaatt tgttccagta tgcttaaaca atcctttttttcttactatt acaccatttc 126120 caaatcagtt gtcttcatta gtttatttcc tcttctgactctcatgtctt gttttaatta 126180 attttttcca taggccttgt attttcattc ctactggcagaaagcacttg ttgcctaagg 126240 cattaagttg ttatcttgta tactacatga gcagctctacaatgttcttg acacctgtag 126300 tgccctctta ttgcatgaag ccgtaagaca tgttcagtagctttaaggat acataaagaa 126360 ttcccagaat tgggcataat ccccaaatta taaaacatctttgagaagaa aggccattcc 126420 taaatattgt aaaaaaaatt atggaatgaa taaagatatcctcctttacc attatgaata 126480 ggtgattata ctgggaatca aacagcctat gctctaatcctggctcttta agtatctctg 126540 gggccccaat ttcttctcca tcagtaaaac agacatgagtattagactaa ttttgagatc 126600 gctttgcact ctagttttct gtttatgaca tcttcatcccagaaagtacc caggcattac 126660 agtacacgta ttaataatta gcatttataa ctactcacctaatgaaaagt aacccttttc 126720 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 126780 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnaattaaaac tgtaaacttt 126840 tttttttttg agacggagtc tcactctgga gcgcaatggtgcgatctcag cccactgcaa 126900 cctctgactc ccaggttcaa gtgattctcc tgcctcagcttcccaagtag ctgggattac 126960 aggtgcccgc caccacactt agctaatttt tgtatgttttaatagagacg ggttttcacc 127020 atgttggtca ggctggtctc gagctcctga catcaagtgatccacccgcc tcagcctccc 127080 gaagtgctgg gattacaggc gtaagccacc atgccccgccataaaatgtt aattttgaaa 127140 acactgcaag acagtggatt caggaagcct catgggaaataatcatgtta tatacccatt 127200 tacagatgag gaaactagga cacagcaaca ctaggttactcgctcaaggt cagatcggta 127260 aaaagtgtca aattcaggct attaccaacg tgttctgaactttaaatttg ggttattccc 127320 tgtggtacca tgctgcctgc atcttttcaa aacatgatttgtactgggga gattataaag 127380 cagttagctg ggtgtggtgg ggcgtgcttg tagtctcagttactccggag gctgaggtgg 127440 gaggatcact tgagcccagg agttcaaggc tgcagtgagctatgatagtg ccactgcact 127500 gcagcctggg tgacagaatg aaaccctgtc tcaaaaaataaaaataaaca aagaaggaga 127560 tgtgaactcc atcccactcc tcaccccgta tctgctgatctagagtctaa tgaccaccta 127620 acttaataaa gtcaggtgcc agagtaaatg agcagtccacagatttgggt ttcacagacg 127680 tttcttcctg atcccatgga ctgaatctgg cctcttttcctcctttgttg gtaatggcac 127740 tcctcccagg catttagaag cgagcaattg ggccttaaaggagcgagttg gtcttgaaaa 127800 aagctgttct tcctgacctt tcaattggca aaattcatactagctttcat agaccatagg 127860 ctgatttagt gagtactgac tgtgacaagt tattgcactattttacacat tttgtcttgt 127920 tttgttttat ccctacaaaa actcactgaa acgtgtagcattcttatcat gtcattttac 127980 agatgaagaa acaccttaag ggaggttaag taatttgctcaagatcatga ggtagcaagt 128040 gttgaatgca gaatttgatc caggggggaa gactccagagcccaggctat tattctcttg 128100 cctttgaaca taaagatagt tgaggccatt ctgtgctgaactcccgcctc tgcaccttta 128160 atcatcaggc cccagcagtt aagatctctt caaaggttctgcatggtttc agactaactg 128220 ggtaacaaaa caagtatcag aagtacaata tccattctgaatctaaaata cagtctctaa 128280 gatttaaagt gttcatggat tagaagactg aatatttttgccgattctcc caaaattggt 128340 ctacagattc agtgcaatct caatcaaaat tctagcagacttctttgtat atatgaaaag 128400 ctgattctaa aattgactgt gatttataat catcaaaacaatattttaaa aattggagga 128460 ttcacacaac ctgatttcaa cacttaatct aaagctacggtaaccaagag gatgtagaat 128520 tggtgaaaaa atagggacgt agataaatga aacaaaatggagaatccaga aatagactca 128580 caaatagatg gtcaactgac ttttaacaaa ggagcaaaagtattcagtag acaaagaata 128640 ccctttttaa caaatgaagc caaaacaatt ggacactcaaaagcataaaa aatcaacctt 128700 aatccacatc ctgcaagata tacaaatatc aaattaaaatctgtcatagg gctgggcgct 128760 gtggcttaca cctataatcc cagcactttg ggaggccaaagcgagtgtat cacttgagat 128820 caggagttca agaccagcct ggctaacatg gtgaaaacccacctctacta aaaatacaaa 128880 aattagccag ctgtggggcc acacacctgt aatcccagctactcgggagg ctgaggcagg 128940 agaatagctc gaatccagga ggcagaggtt acagtgagccgagattgcac tgctgtactc 129000 cagcctgggt gacagagtga gatttcattt caaaaaaacaaaaaataaaa ataaaataaa 129060 atagatcata gaccatttaa ataaaaccat agaccatatagcaagatcta aaaatataaa 129120 gtttctggaa gaagaaaaca tagaagaaaa ttattgcgaccttgagttac acaaagattt 129180 tttagatagg acacacaaaa aaatatgagc cacaacagaaaaaaaaatca ataaattgga 129240 tttaataaaa tttttttaaa cttttactct ttgacaaacactattaggaa attaaaagac 129300 aattacctac tgggtacaat atctataaat cacatatatctagaatatat aaagaattct 129360 catatatcaa taataagaaa acaagcaatc tggccaggcacagtagttta tgcctgtaat 129420 cccagcactc tagaaggcca aggcagttgg attgcttgagcccaggagtt cgagaaaagc 129480 ctgggcaaca tggtgaaacc ctatctctac aaaaaaaatacaaaaattag ccagtcatgg 129540 tggtgtgcgc ctgtagcccc ggctactcag gaggctgaagtgggaggatc gattgagccc 129600 aggagttgga ggctgcagtg agccatgatc atgccactgcactctagcct gggcaacagc 129660 acaagactct gtctcaaaaa aaagaaaaaa aaaaggaagaaaaaacaagc aatccaatta 129720 aaatgggaaa aataattgaa tagacccttc accaaaaaagatatatgaac aagatataag 129780 catataaaaa gttgtgcaac atcattaggc attaaggaaaatcaaattaa agctataatg 129840 agatagcact ccatacctat tataatggca aaaaaaaaagaaaaagaaaa agaaaaagaa 129900 aaaaccccaa aaaacatgac aatactaaat gctggccaggatgcagagca actggaactc 129960 tcatgcattg ctggtgggaa tacaaaatgt acagccattttagaaaataa tttcatgctt 130020 ttttataaag ttaaacatgt atatgccggg tgcagtggctcacgccggta atcccagcac 130080 tttgggaggc cgaggcgggc agatcacgaa gtcaggagattgagaccatc ctggctaaca 130140 cggtgaaacc ccgtctctac taaatataca aaaaattagccgagcgtggt ggcaggcgcc 130200 tgtagtccca gctacttggg aggctgaggc aggagaagggcgagaacccg ggaggcggag 130260 cttgcagtga gctgagattg cagccactgc actccagcctgggcgacaga gtgagactct 130320 gtctcaaaac aaaacaaaaa tatatttacc ctatgaaccaccaatcccaa ttcttaggta 130380 tgtacccaag ggaaatcaag atatgtgttc atacaaatatctgaatataa atttttatag 130440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 130500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnngacagagcga gactccatct 130560 caaaaaacaa actaacaaac taaccaaaaa aacttgtttcttcacttttc ttcattccca 130620 gctaacacca agtgttttca ctttttatca tgatgaatataacagataaa acacatttca 130680 ctgtagtttc aatttcactt acattattgt cattgagatcttttcatata attaagttta 130740 tttgtatttt ctttctttct tttttttttt tttttgagacagagtctcgc tctgtcaccc 130800 aggctggagt gcagtggtgg catgatctcg gctcactgcaacctccacct cccgggttct 130860 aagggattct cctgactcag ccttctgagc agctgggattacaggcacgt gccaccacgc 130920 caggctaatt ttgtattttt agtagagaca gggtttcaccaggttggtca ggctggtctc 130980 gaactcttga cctcaggtaa tctgcccacc tcggcctcccaaagtgttgg gattacgagc 131040 gtgaaccatc gcaccccgcc ccactgtagc tttaaacaaagtcttgaaat aaggtagtgt 131100 acatcttcca gctttgcttt gatttttcaa gattgttttggttcttgttg gtctttggca 131160 tttccataaa atttttagag tcaatgtgtc aatttctaaaaaaaaaagtt ctatttgaat 131220 tttggttgga attgcgttga atctacagat cacttttggaagactattaa tcttaacaat 131280 attgtgtctt ctaatccatg attttgatat agctctccattcactgatgt tttatttaat 131340 acctctttca atgttttata tttttttagt gtagagatcttgcgtaattt ttatacattt 131400 acatttcagt gatttacatt ttttatgtga ttgtaaatggcattttaaat gttaattttt 131460 aattatttac cagttagcag aaatataatt gaattttgtgtcttgacctt gtacttttga 131520 ccttgtacta attcacttaa tacacctagt gttctttgggcattcgttct gatgtttctg 131580 tagaaacagt catgtcacct gtggataaag acaactttacttcttttcta ttctttaatt 131640 tcattgtgtt caaggaacat agtaaaattt caatattttggaaattttgc gagatgtgtt 131700 ttgtgtctta acacatgatt catgttgatg catgttccagatacactcta aagatgtttt 131760 tccatttttt tctggcatcc actgtttctc aggaaaaaattatccatcac ttatgtcact 131820 gtttttcttt atgtaatgtc attctccagc tactttcaatataatctctt tacttcagtt 131880 tttggtagtt gcactacgat gtgtccagtt atgattttcttgtacttatc ttgctggcat 131940 ttgttgagct tcttgatatg taagtagatt atttccaccaaatttggggg aatttcaatt 132000 atttttttct tcagctcttt ttttgttcca ttctctttcccttctcattc tgggactgaa 132060 attatactta tgttagacaa cttatactta tacttatgttagtcaggctt tgtgtccgca 132120 cccaaatctc attttgaatt ataatcccca taatccccacatgtcaaggg agagaccagg 132180 tagaggtaat tggatcatgg gggcggtttc ctccatgctgttcttctgat actgagttct 132240 cacaagtctg atggttttat aagtgtttgg cagttcatcctgcatcattc tctctcctgc 132300 caccttgtga agaaggtccc tgcctcccct tcaccttccaccatgattgg aagtttcctg 132360 aggcccccct agccatggag aactgtgagt caattacacctctttccttt atacattacc 132420 cagtttcggg aagttctgta gagcagtgtg aaaatagactaacacagatc atttaaaata 132480 tcccacacat cactgaggct ctgctcatta ttgttcatctttgctcccct tcattttcaa 132540 aatgcataat ttctaatggt ccttctccaa gttcatggactatagtggtc atgagaatga 132600 ccaagcctgt agctacggga agcataattg actgagggtccgagctgcca cgctctgaaa 132660 ttcatcacta tgtttgtgct gaggacgcac ttcccacaggctgcacccac tcaaacactg 132720 agtatggcaa acgtttaagg gcccctctaa tggctgactttggctcaagt actccccagt 132780 ggccttgctg aattttccct ggatttcaag acagtctaggaagcttccac ctaaccttcc 132840 ttccctctcc ccttcactcc aggacagact tggaggttcccacagccttc cccagttccc 132900 ttccccctca ttttctataa cgataatttc cctgaataaaagcctttcat gtttaatcca 132960 gtcttagcat ttgctctgaa gggacacctg aataaaataagttaaatcat gggcggattg 133020 acaaaacacg cagtgaggtg aggctctggg gttgactcacccactacctg gcaggcacac 133080 aggatgctat tctgttggca agtggagcaa agagagcccctggaaaaaga cagcagctct 133140 attgtggaat atttcactgg tggtgaactg agaaactgtatcagtggaga gcaatattgt 133200 aaaaagtaag ttaacagaga ttttgaggcc aggcacagtgggtcacgcct gtaatcccag 133260 cactttggga ggccaaggca ggtggatcgc ttgaggccatgagttcaaga ccaggctggc 133320 caatatggtg aaaccccatc tctactaaaa atacaaaaattagccaggca tggtggcgca 133380 cacctgcaat cccagctact caggaggctg aggcagaagaatcacttgaa cccgaaaagc 133440 ggagctttca gtgagctgag atagggccac ttcactccaggttgggcgtg agtgagactc 133500 catctcaaac aaacaaacaa aaaaacagag acttttgaaaaatatgggga aacaatacct 133560 acaaagtcag tggagttggc tggttattgc taaattgcattgacattctg cagaaggata 133620 atgagggatt gacgactatt agcaaaaatt tagagacaagccacagatct tcagtgagag 133680 cttacaaaga agcccttatt tctacagagg aatggtaggcccagctgagt gacaggctaa 133740 agacctcatt gttagagttg tagagctccg aagacatttgaatgctcaac caaggcagga 133800 ttgttatgtg aaggacagag gactggtgga caaatctgtgatccagtcag atgggatgaa 133860 gaggagggat aggatgagtt ccaactgtgg cccccgagagcaactatggt aacaggggct 133920 attgtctatc acactaactt ctctcttctg agtttcccctcaggaagaaa ggctggcagg 133980 agctataaag gagttgctcc tcgcacttat gtagaaacatgactttgtgg agcagaagtg 134040 gtcaatggca gcagccacaa gaatatacct ctcagatctccagtttcagg gactataatt 134100 gctcacaggc catagctact gtgctctgaa atctattgctgcatttgcat ctggagtatg 134160 cttgacatga atgacggtgg cagggagcta agggaagcctgctcctggga gacactggag 134220 ccctctgctg gctggctttg actcaaggac tctccgttgtctttccaaac ctctcttcac 134280 ttggggtcag acttgcgtta caatctgatg gttgtcccaatcttctccag ctctgtctcc 134340 attttctctc gcaagtgttt ccactaataa aatccttgcagttttgatat gaaaaaatgc 134400 tcaacatcac taattatcag ggagaggcaa attaaaaccacaatgagaca ccaccttact 134460 cctgcaagaa cggccataat taaaaataaa aaaaaaaatgttggtatgga tgtggtgaaa 134520 agggaacact tttacactgc tggtgggaat ataaactagcacaaccatca tggaaaacag 134580 tatggagatt ccttaaagaa ctaaaagtag atctaccatttgatccaggc cgggcgcggt 134640 agctcacacc tgtagtccca gcactttggg aggccaaggcaggtagatca cttgagatca 134700 ggagttcgag tccagcctgg ccaacatggt gaaaccccgtctttactaaa aatacaaaaa 134760 ttagccaggt gtggttgtgg gcacttgtag tttctacaagttgtagctac ttgggaggct 134820 gaggtaggac aattgctgga acccagaggg cagaggttgcagtgagccta gatcatgcca 134880 ctgcacttca gcctggatga cagagtgaga ctctgttgaaaaaaaaaaaa aagaattacc 134940 atttgatcca gcaatcccac tactgggtac ctatccagaggaaaagaagt cattatatga 135000 aaaagacact tgcagatgca tgtttatagc agcacaattcgcaattgcaa aaatatggaa 135060 ccagcctaag tgtccatcaa ccaacaagtg gataaaatgtggtatatata cactgtgaag 135120 tactactcag ccgtaaaaag gaataaaata atgacatttnnnnnnnnnnn nnnnnnnnnn 135180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 135240 nnnnnnnnnn nnnnnnnnnc ccgtatttcc attttacctatgacagtctc ccttggcctc 135300 actactgagt tatcagtgat aaaaatgcca tggttacataatttatatga cttacctcag 135360 atgttctgta atatagtact ttccccttac ctcaggggatacattccgag accccccagt 135420 ggatgcctaa aacaatggat agtaccaaac cctatatctactgtgctttt tcgatccggt 135480 aaccaagaca ctactaagtg actaacaggt gagtagcagagatagggaca cctctgtttt 135540 attgtgcttc acttcaccgt acttcagata ctgcattttacagactgaag gtttgtggta 135600 accctaaatc aagcaagtct atgcgtgcca tttttccagcagtgcttact tcatgtctct 135660 gtgtcacatt tttgtaattc ttaagatatt tnaaacgttccctttttttt tttttttttt 135720 tggagatgga gtcttgctct tgtcacccag gctggagtgcagtggcatga tctcggctca 135780 ctgcaacctc cgcctcccgg gttcaagcga ttctcctgcctcagcctcct gagtagctgg 135840 cgttacaggc acacaccacc acacccagct aatttttgtatttttagtag agacggggtt 135900 ttgggttttc ccgtgttggc caggctggtc ttgaactcctggcctcggga tccacccacc 135960 tcggcctccc aaagtgctgg gattacaggc ataagctaccgcgcctggcc caaacttttc 136020 cattattatt atatctgtta ggtgatctgt gatcactgatctttgatgta actattgtaa 136080 ttgttttggg gtgtcatgaa ccatgcccat ctacatacgacggcgaactt aatacataaa 136140 tgttgtgtgt gttctgactg ctccaccaag cagccattcccacttctctc tcccttacct 136200 cagacctccc ttttctgtga gacacaataa gattgaaattaggtcaatta ataaccctac 136260 aatggcctct aagtgttcaa gtgaaaggaa gagtcaccacatgtctcttt ctttgtttgt 136320 tttttttttt tttttttttt tttgagacag agtctagctctgtcgcccag gctggagtgc 136380 agtggcacaa ttttggttca ctgcaaactc cgtttcacaggttcaagtga ttctcctgcc 136440 tcagcctccc aaatagctgg aattacaggc gcccaccaccacacccagct aatttttgta 136500 tttttagtag agacggggtt tcactgtgtt ggccaggctggtctcgaact cctgacctca 136560 tgatcctccc acctcagcct cccaaagtgc tcggattacaagcgtgagcc actgggccca 136620 gccgtctctc actttcaatc aaaagctaga aatgattaaacttactgagg aaggcatatc 136680 aaaagctgag ggaggccaaa agctacactt cttgcaccaaacaacgaagt tgtgaataca 136740 aagataaagt tctttttttt tttttttttt ttttttttttgagacaggag tctcactcta 136800 ttgcccaggc tggagtgtag tgtgactcaa tctcggctcactgcaacctc tgcctcctgg 136860 gttcaagcaa ttctcctgcc tcaacctcct gagcagctgggattacaggc gcccaccacc 136920 atgcctggct aatttttgta ttttaagtag agacggggtttcactatgtt ggctaggctg 136980 atcttgaact cctgacctta agtgatccgc ctgcctcagcctcccaaaat gctgggatta 137040 caggcgtgag ccatcgtgcc tggccagaaa aagttcttgaaggaaattaa aagtgctact 137100 ccagtgaaca cacaaataag acagtgaaac agccttattgctgaaatgga gaatgtctaa 137160 gtggcctgga tagatcaaac caaccacaac attctcataagccaaagcct aaaccagagc 137220 aagaccctaa ctctaactct cttcaattca atgaaggctgagagtgctgg aagcaatgga 137280 agaaaagtct gaagctagca gaggttggtt catgaagtttaaggaaagaa gccacctcca 137340 taacaaaaaa agtgcaaggt gaaccagcga gtgctgatgtaggagctgca gcaagttatc 137400 ccgaagatct agctaagatc atgggtgaag gtggatacactgaacaacag attttctttt 137460 ttgtttaatt tttatttttt gtagagacag aatctcgttttgttgtccag gctggtcttg 137520 aactcctggg cttaagtgag cctcccacct cagcctcccaaagtctggga ttacaggcat 137580 gaaccaccac accagaccac agatttttca atgtagacaaaacagcctta tattggaaga 137640 agaggccatc taggaatttc gtagctaaag aaaaattaatgtctggcttc aaaggacagg 137700 ctgactctct tcttacgggc taatgcagct gatgactttaagttgaagcc attgctcatt 137760 taccattcca aaaactctag ggctcttaaa aatgatgctaaatccagcct cgcctacatg 137820 gcgaaaccct gtctttacta aaaatacaaa aattagctgggtgtgacggc atgcacctgt 137880 agtcgcagat actctgtagg ctaaggcaga agaatcacttgaacctggaa ggtggaggtt 137940 acagtgagcc aagatcgcac cacttcactc aagcctgggtgacagagtgc aactctgtct 138000 caaaaaaaaa aaaaaaaaaa agctaaatct actctgcctgtgctctgtaa atggaacaac 138060 aaagcctgca tgacagcaca tctgtttaca gcatggtttactgaataatt taaagcccac 138120 tgttgagacc taccgctcag aaaaaaaaac attcctttcaaaatattact actcgagccg 138180 ggcgcagtgg ctcatgcctg taatcccagc aatttgcgaggctgaggcag gtggatcacc 138240 ttaggttagg agttccagac cagcctaacc aatatggtgaaaccccgtct ctactaaaaa 138300 tagaaaatta gctgggcatg gaggcgcatg cctgtaatcccagttactca ggaggctaag 138360 gcaggagaat tgcttgaacc cgggaggcgg aggttgcagtgagctgagat tgcgccactg 138420 tattccagcc tgggcaacaa gagcaaaact ccaactcaaaaaaaaaaaaa gttatatata 138480 tataactgct cgttgacaat gcatcagatc atccaagagctctgatggag atgtatgagt 138540 agattaaaag ttgttttcct gtaatcccag cactttaggaggccgcagca ggttgatcac 138600 ctgaagttgg gagtttgaga ccagcctgac caacgtggagaaaccccgtc tctactaaaa 138660 atacaaaaga atttgctggg cgtggtggcg gttgcctgtaatcccagcta ctgggagact 138720 gaggaaagag aattgcttga acccgggagg cagaggttgcggtgagccga gatttcacca 138780 ttgcgctcca gcctggacaa taagagcgaa actctgtcccaaaaaaaaaa aaaaaatagt 138840 tgttttcatg cctgctaacn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 138900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnna 138960 aacactgcag ggtttacttg acttgcccaa gatcactaaactagtcctgc atgttttctg 139020 gtttaccata ttgtttcaca gtgccttctt atacttgtaattgcagctaa tatagagaaa 139080 acatatttca agaatttttt tttttttttt tttttgagatggagtcttgc tctgtcgccc 139140 aggctggaat gcaatggcac gatctcggtt caccgcaacctccacctacc gggttcaagt 139200 gattctaatc ccagcctcct tagtagctgg gattacaggcatgtgccacc atgcctggct 139260 aatttttgta tttttagtag agttgaggtt tcaccgtgttggccaggctg gcctcgaact 139320 cctgacctca ggtgatccac ccgccttggc ctcccagagtgctggaatta taggtgtgag 139380 ccaccgtgct cacttatttc aaggattttt tctcattttcaaaaaccgaa tataccacat 139440 ttagagaaat taaatgtaat gtgtttagat tttaaatagaaatagtagca tgtgagaatg 139500 atattaacaa atcagaaaaa gaaacattac ttgtaattctaccgtcctta caaagtgaac 139560 tgttattgca gttctgcttc aagtatttat ctctatgcattaatgatttt acatactgct 139620 aatatgattt ccctaatatt tcccaaatct tttggtaactttgacttcca ggcttttttt 139680 tttttttttt ttccccttaa agttaggatc tttctgtgtcacctaggctg gagtgcagtg 139740 gcacaatcat agctcactgc agcctcgaac tcctgggctcaaaggatcct cctgcctcag 139800 ccttcagagt agctgggact acaggtgtgt accactatgcctggttaatt tcaaaaactt 139860 tctgtggaga tagggtgtca ctttgctgtc caggctttcaggcatatttt tttttttttt 139920 tttttttttt tggctagaga gtctcgctct gtctcccaggctggagtgca gtggtacgat 139980 cttgactcat tgcagcttcc acctcccggg ttcaagcgattctcctgtct cagcctcccg 140040 aatagctggg attacaggca cacttcatca cacccagctaatttttgtat ttttagtaca 140100 gacagggttt caccatgttg gccaggctgg tctcgaactcctttcctcag gtgatcagcc 140160 cacctgggcc tcccaaaatg ctggattaca cacatgagccaccatgccca gccgcttttg 140220 ggctttttaa gctggtctgc atgactctac tttctgtttttcacgcgtat catttttaat 140280 ccttgaacat tgttttgtat cattctacaa atgatcaaatgtcttttata ggtaccagtt 140340 gcctccctta gcgtttccca aagggtggga aacgcttaaacattaacacc ccagtcttac 140400 actgtcagct gcatgtaaaa aaagcacaaa aaactaaaggtattaaacag taatgagttg 140460 gttatatcac caacatttct cagggtggca ggaagggcactgcaattctt gggttaattt 140520 gggtgcttga caatgtcctc aagaaccaag attcttttggtcattctccg ccatcctcag 140580 cttgttggtg tttgtcttcc agcttgtctt ttcactatcccaagatattt gcagcacaca 140640 cgactcatac cctcacacag ctttgactgg agtcagaaaaaggctcttta tgttcaggct 140700 aaatgctttt aaaagaattt acctacacac attgatgtcccaaatcttag tgagacttca 140760 ctgtatggtc atgcgtcagc cagtcactga caaggaaaatgggattacca taactgatgt 140820 agaatattca agatttactc cagagcctgg gaaagggtctattttttgag gtaagcatgt 140880 acaaactgtg gggttaatat tgaaaaagat caggaaatggcttgattagg caaccagcag 140940 tgtgtgctat aaaattgaat ttacttagtt cccttcaaggctcttcataa tctgacccca 141000 tgataattta atataaaacc tctattctag acaagccaatcagttgtctt tcctgcctat 141060 ctatcctgct catgtgcatt cctacctgta ccttgatatacattatttca ctttcttaga 141120 aagttctccc tgtttattag agctaatgtg gctgttttaattaaaattta aaatttaatt 141180 ccttaggcac acaagtccca cttcagtagc cctgtgactaagcggctact gtattggata 141240 atgaaggaac ggaacatttc cataatcaca ggaagttctgacggaaagca ctgctctaga 141300 aattcttaca aagcatacct attatttaca cagtgtttgtcaaactatgg accacctaga 141360 tgagaatcag ctgtttgaga tgctgtttaa atgcagattctgagccctag gaataggccc 141420 tgagccactt tcatgcccaa taaagatgaa gaacactgctttgagatcta gttttgtttc 141480 ttttatggaa atatactgac cactggagtt ttcattaattttctccttct ctagacttct 141540 atagcattca tagtttatat ataaagtttt agaatttctttacaatatta atattctcta 141600 attgtctcag aaagtattct tcctgaatat tagagtataagctttttaag aggaggtact 141660 atggcatatt atcacttgta ttttccaaaa catcgaatataaaatagggt gcatagtaaa 141720 tatgaagtat tgactgctta attacattct acagaattgtcaatctatag tatattcact 141780 tggcagttct ttgaaaatca taatactgtc tgcaatattgaatatggatg atagagtatt 141840 tctgtaattt ttttcnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 141900 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnaaatg 141960 aataaatgca gtctctaaag cgagagtttt tcaaattagtaagatataat ccatcagtga 142020 gtcagttgtg aattcaattt agtgggttgt gactggcattttaaaaaagt gaaattggct 142080 ggccattgtg gggctcatgc ctgtaatccc agcactttggaaggccaaga taggaggatt 142140 gtttcagcct aggagtgtga aatcagccta ggcaatatgtgagaccccag tctctacaaa 142200 aagtagaaaa aatttagcca ggtatggtgg cacacacctgtggtcccagc taaatgggag 142260 gctgaggtgg gaggattgct tgagcccggg aggtggaggttgcagtgagc tgagatcgta 142320 ccactgcact ccagcctggg cgacagagag cgattctgcctcaaaataaa tgcataaagt 142380 gaaaatagac taatataaga aatcattgca cacagtaagaggaatatggt taatgaaaca 142440 tttgtttcag tttcatatat atgtatatat gtactataataatgtgtcac aaaaaagaaa 142500 ccgtcattcg atctgttttt aatatacgga atttaatacaggaaactggt tacacaaatg 142560 ataacgctga gaagccaaat aggggaaggt gaggcaaccagagattagca gcagcagaaa 142620 ccactaatcc ttttcgacta gagggacaaa gagaggaggcagtgtggtta gatcccagaa 142680 ctggactcat ctaatgacag gagggccttt ctggctggagctggagccag ggaggagatt 142740 tagccactgt cagagatgca cctcgggcag agaaaggcaaggagaatcat cctagctttt 142800 ccattcttcc cactctccag cgttgtggca gggcctcgtatcagtaggac ccagccagta 142860 atcacttaac aacatgagag cctcagaaac acagcctgttgggatcagca tacccatgac 142920 attgcagcag aataggaaag gatttgagag caaacaggccaggggccgcc acagttgtga 142980 agtaatatat atttctgatt gaggtttggg ttctaaaagatttaaaagcc attaccttag 143040 acgacaaagc taccctctca attttatgtg gttctctctacatattgaaa gaataaaatc 143100 atacttttga tcattagggg atagcaaatt ttgccctttttaatgttctg gtggaaggca 143160 gtttgaactg aagaagtcag atggggagaa taatccacttctctcagtag tgttttgttt 143220 tgttttgttt tgttttgttt tagagacagg gtcttactctgttgcccagg ctggagtgca 143280 gtagcaggat cacggctcac tgcaacctct tcttgggttcaagtgatcct cccacttcag 143340 cctgtcaagt agctgggact agaggcacgc accaccatgctcagctaatt tttaagtatt 143400 ttttgtagag atggggtctt gctatgttgc cctcccaaagtgctggaatt ataggtgtga 143460 gccaccatgc ccagaccctt tttagtttag gagtagtcttataaagctct cttcgtaaag 143520 aagttaaaac ttctaacaga tgcaggtgaa atataggctagaaacaacag aacaggaaag 143580 aagtacacaa gcagcaaata tgtaagcaaa ggaaccagatgcttctctcc ttgactgtgt 143640 acaaggaaac tcatagtacg tgcttaagaa ttggcaagctgagtaccaaa ttgcctctgc 143700 tggcagctat atgtgtgaga attctacaaa attgcctggatccacaagga catcagcgac 143760 caaaattcat ttacagcacc gtatttctga ctggtggctatctgttactc tggttccaaa 143820 actctgacct tcaattactt tagatttctg atcccatcccaagaaatggc tcccaaagct 143880 ccctcagccc tttactacct ccttgatttg agaccaaacctaatgcttga tcccttgtgc 143940 ttggaaacat cctagcaccc acttttggac ttctaccctgtaccgacggt gcacaaacaa 144000 tgtacttcaa tctgttcatt ttctctctgg tctctttcagtcaactttaa tacaattctg 144060 gcaatatcat tttaaactca actgtataat tatcaagagttatttttaaa ggcaatgaat 144120 atttgttggg tgaatgagtg gatggatgga tggatggatggatggatgga tggatggatg 144180 gatggatgga tggttgtttg gttgnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 144240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 144300 nnnnaacttt gcccagttcc tccaccccac actattcttcacattaactg attacttaat 144360 aattaaaata agaaaatatc agcatgaaac atgtataagaatggaaaata agtaaataaa 144420 ttaaaaatgt gaaagaaaca agcaaaaagt taaaaagaaaaaccattacg cctgtaatcc 144480 cagcacttta ggaggccgag gcaggtggat cacctgaggtcagaagttcg agaccggcct 144540 ggccaacatg gtgaaacccc atctctacta aaaacacaaaaaattagccg ggcatggtgg 144600 caggtaccta taatcccagc aacctgggag gctgaggcaggacaatcgct tgaacccggg 144660 atggggaggt tgcagtgagc cgagatcgcg ccattatactccagcctggg caacaagagc 144720 aaaactccca tctcaaaaaa acaaaaaaca aaaaacaaaaaaaaaaccta agaaaaacaa 144780 tccaaggaaa catgaaaaat tccaacaact cattctgtgtagtctcaaaa aaatcagata 144840 ttattatctc tctactaaaa tgtatctgtg aagagaggtaagcatttaaa gaggacatta 144900 tgaaataacc taggctaggt gtgatggcgc atgcctgcaatcccaactct tggggaggtt 144960 gagtggacag attgcttgag gtcaggagtt tgagaccagcctgcccaaca tggcaaaatc 145020 ccgtctctac taaaaataca aaattagctg ggtgtggtggtgtgcacctg taatcccagc 145080 tacttaggag ggtgaggtgg gaggatctct tgagcctgggaggcagaggt tgcagtgagc 145140 tgagattgca ccactgcact ccagcctagg tgacagtgagaccccccatc tcaataaaat 145200 aaaataaaac aaaataaaat aacctagaga gttttaaaagcaagctggca aggcttgctt 145260 ttaaagcaag gctgatccat gtgtcagagg tccttcctttttaaggcaga ataatatccc 145320 attgtatgta tttatcacat tttgtttatt gattcatttattgattcatc tgtaatggat 145380 acttgggttg cttctacctt ttgactattg ttaataatgctgctatgaac atgggtgtac 145440 aaatctctct tgagtctctg ctttcagttc ttttaagtgtatatccagaa ttactggatc 145500 atgtgtggca attctgtttt taattttttg aagaactgccatactgtttt ccacagcagc 145560 cgtaccattt tacattcctg ccagcagcgc acaaggattccgatttttcc gcattcttga 145620 caatacttgt tattttattt tttctttctt tctaatagtagccatgctgc tgggtataag 145680 gtggtacctc attgtggctt tgatttgcat ttccctagtgattagtgttg ttgagcatct 145740 ttcatgtgct tgttggccat ttgtatgtct tctttaggaaaatgttactc aaattctttg 145800 cctgtttttt gatctggttg gtcttttggt tttttgtttgtttgtttttt gagatggagt 145860 ctcactttgt cacccaaact ggagtgcagt ggggcgatcttggttcactg caacctctgc 145920 ctcctgagtt caagtgattc tcctgcctca gcctcctgactagctgggat tacaggcaca 145980 cgtgctacca cgcccagcta atttttatat ttttagcagagacagggttt caccatgttg 146040 gccagcttgg tcctgaactc ctgacctcaa gtgatctgcctgtctcggcc tcccaaagtg 146100 ctgggattac aggtgtgagc cactgcgccc agcctggtcttttgttgttg ttattgagtt 146160 taggagttcc ttatatagtc tgagtattaa ccgcttatcagaaacaagat tcgcaaatgt 146220 tttctgtcat tctgtaattt ttcttttccc tctttgattgtgtcctttga tgcacagttt 146280 tttattttga tgtagtctgt tttatctgtt attacttttgctgcctgcgc tttcggtgtc 146340 atattcagaa atcattccca aatccaatgt catgaacccctgggttttag taagtatgtt 146400 ttaactgttc ataacttcta actaatctgt aattgcagattatatttatt ttttatatag 146460 aaaaaattaa ctcannnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 146520 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnatggca 146580 gaatccttct gggtagttac tgtgacgatc cttcaccttggcagtggagg aagttctatg 146640 attacatcct ggttctgctc tcaaggatga acaccactttgcatttattt ctgtggccct 146700 tggctccaca ctctggaata ttcttccttg ttcattattctccatgacca tatctgaagt 146760 gcaactagga agtaagccct ccagagactt cacagttttcacagcgcatt tcctgctggt 146820 ataagtttgg agtcctgagg gctcattcat acttaaggattttgttttgt ttttggtttt 146880 gtttgtttgt ttttcctttt tgtggacaac gaggtcttgctatgttgccc aggcaggtct 146940 caaacttctg ggctcaagcc atcctccagt ctctgcctccctaagtgctg ggattatagg 147000 cataagccac gactcctggc ctacatgtaa ggcattttaaaggccaggtt tatagctgat 147060 ttgaccttac ggttttctta gacacttagt agactattctcttcctatga gttccatgga 147120 ccagtaatca tacccaaagt tctttcctgg gcaaagctgcaaatacttta tttctttgct 147180 tcctcactgt gtttcccttt ctctcaattt aagatctgaggcttagtggc tcagcatacg 147240 catgcattat aagaattcac cttctggccg ggcgcggtagctcacgcctg taatcccagc 147300 actttgggag gccgaggcgg gccgatcaca acgtcaggagattgagacca tcccggctaa 147360 catggtgaaa ccccgtctct actaaaaata taaaaaattagccaggcgtg gtggtgggcg 147420 cctgtagtcc cagctgctca aggaggctga ggcaggagaatggcgtgaac tcgggggcag 147480 agcttgcagt gagccgggat catgccactg cagtccagcctgggtgacag agtgagactc 147540 cgtctcaaaa aaaaaaaaaa agattcacct tctactagaaagggtgctac tactttccca 147600 ctcaaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 147660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnntga catctacttt 147720 acagcagata caaaaattaa cttaaaatag atcacagatctaaatgtaag agctgaaact 147780 gtaaaactat agagttattt gacactggat taggtttcttaatatgacac caaaagtacg 147840 agcaacaaag gaaaaagcta gataaattga ttcataaaaattgaaaacct ttgtatatcg 147900 aaggacagaa tcaagaaagt gaaaagacaa cccataatacgggagaaaat atttgcaaat 147960 catctatctg ataaatatct attatggaga tatataaatatctattatgt agatatataa 148020 agaacatata tgtatatata taaagaatat ttgatgcttcttgcaattaa ataaaactca 148080 ataaagaggt aaatagagaa aacaaaaagt actggagagtaaaagccccg tgggatctaa 148140 aaaattattg ataatgtttt tataaaatct aatacaaatgctatatagca tagaaaaaac 148200 aaaaattgaa atattaaaaa taatctgtaa ttaaaatcccagtttatttc aataaatttg 148260 ggtggtatgg gcccttgggg agtgaatttt aaccgttaaaagtctcacta tgtttggaga 148320 agagaatggg aaataagaaa atatatacca ccaggcacagtggttcacgc ctgtaatccc 148380 agcattttgg gaggccaaag caggaggatc acttgagcccaggagtttga gaccagcctg 148440 ggcaacatcg tgagaccttg tctctataaa aaataaacaaattagtcggg tgtggtggca 148500 tgatcctgtt gtcccagcta cttgggaggc tgaggtgggagaatcactta aggccaggaa 148560 gttaaggcta caatatccat tatggtgcca ctgcactccagtctgggtga cagagatcct 148620 gtcttaaaaa gagaaaagat acatcctttt tttctttataagcatatcag ttatttataa 148680 acatatcagg tttttttttt gttttgtttt aaagatactgaccacaactt cttgtagaac 148740 tttcaaataa tttgagaaga aagaaataca aagaaaccaaaaccaatacc acttcaccaa 148800 caccacaaaa gtcaggaaga aaagggaaaa acaaagcataaaaaagagaa aacacaattt 148860 aaggtgtatg aatacattct tagcatcaat aacaacaaattcagtgggct aaatttccct 148920 attgaaagac aaaaatctct ctgaaaaaac agaagaaaatacagctatat tctctttatg 148980 aataatatct gaattaaaac aacatagatt gaaattaaatgaatggccaa caaaggtaaa 149040 acaagtgaag acaaaaagaa gcaaaagtag caatataaaatcaaagtgaa attgatgatt 149100 taaaagaaag catgggccgg gctgtgtggc tcatgcctgtacatcccaag catttgannn 149160 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 149220 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggttccttttcgg gatttaaccc 149280 taaaggaggt tttacccctg actcatattt ggtaattcagatttaaccag aaaactaaag 149340 tttgtagtag ggttgaaaaa ttttcttttg cttcggctttcccctgaacc cggttgtggc 149400 aaaacccaat gggcctttac ctccttttcc ccctgctccccctgccaaaa aaggggtttt 149460 caggaaatgt gaagtgtttt tcctttaaag atccctgtaaaatgttaaac ttttaattga 149520 ttaagccccc ccttgggggg aaatgtgaaa aaactttttttatggaactc ccttttttta 149580 aaagggaact gctccaactg gtctcctacc cttgttcccctattaggagc aaagaattta 149640 attcctggaa ttttttgccc ttgattggcc cctctgttacaaaatttttc cccccccttt 149700 ggtttttttt cccccccccc cttttttttt tttttttggagacggagtct ctttcaccca 149760 ggctggaggc atgattttgg ctcaccgcaa cctctgcctcccgggttcaa gcgattgtcc 149820 tgtctcaacc tcccgagtag ctgggattac aggtacccaccaccaccatg cctgactaat 149880 ttttatattt ttagtagaga tggggtttca ccattttggccaggctggtc tctaactcct 149940 gatctcaggc agtacgcctg cctcggcctc ccaaagtgctgagattacag gagtgaacca 150000 ccgtgcctgg cctcctctac atttttaaat tgatgattaagctatatgat ggacatgttg 150060 tgaaagtaga aggtagtttt gctcatgcct aaatcataaaattaagtgaa ggatcagtgg 150120 aagtatatcc tttgattatc attcctcgtg aattgtttttgcacttggat gaatgttatg 150180 ttaacattcc gagtcctggt actagaattt ggacttttaggaagtgtgga agtcttatct 150240 gcattatttt atggaacttc agagtattga ataaaagctaaggttcctgc ctcatttata 150300 gttggacata cacattcctt gcattgtgca gcttttccactgcattaaaa tggaagcctg 150360 catccctctc tcccacccca gtgtcttgac tccccttagaaacctcagtt ggtatatgct 150420 ttggttaagt tagaagaata cattcttgcc aactttatttccctaataaa ggcacacaag 150480 ttttgcttat gggtgatgct gaaaagtaat agactatcccatggtattgt agaattaaag 150540 tgtgtggtac caggtccaaa cacttagtga ctatatgtaattagtggagg cagctcattt 150600 ttcattaaca aacacatgat caaatacttg gtcacgcaaaactcagatgc ccatgtgtaa 150660 caacaacaaa agtactttgg attactgcat tggggaagggtttaacatca tgacctaaga 150720 tttactttta aggttttatg accacgtgac taaaatgtctgttttctgtg taggtcattc 150780 ctgttaatag ggagactaac attcagagct ttcaaatgtcagtaacagaa ataaaagaga 150840 gagaagtgag actattaagg tccagacact tcacatgttaactcatttaa ttctcacaaa 150900 tctgttatgt gttgtaatca ttatccctca ttttacaaaaaagaaaactg aggctcagaa 150960 aggttatgtg acttgttctc ccaacggtat catgtaaatatactattttt ttctttaaaa 151020 tgcttttgcc tggttcttta tttttttaat gttttgacctgtctggaact gattacatta 151080 ttaagcatca cgcaaaaacc attcttttcc atagtgctattcaattatac aaacaacatt 151140 tactaaagag tgagacttta tagtgaagat tgcttaggattatctgaagt tcttccagtt 151200 cctgccatgc agcaggatat agtctgaata gatgctttcccacttcaaca ctgcttggta 151260 taccttcaca ctgccatgca cttgcttaga taatatttaaatatccactt aaaagcaaaa 151320 gttcggccag gcgtggtggc tcacacctgt aatcccagcactttgggact ctgagacagg 151380 agcaattgct tgaggtctgg agttcaagac cagcctgggcaatatggtga aactctgtct 151440 ataaaaagac gaaatttagc caggtgtggt agcgtgagcctgtagtcccc gctactcaag 151500 aggctgaggc aggaagatgg cttgagccca ggaggtggaggttgcattga gccaacattg 151560 caccactgca ctccagcctg gccaacagag caagaccctcaaaaaaaaaa aaaaaaaaag 151620 cagaaaaaat gcaaaagctc aggtgtgtcc tttactgctagctttcccta gaaagtgcct 151680 caaagaaaga aagaagaccc atgacaatct aatacatttttataattaaa aatgtatata 151740 gcttgcattt gtgattatgc agtatttatt ttatattaaacctcacattt tattattatt 151800 tagctgttac cttataaaat atttatagta acaaatgtaggtttttgatg tctaagataa 151860 agataaatgc agtagtaagg agaagtaatg caaaataaaatttcatgtcc ttatgagggg 151920 aaaagtgcat ggattgaaat cacttgcagc caatctgtggtaattttctt gcccctgggc 151980 ctggcagact tagaaggtaa aaagaactgc atatgaaatgaaaaccactt ttctcccagt 152040 actcatctaa ccttgtagag ggtgttaaat gtacacaagatgtgttaggg gaaattaaat 152100 attaaagcgt atattttatt ctttcataaa aaaggagactgtataatgtg atccaaaaaa 152160 ctaaccattg tttgtttgtt tctttaggat ggattggatgctttggtata tgatttggat 152220 tttcctgcct taagaaaaaa caaaaatatt gacaactttttaagcagatg taagtaattt 152280 tttgtggagg ttaatgacag acttcatata actacattttcacttatgtc attcaagccc 152340 ttattttttc ttctatttac tttttggtga tttcatcaatgatttctaca tagaatttag 152400 taggaagtgt gaaagaaaca atttggatgc ctgttaggagaaccaaaata ttttgaagag 152460 aaagggatga aaaatagtta aaataagtat tcaaaaaactgtgcattgag tttgaataac 152520 tgcacattga gtttgagtaa ctgctgagac atgagaatttttctccatga aagctgtttt 152580 atatttaaat tttacagata ttcttttccg aaggctcctttttagattgg attttatcat 152640 ttaaaaattt gattaaataa cttataattt caaacttttaatcttaaaga attgataaga 152700 tttagatcat aaagtcctcc ttactttatt tatttctgtttctgaactat aaatattgat 152760 acatgtaagt ttgattggtt ttggtgttat ctttcaattttggttatata tgttttatta 152820 caaaggtttt gtttttctaa atagtcatat ctttttgattttttaaatca cttctatact 152880 ttgaaatctt gtattagaat tttttaattt tatgatcttttagtttttca atttttaaaa 152940 aattattgtt ttattttcgt ttttgaatat ggtgccagattctgaactga gaagttttta 153000 agtataaaag ttttaattat ttctcatgtc ctatggacatagcagtaaaa ttttattaat 153060 atttacagtt gcttacctcc ctacctgaca acatagtgaaggaactaaaa agatggagag 153120 gcttgactat ttatcttaat ggtaactggc atttatagagatggtgtaga taaggggtaa 153180 atgctcctta gtacaaaggc agagggtggg gcatggttttttttaaagta atggctagca 153240 agaatgaaat tcatgcttcc ttaaactgtg ctactttgttgctttcataa tgttaagtat 153300 gcttgtatat tttataaata gagacattaa tgctttagtagaaataagtg aaataaaagt 153360 gtaaatctaa agtaaactgt tttaactggt aaacctgtacaatttatttt aatttttaaa 153420 ttggagaaaa atgcagagaa taatataaca aaattttagggcagtcatat gtagcagtag 153480 gtaaaaatca tgaccaagga tagtttatgc caggattactaagttagtat aaaattcaaa 153540 aatcagttag cttaatttac cacattaaca tattttaaaaaggaaattta gtagatgcag 153600 aaaaagcatt tgataaaatt taatatccat tcacaattttaaaatatctg tctgcaaact 153660 aggaagtgaa ggaagcttcc tttccccaat aaagggaatctacaagaaac ctacagttta 153720 gattgtatct acttgtttaa gacgaatgct tttcccctaagattggaaaa ggcaaatctg 153780 tcctctctta ccacttttat ttaacattgt cttagaagttctagccagtg ccatcaggta 153840 aaataaagaa aagccgtaca gattgaaaag gaagaagtaaaactttccca atttagacag 153900 catgattttc catatagaaa atcctcaaga ttctgcaaaaaagctacatg aactaataag 153960 tacattcagc aaggttgaaa gttagaagat taatatccaaaaagcagttg cacatacatt 154020 tattaacaac aagcaattgg gaattaattt attataacaaatacagcacc atttacaata 154080 gaagcaaaaa catgaaaaat ttaggagtaa atttgacaaaatatgtgtaa acctgtttac 154140 tgaaaactat acagcattgt agacagaaat taaggaagacctaacaagat attcaatgtt 154200 catggattgt acaattcaat attaaaatgt taacagtccccaaattaatt gatctataga 154260 gtccgtatat tctcagaacc ttagcagaca gttttttgtataaggtgacc agcaattctg 154320 taattcatat ggaaatgtaa aggacctgga acagccaaaacagtttcgaa aagtaagagc 154380 aagttggagg attaaggtca atatcaagac agtatagtcttgatgaaaga ataaacacat 154440 agttcagtgg aacagaaaag agcatctaga aatagacctgtgcatgcaag tatatggtta 154500 attgatttca acaaaggtga caggataatt caatgttcaagcctcagttt ccactgtata 154560 caaaaattaa ctcaaaatag atcatcaacg taaatgtaagagctaaaaca ataacacttc 154620 tagaagaaaa catagaacat atttgccact ttccttaggcaagtttttct tagataggac 154680 acaaataaca caaaccattt ttgaaaaagg ataaattagacttatcgaaa tttaaaatag 154740 ttctcttcag aagacactgt tcagaaaatg aaaagataagccaaaggctg ggaaaaaata 154800 gttgcaaagc atgtaaaaga agaaagaata gtcctttcaaccagtggtgt tcagacaact 154860 ggatatccgt acgcaaggga atgaatttgg actcctacctcatatcatgt acaaaaatta 154920 accgaaaata taaaagttaa aaatgtaaaa ctcttaggagaaaacatagg ttgaaatctt 154980 catgacctcg gactaagcag tggtttctta gacgtaataccaaaagcaca agcaacaaaa 155040 gaaaaataga taaactggac atcatcaaaa taaaaaacttttatttatgc ttcaaagata 155100 ctatcaagag tgaaggccag gcatggtggc tcacgcctgtaatcccagca ctttgggagg 155160 ctgaggcggg cagatcagtt ctgtccagga gttccagaccagcctggcca acatggcgaa 155220 accccatctc tactaaaaat gcaaaaatta gcagacatgttggcagacac ctgtaatccc 155280 agctacttca gaggctgagg caaggagaat tgcttgaacccaggaggtgg aggttgcagt 155340 gagccaagat tgcaccactg cactccagcc tgggcgacagagcaagactt tgtctcaaaa 155400 aaaaaaaaaa aaaaaaagag tgaaaagaca acttacaaaataggaaaaca ttagcaaatc 155460 atatgtccaa taaaggactg gtatctagaa tatataaacaagtgtaacaa ctcaataata 155520 ataagacaaa tagttcagtt taaaagtagg aaaaatatttgaatagacat ttctccaaag 155580 aagatgtaaa aatggccagt aaacatgtgc agaactgttcatggtggtta gtcattagga 155640 agttgcaaat tatggccaca ataagatact agtacacctctattagaatg attaaaactt 155700 tctaaactga caatatcaag tatttatgaa aatgcattgcagctggaacc ctgtacattg 155760 ctgactggta aatggtaagg cactttggaa aacagtttggttgtttctta taaagttata 155820 catgcacttt actatatgac cccaggaatt ctgctcctagatatttactg aaaagaaatg 155880 aaagcatatg tctgcacaaa gagctgtatt aaaatgtttaaggcaacttt atgtttgctg 155940 aaaactggaa gcaactcaag tgcccgtcag ctgatcaatggataaattgt ggtacaacca 156000 catagtggga tattactgaa taataaaaag taatgaattactgctatgtg caatatccag 156060 caatctcaga agcattctgt taagtgaaaa aagccagacacaaaaaggct acatactgca 156120 tgattccatt tgtaggacat gctggaaatg caaaactttgagctagaaat cctatggttg 156180 aggtaaaact tcaggagtta gaaaagggta tgaagaaaccttggaattct gtaacctctt 156240 tgtggtgatg attacatgag tatatgtgtt agtcagaaatgcattgaaat ctatatacaa 156300 aaagcatcat ttactctatg tttattagtt atattaacaaactcaacctt aaaaaacatg 156360 aagtttaatg ctcatttagt aatcagccta attctaaaccaatatgttac ttcaagaaaa 156420 aaaattactt ctatgtaagt taatttttta aggaaaacaatgtatgtggc taccatgaaa 156480 tcataataaa atttaaagac atgaacaaaa acaaggtgaataaacatgtt ataagtgtaa 156540 ataaacataa caaacttatt ttttcaatat aattagaacattctccttca attactggca 156600 ttgtgttttt atgtttcaca cagagcaata gatttgtataggatagcatt ttacataccg 156660 ctgaaattct tttatataga tttagctttc atcagcaagcacatcaaaat tattttatta 156720 aaggtaaatt tttgttttgt ttctaattgc tttttgctgacatatatcag atgtagtgat 156780 ctaagaatat acctggaata tgggataaaa tatttttctttaaaaaattc agcttaagtc 156840 atattaaatt aagcctcaaa tataattttt aatcatcaggtttaattata ttgcaacctg 156900 ttaattttta tctctttgtg ttaagaagtg tgtggaaacaaaagggaaag gagaatctgt 156960 gaacatttga ctgaatagtg aaatataaag atttacttttctgggctctg gtgtatgata 157020 cctaaataat aaggctgcaa agaagatgtt tatcagcctcacatacctga taaggaaaga 157080 tttcaggtga cattttcata ggagttagag agatgataccaagataaaat taagcgattg 157140 ttgtgggagt tagtcacagt tttgaagaca catgttgggagccagaacat tgggaggatg 157200 aagggagaga aaatttggga acagtgatta tgattgtatatgcttataaa ccattatgga 157260 tgtcacagag actttagggt acaagattca taattatatgttaatggaaa aacatgttta 157320 tcaaacaaat cttagtctaa aagaagataa tggagaaggtaagaccattg aatgaagaaa 157380 attcatgaac ctccgggtat aaatgcaaga gagctcatttgtttggctta aaaaaaaaac 157440 agaactgata ttttatgtca tatactatat cttttataatatagattaca aaagtgacat 157500 aatagctatg gtagtgataa aagcatacag tacagttggtaaatttttgg ttgctttggc 157560 actagtacaa ttactgagaa agtaatgcga ataatcactattcagagtta gtgatcaaaa 157620 gcgatgatca tttagaatgt tacacagacc ttgagacaaagcattcagtt cactttagac 157680 cagggtttct caagtggcac caatgacatt ttaggtcagataactctttg ctgggaggct 157740 gttctttgca tcgtatgatg tttagcagca cccctggcctctgccttctt aatgccagta 157800 gcatatccta acacctatcc ccagttgtga tcaccagaaatatttctaga cattgccaaa 157860 tatcccttga gaggcaaaac tgcctaagac tttatgttgatgaaggaaag aaatagtggg 157920 cgcagttaga cacgtgctcc aagcttagaa aatgtaatcaaaagcaataa atattagaag 157980 gttgcagcca tagaaagcaa ggttcttcca cagggagttctcagctgaga ttataaagtt 158040 ttatgaacca gatcagaagt atgaatacat atccagggataaatacaaaa gagtagtata 158100 aatctattaa aaatgtgtta ggctagaaga ctataatgagccacaacttg tgaaaagctc 158160 ttaagccctt tacaagtcat tctctcttct actcccatccttcctggtta gaattaggat 158220 tcaagatgtg tgagaaaagg ataatttcac tacatgagaccattcttgaa atagtttttg 158280 aatgaccagt tactaaagac atgaagaaca agactgtttttcttagaaac tgtagctctt 158340 accatctcat ggatggaaac acacacagat ataccttataatagtagaag tttgtagaga 158400 atgtaatgat acacagatct tgaatggggt aaagaaaggcttccaaaaga aagtaatatt 158460 tgagtcttaa aacatgggct agatttttca atggaggaaggacagttctg agagggagga 158520 ccatttaaat cacagagtct tccaaaagta tgacatctttgaggaaccag gagtttggtg 158580 taaattgaac ttggtttata atgagagaga aagggtcgggaggacggact gcaggggatg 158640 agactgaaat ggcaggctgg ggcttttggg ttaatgggttggatattaaa aaaaattact 158700 tagttgcttg ttttttcttt tcaaattctc atgagttttaaaaacttaga taaagaaatt 158760 gaagtaagtt caaggaagag gagaaagttt gagtgttttaaatacctcta aatcaaggca 158820 gtttacacct attgaattaa catgctgagg tgattgaagatctttagaga aactttggag 158880 aatgatggag ttcccaaaaa actaaagata ggtaaatggtctggttataa taaaccaaag 158940 aaaagactga taagtgcaga tttctgaaca gtggcagtcttcatacagag gcttgacttt 159000 ccctcttttt ccaacacaac tattgtttaa gcttcttcattttcatttaa atgatcacaa 159060 aaatcttcca ttcagtctca ataatacatc tacttttgcctatgtacagt tacctacgtt 159120 gctgccagag gattcttttt gaatagtaaa tttaagtcaaattccattcc cagctttttg 159180 gtggattttc taaacttgat gctatctaag cttctaaattacattcttaa ctgtacacct 159240 ctcctccttg tttccttaca catcagctac acgctgacatttatttcctg gaaagtacca 159300 tgacacctcc ataagacttt tcagatgttg tttttctctttctagaattc tttccaaccc 159360 acccaactca aagcagtcat cctttaaatt tcaaataagccttcattttt gttccctaac 159420 ctcttggtta tctaggcttc atggatctga ccttctgagcatgtacttct atggcatctt 159480 gtatacttat cacaggttat atttatttgt atgactatttaattggtacc tgctgtggtt 159540 tgaatgcccc caccaaaact cattaaagtt taattgccagtgctagtagg tggggacgtt 159600 aagaagtgat taggtcatgt gggttccacc cccatgaatggattaatgct gttattgtgg 159660 gaaggggtta taaaagtgag ctcttgtgca tgtatgctcttactcttcca cttgtctacc 159720 attgaatgac ccttgccaga tgcccttttt tcttttataaattacccatt ctgtggtatt 159780 ctgttatagc agcaaaaaac agactaagac agtatccattctcctttttc tccttctcct 159840 gtcactagat tttaagtgcc atgagagcag gggcatttatgtttttactg ccaaacctga 159900 cagcatagta agtacttgtt atatatgagc tcagtgcattagacgttgga tactgacttg 159960 ttagagatga tctagaaagt ccaggtggtc tctgactttaaaatttccag ctttatgagg 160020 gtttcattgg aacataactc catcataaat ctaggcacatcactggtgat gttaacgctt 160080 atcatttggt ttaaggtagt gtctggcaag cttctccactagtaagttac cattttcttt 160140 ttccaaacgc tgtggagggg agcgtattcc ctttccatactctcctggag gggagacttt 160200 acacgtatta tttggaattc tgtagggaag atttgccttttctcccccat ttattaattt 160260 agtcattgat ttatataata taaactgcat atatttatttcatactttgg gttgtaatcc 160320 agtagtgtgt tatttannnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 160380 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnntgtc 160440 catgttgtgt gaagtagttg ctttttcctt ttttcccctaaattgcagag caaccttcta 160500 ttgaatggat ataccttggt ttgtttgttc attcctctgttgaagaacat attcgttatt 160560 tcctgtttgg ggcaattatg aataaagctg ccgtaaatattcgtgtacaa gtttttgcat 160620 gaatttaggt tttttttttt tttttttttt tgagatggagtttcgctctt ctcgcccagg 160680 ctggagtgca atggcacaat ctcagctcat ggcaacctccacctcccagg ttcaagcagt 160740 tctcctgcct cagcctccct agtagatggg attacaggcatgcgccacaa tgcccggcta 160800 attttgtatt tttagtaaag acagggtttc tccatgttggtcaggctgat cttaaattcc 160860 cgacctcagg tgctctgtct gcctcggcct cccaaagtgctggaattaca ggcgtgagcc 160920 accgtgccgg ccatgaattt agtttgaaat tcacttgggcagatatgtaa atacacagat 160980 gtagcattgc taggttgttt gctaagaaca ggtttaacttcttaagaaac tgccaaactg 161040 tcttccaaag tggctatacc attttattat acattcccaccaacaatgaa tgagagtttc 161100 tgttgttgtg catccttgcc gcatttgcta ctgtcaattttcatttgcat aatgtctttc 161160 atagagcaaa aattttaatt ttgatgaaat ctaacttatatttctgcatt gattgtgctt 161220 tttgtgtcat ataaaaatca ttgccaaact caagattgattttttaaagc aactttatta 161280 agttatcatt tacatgctat aaaattcacc cgtttgaagtatacaattca gtgagctttt 161340 tgggtgtatg tgtattttca gagttgtgca accattaccacagttgcttt tacaacattt 161400 tcatcacttc gtaaggtaac ctcataccca ttaacagtcagtctccattt ctctccaggc 161460 ttccagccct agacaaccac taatctattt tctgtctctatagatttgcc gactctatag 161520 agacatttcc tatagaagga atcatcaata aaatggtcttttgtgactgc cttctttggc 161580 ttagcatgtt ttcattgttc atagtgttat accatgtgtcagtatttcat ttttaattgc 161640 cagtaatatt ccattgtatg gatataccac attttgtttatctgcttatc agctgatgga 161700 catttgtatt gattctcttt tggcttttat gaataatgctgctatgaaca tttgtgtaga 161760 agtttttgtg tagacatatg tttttattgt tcttgcgtagatacctagaa gtggaattgg 161820 tgggtcatat ggtaattcta tatttaacat tttgagaaactcaagatgga tttttaaata 161880 tatactttta ttactttgga gtaaatatat atggcttttctgttgttcct gaatagataa 161940 atgcagcatg tgtttatctc catttgtata taatgtcattaagtgttttt aatatttcag 162000 tagcatcgag cacaagtata atctctatcc ttaaggattgtggggacata caacgtaata 162060 gaagaaacag acctatagaa acaattccag ttcagattgttaggtgaaat atagggaaat 162120 aatgtcccag agaagggagt cataccttgt gcttgagagggaatcaggaa tgtctaaaga 162180 ggagataaaa gattagatct tgtttaatag taacttggtcagataaggtg gtgggggaag 162240 tcattttagg ctagtgataa catctggaaa ggcacagagagatgaagcag tatgggctta 162300 tatagataat tgcaaataac taggcattgc tagagcatggttgtggggag tatcttatct 162360 agaactataa gactggacag tcactaatga tatatatatatttttttgcc atgttgagga 162420 gtttgaattt aggagtctgg attttttata tttattttttgttgatctgt ttatcaaact 162480 ggattactct gtgtgtatgt gggtgggtgg atgcgtgcctatgtgcctat gcagtcaaaa 162540 tgtagcacat cttcctagag tgtgatttta tttgaacatttggacatgaa ataattttct 162600 taattaaaac agtgctggtt gcagtggctc acatctgtaatctcagcact ttcggaggcc 162660 gaggcaggta gatcacttga gcccaggagt tcgagatcggcctgggcaac atggcaaaac 162720 tgtgtctctg caaaaataca aaaattagtt gtgcatggtgttgtgtgcct atagtcccgg 162780 ctacttggga ggctgcggtg ggagaatcgc ttgaactccgaggtggaggt tgcagtgagc 162840 tgagattgca tgacagcgtg agaccctgtc tcaaggaaaaagaaaagaaa aatcagattt 162900 ttatggaatt gaatataaca ttcttgtgaa tttttcaaataagtttaatt tttgagcttt 162960 ttagggctgc tttcactact ctaaaagtat gcaatgtatactgggctttt ttaggcagag 163020 tagcagtatt ctggtcaaac agtacaaggt acataatgtttattatgata ttttacctat 163080 tgtgacattt aaaaaaactt tgaacatttt tgaaattagaatgtatctta gaatctctag 163140 tggtacataa agtaatggta cgttttataa tctaggatcttagggtaggc aaaatacatc 163200 ttttgttttt ttcattagaa aagcaacaaa gggtcattataaaaaactta aaatatatag 163260 aaaatgtggc agaataatct gtagtctcac caaactcagagatgactgca gttattgttt 163320 tggcatattt tcttctggcc tttaaaaaaa tccatatctgtgtatcagta tataattatg 163380 tttttagaaa atcaagttca tatactgtat acagtttgttctatttcttt gcacaacatt 163440 atatgctgaa cataattcta tgtaattaaa tattcaacacaattttaaat gttacctaat 163500 actctatgta tatgaaatta tttgctcttt aaaattgtaaaaactacttt attgtagata 163560 tcttggtccc acattgtatc tcatttcctt aggataaattcctagaagta aaatttactg 163620 ggtataaagt tgatttttat ttatttttaa tttaatttaatttttttctt gatacagagt 163680 ctcggtcagt cacccaggct ggagtgcagt ggcccaaactcggcttactg caacctccac 163740 ctcttgagtt caagtgattc ttatgcctca gcctcctgattagctgggat tacaggcgcc 163800 caccaccgtg ccggctaatt tttgtgtttt tagtagagacggggttttgc catgttggcc 163860 aggctggtct tgaactcctg acctcaagca atccgcctgccttggcctcc tgaagtgctg 163920 ggattatagg cgtgagtcac agcgcctggc caatttttaaaacagtatta ggaaaaatca 163980 tccatttgct ttcttgaaaa ttagattgaa gagtttatatatgattgaaa tgcttttgaa 164040 tttttggtaa cccagaagct gtaagttagc ctttttgcactggccctttt gctaacttgt 164100 gatcatttgg agaaagatta actctaaacg actagctctaatgattagct agttagtggt 164160 tgtcaagact gtggttccta tttatgactc aatttgctcattttatttct tgctgaatat 164220 gttgcatagt agtatttcac ttctctgaat cttagctttctgccagcatc acctgactca 164280 atatagttga caatcagaaa ttgctaaaag agtctagatggtcacaacat cctatttgga 164340 ggttttagaa tgaaggaaga cttaccagat ttgtgattttgcaaatatta ttttgcataa 164400 aactaatttt gtgaataaca aagaggtcaa aatctgccataggaaaagat acaacagttt 164460 aagaaggatc ggaagttctg agaagtttgc tgttttaggtaaggtggcca gggtaggcct 164520 tgttaaaagg tgacatttga aaaaagagtt gaagtagttgagggagttag ccatgtggat 164580 gtctgaggga agagagtggc atacagagga gcagctagtgcaaaggcctg aagggaggag 164640 agtgtacctg gcctgtttga agaacatcaa ggaagccagtgtggctggag aggattaggc 164700 aagggggaga attgtaggat gatatcatag aggtaaggaaatggggaggc taggtcatgt 164760 agagtcttgt aggccattgt aaaatcttgg gcttttgctctgggtgagat ggcaagacat 164820 tagagacttt tgagcagaga agtgacatga tctgacttgtgttttaagaa catcacccta 164880 tgttgagaat agacacaggg caatcagtta agagtctatcacagtaatcc aaggaagaga 164940 tgatggtgac ttggtctagc atggtagcaa tgtagagggtgagaagctat cagattacag 165000 atatatttca aagagagagc caacatattt tcctgaaagataggatgtgg agtgtaagaa 165060 aaaaaggagt caagaatgcc ccctaggttt ttgtcctgagcaaaattgag ccatcggcca 165120 aagtgtggga cacataggta taagttttgg gtgagaatatttcttcagtt tttggcacat 165180 taaatttgag atgtttttca aaagaggata tattttgaaagtaattgaat agataagttt 165240 gaagtttaag agagacatgt agactagaga taaaaatttggaagttgata tgtagatggt 165300 ttttaaagcc caccagagaa gtgagtgtag atagagaagaagtcaccatt gagggctgag 165360 ccttggaatg ctccagtgtt aagaaatctg agagaaggggaggaaccagc caaggcaact 165420 gaaaactact aaggttgaaa agtaaaatct tcagtattgttggaagaatg ttgtgtcctg 165480 aaagtcaagt aaagaaagtg tatcaggaga gagtgattgaagtgttaaag ccactgatat 165540 atcaagatga gggctgagaa ttggtcattg gatttagcaacatgatgctc atttgtgact 165600 ttgacaagta gttttgaaag aaaggtaaga gccgacctgaagtggatttt agagcaaata 165660 gtaggaaaga tttagtcaca gaaattatag acaacttttgagaaattttg ctgcagtggg 165720 gagttgagta atgggacagt agctgctgag agaaatgagatcaagagttt ttttttgttt 165780 ccctaagatg ggagagaaaa cagcatggtg ggatccggttaggaggggta aagcttagta 165840 atagaggaga aaggggagaa ttgcttgagc aatgtccttgaactacagta atgaaaaagg 165900 atgacatcta gtgcatgagt ggagggttta agttaagatagaagtagtct cccatagtaa 165960 aatatgaaaa gacaaaatat atgcttgtac atgctgatagttaagtgtgt aagtggtggt 166020 aagtcagtgg tagttttctt ctgattgttt cgttcgtttcagggtagtag ccaaacaaga 166080 tcattagctg tgaacgagga tcagtgggag agctgtgggaggtgtgtgtg aagaaagaag 166140 aaaacttgaa atacgtctct aggagagtgg gagagtgactagaaatttag gattgccaag 166200 tagaactaag ggccagcttg atgtttgtga ttatgaattttaaaatagga tcagtcattg 166260 tgattttatg tatttattga gacatattca actgcatgggtgctgacata gagtagttgg 166320 agagttggat ttaactataa taggattgtc gttttgccaaactaagtaaa gtgaggcaga 166380 agagagctca tgacattaaa ggtatatgca agggagtgattataataatt gaccatgtaa 166440 tttaagctgg ctaggtagga aaaagaggct atcagtgcaacgagggaaag tatgagtgta 166500 gtagaatcaa tggattggag gtcctggtgg ggttggggttgttggagtta agatgctaga 166560 ggaactaagc aggaaagcta taagagagtg cttagagagtgtaagttttc taattgaaat 166620 tgttggaaga ttataattat gagtaatgac aaggtctagtatatgaccat ggaaatgagt 166680 gaccaaggta aggtagagga caagatcatt gaaggagagaagtataggtg ttgagaggcc 166740 aaggtactgt aagtattatc tgtgggtata ttaaagttattaagaattaa aacaggagaa 166800 tattggagag aagttcagta aatcaggaag taaaactgtctagcgatgag acgtggtgac 166860 ctctgagagt cagtagatta cagcaacaat gagaggtgggaaatacaacc taatataatg 166920 aaaatcaaag ctagcagatt taggggtggg gatgtgggggatctcccact ttcagggtcg 166980 gttgtctaag gcatgtggaa aagtaaaggt ccccacttgagagctgtggg gaagcagtgc 167040 cctcagaagg ccactgggtt ttattagagc aggagttggtgaactacagc ccacaggcca 167100 aatctcaccc accactgtgg ctcatgaact aagcatgttttttgtatttt ttcatgattg 167160 aaaaaaaagt caaaagaata tataattaaa gaatatacaattgcatatat aagtttgaag 167220 tttaagagag actagactag agataaaaat ttggaagttgatatgtagat ggtttttaaa 167280 gcccatcatg atcatgatgg tgaaataatg aaacaatatcatgaatgtga aataatagga 167340 aattcagatt tcagtgtcca taaataaagt tttcttgggacgttgctaca ctttaaaaaa 167400 tgtattgtgt atagctgctt ttgttctaca acacctaagctgagtagcta caacagcgac 167460 catatgtgat gctctccttg cttcacactg ctgctcagcacagtagaaat cacagtgatg 167520 ctgttacagc ttgacagttt tgagtgctat atgtatcaccatatcacaac attttatttg 167580 ttttctatta ccagtacata cgcatgtgtc aaagcaagaagagaagagta tacttgaaat 167640 gttgtctttt tttttccctc ccctaaaaga ctgtcacacaggcaggagtg cagtggcaca 167700 ttcatagctc actgcagcct tgaactccta ggctcaagcagtccttctgc ctcagcctcc 167760 caagtagctg ggactacaag tgcatgccac catgggcctggctaattttt aaattttttg 167820 taaagacaag tcttggtatg ttgtccaggc tggtttcaaactcctggcct tgagcaatcc 167880 tcctgcctct gtgtcccaaa gcactgggat tacaggtgtgagccatcatg cccagcccaa 167940 atgttgtcct ttttgaagca taatggagaa tggattatttttatcaagtt aggtggcaaa 168000 gcattttttt aaattatgca atgacagtat agctgtgctaaaggaataca gtgtactttg 168060 gacattccca gactaagcac tcactatgat cttcctgactcataggacag caacagtgag 168120 aaaaaataga aaatttaaaa cagagtatct catcccagtaaaatttcttc ataaagaaaa 168180 atgttcatgc aggcttgacc aaaaaaaaaa aagaggctgcaatgttatgt aagtgtatga 168240 gtggtttatt cactagccta gcagagaaag ctgttcactgatggtgtgtg attgcagcag 168300 ccaaacaaat gtgtacagag agaaaaactt gcctaagactgttagccttt gggcaagaat 168360 agctgctcaa agaattgggg gtattgggag tggcatcaatagtcaattaa aaaataacca 168420 attttgagtt gttttccttg actcttgttt atttgaggaatcagtgcaga gtttgaagtg 168480 actgaagaat tagcctgtgt atatagtctc cccttacagacttgggaaag cttggtctta 168540 ttttctttag aatatgtatc acaatgtgaa attaagttacatatttattt atgtacttat 168600 tatttttctt tagagcagca gtccccaaac tttttggcaccagggaccag ttttgtggaa 168660 gacagtgttt ctatggacag gatgggggtt ggggggtggcagacgggatg gttttatgaa 168720 actattccac ctcagatcat caggcattaa aaaaagattctcataaggaa tgtgcaacct 168780 ggatccctcg catgtgcagt tcacaatagg gtttgtgcccctgtgagaat ctaatgccgc 168840 tgctgatctg acaggaggtg gagctcagat ggtaatgcttgcctacccac tgctcacctc 168900 atgctgtgtg gcccagttcc taataggcca tgaaccggtacaggtccacg gctcaggggt 168960 tgggaatccc tgttctagtg catgcattgt tttctgtctcaatggagcca taagcctaca 169020 aagttcaggc tatatagtac ccctagacca agagtgggtttcttacatga ctactttaca 169080 ataaataggc ttcgtttaag atcctgagat ttgggtaggtgtgaatacat agtcctgagt 169140 gtttgggaaa ctaaagtcca atgcttagaa ttttaccattaagttcttta ttagttctag 169200 gttggaccct tggtgagatg aactaggaaa agtagaaccatagaatctct gagtttgatt 169260 gaaagccatc caacagaggc atttacactt gttagcacgtaaacctgtat tagaacaagc 169320 tagaatccaa ggcagtagaa catagaaaga tcttttagatttcaaatgac ttgtctgcgg 169380 tctttgtcaa gactctacag cctattctag ctcatagctagagccaagaa tctttttttc 169440 aacgtaacag gctattatat tatccaggtt cagaactttctctctcatct ggggtattac 169500 aatactctta aaattctccc cacctcctgt cttgactttattgaactcat cttctacgta 169560 tttcctagag tagttttcct gaaatgtaag tttgactatgttgttttatc catcataatt 169620 ccttttcatg gcttcctgcc catagtagtg gtgcttctcagttcccagca gattccagag 169680 cattcctcag ggactgtatt atggaagagc ctgaagtagatgaggctctg tctcttctct 169740 gacaccttta acctcattta gaacagagca gtttctttgatctatttcat gtagagagct 169800 actttaaact ttaatgtgaa aacggttttc ggtacttaaatattagtttt attactgtaa 169860 ctactggctg ataggtaaag ttcaagctcc tgaacttacctggggctctc cagcctcttc 169920 tgtcagtcct tactacaaac ttgatactcc agcaacatagaacttagata cagttcctct 169980 gtgcttggtt tacattgctt gtctgcttat attgttcacttgttctttcc tttttttgag 170040 acagagtttc gctctgtcgc ccagactgga gtgcactggtgtgatcttgg ctcactgcaa 170100 ccttcacgtc ctgggcttag gtgatcctcc cacctcagcctcctgagtag ctgggactac 170160 aggaaactgc cactgcaccc agctaatttt taaaaaaatattttttgtag agacagggtt 170220 tcatcatgtt tcccaggctg gtcttgaact cctggactcatacattccgc ttgcctcagc 170280 ttcccaaagt tttgggatta caggcatgag ccaccacacctggcctgttc tttctttcat 170340 tctctatcat ctagctaacc gctactattc agtttagacttttttcaggt accatcattt 170400 acagaaagtc ttcctagcta tctctcaccc agcaggctttagttaggtgt cctccttagt 170460 actcaaaaaa ctgtttatct tccttattac attttttgaattcttttttt cattgtttct 170520 gttttatttg ctagagtttg aactcttaaa gaataggaactacattttaa agttatgttt 170580 atttatctcc agtgctgtac agcctgatgt atggtagatagaaatatttg tgcagcgaat 170640 taattgatta ttgttcatct ttctggtgca tcttgcagtggatatgtaac ctaaaattaa 170700 aggtacttat aactttgtag aaatttgctc tctgttacatgtatttattg gtcataaagt 170760 acacaaacac ttgagtttaa atgcatttga aaaggaaaacaagaatatta atacagctct 170820 tattaggaac tggaactgaa gtttaatagc tgctgtgtattttgtacaac tctgaaatgt 170880 gaagcttttc catgtaatct catgccagtg ttgacccatgtggattaaaa tagccctatt 170940 ttaatctatg accaaattag gaattagaat atctatccagtggggatttg tgaattattg 171000 tagtttgtag tatgacaagt ttatataaat aattgcagtgattttcactg tgtgtttcaa 171060 gttaaaatct aggttcaaat agagaaatgt aatgctataagtattaaaga ttaccagttg 171120 gaaaatttac tgtttttttt ttttttagtg tgtttgcctttgatatctta aattacatct 171180 ttttttaaag gatcactaaa actgacctta taaaaagaaaaatttattcc agtattaatc 171240 taaaatatct actcatgttt cctaaattca gatttgatgtgtacttaaaa taccaggaac 171300 taattagcta attattttca gcttttgttt gtagtgtaatatttgttttt aaaacattct 171360 gttaagtcaa attttgatta ataaaaacct ttagtagttgtaatgaagtt attctggaat 171420 ttactttaaa aaatcttagc aaattgaaca tttagctactttatagcttc aagtttcacc 171480 atattttcaa ataattcttc atgaagtttg ttgtttttcattgtgtttct taagaactcg 171540 aatgtaaaat aagtagtgct agttgtaatg ctttagtgatttaaaatgat aaacttttct 171600 tttcatacag ataaagacac aataaataaa atcagagatttacgaatgaa agctgaagat 171660 tatgaagtag tgaaggtgat tggtagaggt gcatttggagaagttcaatt ggtaggttat 171720 tttaatgaga atacaaaagg actttattaa attaaaataatccacttgtg tgtttaacat 171780 attatttggg aaaatactgt ttttatagaa ttgttgctttaagttccttt agacattgtt 171840 gcctgagggt atggaagcct agtgtcaaat ctagccatagtgggaagtgt tttccccaag 171900 tatctgtggc agaaaagaag tgtcaggcat cagaagataaatctttgctc ctaatattag 171960 actaaaatgc tgtagaacag taagctttat aaacaggaagcagcttacat agccgtgtgg 172020 aggggggaaa gtgtcttgta atataggaaa taaaaatttttttaaaacat tacttctgat 172080 aattaaagtc ttgacattag ctacttggaa taatttggcagaagagaaga aataaaaata 172140 actgaaacag taaggctaaa ttatttagca agtattaaaagcacttagta aattgttctg 172200 tgaaaccttg agctttaaat gttaacaaaa ggttcattattttccctttt caattttaaa 172260 ggtaaggcat aaatccacca ggaaggtata tgctatgaagcttctcagca aatttgaaat 172320 gataaagaga tctgattctg cttttttctg ggaagaaagggacatcatgg cttttgccaa 172380 cagtccttgg gttgttcagg tatgactttg ctactctttcattattgctg cattagtttg 172440 taactaaaca tgtctgagaa gcttagtttg tgttttagaatagtagtaga gaatttactg 172500 gcagttacaa aattgtctaa ttacctttta aatcttaaaatttaattgta ttgaatcatt 172560 tctagcaaga aaggctagaa caaaataacc atgcttctgtagaatttata tataggaaat 172620 ttaatttaaa tgtagaattt gtgctgggcg tgatggctcatgcctgttat ctgaatactt 172680 tgggaggaca aggcgggagg atagcttgag cccaggggtttgagaccagc ctggacaaca 172740 tagtgacacc tatctctaca aaaaattaaa aaaattattgaggcatggtg gtacacgcct 172800 gtggttccag ctactgggga ggctgaagca ggaggattacttgagtccag gaggtcgagg 172860 ctatagtgag ccgtgttcac atcactgcac tccagcctgggcaacagagt gagaccctgt 172920 ctcaaaaata aaaataataa atataaaatt taaatacagaattttaaatt agatattttt 172980 aaatacagaa gatatagaaa acttaatcta cttagatttgagggaagtcg tatattatta 173040 aaatttaaat agatactttt agttaatatt gaatttttttccccaaacat gaggctctca 173100 ttttgtttag ctgtctcctc atttatctgt ctcttattttgtgaagagga gataataaga 173160 gaaatgaagg gaaagtgaga ggaaaccaag ttaagctaggtcaccaaata atacttgtct 173220 ttcggttttc agtggaaaga aaaactagaa gtcagtagttttaggtcctc gtcttgattg 173280 ccataggcct ttctctgaag ctgtggccat gttacttaacatctctgggc cacaatttcc 173340 ttgttcataa gtctgaaagt tgtctttgat tggttatgttcaattgtgga ggtaccttag 173400 gcaaaatcat ggggtcaagt ggggttttgg gtagattataattctgagtt ttctctctgc 173460 ttttatgagt tcagcttcat gttttatctg tcttatgtattgggatcctt aatgcaaaat 173520 gtcatacggg ggaagttaaa ctgggctaga ccatttctaattttctaaca tgtttttgtg 173580 atttatttat aatatataat gaagtctgtc cttaaaaagcataccagtaa ttggaattaa 173640 aaggatatga aaaatgttga aggtttatgc catagcactttttttttttt tttgagacgg 173700 agtctcgctc tgtcgcccag gccggagtgc aatggtgcgatctcggctca ctgcaagcaa 173760 gctctgcccc ctgagttcac gccattctcc tgcctcagcctcccgagtag ctgggacgac 173820 aggtgcctgc caccacgccc ggctattatt ttttgtatttttagtagaga cgggtttcac 173880 tgtgttagcc agcatggtct cgatctcctg acctcgtgatccgcctgcct cggcctccca 173940 aagtgctggg attacaggtg tgagccaccg tgcccggtcaccatagtact tttaaacttg 174000 aaatgtctat gtttttacca actatgtaga tcagagtttctcaggcttaa tactattgac 174060 attttgggcc agataattct ttgttgttgg gggctatcctttatattatt tcagttacta 174120 atgtgtttat ttaggatgtt tggcagcatt cctggcctctacacagggga tgctagtagt 174180 gactcttccc acctcacaca cagttgtgac aaccaaaaatgttttcacca ctagttaaca 174240 accactgata catgaacgtt cagtgtctgt tagtgaaaaaatatgtgaca taattgttac 174300 tttcagggct atttacaatt taggtattca gagctttatttctctggata tattgtctat 174360 aaatagggtg aaactgtcac caaattaaca gtatttctttatggggccca aatgacaaat 174420 tatatacttt aatctgggat agtcatgaaa tgccactttgtttcttatcc ttctctcttc 174480 ccccaaaata cattttttac attaaaataa ctgagtctgggccgggtgtg gtggccccca 174540 tgcttgtaat ctgagcactt tgagaggctg agacaggaagatcacttgag ggcaggagtt 174600 caagactggc tcccaatctc tacaaaaaaa caaaaaatcaaaacaaagta actgaatcta 174660 taactaaaat tattaaacac agttgaaaaa aatgaaacattaagaagatt ttaagacaaa 174720 catctgggta aattagagat catcttctct agggcttgttttttttagtt taattctaaa 174780 cttcttgtat ttacttaaaa tgttatttta taaatttgcccttcttttta tttgatcccc 174840 agtcacataa tatatttatg cctgatatcc tcgctaatttcacattagtg gattttgctg 174900 ttactatttg aaccctagct gctgccttcc tcctacttctgtgttagcta ttactgctcc 174960 tcctcctgac tgctgtcatt tgttgagcat ctgttgcactaagtgctttc tatattcagt 175020 aatctttttt aacaaccctc aaccctgaaa gacagttaatctgttaggtg cactgttact 175080 ttatggatta gtttatgatt tggttcatta agcctttattaagcaattgc taaccgccag 175140 gcatctggat acttgactaa gcagcaggta taaaagttaaacgaagtata gtccttacag 175200 tgttttagaa gagattatag tctcatagac tcgggtaggttaagcaaatt actagtcaca 175260 cagctaataa gaaacagctg aggccgggcg cagtggctcatgcctgtaat cccagcattt 175320 tgggaggcca gggtgggcgg atcacgaggt caggagatcgagaccatcct ggctaacacg 175380 gtgaaacccc atctctacta aaaatgcaaa aaattagccgggcgtggtag cgggtgcctg 175440 tagtcccagc tcctcgggag gctgaggtag gagaatggcgtgaacctggg agttttagct 175500 tgcactcccc cccccctcac gccactgcac tccagcctgggcgacagagc gagactccgt 175560 ctccaaaaaa aaaaaaaaaa aaaaaaaaaa aaagaaacagctgaaactgt aagccaaatt 175620 tatcaaagac ttcttgagca ttgtgatatt gatataaatttaatagtaga ccccactgat 175680 gcacaagtta aattagaaat acagtatttc tttgcctaggagatttgtat atttactgaa 175740 aatattacag tttaaaaaaa ttctcagtaa aagaaattcaaatagtgtag aaaattatga 175800 agaaaggata tctccatgta actcttctga ataattatgttatagtcatt ttgcatatac 175860 aagggtcttt gtggcattta tgtttgtctt tttttaaaactagacttgca acttctgtaa 175920 tttgattatt ttacttaaat cttttattaa catttttgccactttttaag tgaaacaatt 175980 ttgtttaaca ttaatgaatg tgttttgttt gttttttctatttgtatagc ttttttatgc 176040 attccaagat gatcgttatc tctacatggt gatggaatacatgcctggtg gagatcttgt 176100 aaacttaatg agcaactatg atgtgcctga aaaatgggcacgattctata ctgcagaagt 176160 agttcttgca ttggatgcaa tccattccat gggttttattcacaggtaaa gataaaagtg 176220 ctttaaattt tctcatttgt tgagggaaat agctaaaaataactttaaca tttcttactc 176280 tcagttcacc cattcttctt ccgtatgaca taagtgacctttcagaactc aagactctgg 176340 ccaggcgctg cctcacgcct gtaatcccag agctttgggaggccgaggca ggcagatctc 176400 ttgagctcag gagttttgac accagcctgg acaacatggtgaaacccatc tctacaaaaa 176460 aaaaaaaaaa aacacaggtg tggtggcatg cacctgtggtcccagctcct tgggaggcta 176520 aggtgggagg atcgcttgag cctgggagca gaggtttcagtgagctgaga tctagtcact 176580 gcacttcaga ctgggtgaca gagtgagatt ctatgtcaggaaggggggaa aaaaagaact 176640 ccaaactttg aagtttataa ttttagcatt tcaatttaatgttgatataa atgtattctt 176700 taatatccct taatctgatc atatagcctt taaatggtgttctgaaaagt aaaacttgta 176760 aactagtttt ctaaggtatg tgccaggagt taaacactttgatgttaatt ttttaaatat 176820 gtaacttaat ttgcttttaa ttagtgaata ggtcgtatttattcattaga cgaatagttt 176880 catttgttgt acaaacactg cttgaatcct gtgtgcagttgctgtgccta catgtcaggg 176940 atcaaaaatg aaagtgactt agaccctgcc gtggggtttttttatgtact tacctatcta 177000 atatgtttca atcacctttt atgtctgagt gttgtatttttgatggtctg tctctctcaa 177060 atgatttgat tttccctctg aaagaatgaa acttttaaaaaaggaatata tatgttttct 177120 tctgaggcat taacatataa agaagtttct ttgatgaaatttaatttata gatgtggata 177180 gagaacacat acagatcaaa gatggcctta cctagattgaggatgcttga cctaaaataa 177240 ctgcctcata taatttttac agtgtctaat ctgtttcagaaaatactgta ctattttttc 177300 cctcctgcag agatgtgaag cctgataaca tgctgctggataaatctgga catttgaagt 177360 tagcagattt tggtacttgt atgaagatga ataaggttaattaactagat tttaatttag 177420 tttttgctaa ttcaaggtag atgcttcttt agaaaatggtttaagaattc tttagaaaat 177480 ggtgtaagaa tttactaacc atagctggac ttgtccagaattaatatgac cttatttgtt 177540 gaactagata ccatggtttc aatgttgtat tagtaacttgttagtgattt attttttcct 177600 ctctatttag ggtaaatatg gattgccaga tttactaaatccttcataac aactctaata 177660 tacactactg gtaactagga atggaattag cttaaatgtcactaaatact tatgccagtg 177720 ttaaaattag actttcataa aaattggatt ccagtaatgtgtgtattttt tagtgtttta 177780 cttcagttaa catataaaag attttactgc ttattttaggagtgagtctt aaaagatacc 177840 tatcctcact gtttatataa taaataacat atacacagctgtatatgtat atacatatta 177900 tgtatatgta tatacatata cattatgtat atgtatatacatataataaa taacataaat 177960 aaatatgtta tttatataat aaataacata tacacagctgaaggagctga aggagctgtg 178020 tatatgttat tccttcactt catatacaca gtgaaggaaacactgcatga gaacttctgt 178080 atgaacattt agttataatg ctgtttcatt aataccagcgaatataggta acagctaaca 178140 agtctctcag gtttgttggg ggagggcagt taaaattcacatcaaataga cattcagtgt 178200 gctttacact attatgccct tttcccatac atatgcttccccagtttgta tttgtacagt 178260 cagcttttgt atgtttcttt tgtagtttca aacatagttaaatagctatt ttagtagaaa 178320 ataatgaagt cagtgtttat accaatatgt tgtaaaatgccctatcctat gaactcccca 178380 gtgtatttgc actggcctgt gtacttgttc tcagcagttaaatggtttca tgatattggc 178440 acaacttggg gaataataaa tgtggcagta tgtttttctagtgagcatta ttacaaaata 178500 atcactggtg tgtgtatata tgtgaataca catataatgtgtgtgtatat gcattcacat 178560 gtatacacat acatacatta tatggtatat atgtatatggtgtgtatata gtatgtatat 178620 atatgattaa gctgtgttaa tttttgtcag gtttccattcagttttaata tatgatattt 178680 tatagctcaa agatttggag tcccataggt aatattaataagaaagtcat tttaaataat 178740 tgagacttta tatcctaatt caaaatttga cctgccgcgctttttaggaa ggcatggtac 178800 gatgtgatac agcggttgga acacctgatt atatttcccctgaagtatta aaatcccaag 178860 gtggtgatgg ttattatgga agagaatgtg actggtggtcggttggggta tttttatacg 178920 aaatgcttgt aggtgagtaa ggaagatttt atgtctctctattacagttg ttcatctcaa 178980 aacttgccac ttggctcttt ctaatatatt tattatatgaagcaatcttg attttatttg 179040 tgggtaataa acatgatcca aacctgcttc gtatgtgtttttaaaagcat ttagaggctt 179100 agagcaactt tgtgatgtgt tcttttatat attaggtgtatgtttttagt ttaatacata 179160 cctaataatc tttttaaacg ggtaactttg actcttcctttgaaactgac tttttaaaaa 179220 tgtatttcat tttgcttttg ttttctttat caatttttcaggtgatacac ctttttatgc 179280 agattctttg gttggaactt acagtaaaat tatgaaccataaaaattcac ttacctttcc 179340 tgatgataat gacatatcaa aagaagcaaa aaaccttatttgtgccttcc ttactgacag 179400 gtaaataatt ttaaaactga gcctgtatta attctgaggacttctcatct tctgctccat 179460 ttttaaggtc catatctgag cttctacatg taaattgttagaatataaat gacagtgaaa 179520 cctgatgtaa ttttatgata ttctagatgt cttttgtagatctcaactca cttatcatac 179580 ctaagaaatt gtgataataa tacagtcttg gctgatggctaaactagatg acttttttct 179640 tttattgttc aatttttaac ttggttaatt ataaaaattactgtgtattt tactgaatct 179700 aaaaaaattt tttaacataa catctctgaa attagagtacatctcacagt caatggtatc 179760 agttgggagc atcctttttc atagtggtat aaaaacggtgatacacctta tactttaaac 179820 cattttagat ttgatgatgt atggtatatg gttgttgaaagagtcaatag tataaagcca 179880 tttttaaaag tatactatct tttttcttct agacataatagttggtgttc catcttgtag 179940 atccaacaag cattttaata ataatgtctg tctgcatttttgaaagaaaa atggcatatt 180000 atatatacct ttgtggttta cttttgaaat ttgaaagtgaaccagatgta ttagtaagat 180060 tatatttaat gtttatgatt gtatattgtt aaatattagggagacctagt acttttaaat 180120 atgtttcaag ttgagatttg ctttaacttg tctcccttcattatgtcctt tagagcttga 180180 agtatggttt ttcaaatgtt tacttttttt ttttttttttttttttaaat agagacaggg 180240 ttttgctctg ttgcccaggc tggtttcaaa ctcctgggatcaagtgatcc tcccacttca 180300 gcttcccaaa gtgctgggat tacaggcctg agccacccatgcctggctaa tttttacatt 180360 gattgattga ttgattgaga cagagtctca ctctgttgcccaggctggag tgcagtggca 180420 tgatctcggc tcactgcaac ctcccacctc ccggattcaagtgattctcc tgcctcagcc 180480 tcccaagtag ctgtgactac aggttcctgt caccatgcctggctaatttt tgtatttttg 180540 atagagaggg tttcactatg ttggccaggc tggtcttgaactcctgacct catgatctgc 180600 ctgcgttggc ctcccaaagt gctgggatta caggtgtgagccaccgcacc tggccaattt 180660 ttacatttta atataaagca ttgtattatg ctgtttattaggtgctaggg atatagtagt 180720 gaggaagata cagagccact tttctcaaat acctcatagtcctttggggg aattagataa 180780 gcaaatcaga catttatagt acacacagct aattaccatgagataagtac catgtgccgg 180840 agaaccatgg agaggttttc taatccagtt tgagagttagtgaaagcttc ttggaaaaat 180900 tgtcacatgt ctaagccaac ttatctttta gcttatgcaaaaacttagaa tttagagtat 180960 tttctttatt tataagcact gtactaggga aaaaaggtgctaactttctt tttccaattc 181020 agctaaaatc acatttttaa aaactttttg ctcaccaaaaaagaggctgg tatcagtaac 181080 ttgtccagtg aaagctctga attctgtggc agtatcaagaattagtagtc attggctttt 181140 gaatggtcag aaataattaa tagattataa attggaagttcaaaatttta aatgtgtttc 181200 tggaggtttt tggtagcatc tggtattgct gcctaagtaaagttttggtt ggggaaaaaa 181260 aagcctaact ttaccattgt aagaaaaaaa taacacacacacacaaaaaa ctgtcaagaa 181320 aatgcatggt gctttattca ttttgctaat gaagtaaggtggtgattaat ctggattttt 181380 gttgtcagta gaaaagtcat gggtgttttt tgtagttattctttagtttt cttttttttg 181440 agacggagtc tcgctgtgtt tcatccaggc tggagtgcggtggcgcaatc tcagctcact 181500 gtagcctctg cctcccgggt tcaagcgatt ctcctgtctcagcctcccaa gtagctggga 181560 ctacaggcgt gttccagtac acccgctagt tttatatttttagtagagat ggggtttcac 181620 agtgttggct gggctggtct cgaactcctg acctcaggtgatccacccca cctgagcctc 181680 ccaaagtgct gggattatag gcatgagcca ccatgtccagcctttcttta gttttctttt 181740 aaaaaaattc tttaatgttc tcaatcttta acatattttctatttgggct gatactatgc 181800 tgacaatata atttcacctt agaactatat ttgtcgtcctacactgattt aatatcctgc 181860 ccacaaataa acctggacag gttataataa gatcttaaatgattgattac ttttctcctt 181920 ctgtcattat tcagggaagt gaggttaggg cgaaatggtgtagaagaaat caaacgacat 181980 ctcttcttca aaaatgacca gtgggcttgg gaaacgctccgagacagtaa gttttttttt 182040 ttctttcata ttttagtgaa tatttgaaag tagttgtggtagtttgttga aactacattg 182100 ctaaatattt gtgtgtatct gatgaaaatc agaagccacaaatactatat gggaattgtc 182160 ctaacctatt aatttgataa tattgggcag attatattgttactcctctt atgtcccacg 182220 caggataccc agatgaaata taaatgtaat ggatttaggctcaccagtca taattttaca 182280 tttgatcttt gttgaattgt tcattttagg cgttagttatcttattatgg aggtggtcaa 182340 taagtagtaa gtaatgagat taaaatatat gtttttgtgagaagacaagt tattctcttg 182400 tttttattaa aacccaaaat ataagaaggt tctattattaattccagaat tcaatttaat 182460 tttatttatg agtttatagg atattgtgat atacttgattccatcaggct tttgcagctg 182520 gcaattcaga aacaaaaatg tattgtataa gaaactaattttcagtcagt ttagaacact 182580 ttcttacttt ttaaaaaaag ttaaaattac aagatgattcacagaaaact ctacaacata 182640 gctgaatcaa aattatcatg acaccaaagg ttctcatcatcttcgtggat ttgggaggta 182700 aagaattcat ctttcccctg ctgacatctt aggatttttttccctgttca tctatcagag 182760 aaaggataat gtatctcagt ccctaacttg ctgccttagcaatagcagtg aatagtaaca 182820 atggaaatga actcagctac cttattttag aaagttacacagtagttatt tgtcttaagg 182880 actgcctatc tagttgagac agtgaaggat agtactttgcagagagatgg aaatcttgtt 182940 aattctgatg aaataaatca tgtaggtttg ctaatattagtgaagcattc tagttatcaa 183000 atattttaaa agaacaattg tattgccttc aagtactatagcacagtact tacattgtgc 183060 tgagctataa aattgtctaa ggaggagaaa gggaaaataaatcctctgga aattaagaaa 183120 ttatacaaca aagaacatca ttgaatgacc agctgtctgagaagctgtaa gagcagcttg 183180 actttgggag aattttatta gcaattatat actaggaaataaggaacttc ctcctcttca 183240 gaattaaatg tgacataatt attatcttca tgctgcaagtgaaattgttg catgacaaag 183300 agatgatcag ggagtgagaa atggttacat caaaacactttttgtgtttt tattcaggtt 183360 attctcttag taaagtttaa gaaggctgaa gacgtacaaatattattata aatctgaata 183420 aaactttgaa ggggactgaa gaaaatacaa aataatattttgtattgctt taaattcttt 183480 ttcaaagtta agcaatgcat aaataattat agaataaccacacaataaat caaccgcaaa 183540 agtaacaagg atatttttcc ttttatgtat tgaaaaaccgatcacttgaa agaggaggta 183600 atttagtcaa caactattta ctgaacacct gttgtatattgatcactgtt caatttcaga 183660 cactgggaaa acagcagtga tcaaaccacc aaaagttttctttctccctt ctggagctca 183720 tagtcttatg gaattaggga gtccagatca gtggcattttattatattgc tgtacctcct 183780 tcataaaact acttaagatc tgcattatct atggttttattctagcgtgg ttagatttag 183840 catacttaaa agaattttgg tagtttttcc taattctaagaggtcctgtt ttgtggttat 183900 tggaaaaaac aaagaaaaat tgaaatctgt agaacttattgttctttgct tcccttcccc 183960 tcttcctacc ccttctttac ttcccacccc taaaataccaagaattcttt tattctgaac 184020 tgacctcttt gacctgctaa atttttaaaa cttatgtaacagttataatt ccttttaatt 184080 tttttgggtt tccattttcc gtggcaaaat tgctttctaatacaaataat agtttttttt 184140 ggtattttta ccacttaaca tgtctccaca ttgcaatggcaattaatctt caccttgtgt 184200 tccttggtaa tgtgggagca ggatacaaaa tagcttgatttttggcagaa tttccatgag 184260 cttcctgatc ttgttcacaa agtatgaaga ttttaacactatttttccac tcatttgaga 184320 taaaattgaa ggagtaacat gagtaagatc aaagataggagaaggacaaa agaaatgaaa 184380 agacctaaat aacttactac aggtcataac tgcaagcaaacaacaatgtc aaaattcaga 184440 tctaagtctc ttaactccaa agcctttgat ctttgaagtaccatgtacga tttaaagatc 184500 gttacctgtt cagtgttata tagatcaaaa atggaaggaattgacaattg ttggcagtta 184560 aatttggggg aaaaaaaata acatttatta catatttagcatacattatt aataatactg 184620 ttatatttag tatttaacat agtattaata atttcaaatgaaataaatgg caaaggattt 184680 tgctagttac cacatattta acatattaat attaataatgaatagggcca ggtggctcta 184740 ttgtacagtg ttctacagta ttatacagtg gctcatgcttgtcatctcag cactttggga 184800 ggccaaggtg ggaggacctc ttgaggccag gaaaccaacctgggtaatgt tgcgagaccc 184860 tatctctata aaaataaaaa taaaaaatta gccaggtgtgatggcatgca cctatgtttc 184920 aactacttgg gaggctgagg taagagaatc acctgagcccaggagttcaa gggtgcggtt 184980 agctatgata atgctactgc attccagcct gggcaacagaacaataactg tttctttaga 185040 aaaattaata atactacatt aaatatgtgg taacttgcaaaacactttgc catatattat 185100 tatctcattt gaaataagat attggtgtta cttaatcaacctatagtttg tgggcaacac 185160 acaagatgct aacagtggta atacttctgc cggctcaaaactcattgtgt acacacatat 185220 atattattct aggttctgtg agttaatcaa gactagtaacctttctgttt gaattttaca 185280 ttctcacggg agagattatc aatgtgggct ttgtgaataaagaactgaag gcctagcaag 185340 tttcagggct cttttactta ggagtttaga cgttctgaacattataatgg atttttaaaa 185400 aataagttat ctctttgtat tgtgataaag tatgcataagataaaattcc ccatgttggg 185460 catttttaac tgtacaattc agtggcatta agtacattcacattgtctta aaactattac 185520 ctctactgtg tccagaactt tttcgtcatc ctaaactaaactcttaaata gtaactctcc 185580 atttccctgt ccagtaatca ctattctttc tgtctctatgaattagacta ctactcaggg 185640 tgtttcgttt aagtagatta ctataatatt tgtctttttgtggctggttt atttcaccta 185700 gcacaatgtc ttcaaggttc attcatgtca tagcatgtatcataatattc cattgtatgg 185760 atatactata ttttgtttat ccatttatcc gttaatagacacttaagatt gctatctctt 185820 ttagctattg tgaataacaa acagtagaat acaaatatctgctttccata ttatattacg 185880 gatattttat atccatatta atgttatata tatggatattctgtaatatc cctgttttct 185940 attaatttga gtatatacct agaagtggaa tggctaagtcatatgataat tagttagttt 186000 tgtagagaca gagtcttgct gtgttgacca ggctagtctcaaactccagg gctcaagaga 186060 tcctcctgcc tcagcctccc aaagtgcagg gattgtaggcatgagccacc atgctcagcc 186120 atatgtttaa tgttttgagg aaatgccata acattcacaacatttgcacc atttcacatt 186180 cccagcagca gtgcaccaaa gtgctgggat tgcagacgtgagccaccacg ctcagccata 186240 tgtttaattt ttttgaggaa acgccataat agtatccacaatgtttgcac cgtttcacat 186300 tcccaacggc agtgcacatg ggtctggttt ctccacatctccagcatttg ttcttttctg 186360 ttttgtttgt ttgttttttg tgtttaaata gtagtcatcctaatggatgt gaagtggtat 186420 ctcattgtgg ctttgattag catttctcta atgaccaggaatggtaaaca tcttgtcatg 186480 ttgcttattg gccattcgta tatcttctct tgagagatttctattcaagt tctttgccca 186540 ttttaaaatt tggtggtttg gtttttttgt tgttgttgagttataggagt tctttaaaaa 186600 ttttggatat taatctctta tcagatacat ggtttgcagatattttctat tctctgcact 186660 ctgttattag tgctttcaag cacaaaattt ttaattttgatgagatctgg tttatttctt 186720 cttttgttgc ttgtatcttt ggcttcgtat ttaagaaatcattgccaaat ccaatgtcat 186780 gaaatgtctt acctatgttt tctttgaatt ttatagtttcagctagtatg tttaggtctt 186840 tgacccattg tgaattaatt tttatatagt gtatagtaagggtccaattt aacatttttg 186900 tatgtggata tccagttttc ctaacactat gtgttgaaaggactgtcctt tccctgcatt 186960 gaaagttgtt ggcaccttgt atcaatcatt aaaccatatacatgagggtt tatttgtggg 187020 gtttttgttg tattccattg atctgtataa gtttgtttttatgttggtac cacactgtat 187080 gattatggta gctctgtagt aagttttgaa atcaggaaatgtgagtcttt caacttcgtt 187140 ctttttcaaa attgttttga ctatttggga tctcatgagatttcatatga attttataat 187200 gggtttttac gtctttgcca aaaaggacat agggattttgatggagattg cattgaatct 187260 ttaaattact ttgggtagta ttatcatctt aacaatgttatcttttccag tccatgaaca 187320 caggatgtgt ccccatttat ttaagccttc tttaatttcagcaacgtttt taagtttttg 187380 gtgcataagc cttacacctc cttggttaaa tttgttcctaggtatttctt tttgactttt 187440 tttttcattt tttttttttt ttttgagatg gagtctcgctctgtcgccca ggctggagta 187500 cagtggctcg atctcggctc acggcaagct ccgcctccagggttcatgcc attctcctgc 187560 ctcagcctcc cgagtagctg ggactacagg cacccgccagcacacccggc taattttttt 187620 tttttttttt tttttttttt tttagaaaaa acggggtttcactgggttag ccaggatggt 187680 ctcgatttcc tgacctcggg atctgcccac ctccgcctcccaaagggctg ggattacagg 187740 cgtgagccac cgcgcctggc ctttgacatt atttaaaagggaatggtccc cttaattttt 187800 ttttcatatg gtttatggct agtatataaa agtaaaacttttatttctga tcttgtaacc 187860 tgcaattttg ttgaatttgt ctattacctc taatatattcttttgtagct cctttagggt 187920 tttctatgat aggatcatgt cacctgcaaa tagagatagttttacttctt cttttccaat 187980 ttatatgcct tttatttatc attcttgcct aattactcagactagagctt ccagtaaagt 188040 gttgaatagc agtggtagaa gtgggcacct tgtctgttcctggtcatagg gggaagcttt 188100 tagtctttca ccgctgaata taatgttagc tgtgggattttcattaatgt cctttatcat 188160 attgaggaag ttcctctcta gtccttgttg gccaggtgttttcaatcatg aaggggtgtt 188220 gtgtttgtca aatgcttttt gtatgacaac tgaaatgatcatatgggttt tttttcttca 188280 ttttatttta tttttttaat agagataagg tcttgctctgttgcccaggc tggaatgcag 188340 tgccatgatc ttagctcact gtatttttga actctcagacccaagggatc ctcctgcctc 188400 agcctcctga gtagctagga ccacggtcat gtgtcaccacacctggctaa ttttctttta 188460 attttctgta gagctggggt cttgctatgt tgcccagactggtttcaaac tcctggtctt 188520 aagccaccct cccttggcct tccaaagtgc tagggttacaggtgtgagcc actatggccc 188580 tattttgatt ctttatttct tttaaaatag attttgtcagcctgtgtttt ctaggaatgt 188640 ctctatttca tctaagttat ctaatttgtc actgtaggccgggtgtggtg gctcacccct 188700 gtaatcccaa cactttggga ggccaaggca ggtggatcacttgaggccag gagttcgaga 188760 tcagcctggt caacatggca aaacctcatc tctacaaaaaaaaaaaaaaa aaatagccag 188820 gcatggtggt gcactcctat agtcccagct acatcagaggctgaggcaga gaatcacttg 188880 aacccgggag gcacaggtta tagtgagctg agattgcaccactacactgg agtgtagtgc 188940 aatgcactcc tgggtgtttt tgagactctg tctcaaaaaaaaaaaaaaaa aactttccta 189000 tttgttttct atgtcatata tttccttttt attcctcagctaatacatta ttgctttctt 189060 ttgtgtttag ttttttcccc tagtatgcta ttttgattccttcttctttt ccttttctgt 189120 atatttttta gtcattttct tattagttaa cgtggaattacacttaacat tctaaattga 189180 aaacaatcta ggttgaattg ataccagttt actttgagtagcttaaaaac ctgcttctgt 189240 ttacatttat ctaaatagtt acctttactg atactcctttttcttcttca tatggctttg 189300 agttaagaca gtttgattat ggcatgtgat tatgatgtgtcttcatgtgg agcttttgga 189360 gtttttcata gttggcattc agcttcttgg atgtgtagatttatatcttt taccagattt 189420 ggaaaggttt ggccattatt tcttcaaata gtctttctgcccctttctct ccttctggaa 189480 ctcccataat gtgtatgttg gtctggttga tgccacagtttccttagtct ctgttcactt 189540 tttcatcctt tttctttctg ttcctcacac ttgataatttcatttgtcct atcttcaagt 189600 tcactggttt tttttcctct gcctgttcag atctgctgttgaagccttct agtgaaattt 189660 taacttcagc tattgtactt ttcagtgcta gaatttctatttggttctat tttcgatctc 189720 tttcatgata ttttttcttt gttaagtcat catcttcctggtacttcatc catagtgttc 189780 atttgctttt tgaacacatt taagagagtt taagattttgttgagtaaat ctaatatctt 189840 ggtttcctca gaggtggttt ctatattatt ttcttttttttttttttttt ttttgagatg 189900 gagtctcgct ctgttgccca ggctggagtg cagtggcacgatcttagctc actgcaagct 189960 ccgcctcctg ggttcacgcc attctcctgc ctcagcctcccaagtagctg ggactacagg 190020 tgcccgccac cacacctggc ttattttttg tattttttagtagagacggg gtttcaccat 190080 gttagccagg atggtctcga tctcctgacc ttgtgatccacctgcctcag cctcccaaag 190140 tgctgggatt acaggcgtga gccaccacgc ctggtggtttctaacatttt tctatattcc 190200 ctgtgaatgg cccatacttt cttgcttctt tacatgctttgtaatttttt gttgtaaact 190260 ggacattgtt ttaaagcagc tgtatccttc taagagggacattttggata ttacagtgtt 190320 gtaactctgg aaatcagatt cttcctcttt cccccagggcttgcggttgt tgcttgttgt 190380 gggctgcagt cattcattca tttagtgact tttccaaactaattttgcaa aatctgtatt 190440 ccttgtcatg tgtattcact gaaatctcca tttcattatctcagccagtg acagagattt 190500 ccttaaatgc caaattttta tagcctcttt cttcattaaacactccttgc ttgttattag 190560 ttttttatta gattccagag ttctcagaaa gttgctctggtcgtttttgc caactcaatg 190620 gttgcttcag tggaggtatg atttctttga gttccctactctgccatttt tgacaacatc 190680 tgctggactc ttttttatgg aacatgattt ctaaattaacagttatttaa tgaaaacaaa 190740 tttttaaaca aatatatact gatgtttatt ccatagttatctttattctt attagttctg 190800 aagtgattaa atggcattat ataaagccta agcatcttaatttagaagca gaaataatta 190860 tttttataac tcttccaata ttttccccac ttgtatctcagaatttaaac aggtgagaat 190920 ttgtcatttt agaactactg tgaaatagtg tcatttataaaaagagtagg tccatcagaa 190980 acagcaagag aaaatgcagc acacttactc tgttctctctttctgatata attttaaagc 191040 aggttttctg agctttagca gttgacagat cattctttttgtggggatta tcctaaactt 191100 aaggatgttt agtggcatcc tatgactgta ctcattaggtgctagtaaca ccttttttcc 191160 cattaatgac aaccagaata tgtacccaga cattgctgaacaccttccag caggcaaaag 191220 tgtctctgat tgagatcact cttttaacat aaaatgtatgccaagaccat aattaacttc 191280 tgtatttccc aagggcggca actgtttagt gttttcacctcattttacaa atattagaaa 191340 gctggccatg tctaatcaga cagttctgtt ggccattaaggaaagtggaa tggtggaaaa 191400 gccctaatat ccctcttgtg ttactgttag tactacagcttcatttggct aaatgtgttg 191460 tttcgttccc taaatattaa tttactgtgt ctcagatggcacaagaatag gtatttcttg 191520 cctgaaatac ccaggtgatt gaaaagaaaa ttgattccttttccctaaaa gaacatgagg 191580 ttattgtacc taaaataatt ttttagtatt ttgatgatcataattcaggg atgttttatt 191640 atactacagt atctttaagc taaaaaataa attttcttaaaattttattt tcttagaatt 191700 tatttttaga tgtacccctt gtaacattca agccccaaaaatagatgtta gtagtattag 191760 ttttatatgg aattttctcc tatattatga atatttaaacgttgtcacat acggttttat 191820 attattaagt atctggaaga cttggtccac atcaaatttataattcttga agatgtcatg 191880 tccatttttt atattactgc ccttttttat taacaatgatatcataaata cctcactgca 191940 ttagtcttaa tcacttgatt ctagaggtag ctagtctgaaaatacaattc caaatccatt 192000 gctcctgtgg cttaatctta tggagatatt ggggtgggagaaaggagtat tgatcatctg 192060 gattctgagt gagtaaaaat atttcagtac caaattatgatgtccaggaa gctaagaagt 192120 aaccttaatt tctggtttac tatacagcag attaaccaaaaaatactata tttaaagcta 192180 aacaattttt tcgagtacag tttgttatgc tttagtttctaaaaagtctt ttctgtagtc 192240 ccgaatgctt ttgtgtattt tataccttgg tacattctaaaacaaacctt agtaatactt 192300 gatttgtaat agtcaagtta aacccaaggt atagataattataggtgact ttgaagataa 192360 ttgtttcata gaattacata atgatcaatt atcagttgatttttagtcct ggtaaagctt 192420 gatgtaatag gattcaagcc aggtggaatt tgtggtttgcaaggttttat aagagagaaa 192480 ccatcagtag ttggtatgga atgtgcttca ctttacttgtattttaagtt tgaagtgaac 192540 aactaatttt gttttgtctt tagctgtagc accagttgtacccgatttaa gtagtgacat 192600 tgatactagt aattttgatg acttggaaga agataaaggagaggaagaaa cattccctat 192660 tcttaaagct ttcgttggca atcaactacc ttttgtaggatttacatatt atagcaatcg 192720 taggtaagta tgctaaacag tgattatagg agtaagtgctgattttgttc ttagaaatat 192780 ctaaaaatca tttttaaagt agatttaatg ctatataaagatacacctta ttatgcaatt 192840 tttcattgat gcatgatatt tccagtattt catatttaatttctcttgga ctcatccatg 192900 tttaggacat tgtttttcaa aatttttttg ctaaatgatatactatatat cctcaagtta 192960 caacaattaa taggtcaaaa aatgccataa atactgatattttgatctgt agacttatgg 193020 tttataatac attttgaaac aacatgtaga ctttttttgttttgttttgt tttgtttttt 193080 tccacgatgg agtcttgctc tgtcacccag gctggagtgcagtggcacaa tctttgctca 193140 ctgcaacctc cacctcctgg gttcaagcag ttctcctgcctcagcctccc gagtagctgg 193200 gattacaggt acctgccacc acacccagct aatttttgtatttttagtag agatgtggtt 193260 tcaccatgtt ggccaggctg gtcttgaact cctgacctcatgatctgcct gcctcggcct 193320 cccaaagtgc tgggattaca ggcgtgaccc accgtgcctggccagcatgt agacttttct 193380 agcattatca tcaccatatt gatgctgtat caatttttagctcttcaaaa nnnnnnnnnn 193440 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 193500 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gctcacgcctgtaatcccag cactttggga 193560 ggccgaggcg ggcggatcac gaggtcagga gatcgagaccatcctggcta acacggtgaa 193620 accccatctc tactaaaaat acaaaaaatt agccgggcgtggtagcgggc gcctgtagtc 193680 ccagctactc gggaggctga ggcaggagaa tggcgtgaacccgggaggcg gagcttgcag 193740 tgagccgaga tcgcgccact gcactccagc ctgggcgacagagcgagact ccgtctcaaa 193800 aaaaaaaaaa aaaaaaaaaa aaaaaattaa gtgattcctccccaaatttt accatgtgtt 193860 ataaaatgta gtattacctc cactgtgacc ttggacaaataactttggcc ttttatgcct 193920 ccatttcctc aaaaataggg atgatgataa taacaataatactgcttgcc tacttacctc 193980 ataggattgt tataaggttt aagtaaatta aaacacagtacagcacttag aagagggctt 194040 tgcatggtgt aagcactcag tgttaactat tattaccattagtatgagtt catgttagct 194100 ttactctaat gtacttgaga tttaagttaa acatttaacattattttagt gtgctgagca 194160 ttcattcatt ccacagatat ctttgataac ttattatttgtcaggtatat actgagcacc 194220 aaattgcatg gagaaggatg aataagatac agtgccttctcttgagatgc tcacatcttg 194280 gtgagaaatg cataaattgt gcaatgtagg ttagtaagaactacatagta gaaatctcgg 194340 ccaggcacgg tggctcacgc ctgtaatcgc agcagtttgggaagccaagg tgggcaggtc 194400 acttgaggtc aagagttcgt gactagcctg gccaatgtggtgaaaccccg tctctattaa 194460 aaatacaaaa attagccagg tgtcgtggcg tatgcctgtaatcccagcta cttgggagac 194520 tgaggcagga gaatcgcttc aacctggggg gcggaggttacagtgagcct agatcacgcc 194580 actgcactcc agcctgggca atggagcgag actctgtctcaaaaaaaaaa aaaaaaagaa 194640 agaaaaagaa atctaaacaa agtgctatac cagtataatgcattgaacta tatgataaca 194700 cttactgggt gggttaaatc tacttttaat tacctcaaaaatatgtactt ctaatcaaat 194760 tatactcctt tggaatggta tcagataact gattggcttggagtaaatta ttacatttga 194820 ggtcattata actaatctta ttttaaagtg aagggtcttgtcattttagt gtaagagcca 194880 ttcaagttaa tcttagaaaa caaaaagaca atttagtccggctttttgaa acttcttcag 194940 taatccactg taagatagta cttaatactt tttgttgcaacatcaatgag atagctggca 195000 cttatctttt tttcctccct aacactctgt gagtataatattataactgt attcaattaa 195060 catacatata cagaagagct tcagggtggt ttttgctgaagcattctttt tttttttttt 195120 tttttttttt ttcgagacaa agttttgctc ttgttgcccaggctggagtg caatggcacg 195180 atctcggttc actgcaacct ccgtttcctg ggttcaagcgattctcctgc ctcagcctcc 195240 cgagtagctg ggattacagg catgtgccac catgcccggctaattttgtt tttttagtaa 195300 agacagggtt tctccatgtt ggtcaggctg gtctcgaactcccgacctca ggggatccgc 195360 ccaccttggc ctcccaaagt gctgggatta caggtgtcacccactgtgcc cagcctgctg 195420 aagtgttaac tttttgtatt tttatcaatt attctttgacttggtttctg aaacactcaa 195480 gttgttgata taaacaaccg ccaatgtttt tgtattttctgtagtagagt ccagcagtta 195540 ataactttat ttaggtttct ctgtataatg gtaaccttcagttaaataaa catttattat 195600 actatttgtc ctacattatc atgcacttga ttatgttctctagcttttct tcgtattcat 195660 gttttatggc ccaaactaat ttttaagttt cttaaaagtagaaattatac tgtgttctta 195720 actgtcacag cctattgaca aataaatata gtattttttaagtaatcact tactacctga 195780 acaggcaaag gtacggttgc tgttcataaa gaaagaaccaacaaaatata tatatatttt 195840 tgaaacaaga gtctcactct gtcgctgagg ctggagtgcggtggtgtgat cttggctcac 195900 tgcaatctct gcctcctggg ttcaagtgat tcttgtgcctcagcctctga gtagctggga 195960 ttacaggtgc gtaccaccat gcctggctaa tctttgtatttttattagag atggggtttt 196020 gccatgttgg ccaggctgtt ggccaggctg gtctcaaacccctggcctca agtgatccac 196080 ccaccttggc ctcccaaagt aatcccagcg gattacaggtgtgagccact gcacctggcc 196140 ctggcaaaat attgattagc tttattgtaa tatgtaagcataattttgaa tatacagata 196200 tgaaatagaa aacctctgaa attcttatcc ctgaaatccagatatacttt taataccaag 196260 ggcagaaatc attgttgctt aattatagct gaattaaacatgatttttaa aatagcctta 196320 agctatattc ctaatatagt aactcccgtt ttgtaaaaagttacctaaat gaccagattc 196380 tatgtaaaag caccaatata atatgcaaat gtaccttgatagagaaaaga cagaaggtgg 196440 aagaattaat ggatgtatac aagtgatgtc cttacatttcctttgctgca ttcaagctta 196500 tattactaat tttgcagaag actaaaatgt cagtctcagtcacatagcat gttaagttta 196560 gaaaatatct tagagatgaa acagtctcat ctaatttagatgccactggc attgctatat 196620 gccttattta tattacataa gcagaatctc acattgaatttctgagatgc ttagtagtca 196680 atggaattaa attgcaccac acctagaatt tgtcatttgttatatgggac ttttcctcta 196740 taaagctgct acacagcccc tattattgtc ttaaagttatttgaatctcc atttgatctg 196800 aataattttt caaggaaaac ctaggaagtg gcatatttctagctgtttct tttatagtac 196860 cattgtactg tgtatgttta ttgatactta atggatacttgattatcttc tgttattcac 196920 taatttttaa aatttactta aactgttctt ttggaacattattaatatct ggtgtgttaa 196980 cacatgcctc caatagctat gaagtatact gtaactatttgatctccttt tgcaatagct 197040 ctactggtta atcagggtgc tgactgtagt ggtggtgatggtagaagtag tgattatggt 197100 agtcgtagca tggtggcagt aggagcaaca ctggcagtactagtacctta cctttatata 197160 atatttggta gcttaaaaaa ttgtttacat atatgatctatttagattgt ttgagaatct 197220 tgtgagacat ctaaaaaaga cctctaaatt agttcactaatagctttatt tatatacaat 197280 tctaacttta cactgaattt aaagaactct gcaaggctgggagtcccaaa agattagcag 197340 catcacctaa actttggttc tgttatttca cccacataggcaaaaacttt tttgacccga 197400 agaggcaata ttatgctttg ctgaaggata gttgggtgcaggagaggtgg ttatgcatat 197460 gtctgcagaa gtcagcagtt tcttaccccc aattcttatatacaaaaagc tctgaaaact 197520 gaatgttttt aagttatttg tttggtggaa aaacctgtcataacctgctc tcatgaccag 197580 aaagcctaat ctgaactaac ttgagactat ggtttttatttatcctactt ggtatgaata 197640 ttcttatgtt ttgctgcagg aatattaagt catatgtttacaggctgcta ctatttttag 197700 acctaactgg gtgttttatt taatatatgt atcatttctgaattatgttt ataaaaatta 197760 tgaacctata cttaattttt aaaaacaaag ttactataactgaacataaa ataggcgtgt 197820 agtatttatt attggttgtc atgggatatt acagagttttttgttttctt ttctttttaa 197880 acttttcttt ccctcagata cttatcttca gcaaatcctaatgataacag aactagctcc 197940 aatgcagata aaagcttggt aggtattttc ttagtattaagaaaattgtt tttaaaattg 198000 ttttgtggat aatatatttg tgggtatgat agtaaatttatatattgttt gtgaagctgt 198060 ggttagctga acttagattt ttatggcact ttcatctcccatctcctttt cagtctgatt 198120 ttttgctcaa atatcaaatg gaataatata tttggaggcagttagtaaac taaattgtta 198180 cacagattga cagtggtcat cttttttaat cttcggaatcaggaactatg atatatcaat 198240 gtatcattaa gggtcttgac aggcaaacag attacatgttcaaaatattt aaacagagag 198300 ttttgtgtgt atggcaaata tacgtaacat gaaatttaccgttttaatca tttttaagtg 198360 tgcagttcgg tggcattaag caccttcaca tagttgtgcagccatcactg ccatccatct 198420 ctagaacctt ttcatcatcc caaactaaaa atctataccaattaaacagt aactcctcat 198480 ttcaccctcc ccccagctcc tggtaaccac tgttctactttctgtctcaa tgaatttgcc 198540 tattccaggt acctcatatg aatggaatca tacaatatttatccatatgt gactggctta 198600 tttcatgtag cataatgtct tcaaggttca tccatgttgtagtatatatt aaaatttcct 198660 tcgtgtttaa ggctgagtaa tattccattg catacatatatatacacaca cacacacata 198720 tatacacaca caccatattt ttgcatttat taaatcaagagtttaatgaa gggatgatta 198780 acatgctgtg aacatcagta gagggaaaca ataaagggtactaaagcacc catgtatgaa 198840 caagagtggg aagccattgc cctatactga aggagcaaagagagaaaatc ttatcgtagc 198900 tcagagaaag cctgacatca tagaagagga atataaaacgtagagcattg tgaaaaccac 198960 agtaaagcac aaagggtgta gggaaatgaa tatttctttcttttaccttc agatttctgc 199020 tggtgtctcc tgtgggttga aaccaacctt aaaatcatagggctacagag taatacaggc 199080 aatgcaatct gtagatttta aggcaaagaa gagcagaggatgaattttca aggttagaag 199140 ggaatggggg aaatggagaa taaccagtat agttagcatcatcttaattt gtccactgag 199200 aagtatatta ctccagaaaa tacattttag ttgagttctttctgttgttt atatagctca 199260 tgttctgaca cagaggtaaa acaaaatctt acttgcttacatttcagaag gtccttttgg 199320 gttaacagga attttctgaa aaatatccag agtaattatctgtccattcc tagagcattc 199380 tttgaacttt ttgtctacta tagcctacat ttttttgtgggggggcacag agacattgca 199440 agcctacata tttttaacag aggagaggaa gaggtagcagccatttttaa aaagctgata 199500 atgattcttt tctttctcct tgatatattg actcttaaatataagagaac atgcggtgaa 199560 aagggaaaat gagaaaaagg tgaaggaact atatgagtactcatttggtg ttaagttcat 199620 ctttctctta aattttaatc ccagtagagc tgtttggcaagttatgtatt gctaacaagg 199680 gctcaaaaat ctttgaccta cgtaataata aggagttgctgtaagtcagc tctcactgtg 199740 ttacttgact gtagagttag aagtgccttc ggccttcaaaaatattcaaa ggattcatgt 199800 tttctttggg ctgattttgt tcagtggtga ttaccataagtttatcagtg gttgctacct 199860 ttatcttttg ctctaataat atcagggggg atatactaattatttcaacc tagaactaag 199920 tacaaaatca actaatttaa ttttccttga aaacctagcagagatgctat tgaacaactt 199980 tgcaattgcc aaatacagtg gatgattttc agcccatatcttatttttct tctttgctgt 200040 atttgacact gactgcttcc tacttctttg gaacttcaaacatttctaaa tcttctatgt 200100 tcctattgtt tccctctttt ttacctccca tatttttgaattttatttat ttttcaatcc 200160 tcttttcagg atcttcctcc tctgctcact ttcaattattaacatttccc aaagttctga 200220 tcttggctct ctgctctttc tctgtaggta atctcattcatgtccagctt gcttgtctga 200280 gctccagtcc tgaatctttc tctctcttct gtatacttctcaggcatatc aaactaggcc 200340 agagctaaac tcactgtttt cctgtcccca ttccctccctagcagtgtgc tggctgtctt 200400 tcataatggt attatatcta ttctaacttc cagtcggtttggtcatacat tgtgctttcc 200460 aattgatacc tttatccatt acttcctctt cattcctgtagcctctgcct tttatcagat 200520 ccatattctc ttgtctgaac tgctttgata gctttttaactggttgctct tcttagaact 200580 tcactgccct ctaagctgtt cattaacctt attattgtagtgatgtttct gaggtgccag 200640 tattatgtca tttctcattt aaaatgctta tggggtaaagtccccaactc cagtttgtgc 200700 atacaaggat cattaccttc ttaggcttaa tgtgagttgtttttcctatt acattctgtg 200760 ctctgctata gtacttgatt tctttccatt tcccaaatatatcatatttt cttctgcctt 200820 tctacttttg ggctcattgt tcattctagc taaaatgcctttcttgtgcc accttctttg 200880 ctttatcaga ttatcagttc ttcttatctt ttaggcctcaactcaaggat tatatattct 200940 tggcagagtt agaagtgacc ttttctctcc tttggaacactctgtagata atttttttat 201000 tatttaccac agtctattgt aatattttgt gtatatgtctttgggacatt gtagcttctt 201060 ttttctcttc caatttaact ttgcctcact atgtaactatttgcttcatg agaacaggaa 201120 ccaaatcttt ttaatttttg tatttctaat ggcctaacgtttagtaaatg ttaagaaagt 201180 gcttgttaaa ttaataataa aaaaattgag atagaaagaggtaagattaa attaaattat 201240 tttctaaaaa ggtttaaaat attaacatag acttgatatttttaaatgtc taaatattct 201300 tagtagaatg agtttttgtt tttgttttaa attaaagcaggaaagtttgc aaaaaacaat 201360 ctataagctg gaagaacagc tgcataatga aatgcagttaaaagatgaaa tggagcagaa 201420 gtgcaggtga gaatatactt taagtttttc atattaaaagttttatatac caaatactta 201480 cttgaaagct tataccctgt aaacatctga aagtaaatagaaaatgagga tgtaaagatt 201540 actactgata atacattcat gatgtgtctc ggctagaattgagaagttgt atttatgttg 201600 gcttagttat agatgtattt tcctcagaat atttaaagtacatttgttcc tggcagtctg 201660 tattcttttt ttttttttta attcatgaaa ataatatattgtgtactttg taatatgttc 201720 caactgtgtt ttcattacag tcatcagaaa gtaaagcagtattctttttt ttttttttta 201780 agatggagtc tcgctctgtc gtccaggctg gagtgcagtggcacgatctt ggctcactgc 201840 aagctccgcc tcccggattc acgccattct cctgcctcagcctcccgagt agctgggcct 201900 acaggcgccc gccaccacac ctggctaatt tttttttttgtatttttagt agagacgggg 201960 tttcacagtg ttagccagga tggtctcgat ctcctgacctcgtgatctgc ccgcctcggc 202020 ctccaaaagt gctgggatta caggtgtgag ccaccgcacccggccaacag tctgtattct 202080 taaaaaactg taatgctaat tcatttagtt gcctgacttactgctttttt ctctttacat 202140 aattagctaa ctaaaagggc aaattaggct gcacatggtggatcacgtga gggcaggagt 202200 tcaagaccag ccaggccaac atggcgaaac cgcatctctactgaaaatac aaaaaattag 202260 ctgggcacga aggtgtgccc ctgtagtccc tgctgcttgggaggctgagg catgagaatt 202320 gcttgaaccc gggagggaga ggttgcagga agtcgagatcacaccgttgc actccagcct 202380 gggcgacaga gcaagactct gtctcaaaaa gaaaaataaataaaggggaa attagtttac 202440 attaaatcag gtgctttttg aaatgtaaaa tgttgaagattaaaatatac taaatttttg 202500 atgtggccat tctaatttcc actgtggttc accagagcagtaactcagag tatctttttc 202560 tgtgggtcta atttcagcat gttattgtca ttagactcacagaaaaaata tctttgagtt 202620 atgctcacgt ttattttaaa ttacctagca taatactgaattacttatca attataagca 202680 ataatttttt ataattattt atgtttttag tgaatagtattgaataaatg aaatgcaaag 202740 gtaacacgaa aagtattcaa caaataaaat tgggaacactggtaattttg tgacagagca 202800 ggtggggggt ttggtacaaa acagataaaa ttctcacttttaaccattcc tgatagatta 202860 tgtggttaag tgtataatac aaatttcttt taaaaaggcatttgaatata agcaaataat 202920 taactgaccc caggatttgg aaagaatttc taagtataaaatatgtaaat aaggggaaga 202980 actgggtaga tactgttata taaatgttgg gaagtctctacatccaaatt tttttttttt 203040 ttttttgaga cgaaatctca ctcttgtccc ccaggctggagtatgatggc gcgatcgcgg 203100 ctcactgcaa cctctgcctc ccgggttcaa gtgattctcttgcctcagtc ccccgagtag 203160 ctgggattac aggctcctgc caccacgcct ggctagttttggtattttta gtagagacgg 203220 gatttcacca tgttggccag gctggtctcg agctcctgacctcaggtgat ccacccacct 203280 cagcctcccg aagtgttggg attgcaggcg tgagccaccgtgcctggccc aaaaaaattt 203340 ttaaacaaaa ttaaaagaca agtgacaaac tggtagtaatggtgagtagg gatcagtttg 203400 aggaagtaat aagaatggct gaattggcta ataatcatatgaaagtatca cctcaaggaa 203460 ataaaattga gatgaaactt taaaaaatta tatattgaattggtaaatag tttttaaaat 203520 aaaaacctag tgctagggat tgtggagtga tatttacttgcatttcttta ttttatttat 203580 tttttttttg tttttatttt ttttgaggca gagtctcactctgtcaccca ggctggagtg 203640 cagtgccgtg atctcagctc actgcaagct ccacctcccgggttcatggc attctcctgc 203700 ctcagcctcc cgagtagctg ggactacagg cgcccgccaccatgcctggc taattttttg 203760 aattctttag tagagacggg gtttcactgt gttaaccaggatggtctcga tctcctggcc 203820 ttttgatccg cccacctcgg cctcccaaag tgctgggattacaggtgtga gccaccacgc 203880 ccggccactt gcatttcttt aaatcaaatt ctctgcattcaaatcatttt agttacattc 203940 atttattcaa taaaaatttc agttaaagtg tcatataatgtgcttgtcaa caatatagta 204000 ctgaataaat cacgacatgg cccctctcca caaggagcttgtaatttagc aagaaaaaca 204060 agtaagtaat tatcaagaag tgtggtaggc caggtgcggtggctcatgcc tgtaatccca 204120 gcactttggg aggccgaggc aggcggatca caaggtcaggagatcaagac atcctggctc 204180 acaaggtgaa accacatctc tactaaaaat acaaaaaattagctgggcgc ggtggcgggc 204240 gcctgtagtc ccagctactc gggaggctga ggcaggagaatggcatgaac ccgggagacg 204300 gagcttgcag tgagctgaga tcgcgctgct gcactccagcatgggcaaca gagcgagatt 204360 tcatctcana aaaaaaaaaa aaaaaaaaaa aggaagtgtggtatgtgtta attggggaga 204420 gaataaaatt atacaggaat atcaggaata catcaggagatgcctgtact gttttgtcag 204480 agatcagaga aagctaaacc agaagagtca atggaaattattaggagaag agtggaagga 204540 agtgttaggg tccaggaggt gagtatctgt gaaggccgtgaggtaagaga ccttgactgg 204600 ccagtaagat gtgagggaag gaaaagcctg agatgagaatggggaggatg gcaaggtcta 204660 gattggggag aatttttttt tctctttttt tttgcgggggggctaagaaa tttggaattt 204720 aagtgtaatg gaaaaacatc aaaagtcatt aaactaataagtaaaaaata tatattaaat 204780 ttgctgtttt caaaagaagt tcctgcttaa ttagcagcacttggcacttt attcagctaa 204840 atgggagtga gggaggatga gtcaaggatg attcctaggtgttgtcattt acttagggac 204900 tctagaagag aagcaaattg gtattggggt gggagtgtggaacagaggca gtaagttcta 204960 ttttggagat gctgaatgag gtatttaatg agacatccacgtgaaaatgt tgcatatata 205020 ggttttgaag ctcagaatta cattttgaac caaagaaagcaaatggtaat gaagataata 205080 cactttgagt gttgccaaat ggtgccttta ctgatttgatatatactacc tttctctttg 205140 tttactttat cctgtattaa aggataaatg gctaatttttgagtagtggc aagtattgca 205200 tgacggagtt tagggatcct taacctgaaa tccatagacctcccccaaag agtccatgga 205260 tgaaatttga agtccttaaa cgtggatgac aaaaggtgaacgttttctct aacttataac 205320 ttaattttag cacttgtttc aattctgagc ataggcagcaattcagttat tagcagtagt 205380 tgttaatttt tattaccaat agaaattata gatgttttcttattacagtt gttacagcta 205440 tctctaaata tcatttatgc tcatcaatac tttgaaatcactgccactag atgttgtgat 205500 ttaatatatt aataaggaag catatatact actgaattacacatattttt ttaaagttta 205560 ttactgtgtt tcaatataat tggcttcttt tttttttttgagatggagtc tcactgtatc 205620 acccaggctg gagtacagta gcatgatctc ggctcactgcaacctccacc tcccaggttc 205680 aagtgattct cctgccttgg cctcccaaat agctgggattacaggcgccc gccaccatgc 205740 cttgctaatt tttttttttt ttaattttta gtagagacggggtttcacca tgttggccaa 205800 gctggtctcg aactcctgac ctcagatgat ccacccgcctcagcctccca aagtgctggg 205860 attacaggtg tgagccactg tacccagcct aattgtctcttttaatcata tataattttt 205920 gtgcttcaaa aaatattttg aaaaaaggcc ataggcttcacttgactgcc aaagagatcc 205980 atggcaccaa gaaaggatac aaaactctag ttaagtggaaaagtaaggaa tgttagtgtt 206040 taacactggg caataagaaa tacgtatatt tctaatctgtattgcttttg tttttacaga 206100 acctcaaaca taaaactaga caagataatg aaagaattggatgaagaggt actttttgtt 206160 ttctaatcaa gtttcctttt tgagattttt ccctctttcaaattgtttcg tttcctgaga 206220 aaattgtatt tgaattctgt agtttgaaac acaaaacagagcagagaatt gtgatgaaag 206280 cctcaccttg gaactgcagt ttcaagtggt acagattagattataattta gtttcctttg 206340 tacaattgtt ttctacagcc gtattaattt tagtacgtaaatttcagttc agtggttctc 206400 gatggttgaa atgtgagaaa ggagccagag aagcaagcgatcagaaattg aatctcaaac 206460 tatgtcagaa agagacagac aaataaggaa aagagagaaatacagtttaa aatattttta 206520 aaagattaac actgacataa tctcatgtaa tcaaatggaaaatatattga ttttagaatg 206580 ttcatctttt ccatggttac ctgtcaaaat tttttattatcagagaaaca ataacaagca 206640 ttcataattc agatgtttgt ttgcaaatat gtaatacatttccattcctc ttagtcaact 206700 tctaagtata tttctactgt gtctatgagt cccatgaaaagaaatacact ctctgtagtc 206760 ttctcctaat acattactta ctttgtcctt ttttaggtttaatctctgat agtttttatg 206820 ctgtgttaat tttttcttcc cttatagaat ggtatctgtcaatggcttgc ttgctttcat 206880 gggtagcttt ggatatctat gaagatttag agagcatttactaacttgcc tttgtactta 206940 accttagcag tatatatttg actctgagtg tttaaggagtccttttttgc atatcttcca 207000 cttatattta acttttaaaa cacagataaa attcagatgtgctagattta agttattcat 207060 ttttccaaat tagaagtaga gagggaaatt tgaggtatgaaattaaaata gtctcacttc 207120 tagaaagatg gagtaagagt accttttctg tattcctcccagtaagtaca gctaaaaacc 207180 ctaaacatta aacataaaac aaacatgaaa agattttgaaaggtggaaag aaggcagacc 207240 aacctgggtt acttgcgtcc tgagaaacaa cacatgaatgagtcttcagg gttttctttt 207300 tgctgcatgt atcctagacg gggtgccgga gaagccagcaacctcgaaat atcaacagtt 207360 gcagacaaag ctccaaaaaa agctaatttc cttagcctaaagaccaaaaa aggagcaggc 207420 tagcaagtca gaaaacttac actatcactg ctcttttcagccaaacacca cagaaaaacg 207480 gtagcctcat tctttttttt tttttttttt tttgagacagagtcttgctc tttttcccag 207540 gctggagtgc agtggcgcga tctctgctca ctgcaagctccgcctcccgg gttcacgcta 207600 ttctcctgcc tcagcctccc gagtagctgg gaccacaggcgcccgccacc atgcctggct 207660 aattttttat atattttttt tagtagagat ggggtttcactgtattagcc aggattgtct 207720 caatctcctg accttgatcc acccgccttg gcctcccaaagtgctgggat tacaggcctg 207780 agccaccgcg cccagccaac tgtagcccca ttcttaacagcaaaggctga gtgggaagtc 207840 tagacttcca tctccactat gctgtagcac accccaacttccaattagaa tagtgtcaga 207900 gaaggtccag taaaggaatt ttcatctcct gtgggcagtaacaaaccttc tttctctccc 207960 cagacagcgt agcagtgacc atgtggggaa tctacacttacacttccagc tggcagtaag 208020 ggggtgcccc tcccactgcc ttctgggttg gtatcaaagagacatggtag ggagtcagaa 208080 ctttccactg aaaataacaa gctcccctcc acaatgcagtgtcatctgtg ggggccacat 208140 aaggagcagc catggtgcac tcaatggagg cctggtagagtgccagaact cctgcttcca 208200 cccagcagtg atgaggatct gctcccctcc ctacctgaggtgtcagtaga ggctgagtgg 208260 aaaacctgga cttctgcaaa ctccacctgg cagaaaagaggcagcacccc cttagtttca 208320 ccagaacagt gtcagaggag accttctaaa agagattttaaataagatcc agaatgttgt 208380 aagatgatgt ccaggtttga atagcaaact gctcatcataatcagaacta ggaaaatttt 208440 aactggaatt agaaacaata ataaactaat accaatgctgagatgacaca gatgttagag 208500 ttatctgaca agtatttaaa ggaatcacca taaatatgtttcagtgagca actgtaaaca 208560 tacaaacaag caaaaaaata gtctcagaaa gacatagacaatataaaata gagccaacca 208620 aaaattacaa taactgaaat aaaagtctca atagatggtgtcagcagcag agtgaacaat 208680 gcagaggagc gaatcagaga ccttcaacaa agaaaaatagaaattatgca gtctgaacaa 208740 cagagagaaa atgatcagaa aaaaagtgac agagcctctgggacctgtag aactataaca 208800 ataaaattct aacatttgtg tcactcgaat tactaaaggagaggagaaag ggcgtgggaa 208860 gaaatgacga cagaaaattc aacaaatttg acaaaagacctgaactgaca aaagacatga 208920 actaccaaga agcttagcaa accacaaaaa gtgtaaacccaaagatatcc acaccaagac 208980 acaatagtca gatttctgaa aattaatgac aaaaaagaaatcttaaatac aggacacacc 209040 ttacctagaa aggaaacaac agtgtaagtg acagctgatttcttatcaga aaccatggag 209100 gccaggagga agtggcatga ctttttcagg tgctaaaaaaacaaaaaaac aaacctatca 209160 acctagaatt ctgtgttcaa ttaaaacatc cttctcaaatgaaaaagaaa atgacaacat 209220 tctcagatga ggaaaaacta agataatgtg tcaccagcacaccttcccta aaggaattgc 209280 aaaagaaaat tctctaaaca gaaaggaaat gataaaagaaggaatcttgg ccaggtaggg 209340 tggttcacac ctgtaatccc aacactttgg gaggctgaggcgagcagatc acttgatgcc 209400 agaagtttaa gaccagcctg gccaacatgg caaaaccccatctttactaa aaatacaaaa 209460 aaaattagcc aagtgtggta gcgtgtactt gtaatcctagctactcagga ggctgaggct 209520 gaggctagag aattgcttga acctgggagg tggaggttggagtgagccaa gcgcaccact 209580 gcactccggc ctgggtgaca cagcaagact ctgtctccaaaagaaaaagg aggaattttg 209640 aattaccagg aataaagaaa cagtaaaaga tgggtaaatacatttttttc tcctctttat 209700 tttttatagt aggcagttga agcagaaatt atattgtctaatatgattct ctgtgtatga 209760 gaaaaaatat tttagacatt ataaacagag agtaatgggtcataaagaaa ggtaaagttt 209820 ttaggtcaag tgtggtggct catgtctgta atcccagcactttgggaggc caaggccagc 209880 ggattgcttg agcccaggag ttcaagacca gcctgagcaacagggtgaaa ccctgtctct 209940 acaaaaaata caaaaattag ccaggtgtgg tagtgcacgcctgtagtccc agctactagg 210000 gaggcagagg tgggaggatc gcttgagtcc aggaagttgaggctgcagtg agccataagc 210060 ataccactgt actccagcct gggtgacaga gtgagaccctttctcaaaaa taaataagat 210120 aaaaattaaa agaagaaagg gaagatgttt atactttactcaaactggta agatgccaat 210180 accagtggag tgtactatat attaagctat gcatatacaatgtaatatct agggcagcca 210240 ctgaaaaggc cttacaagga gatacattca aaaacaccatagttaaatca aaaggaatgc 210300 taaaataatg ttcaggtaac ccacagaaag acaggaaagaaaatgtagaa ataacagaga 210360 tcaaacaaaa aaacaaaaac ggcagactta accctaacatactaccaata atgacattaa 210420 atggaaatta aatggagtac caatcaaaag aggtggtaggggtagatttt tttaaatccc 210480 ccatttatat atctgtcaga aactcttcaa atataacaatataggcaagt tgaacatcgg 210540 aagatgtgaa gagataacat aacaaatatt aaaaagaaagcagcatattg gcaatgttaa 210600 taccaattaa agtagacttc agagcaaaga aaattaccatgaacatagag gaatattaca 210660 taatgataag agtcaatcca ccaaaaataa ataaaaatcctaactgtaca ccaaacaaca 210720 gagctacaaa gtatgtgaag caaaagttga taagactggaaggttaaata gaccaatcca 210780 taattattgt tggagacttc agcacccgta agaatcaccaaacataggag aactcaacta 210840 cactgtcagc tgtcagaatt tgttcaacat ttacagaaccctctgcttaa cagcacaata 210900 tgccttcttt tcaagtgctt aaggaacaca caccaaggtagaccacatcc caagctataa 210960 aacaaacaca tttgaaagca ctgaaatcat acagtttcctgaccaaaatg tacagatgag 211020 aataacagaa aaatctgcaa acacatggaa agcaaacaataaacttctaa ataatccaga 211080 ggtcaaagaa gaagtctcag tgaaaaaaat acattggaatgttaattatg tctcataaaa 211140 ctgttatggc tctttatctt tacaaaatat ttgaacaatgaagtgaaaat acatcatcaa 211200 aatttgtgag atgctgttaa catggtgatg aaggttaatttatagcacta aatgcttacc 211260 ttagaaaaga ggaaaagtct aaaatcaata atttaaactcctacttctgg aaactagaat 211320 aagaagagtg aaataaaccc aaaacaagca aaaggaaggacaagtaacga taagagcaga 211380 gaacaaggca gttaaaagca gaaaaacaat agagaaaatcagtgaaacaa aaagctgtgt 211440 gaaaagatca acaaaattaa caaacctcta gcaagactgataaaggaaaa agagaggaca 211500 caaattacca gtatgaggaa tgaaataggg catatactatagaccatata ggtatcaaaa 211560 gaatcataag gaaatcctat gaacagttct aaacacaattaagacatgta gatgaaaggg 211620 actaacttct caaaaatcaa actatcacaa ctcatacagtgtgaaatagg taatttcagt 211680 agccctatat aagtgttaag gaaagcgaat ccatactttttaaacttttt agatatatgg 211740 atgaaaataa acctatgaaa agaggttcac ataattagtcatttgggaag tgcaaattaa 211800 aactaccatg aaatattact ccataaccat gaaaaaaagtaagttttaaa attatgtcaa 211860 tagcaaactg gcaaggatat ggagaaacta gatcacttggacattgcaga tggggatata 211920 aaatggtaca gacattctgg aaaaccaatt ggctgtgttcctgaaaaact aaacatgtgc 211980 ctactatgtg acctagcaat tgtattcttg ggcatttattccagaaaaat gaaaatgtat 212040 attctcacaa aaacctgtat actaatgttt ataacagctctatttgtaat agccaaaaac 212100 tgaaaacaat caaaatgtcc tacagtaggt gaatggttgaacaaacttcg gtacatttgt 212160 accatggaat actcagccac aagaaggaat aaactgtagatacatacaat acagagatgg 212220 attttaaggg ctttatgttg agttaaaaaa aaaaaaaacctgtcttggcc aggcgcggtg 212280 actcacgcct gtaatcccag cactttggga ggctgaggcgggcggatcac gaggtcagga 212340 aatcgagacc atcctggcca acatggtgaa accccatctctattaaaaat acaaaaaaaa 212400 aaaaaaaaaa aagcctgtct caaaaagtca tatattgcatggttccattt atataacatt 212460 ctcaaaaaga caaaattata gaaatggaga accattagttgccaggagta tgggtggtgg 212520 gaggacgggg atgggtgtgg ctataactag tgactggaaagaaattgttg tgatgaccag 212580 ttctgtatct tcgttgtggt ggtgcttaca cgaacctacatatgtgataa aatgagagaa 212640 tcatacactc cttataccag tgagaatttt ctgattttgttattgtacta ttgttatata 212700 taatgtctct actgggggaa gctgagtgga agggcacttgggacctctct gtactatctt 212760 tgcaactttc tttgaatgta taattatcta aaaattaactaaaagttaga atgataaact 212820 cttagaatca gaaagacctt gaatgattta tgtccaatttctgcttaaag atgagaaagc 212880 ccagaaacac tgtaaaacag gtttcctaat gtggagtcttaaaaagtgaa gcatcaaaaa 212940 caagatttac tgatagtggt aactcagaaa ggtacatttttaagttgtga aaatttgggt 213000 attttaggct gggcacagta gctcatgcct gttatcccagtactttggga ggctgaggca 213060 ggcggatcac ctgaggtcaa gagttcaaaa ccagcctggccaacatggca aaagccgtct 213120 ctaccaaaaa tagaaaaatt agctggacgc agtggtgcgtccctgtagtc ccagctacag 213180 aagacagatt cccactgtct tcttaaaagg caagctacttagaaagttgt attaggagat 213240 actatttgcg tttgaaacag aacattcatc ttaagtgtttgtcttttttt ttttcttgtt 213300 ttttgttttt aagaacaaaa tactcacttg aatgaatgaccagagtatta atcacagtgg 213360 taacttttat cattataatt ccagggaaat caaagaagaaatctagaatc tacagtgtct 213420 cagattgaga aggagaaaat gttgctacag catagaattaatgagtacca aagaaaagct 213480 gaacaggaaa atgagaagag aagaaatgta gaaaatgaaggtaaattgtc actacttttg 213540 aaaaatgata gaatttctaa cactgtcatg aggttgtctttaatttagtg tccatgatac 213600 atacatgaag ctacaatcct aagacacttt ataagctaaataacccaggt ctcaggttaa 213660 atttataaca tattaaatag taattaaatt aaatattaaattacatatta aagtaaatag 213720 taagtagcat ttaactagag tagtgattgt catcatggtgtgcatactgg aatctcctgg 213780 gaagctttaa agagtactga tgcctaaatc ccagcgattctaatttaatt ggtctagata 213840 tgtggcctga gtatcattat tctttacaag ccccctaggtacttctaaca agcagctgcg 213900 tctaagaact tctaaaccag aatttcttgt gttttaatgcttgtgttaca aatatattta 213960 aaataccttt ctactcccat tgatgcagta atttcacttccataagttat ttcacgtaca 214020 tactcctact gcaaactgat atatacacaa ggctattcattgtagcattg tttaaaatga 214080 caaaaaaaat gcagtcaatg ctgtatatga aacataatgtaatacatata tgaaacataa 214140 aggtaatgaa tgctgtaata aattttagta catctatatggtagaatact atgtggctta 214200 taaaaaataa taaatcatct ccttaaatac ggttataaaacagtctctaa gacagatctt 214260 taagtgacag aaacggattg cttgtgttta aaacaagagtacttgtctat atatgcttaa 214320 attaaatata tattagggag gagagccagt taagggagactcagaagggg caaaaatgac 214380 taaatggaga aaggaggact tattctatac tatctaccatttggtacctt ttaaattttg 214440 tatttttatt aagatattga attatcaagc aattgaactcttgaaaaata aataatatta 214500 aaactagctt tataagtata ctatcagtga tcttataaagggaataaagg agtttgagat 214560 ttacaacagt aagaagaaat ctttttgcac tctgtattagaagataatgt tctacttttt 214620 aatgaattta aaatgcaggt acttccaaat tggaatttggctttgggagt ggacctaatg 214680 tagctgacta ttatggccta agactaaatg tctttttttaaaaaaggatt tatatgtttc 214740 ccctatgaaa atgttgtaat ttctatatat tattctttcattttctagtt tctacattaa 214800 aggatcagtt ggaagactta aagaaagtca gtcagaattcacagcttgct aatgagaagc 214860 tgtcccagtt acaaaagcag gtaagtgttt caaatgaggttcctttttaa aaaaattgta 214920 gataattaaa ttatatcgta ctttggtcag aaacctatttttttgctttc tttttaaata 214980 gctagaagaa gccaatgact tacttaggac agaatcggacacagctgtaa gattgaggaa 215040 gagtcacaca gagatgagca agtcaattag tcagttagagtccctgaaca gagagttgca 215100 agagagaaat cgaattttag agaattctaa gtcacaaacagacaaagatt attaccagct 215160 gcaagctata ttagaagctg aacgaagaga cagaggtcatgattctgaga tgattggaga 215220 ccttcaaggt aatatttttt cttattgtgg taaaatatacgtaacatgaa atttattata 215280 acattttaac catttttata tatacagttc agtgggattaaatgcagttc acaatgtatg 215340 caaccatcat catataagtg gaatcataca gtgtttgtccttttgcgtct ggcttttttc 215400 acttagcaca atgtcttcaa ggtttatcca tgttgtgtgtgtcagatttt ccttcctttt 215460 tagggctcag taatattcca tggtatgtgt ataccacattttgttcatcc actcatctat 215520 caatggaagg aatattttat aatttttatt tttaattgttaaaaatatat aacataaaat 215580 ttaccatctt gaccattttt aagtctaagt taatatatttatgatcatat taccctcata 215640 aagaaaatga ctttggtgaa accagaaatg gttgcattcttttttattgt ttacgttttt 215700 ttgagatagg aacttgctct gttgcacagg ctggagtgcagcggcatgat cacagctcac 215760 tgcagccttg aactcctgag gtcaagcaat tctcccacctcaacctcctg agtagctggg 215820 actacaggtg catgccacca tgcccagcta atttttgcattttttgtaga gacggggttt 215880 caccattttg cccaggctgg tctcaaactc ctgagctcaagcgatccgcc cacctcgacc 215940 tcccaaagtg ctggcattat gggtgtgaga caccacaccaggccagcatt ctttctcttt 216000 tagaataatg cccacatttg tatcacattg taatttcttcaccctcacag gtactcctgc 216060 agggtatgta atgtggatat ttttcagttt tacagacaaattaaccaaaa acttcattta 216120 aaaacttgcc taattcataa aggttttact caaagtgagactaatccagg caaagaggat 216180 tatcactatt aaaacctagg aattcctcat gcatcgttggtagtaataga aagtcagttg 216240 cagaaataaa atcccattac acattaaaag ttagatacttgggaatgttt gaactggaga 216300 tattgacagt ctttgtgaat ctaagaggca gaagattgagtataactaag tgaataaatg 216360 aacagttttt aaatagtaat aaaattcagt ttgttttcaaaaagattcct ggctttacct 216420 actcacatat ttttctattt tctattcctg ttagcagcagcatccatact acctaatcaa 216480 aacaaatatg catagaagtt gccagatgtc tcaggaaaaaattggaggga ggagttcttt 216540 ttaaccttag ttagagatgg gaacatagtt tcaggtcccttggggcttga tgattagcta 216600 taattcagtg gagagaggtc agggtcagtt tttgtgactaaggaaacaga aaatacaatc 216660 tcctgttggt tcttagctga ggagtgttgt gcaatcatatttctcaggga ccaggcttaa 216720 aatagatctg attataacta agaaagtaac cactttatatgtaataggaa agtcacagac 216780 ttccttaaat tgttttccta ggccttttta tacagcttattagctgtttg aaagtctctt 216840 catctccctt ctttatctgt aaaccaagga gataagaataggttacctct ataaatcctt 216900 ccaattctaa aattcttata agtttgtgat ctgtgtttgactgagcttta agctccttgc 216960 ttcagaggca gagatcatcc atgtactatc aacagtggtttttaactgaa tctatttttt 217020 agatatcagt aaaagttaag gaaaatttat agcatgatttattgttagtt tattcttttt 217080 cagagtttaa tttggaactt aaacgtgaat aaaagctcacatacactaaa ataatgcaca 217140 caacttatgt gagtttcaac atacatataa aagtgcattgtgggttttta aaaaaatcac 217200 aatgcttata ttagtctttt ctggtcttta agaactttcaggtctctcaa gtataaattt 217260 agtttgggat ggtgggcttc tggcagagtc ttgattgttattcattaatt gaatgtcctc 217320 catatatgat ctctgttgca tagtcttttt tattttttactttttataac cttttaaagt 217380 ataaaaacca ttcttagctt gacatctgta cagaaacaggctgcagccct gatgtttgcc 217440 agtacctgga ctagtgcaac agtaaggctg caattgattatagggaaagt ttccaaaagt 217500 tcaggctata gaggcttaat gaaagtgatc aaaatgttatggaggattga aattttaact 217560 gaattgataa gctttcatct agtaagttta tctctgattcttcagattga aaggcaaaaa 217620 tcaatttttt taaattaacc atttttaaaa gtctttttctgtttttttta attgtttgct 217680 tggaaccaga agtgcttcag attttggatt ttgggtggtttgtgagaata tttgcattat 217740 gtatacttac cagttgagta tcccaaatct gaaatccttgaatgtcatgt cagtgctcaa 217800 aaagtttcag aatttggagc attttggatt tttggattttccaattaagg atgcctaacc 217860 tgtactatag tgacctcttg tgtactcatt aagcttcaaaaaccttcatt tggtagccag 217920 tcgtgtttca ttttgtacct ccacctcccc ttcccaatcctctgacactg aattattttg 217980 aagctcatat caggcaccac attgtccttt tgtaaatacatcctaatttg taaatatttc 218040 attatcttaa agatagggac tcttttttaa aaagacaaacataactacaa tgccattatc 218100 aaaggtagaa aataggtgac agttatataa tattataaaatatctaatta gtgcttaagt 218160 tcctctagta gtctcataat ttgttcttat tgcatttattttgcttgaat ttggatccaa 218220 agtttggaca ttgcatttca ttaatacgtc ccttaagtttattttaatct gtattttcct 218280 cctccctttt gtgttctttg taatctcttt ttgctgttgttttcggttaa agaaaccatg 218340 tttttttcgt cctgtgagtg gctcctgttc agaattttactgatttcatc tgctggtatc 218400 atttagcatg ttgctctgtc cgccgtagta ctttaaactagacgttagat ctagagatgt 218460 gatctacttc ggtaggactt tgtcaagaat acttgtaagtagtatttagg taccaggaga 218520 cacataaaca taaaatctgg gctgggtgca gtggctcacacctgtaaaac caacactttg 218580 ggaggctgag gtgggtggat ctcttgagcc caggagtttgaggccagcct gaacaacatg 218640 acgaaacctc atctctacaa aaaataaaaa attagcgaggtatggtggca tgcacctgta 218700 gtcctagcta cttaggaggc tcaggtggag gattgcttgagcccaggaga tggaggttgc 218760 agtgagctga aatcgtgcca ctgtacttca gcctgggcaacagaacaaga ccctgtctca 218820 aaaacaacaa caaacaaacg aatgaaaaaa cataaagtctggctgtatct ttttgtggtg 218880 ttagtagcca ttgatgatca ttgcctaggt cctttgtttctttaggaatt tgcagtggtg 218940 atcatctatt tttatccatc ttccttcatt tgttaattggaatactactc tagagatgaa 219000 catttcctca tcaattatat agtagttact ttgaggtacagcttataaaa gaaagtcagg 219060 ttaaatactt aattctttgt cagtttttgg aacagtgagttatttcccag gcatcctcca 219120 aagatgatta gtgaattttt aaaatatatt tgtgaactcatgaattttaa tatatttaat 219180 atatgttagc cctttgcatt attcttaatg atgctcacatgaagtttagt aggaattctt 219240 taagtttaga cactacccta gtagttttag atcacatccttgctttttga tatgcataga 219300 ttttccagaa ttattttttg tcccagacct gaaatcacccatttgcctag gaactccggt 219360 tccttttgta gcatatgaca ttcggggtct ataacctgaattctatgggt gctcactact 219420 gttgggttgg tcaatgtttg taggtgattt taatggacagaggtaggaaa caattttttt 219480 tgtaattaat aatttcttat tgtgatcagg aactattaaaagatttctac tttgggattt 219540 ctacttttaa tgattttttt tctggcttag tttataaatgatttttgcat ttcactgtta 219600 catgggcatt tgaaaattgt cgatttttgt atgaaacatctttttgggtg aaaagttgat 219660 ccgttttcta atgtaatcca tcagttttct aagattgcatttattctttg tccctttgtt 219720 ctgtcaaatt ttaaaatgtg ttttaaaagt tttcactatcattgtgggtt ttttctcata 219780 gcctcccatg tatttctttt cttttttttt ttttgagacggagcctcact ctgtcaccca 219840 ggctacagtg cagtggcatg atctcggctc attgcaacctctgcctccca ggttcaagtg 219900 gttctcctgc ctcagcctcc caagtagctg ggactacaggcatgcgccac catgcccagc 219960 taatttttgt atttttagta gagacagggt ttcaccatgttggccaggat gtcttgatct 220020 cttgacctcg tgatctgcct gcctcagcct cccaaagtgctgggattaca ggcgtgagcc 220080 accatgcctg gcagcctccc ttgtatttct aacagcttttgctgtctata ttttttaatt 220140 aaggtgaaat tcttgtaaca aaaagctaac catttaaaaatatacaattc aacagcattt 220200 actacattca aaatgttcac aaatacctcc tctatgtggttccaaaacat ttcattttga 220260 aactacaaaa taggatccat taagctgccc ctccctattctccgctccac tatctcctgg 220320 caaccaccaa tttcctttct gtctctgaat ttacccgttctggatatttc gtataaatgg 220380 catcatagaa tgtgtgacct tttgtgtcag gcttttttcacttagcataa tgttttacag 220440 gttcatctat attgtagcat gtgtcagtag ttcattcatttgtggctgag taatattctg 220500 ttatatgtat ttaccacatt ttgcttagcc attcatctgttgatgatata catataacag 220560 aatattactg tggattgatt ccaccttttg gctattgtaaatagcactcc ttcaacattt 220620 gtgtacaagt atttgttgga gtacctgttt ttaattctttaggggtgtat atttaggagt 220680 ggaatttctg agtcatcttg taattctatg tgtaactttggaggaatttc caaactgttt 220740 tctgcaatgg ctgcaccatt ttacattcct gtcagcaatgtacaggattc cagtttctcc 220800 atatccctgc caacacttgg tattttcctt tttttcagttagttactcta gtgggtatgt 220860 ggtggttgtc ccattgctgt ttgttttgat tttcattgtccctaatgact aatgatattg 220920 agcatctaat atccagttgt cccagcaaca tttgtttgaagagactattc tttcctgtgt 220980 aaatggtctt ggcatctttg ttgaaaatcg attggcagccaggtgcagta gctcacacct 221040 gtaatcccag cactttggga ggctgcgctg ggcagatcgcttaagctcag gagtttgagt 221100 ccagcctggg caacgtggca aaaccatgtc tctactaaaaatacacaaat tagccaggca 221160 tgatggcacg tgcctgtagt cccagctact tgggggactgaggccggagg atcacttgag 221220 cctgggaggc agcggttgca gtgagccaag attgcaccattgcactccag catgggcgaa 221280 cacagtgaga ccctgtctcc aaaaaaaaaa aagaaaatcaattgactata gatatcatgc 221340 atctatgata ttctggattc tcaaccctat tccattggtctgtgtgtttg tccttatgcc 221400 agcatgtacc acactgtttt gttttgttca ctgtagctttgtagcaagtt ttgaaatcag 221460 gaagtttgaa ccctccaagt ttgttgttct tcagtatgttttacgtattc agggttcttt 221520 gcaattctat gtgaatttga ggattggctt ttctatttctgcaaacaaga cctttagaat 221580 tttgatagca atttctttga atctgtagat tactttgtgtagtattgcca tcttaacaat 221640 attaaatctt ccagccaggc gccagtggct caagcctgtaatctcagcat tttgggaagc 221700 caaggtaggc agatcacttg agcacaggag tttgagacccccccgagcaa catggcgaag 221760 cctgtctcta caaaaaataa aaaatcagcc aggtgtggtggtgcacgcct gttgtcccag 221820 tgctccagcc tggctgacag aggcagacct tgtctcaaaaaaaaccaaaa ctaatattaa 221880 gtcttccaat ccatgaacaa ggatatattt tcatttatttagatctttta aaatgtgttt 221940 cagcagtgtt tttgttcatt tgtgtgtttg tttttagttttctatataca aatttcttcc 222000 tgggtatttt attctttttg atgctcttaa agggaattttctcaattttc tttttggatt 222060 gttcattgag tatgatgtta gctgtgggta tttcatgaataccttgcttt acatattttt 222120 aatgcagtgt tttttggtgt gtaagatttt gactcctgtatcttctgggt ggattaaaat 222180 ttgtattata aatgatatta aatgtatctg actctgaggctttttcttaa tgtgcatttt 222240 cttcttatat cttgcccatt aatttatttt taatttttgtatgttcttgg tgggactcaa 222300 tgcctttttg tagattatgc ctttttgtaa attttatattttattttggc attttaacat 222360 atttaaatta gttttttgct aaaacaatga tatttcagcaaatggtattt gtcatgatga 222420 atgaactttt taatgtattg tggaatttgg tttgctagcatcttgttgag ggtttttgca 222480 tgtgtgctcc tcgtgaatat tggcttatag cttttttgtttatttgtctt ttaaaatgtg 222540 tcttcatctg gttttggtat caaggtaata ctggccttgtagaatgagtt tggaaacatt 222600 ctctcctcct ctattctttg aaatagtttg agtaggattgttatttgttc atctttaaat 222660 gtttggtaag ggccggatac ggtggctcac gattgtaatcccagcacttt gagaggccaa 222720 ggtgggcgga tcacttgagg tctggagttc cagaccagcctggcctagcc aacatggaga 222780 aaccccatct gtactaaaaa tacaaaatta gctgggcatggtggtgcatg tctataatcc 222840 cagctactcg ggaggctgag gcaggagaat cacttgaacccgggaggcgg aggtggcagt 222900 gagccgagat tgcatcactg cactccagcc tgggtgacagagcaagatcc tgtctcaaaa 222960 aaaaaaaaaa aaaaaaaaaa agtttggtaa aatttagcagtaaagtcatt cagtcctggg 223020 cttttatttg ctggaagact ttttattaca gcttagatctcattacttgt tatttgtctg 223080 tttaggtttt ggatttcttc atggtttaat cttggtaggttgcatgtgtc tgatctttat 223140 tatttatttt cttctactaa ttttgggttt gatttgctctggcttttcta gttctttaag 223200 atgtatcatt aagttgttta ttgaaacttt ttctacctttttgatgtaga tgctcagagc 223260 tataagcttt cttgtactgc tttcactgta tcccattggcttttgtatgt tttgttttca 223320 ttatcatttg tttcaggaaa tattttaatt ttcttcttaatttcttcatc aatccgctgg 223380 tcattcagga gcatattctt taatttccag gtatttgtatcgtttcaaaa attccgcttt 223440 ttggccaggc atgatggctc atgcctgtca tcccagcactttgggaggcc gaggcgggtg 223500 gatcacttga ggtcagaagt tcgagaccag cctggccaacatggtgaaac cctgtctcta 223560 ctaaaaatac aaaaattagc caggagtggt tgcaggcacctgtagtccca gctagttagg 223620 aggctgaggc aggagaatcg cttgaacccg ggagtcggaagttgcagtga gccaagatca 223680 cgccatttgt actccagcct ggggtacaag agcgaaacttcgtctcaaaa aaaaaaaaaa 223740 aatccacttg ttgttgattt ctagttttgt ttcattgtggtcagagaaga tagatacatg 223800 atataatttt actttttttg aatttttcaa gacttgctttgtggagtaac atatggtctg 223860 tccttgagaa tgatcctcat gctaaggaga atgtatattctgtggctgtt ggatgaaatg 223920 ttctgtaaat atttatgagg tcccatttgg tctatagtgcagattaaatc tgatttttct 223980 ttgttgattt tctgtctagg tgatctgtcc agtgctgaaagtgaggagtt gaagtctcca 224040 ggattggggt ctatccctgt ctttagctct aacaatatctgctttatata tctggttgct 224100 ccagtgttgg gtgcatctgt atttacaatt gttatattctcttgctgaat tgaccccttt 224160 atcattacat tatgaccttt tctgtgcctt tttatagtttttgtcttgaa atctattttg 224220 tctgatacaa gtataactgc tactgctctt tttttgtttttgtttttgtt ttaattggca 224280 tggaatatct gtttccatcc ccttgttttt tctgtgtatgtctatgtgta cctttataca 224340 tgaagtgtgt ttcttgtagt caacagatca ttgggttctgtttttttgtt tttttatttg 224400 tttgtttttt ggtccattca gccactccat gtctttcgattgggcagttt agttcatctg 224460 cattcactgt tattgttaat aagtagggac atactcctgccattttgtta tttttttgtg 224520 attgttttgt ggtcttctct tccttctttc cttcctgtttctgcttaatg aacatgattt 224580 tctctggtga taggttttaa tttcttgctg cttattttttgggtatctat ctgttgtatg 224640 tcttttgatt tgaggttacc atgaggcttg caaataatatcctctaaccc gttattttaa 224700 actgatgatg acttaacagt gatggcatta acaaactaacaaacaagcaa agagaaaact 224760 aatacaaact ctacacttta acttagtccc ctgcatcttaacttttcgtt gcttctattt 224820 ataatcttac tgcctcttgg aaagtattat agttgttatttttgatcagt tcttttagtc 224880 tctctaccca agatatgaat aatttataca ctgcaattgcagtgttacag tagtgttttt 224940 cagtgtactt accattaccc tatggccacc accctggaactgtgctgagt cagacctctg 225000 aacccttaga accttcttgt gcttgaatat tggtatctttttctagattt gggaagttct 225060 gtattattac cctttgagta aactttctac ccctatctctctttctgcct gctctttaag 225120 gctaataact cttagatact ctcttttgag gctattttctagatcttata tgcatgttcc 225180 attcttttta attctttttt gtctcctatg actgtattttcaaatagtct gtcttcaagc 225240 tcactaatac tttcttttgc ttgaacattt ctgctggtaagagaccctga tgcattcttc 225300 agtatgtcgg ttgcattttt caactccgga atttcagcttgattctttgt acttatttca 225360 gtctttttgt taaatttatc tgatgggatt ctgaattccttctttgtttt atcttgaatt 225420 tctttgattt tcctcaaaac agctatttta aattctctgtctgaaaggtc ttatatctct 225480 gtctctccag gattggtccc tggggcctta tttagtttctttggtgagat tatgttttcc 225540 tgaatggtcg tgatgtttgt ggttgtttgt tggtgtctgggcattgaaga gttaggtatt 225600 tattgtagtc attgcagtct gggcttgttt gtacctgtcatttttggaaa ggctttccag 225660 gtattcgaag ggacttaggt gttgtgattt aagtttttggtcactgtagc catatctgta 225720 ttaggaggca tccgaagccc agtaatactg tggctcttgcagactcttag aggaaccatg 225780 ttggtggttt tgggtaagat gtggaagaat tatctgtattataatgcaga ggctctgttc 225840 tcttcccttt ctcccaaaca aatggagtct ctctgcgctgagctgccagg gagagggtgg 225900 ggaggggtta cacaatcacc cttgtagcca ccaccactggaactgtgctg ggtcagacct 225960 gaagccagca cagcactggg tctcaatgct gccaggcttgggactctccc ttcagggcag 226020 tgggctcctc tctggcccag agcaggtcca gaaatgccatccaagaacca ggacctggaa 226080 ttggggaccc ccaagagcct gcttggtgct gtaccccaccatggccaagc tgatatatat 226140 cactaagctg attgttggtt cttatgaagg tgcttttctgtgcgaatggt tgttcaaatt 226200 ggtgttcctg cagagaggac aatcggtgga ggcttctatttggcccttct ctgcctcccc 226260 ctgttttatt tttaattgac acataataat tgtacatacttatgggatgt tttggtacgt 226320 atatgcaatg tgtaatgatc aaattagggt aattagtatgtacatctcct caaacatttg 226380 tcatttctct atggtgagaa cattcaaaat catctcttctagctattttg aaatatgcag 226440 tatgttattg ttaactatag tcagtctact gcatggtgggaacattttca ctcttcagtg 226500 ctgcttttgg ataagcaaaa ggtcttaatt tcttgtatttgatgtagtcc agtgtatcta 226560 gttttttctt tattgttagt gtggttttta gtgtggcctatcgaaaaagt ctttgactag 226620 attatgaaga taatatcttc tattagcttt tagaaattttgttttaatca acttagatta 226680 tgatacctag tgtttgggcc cttattcctc ccattatgtttttatatttt ctgtatataa 226740 agcttctttg ctttcgtgta ttgcacaggt aagttctagtttatttccga catttctttt 226800 cagacagagt aaatgaggaa aataaactat acttaatttgtctctttcat gtggttgcta 226860 ggaatcttgc gtggattcaa cccatatagt ttattatcttgtctccttag gagttggttt 226920 gcagtaagct gcttctacat gaaaaattca aagattaatctgaattacta tctttgtaca 226980 gattttttaa aatgattttt aaaacagatt ttctcctagaactcctgggc tcaggcgatc 227040 ctcccacctc agtctcccaa gtagctggga ctatagacttgtgtcaccac acccagctga 227100 tttttgtatt tgagacaggg tttcaccatg ttggctaagctgatctcaaa ctcctgagct 227160 caggcaatct gcctgcctca gcctcccaaa gtgctgagattacaggcatg aaccactaca 227220 cctggcctat tttaaagata attatatcat ttattttttaaattataatt tctaaatagc 227280 acttcaacat ttaaacatct tagcacattt gctttatctctgtccacaca aacacacata 227340 tactctgtat ttttggtcaa ttatttgaaa gaaagcggcagctgtttctt tctcctacat 227400 gaacttactg caacatgaaa atatcttgag tgtgtaaataaaattattta tttcattgtt 227460 ttctagatct agaactaatc tagcagatat gcattatcaggtgccaaaga tttaatattt 227520 atttgtttat taaataacta ctttagaaac cctgaaatatgggaggtgac tagagtacat 227580 ttgagagtgg gacaagtaca gtaagtcaaa ctgtactttgagtagtacag aagttttact 227640 ggcccatctg gtacttcatc tattttcctt gtcaaatcctaggccacttt gaatcacaaa 227700 atatgaaaat ataattttta ttttaagcta gacaatatattaagtcagat actacatgtg 227760 ttaaatttgt gtgtttatta aatatttatg ccttaaatacctgtaaggtt aaagcatgtt 227820 ttaatgagta gacaattact aaatatatga gttactaccattgcaaggca cgaacatcag 227880 gtaagttaaa actaagcttt gttagagtac ttgaattccctcaaaaactt agttttnctg 227940 tgaaaataaa agtttggctc cannnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 228000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 228060 nntctaagaa agggaatgtt gaactgtgtg acttgaatgcagatatcaca aagcagtttc 228120 tgagagtgcc tctgtctaga ttttatatga aggtattcccgtttccaaag aaatcattag 228180 agctatgcaa atatccactg gcagattcta caaaaagagtgtttccaaac tgctgtatca 228240 aaagacagga tgtaccgccg ttagttgagg acacacatcacaaagaagtt tctgagaatg 228300 cctctgtcta gattttatat gaagatattc ccgtttccaatgaattcctt aaagctctcc 228360 aaatatccac ttgaagattc tccaaaaaca gtctttcaaaactgctctgc aaataaaatg 228420 tttcaactct gttagttgag gacaaacatc acaatccagtttgtgagaat gcttctgtct 228480 agtttttatg ggaagatatt tcttttgcac cttaagcgtcaaagcgctcc aagtgtccac 228540 atccagatag tacagaaaga gtgtttcaaa cctgctctatgaaagggaat gatcaactct 228600 gtgacgtgaa tgcagacatc atatagcagt ttctgagaatgtttctgtct aggttttata 228660 tgaagatact cccgtttcca aggaaatcca caaagctatccaaatatcca cttgcaaatt 228720 ctacaaaaag cgtgtttcca aactgctctg tcaaatgaaatgttcaactc tgatagttga 228780 ggatacatat cacaaacaag tttctgcgaa tgcttctgtctagtttttat cggaagatat 228840 ttcctttttc accataggcc tgaaagcact cgaaatgtccacttccagat actacagaaa 228900 gagtgtttca aaactgctct atgaaaggga atgttgaacactgtgactta aaagcaaaca 228960 tcacaaagaa gcttctgaga atgctgcttt ctatttttatatttaatccc ttttccaacg 229020 aaatcctcaa agctatccaa atatcctcct gcagattccacgaaaagacg gtttcaaacc 229080 tgctctaaga aagggaatat tcaactctgt gacttgaatgcagatatcag aaagtagttt 229140 ctgagagtgc ttgtgtctag attttatatg aagatattcccgtttccaac gaaatagtta 229200 gagctatcca aatatccact tgcatattct acaaaaggagtgtttccaaa ctgctgtatc 229260 ataagacagt ttgaactctg ttagttgagg acacacatcacaaagaaatt tctgagaatg 229320 cttctgtcta gtttttatgg gatgatattt cctttttcaacataggactg aaatcgcccg 229380 aaatgtccac ttccggatac tacagaaaga gtgtttcaatcctgctctat ggaagggaat 229440 attcaactct gtgacttaaa agcaaccatc acaaagaagcttctgagaat gctgctgtct 229500 atattttata tgaaatcccg tttccaagga aatcctcagagctatccgaa tatccacttg 229560 cagattacag aaaaagagct tctcaaaact gacccagaaatagaaaggtt caactctgtt 229620 agttgagtac atatatccca aagaagtttc ttagaatgcttctgtctagt ttttatggga 229680 agacatttcc tttttcacca aaggggtcga agcggtctaagtgtccactt acacatacta 229740 caaaaagagt gtttcaaacc ttctctaaga aagggaatgttcaactctgt gacttgaaag 229800 cagatagctc aaagcacttt cggagagtgc ctctctagattttatatgaa ggtattccca 229860 tttccaacga aatcgttaca gctatcgaaa tatccacttgcagattctac aaaaagagtg 229920 tttccaaact gctgtatcaa aagataggtt gtactctgttagttgaggac acacatcata 229980 aataagtttc tgagaatgcc tctggctaga ttttacctgaagatattacg gtttaaaatg 230040 aaatccttga agctctccaa atatccgctt acagattctccaaaaagagt ctttcaaaac 230100 tgctctgtaa atagaaaggt tcaacgctgt tagttgaggacatacatcac aaaccagttt 230160 gtgagaatgc ttctgtctag tttttatggg aagatatttccttttttacc gtaagcgtca 230220 atgtgctcca agtgtccaca tccagatact acagaaagagtgtttcaaac ctgctctatg 230280 aaagggaatg ttcaactctg tgacgtgaat gcagacatcacaaagcggtt tctgagaatg 230340 tttctgtcta atttttatat gaggatactt ccgtttccaacgaaatccac aaagctatcc 230400 aaatatccac atgcaaattc tacaaaaagc gtgtttccaaactgctctgt gaaacgaagt 230460 gttccactct gtgagttgag ggcacacatc acaaacaattttctgctaat gcttctgtct 230520 agtttttatg ggacgatatt tccttgttca ccataggcctgaaagcgctc gaaatgtcca 230580 cttccagata gtagagaaag agggtttcaa acctgctctatggaagggaa tattcaactc 230640 tgtgacttaa aagcaaacat cacaaagaag cttctgagaatgctgctgtc tactttttat 230700 atgcaatccc gtctccaacg aaatcctcag aactatccttatatccaatt gcagattcca 230760 caaaaagagc ttttcgaaac tgatctatca atagaaaggttcaactctgt taggtgagta 230820 catatatcgc caagaagttt cttagaatgc ttctgtctagtttttatgga agatatttcc 230880 tttttcacca aaggcgtcaa agcgctccaa atgtccacttccagatacta caaaaagagt 230940 gtctcaaacc tgctctaaga aagggaatgt tcaactctgtgacttgaacg cagatatcac 231000 aaagtagttt ctgagagtgc ctctgtctag attttatatgaagttattct cgtttcctat 231060 gaaatggtta gagctatcca aatatccaca tgcagattctacaaaaagag tgtttacaaa 231120 ctgctgtatc aaaagaaagg ttgaaccctg ttagttgaggacacacatca caaagaaatt 231180 tttgagaatg cttccgtcta atttttattg gaagatatttcctttttcaa cctaggcctg 231240 aaatcgctcg aaatgtccac ttccagatac tacagaaagagtgtctcaaa cctgctctat 231300 ggaagggaat attgaactct gcgatttaaa agcaaacatcacaaagaagc ttctgagaat 231360 gctgctgtct actttttata cgtaatcccg tctccaacgaaatcctcagt gctatcctaa 231420 tatccacatg cggattccac aaaaagagct tttcaaaactgatctataaa tagaaaggtt 231480 caactgtgtt agttgagtac atatatccca aagaagttccttagaatgct tctgtctagt 231540 ttttatggga agacctttcc tttttcacca gaggcgtcaaagcgctccaa atgtccactt 231600 ccagatacta caaaaagggt gtttcaaacc tgctctaagaaagggaatgt tcaactctgt 231660 gacttgaatg cagatatcac aaagcagttt ctgagagtgcctctgctaaa ttttatatga 231720 aggtattccg gtttccaacg aaaccgttag agctatcgaaatatcctctt ggagtttcta 231780 caaaaagagt gtttcgaaac tgctgtatca gaagacaagttgtactctgt tagttgagga 231840 cacacatcac aaagaagttt ctgagaatgc ctctgtctagatttttcctc aagatattcc 231900 ggtttccagt gaaatcctta aagctctcca aatatccacttgcagagtct ccaaaaagtg 231960 tctttcaaaa ctgctctgta aatagaaagg ttcaagtctgttagttgagg acatacatca 232020 caaaccagtt tgtgagaatg cttctgtcta gttttcatgggaacatattt cctttttcac 232080 cgtaagcgtc aaagccctcc aagtgtccac atccagatactacagaaagg gagtttcaaa 232140 cctgctctat gaaagggaat gttcaactcc gtgacgtgaatgcagacatc acaaagcagt 232200 ttctgagaat gtttctgtct aggtttaata ggaagatactcccgtttcaa gcaaaatccg 232260 caaagctatc caaatatcca cttgcagatt ctacaaaaagcgtgtttcca aactgctctg 232320 tcaaacgaaa tgttcgactc tgtgagttga ggacacacatcacaaagaag tttctgcgaa 232380 tgcttctatc tagtttttat gggaagatat ttccttgttcaccataggcc tgaaagcgct 232440 cgaaatgtcc acttccagat actacaggaa gagtgtttcaaacctgctct atgaaagaga 232500 atgttcaact ctgtgactta caagcaaaca tcacaaagaagcttctgaga atgctgctgt 232560 ctactttgta tatgtaatcc cgtttccaac taaaccctcaaggctatcga aatatcctcc 232620 tgcagattcc acgagaagac ggtttaaaac ctggtctaagaaagggaatt ttcaactctg 232680 tgacttgaat gcagatatca caaagtagtt tctgagagtgcttctgtcta gattttatat 232740 gaagatattc ccgtctccaa cgaaatactt agagctatccaaatatccaa ttgcatattc 232800 tacaaaaaga gtgtttccaa actgctgtat cataagaaaggttgaactct gttagttgag 232860 gccacacatc acaaagacgt ttctgagaat gcttctgtatagtttttatg agaagatatt 232920 taatttttca acataggcct gaaagcgctc gaaatgtccacttccagata ctacagaaag 232980 attgtttcaa acctgctcta tggaagggaa aattcaactctgtgacttaa aagcaaacat 233040 cacaaagacg cttctgagaa tgctgctgtc tactttttatatgtcatccc gtctccaacg 233100 aaatcctcag agctatttta atatccactt gcagattccacaaaaagagc ttttcaaaac 233160 tgatctataa agagaaaggt tcaactctgt tagttgagtacatatatccc aaagaagttt 233220 cttagaatgc ttctgtctag tttttatggg aagacatttcctttttcacc aaaggcgtca 233280 aagccctcca aatgtccact tccagatact acaaaaagagtgtttcaaac ctgctctaag 233340 aaagagaatg ttcaactccg tgacttgaat gcagatatcaaaaagcagtt tctgagagtg 233400 cctctgtcta gaatttatat gaagttattc ccgtatccaacgaaatcgtt acagctatcc 233460 aaatatccac ttgcgtattc tgcaaaaagc gtgtttccaaactgctctgt caaacgaaat 233520 ggtcacctct gtgagatgag gacacacatc acaaacaagtttctgcgaat gcttctgtct 233580 agtttttatg ggacgatatt tccttgttca ccataggcctgaaagcgctc gaaatgtcca 233640 cttccagata gtagacaaag agggtttcaa acctgctctatggaagggaa tattcaactc 233700 tgtgacttaa aagcaaacat cacaaagaag cttctgagaatgctgctgtc tactttttat 233760 atgcaatccc gtctccaacg aaatcctcag aactatccttatatccaatt gcagattcca 233820 caaaaagagc ttttcaaaac tgatctatca atagaaaggttcaactctgg tagttgagta 233880 catatatcgc aaggaagatt cttggaatgc ttctgtctagtttttatgga agatatttcc 233940 tttttcacca aaggcgtcaa agcgctccaa atgtccactaccagatacta caaatagatt 234000 gtctcaaacc tgctctaaga aagggaatgt tcaactctgtgacttgaatg cagatatcac 234060 aaagcagttt ctgagggtgc ctctgtctag attttatatgaaggtattcc cgtttcctat 234120 gaaatggtta gagctatgca aatatccact tgcagattctacaaaaagag tgtttataaa 234180 ctgctgtatc ataagaaagg atgaactctg ttagttgaggacgcacatca caaagaaatt 234240 tttgagaatg cttccgtcta gtttttattg gaagatatttcctttttcaa cataggcctg 234300 aaatcgctcg aaatgtccac ttccagatac tacagaaagagtgtttcaaa ccttctctac 234360 ggaagggaat attgaactct gcgatttaaa agcaaagatcacaaagaagc ttttgagaat 234420 gctgctgtct actttttata cgtaatcccg tctccaacgaaatcctcaga gctattcaaa 234480 tatccacttg cggattccac aaaaagagct ttttaaaactgatctctaaa tagaaaggtt 234540 caactctgtt agttgagtac atatatccca aagaagtttcttagaatgct tctgtctagt 234600 ttttatggga agacatttcc tttttcacca aaggcgtcaaagcgctccaa gtgtccactt 234660 ccagatacta caaaaagaga gtttcaaacc tgctctaagaaagggaatgt tcaactctgt 234720 gacttgaatg cagatatcac aaagcagttt ctgagagtgcctctgtctag atttcatatg 234780 aaggtattcc cgtatccatc gaaatcgcta gagctatccaaatatcctct tgcagattct 234840 acaaaaagag tgtttccaaa ctgatgtatc aaaagacaggttgtactctg ttagttgagg 234900 acgcacatca cgaagaagtt tccgagaatg cctctgtctagattttacct gaagatattc 234960 cggtttccag tgaaatcctt aaagatctcc agatatccacttgcagattc tccaaaaaga 235020 gtctttgaaa actgctctgt aaatagaaag gttcaactctgttagttgag gacatacatc 235080 acaaaccagt ttgtgagaat gcttctgtct agttttcatgggaagttatt ccttttttca 235140 ccgtaagcgt caaagcgctc caagtgtcca catccagatacttcagaaag agtgtttcaa 235200 acctgctcta tgaaagggaa tgttcaactc tgtgacgtgaatgcagacat tacaaaggag 235260 tttcggagaa tgtttccgtc taggttttat atgaagatactcccgttccc aaggaaatcc 235320 acaaagctat ccaaatatcc acttgcagat tctacaaaaagcgtgtttcc aaactgctct 235380 gtcacaggaa atgttcaact ctgttggttg aggacacacatcacaaacaa gtttctgcga 235440 atgcttctgt ctagttttta tggggagata tttcctttttcaccatgggc ctgaaagcgc 235500 tcgaaatgtc cacttccaga tactacagaa agagtgtttcaaacctgctg tatgaaaggg 235560 aatgttcaac tctgtgactt agaagcaaac atcacaaagaagcttctgag aatgttgctg 235620 tctactttgt atatgtaatc ccgtttccaa cgaaatcctcaaagctatcc aaatatcctc 235680 ctgcagcttc cacgaaaaga cggtttcaaa cctgctctaagaaagggaat attcaactct 235740 gtgatttgaa tactgatatc acaatgtagt ttctgagagtgcttctgtct agattttata 235800 tgaagatatt cccatttcga acgaaatagt tagagctgttcaaatatcca cttgcatatt 235860 ctacaaaaag tgttttttca aactgctgta ttataagagaggttgaactc tgttagttga 235920 ggacacacat cacaaagaag tttctgagaa tgcttctgtcttgtttttat gggaagatgt 235980 ttcctttttc aacataggcc tgaaatcgct cgaaatgtccacttccagat actacaaaaa 236040 aagtgtttca aaccggctct atggaaggga atattgaactctgtgactta aaagcaaaca 236100 tcacaaagaa gtttctgaga atgctgctgt ctactttttatatgtaatcc cgtttccaac 236160 gcaatcctca gagctatcct aagattcact tgcagattccacgaaaagag cttttcaaga 236220 ctgatctata aatagaaagg ttcaactctg ttagttgagtacgtatatcc caaagaattc 236280 tcttagaatg cttccgtcta gtttttatgg gaagacatctctttatcacc aaaggtgtca 236340 aagcgctcca gaagtccact tcctgatagt acaaaaagagtgtttccaaa ctgctgtatc 236400 ataacaaagg ctgaactctg ttagttgagg acacacatcacaaagaagtt tctgagaatg 236460 cctctgtcta gattttacct gaagatattc cggtttacaatgaaatcctt aaagctctcc 236520 aaatatccac ttgcagattc tccaaaaaga gtctttcaaaactactctgt aaatagaaag 236580 gttcaactct gttagttgag gaaatacatc acaaactagtttttgagaat gcttctgtct 236640 agttttcatt ggaagatatt tccttttgca ccgtaagcctcaaagcgctc caagtgtcca 236700 catccagata ctacagaaag agtgtttcaa acctgctctatgaaaggaaa tattcaactc 236760 tgtgacgtga atacagacat cacaaagcag tttctgagaatgtttctttc taggttttat 236820 atgaagatac tcccgtttcc aaggaaatcc acaaagctatccaaatatcc acttgcagat 236880 tctacaaaaa gcgtgtttcc aaactgctct gtcaaacgaaatgttcaact ctgtgagttg 236940 aggacacaca tcacaaacaa gtttctgcga atgcttctgtctagttttta tcggaagata 237000 tttccttttt cacgatagtt cggaaagcac tcgaaatgtccacttccaga tactacagaa 237060 agagtgtttc aaaactgctc tatgaaaggg aatgttcaactctgtgactt aaaagcaaac 237120 atcacaaaga agcttctgag aatgctgctt tctactttgtatatgtaatc ccttttccaa 237180 cgaaatcctc aaagctatcc aaatatcctc ctgcacattccacgaaaaga cggtttcaaa 237240 cctgctctaa gaaagggaat attcaactct gtgacttgaatgcagatatt agaatgtagt 237300 ttctgagagt gcttgtgtct agattttata tgatgatattcccgtttcca acgaaatagt 237360 tagagctatc caagtatcca cttgcatatt ctacaaaaggagtgtttcca aactgctgta 237420 tcataagaca gtttgaactc tgttagttga ggacacacatcacaaagaaa tttctgagaa 237480 tgcttctgtc tagtttttat gggatgatat ttcctttttcaacataggac tgaaatcacc 237540 cgaaatgtcc acttccggat actacagaaa gagtgtttcaatcctgctct atggaaggga 237600 atattcaact ctgtgactta aaagcaaaca tcacaaagaagcttctgaga atgctgctgt 237660 ctacatttta tatgaaatcc catttccaag gaaatcctcagagctatccg aatatccact 237720 tgcagattac agaaaaagag cttctcaaaa ctgacccagaaatagaaagg ttcaactctg 237780 ttagttgagt acatatatcc caaagaagtt tctgagaatgcttctgtgta gattttacat 237840 gaatgtagac ccatttccaa cgaaatcctt aaagccatccaattatccac ttgcagatac 237900 tacaaaaaga gtttttcaaa actgctgtgt agaaagaaaagttcagctct gtcagttgag 237960 tacacacagc acaaacaagt ttctgagaat gcttctgtctagtttttatg ggaagatatt 238020 tcctttttca ccaaagtcgt caaagcgctc caaatgtccacttccagata caacaaaaag 238080 agtctatcaa accagatcta cgaaagggaa tgttgaactctgtgacttga atgcagatct 238140 cacaaagtag tttctgagag tgcctctgcc tagattttatatgaagctat tcccgtttcc 238200 aacgaaatcg ttagagctat cgaaatatcc acttccagattctacaaaaa gagtgtttcc 238260 aaactgatgt atcaaaagac aggttgtact ctgttagttgaggacgcaca tcatgaagaa 238320 gtttctgaga atgcctctgt ctagatttta cctgaagatattccggtttc caatgaaatc 238380 ctaaaagatc tccagatatc cacttgcaga ttctccaaaaagagtctttg aaaattgctc 238440 tgtaaataga aaggttcaac tctgttagtt gaggacatacatcacaaacc agtttgtgag 238500 aatgcttctg tctagttttc atgggaagtt atttcctttttcagtgtaag cgtcaaagcg 238560 ctccaagtgt cgacatccag atacttcaga aagagtgtttcaaacctgct ctatgaaagg 238620 gaatgttcaa ctctgtgacg tgaatgcaga cattacaaaggagtttcgga gaatgtttcc 238680 gtctaggttt tatatgaaga tactcccgtt tccaacgacatccacaaagc tatccaaata 238740 tccaattgca gattctacaa aaagcgtgtt tccaaactgctctgtcacag gaaatgttca 238800 actctgttgg ttgaggacac acatcacaaa caagtttctgcgaatgcttc tgtctagttt 238860 ttatggggag atatttcctt tttcaccatg ggcctgaaagcgctcgaaat gtccacttcc 238920 agatactaca gaaagagtgt ttcaaacctg ctgtatgaaagggaatgttc aactctgtga 238980 cttagaagca aacatcacaa agaagcttct gagaatgttgctgtctactt tgtatatgta 239040 atcccgtttc caacgaaatc ctcaaagcta tccaaatatcctcctgcagc ttccacgaaa 239100 agacggtttc aaacctgctc taagaaaggg aatattcaactctgtgactt gaatacagat 239160 atcacaatgt agtttctgag agtgcttctg tctagattttatatgaagat attcccattt 239220 cgaacgaaat agttagagct gttcaaatat ccacttgcatattctacaaa aagtgttttt 239280 tcaaactgct gtattatacg agaggttgaa ctctgttagttgaggacaca catcacaaag 239340 aagtttctga gaatgcttct gtcttttttt atgggaagatgtttcctttt tcaacatagg 239400 cctgaaatcg ctcgaaatgt ccacttccag atactacaaaaaaagtgttt caaaccggct 239460 ctatggaagg gaatattgaa ctctgtgact taaaagcaaacatcacaaag aagtttctga 239520 gaatgctgct gtctactttt tatatgtaat ccgatttccaacgaaatcct cagagctatc 239580 ctaagattca cttgcagatt ccacgaaaag agcttttcaagactgatcta taaatagaaa 239640 ggttcaactc tgttagttga gtacttatat cccaaagaattctcttagaa tgcttccgtc 239700 tggtttttat gggaagacat ctctttatca ccaaaggtgtcaaagcgctc caaaagtcca 239760 cttcctgata gtacaaaaag agtgtttcca aactgctgtatcataacaaa ggctgaactc 239820 tgttagttga ggacacacat cacaaagaag tttctgagaatgcctctgtc tagagtttac 239880 ctgaagatat tccggtttac aatgaaatcc ttaaagctctcaaatatcca cttgtagatt 239940 ctccaaaaag agtctttcaa aactactctg taaatagaaaggttcaactc tgttagttga 240000 ggaaatacat cacaaactag tttttgagaa tgcttctgtctagttttcat tggaagatat 240060 ttccttttgc accgtaagcc tcaaagcgct ccaagtgtccacatccagat actacagaaa 240120 gagtgtttca aacctgctct atgaaaggaa atattcaactctgtgacgtg aatacagaca 240180 tcacaaagca gtttctgaga atgtttcttt ctaggttttatatgaagata ctcccgtttc 240240 caaggaaatc cacaaagcta tccaaatatc cacttgcagattctacaaaa agcgtgtttc 240300 caaactgctc tgtcaaacga aatgttcaac tctgtgagttgaggacacac atcacaaaca 240360 agtttctgcg aaggcttctg tttagttttt atgggaagatatttccttgt tcaccatagg 240420 cctgaaagcg ctcgaaatgg ccacttccag atattacagaaagagtgttt gaaacctgct 240480 ctatgaaagg gaatgttcaa ctctgtgact taaaagcaaacatcacaaag aagcttctga 240540 gaatgctgct gtccactttg tatatgtaat cccgtttcctacgaaatcct caaagctatc 240600 caaatatcct cctgcagatt ccacgaaaag acggtttcaaacctgctcta agaaagggaa 240660 tattcaactc tgtgacttga atgcagatat cacaaagtagtttctgagag tgcttctgtc 240720 tagattttat atgaagatat tcccgttacc aacaaaatagttagagctct ccaaatatcc 240780 agttgcatat tctacaaaaa gagtgtttcc aaactgctgtatcataagag aggttgaact 240840 ctgttagttg aggacacaca tcacaaagaa gtttctgagaatgcttctgt ctagttttta 240900 tgggaagata tttccttttt caacataggc ctgaaatcgctcgaaatgtc cacttccaga 240960 tactacagaa agagtgtttc aaacctgctc tatggaagggaatattcaac tctgtgattt 241020 aaaagcaaac atcacaaaga agcttctgag aatgctgctgtctattttta tatgtaatcc 241080 cgtctccaac gaaatcttcg gcggtatcct aacatcgacttgcagattcc acaaaaagag 241140 cttttcaaaa ctgatctcta aatagaaagt ttcaactctgttagttgagt acatatatcc 241200 caaagaagtt tcttagaatg cttctgtcta gtttttatgggaagacattt cctttttcac 241260 caatggcgtc aaagcgctcc aaatgtccac ttccagatactacaaaaaga gtggttcaaa 241320 cctgctctca gaaagggaat gttcaacact gtgacttgaatgcagatatc acaaagcagt 241380 ttctgagagt gcctctgtct agattttata tgaaggtattcccgtttcct acgaaatctt 241440 tagagctatc caaatatcca cttgcagatt gtacaaaaagagtgtttcca aactgctgta 241500 tcaaaagcca ggtagtactt tgttagttga gggcacacatcacaaagagg tttctgagaa 241560 tgcctctgtc tagattttac ctgaagatat tcgggtttccagtgaaatcc ttaaagctct 241620 cgaaatatcc acttgcagat tctccaaaaa gagtctttcaaaactgctct gtaaatagaa 241680 aagttcaact ctgttagatg aggacataca tcacaaaccagtttgtgaga atgcttctgt 241740 ctagttttta tgggaagata tttccttttt cactgtaagcgtcaaagcgc tccaagtgtc 241800 cacatccaga tactacagaa agagtgtatc aaacctgctctatgaagggg aatattcaac 241860 tctgtaacgt gaatgcagac atcacaaagc agtttctgagaatgtttctg tctaggtttt 241920 atatggagat tctcccgttt ccaacgaaat ccacaaagctatccaaatat ccacttgcag 241980 attctacaaa aagcgtgttt ccaagctgct ctgtcaaacgaaatgttcta ttctgtgagt 242040 tgaggacacg catcacaaac aagtttctgc aaatgcttgtgtctggtttt tatgggaaga 242100 tatttccttg ttcaccatag gcctgaaaac actcgaaatgtccacttcca gatactacag 242160 aaagagtgtc tgaaacctgc tctacgaaag ggaatgttcaactctgtgac ttaaaagcaa 242220 gcatcacaaa gaagcttctg agtatgctac tgtctagtttttatgggaag atatttcgtt 242280 tttcaacata ggcctgaaat cgctcgaaat gtccacttccagatactata gaaagagtgt 242340 ttctaaactg ctctatggaa gggaatgttc aactctgtgacttaaaagca aacatcacaa 242400 agaagtttct gagaatgctg ctgtctacat tttgtttgtaatcccgtttc caacgaaatc 242460 ctgaaagcta tccaaatatc cacttgcaga ttccacaaaaagagcattaa aaagctgatc 242520 tataaagagc aaggttcaac tcggttagtt gagtacatatatcacaaaga tgtttcttag 242580 aatgcttctt tctagttttt atgtgaagac atttcctttatcaccaaagg cgtcaaagcg 242640 ctccaaatgt ccacttccag atcctgcaaa aagagtgtttcaaacctgct ctaagaaagg 242700 gaatgttgaa ctgtgtgact tgaatgcaga tatcacaaagcagtttctga gagtgcctct 242760 gtctagattt tatatgaagg tattcccgtt tccaaagaaatcattagagc tatgcaaata 242820 tccactggca gattctacaa aaagagtgtt tccaaactgctgtatcaaaa gacaggatgt 242880 accgccgtta gttgaggaca cacatcacaa agaagtttctgagaatgcct ctgtctagat 242940 tttatatgaa gatattcccg tttccaatga attccttaaagctctccaaa tatccacttg 243000 aagattctcc aaaaacagtc tttcaaaact gctctgcaaataaaatgttt caactctgtt 243060 agttgaggac aaacatcaca atccagtttg tgagaatgcttctgtctagt ttttatggga 243120 agatatttct tttgcacctt aagcgtcaaa gcgctccaagtgtccacatc cagatagtac 243180 agaaaggggg atnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 243240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnttagtttt 243300 ctgtgaaaat agagtttggc tccattaagt ttaaaagtaattttattaaa cactttgcag 243360 tgtctggcac atatggacca cttaatactt gccttaattaactaaaggct ctcaccttta 243420 atgtggttag aaagagcact cagtgccggg cgcggtggctcacacctgta atcccagcac 243480 ttttggaggc tgaagcaggc ggatcacgag gtcaggggattgagaccatc ctggctaaca 243540 aggtgaaacc ccgtctctac taaatataca aaaattagccgggcctggtg gcaggcgcct 243600 gtagtcccag ctactcggga ggctgaggca ggagaatggcgtgaacctgg aggcagagct 243660 tgcagtgagc tgagcttgtg ccactgcact ccagcctgggcaacagagcg agactccgtc 243720 tcaaaaaaaa agcactcagt aaggacttgg atctgatttctagctcaagc tgaggcttca 243780 tctctttggc tacttgtaaa taaggaatat gcactagattatatccaatt agcattaata 243840 tttttattcc atatcttgtg tttattgtga aaacacttaataaaatctta tcggatcagg 243900 aaacttaata cattaaaaaa acatttttta aaaactaaattgtcaatact gaacaaaaag 243960 acttctttca atgactacag acttgaactt ttttctcacttgatagctcg aattacatct 244020 ttacaagagg aggtgaagca tctcaaacat aatctcgaaaaagtggaagg agaaagaaaa 244080 gaggctcaag acatgcttaa tcactcagaa aaggtaaatgatttaacata taccaattat 244140 tataaaatag taattactga ttatcataga ctactaggtggttttataaa atagcattta 244200 ttcttaatgt ttatatagtt acatttctaa aagtttttatgtttgattgg ttgtatgtgg 244260 ggctgtatgt atgtcttatt tagggttttg ttatttgttatttttgtttt taccctagag 244320 agcattagat gtttcatgtt aacagatcat cacagattcctttggtttgg aaataccttt 244380 ttataaagta tgttttccta ttttctttgt acatgagtaagatgaatgtg ctgtgagggc 244440 ttatgaagaa ggaaaacaac agatcagtac gtggtagatgatgagtacct gttctgttgt 244500 tttacagtta gcaaaataga aaagagcata aagtgattggcctttgcatt tagaattttc 244560 ttaacaataa aaatttcatt ggttattctc tgttatatacctagctaaga gttaaataga 244620 tgatatagag atacagctgt cactttttca aagcctctgaaatgttggag gttcaataga 244680 acctcaggta aattatcaga aaagccacta cttatcttgtcctttggaca tgttttgtca 244740 caattagaat agtttttgat ggccaagctg gtgtaaatgtggacatagga taaaagaagc 244800 ttgaagagta gaaaagggta cccaaacaga aatggaatatattttgactc atttttcctt 244860 tctatatcaa tgacttgcca caggatactt tgtggggtttttttgtttgt tttttgtttt 244920 tgtttttttg tgaaagcaaa gtaaatgtgg atatagatgcaaatgtagat aaagatgatt 244980 taatatattt ttttaaagtg gtagcaggcc aggcaaggtggctcatgcct gtagacccaa 245040 cactttggga ggctgacaca ggaggattgc ttgagcccaggaatttgaga atagcctggg 245100 cagtggagcg agaccccatc tctacaaaaa aatgcttaaaaattagccag gtgtggtggc 245160 atatgcctgt aatactagcc actccagagg ctaaggctggaggatagctt gagcccagga 245220 gttcaaggct gcagtgagct gagaaggtgc cactgcactccagcctgggt gacagagtga 245280 gacctcatct ctttaaaaaa aaaagaaaag aaaaaaaaagatggtatcag aaatttttaa 245340 ataaactgaa aatttgtctt attcatttcc gatcttagcaatgaactgtc ctgaacataa 245400 ccaccagcac agccttgttc tgccctccgc catgattaatgattcaaatg ggaagggagg 245460 gggtaaagaa aagattatct tttgctttta attatatgtgtttattgtga tttttaaaat 245520 aaggcatcat atctcttcca gaatattaaa tgtaagatttactaacatgc atgccttctg 245580 cacagtttat tacatgactc ctactcccat tttacaggaaaagaataatt tagagataga 245640 tttaaactac aaacttaaat cattacaaca acggttagaacaagaggtaa atgaacacaa 245700 agtaaccaaa gctcgtttaa ctgacaaaca tcaatctattgaagaggcaa agtctgtggc 245760 aatgtgtggt aagtgtcagt tttaatattt ttacaaaattgactttaaat agtgactaga 245820 aaaaatttgg ttttggtcaa aattttgtac tctttaactcgtagaaagta taatataaat 245880 tataggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 245940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnatgc ctggcaatgc 246000 tttccatttt ttaagatcac aatttcacct tctagttccctcagtttact actctgtttc 246060 ctataaaagt tagtggaatc tgtcttttct ctgttttttagtagcccttt ccttgatttt 246120 ctttatgatc cactctggac ctcatcatct gctaattcatctgctatctt gctttgtacc 246180 ctcagttttc tcatttcttt gtacttccac tgtgttatgcctccaaacac aaccctagtt 246240 cagaccagcc agccactttc ttcaggcagt tgagtgttagtccaatgcat tatctgcaac 246300 ctgagttgtc aatttttttt caatttttaa aaattatttatttttggtag agaaagagtc 246360 tcactatgtt gtccaggctg gtcttgagct cctgggctcaagggatcctt ccacctcggc 246420 ctcccaaagt gctgggatta caggcatgag ccaccatgcttggctgtcaa ttttttttga 246480 acatatcggt cataagtttc ttctcccaga tttttatttatcttaagttt cctgtgccgt 246540 ttctttatca ctccagtttt aggtgaattt tattcttatttcacaagtaa ataatgaaag 246600 cataggtgtg aactcctcaa aatctagttt tcttgtcaatctatcctatt ctttacctgt 246660 cttcattatc ttcactgtta tcttaaagga gggtaagctcctctcatctc atcttaactt 246720 cttttctctt tttcttttct atatcaacct ttttacctccattatcttct tactgtaaac 246780 atgaacaagt ctcccatctt aaaacagaga caaatccatgcagcaatctg tcatccttac 246840 taattactac tgttttattt caaataaatt aataaaatagtcaaacttca atggtgtcag 246900 attctttata gaaaaaaatt aaaaaatatc caaacttctaaaagtctttt cctcaccttc 246960 tgcttattct taacctacta cagtctggat gtgttggcatcttaaattat ttttgctcaa 247020 aagaaagctc ataatctcat ccatcaaacc tgctcttattctttattctc actaacctag 247080 ttaatggcca tatggtcttt atgagctaag gctttggaatcagacagatc aatgttgctc 247140 tgtaactatt ctaagttaga tgaaaaatgc atgtaaagtgcctgtgcata gaaagttttc 247200 agtagctatt atcttggcta ctgaacatca catcatcatcttgccaagag ttgttctact 247260 catcagtcat tctcacttag gttagaacaa ttttcctttttagtcaaatc gtattttggt 247320 tgtccttaaa atcctttagt ggcttttcat tgtttgcagggtacctttca aactccttgt 247380 atatatagaa tctttaagtt gacacctgcc tgtctttacaatcttactac cctacacttt 247440 gttgtgtgta ccctgtgctt cagccatatt gagcttcttttatttccttc acttaagtta 247500 attattgact gcctacagac aaggcacagc tgggaatatcgcagagaata aaactgacag 247560 gaagcccgga gattacattc agatagtgaa tatagacaaaaaaaaataat aatacataaa 247620 tgaaaataca tacagcttgt aattattaat gggaaggaaatacatattaa tatgattgtt 247680 ttgacttggc actgccctgg atgggggttc tgaggaaggctttctgagct agtaatattt 247740 gaggtaagaa ctgaaagtca aaatgaacca cctgttaaatgagctggaga aagtgcatta 247800 caggcagaag tgatagtaag ttcagtattc ttattctaagatttgtatta agccactagc 247860 acattttaag cttctgggat gtgtaattta tatttttaaaagatcattcc cgctgctctg 247920 tggggaatgc tttcaagagt gagaacagtg ataaaaagggagatcagtta gaaagctata 247980 cgctgtaaac actggtagta gtagagaggg aaagaagtcgaggtatagag gagaagagtg 248040 atgaattgga tagaaagagg ccgggtgcgg ggacccacacctgtaatgct agcactttgg 248100 gagggcaagg caggcagatt atttgaggtc aggagtttgagaccagcctg ggcaacatgg 248160 tgaaaccccc atctctactg aaaaatacaa aaattagctgcgtgtagtgg tgcgtacctg 248220 taatctcagc tactcaggag actgaggcag gagtattcttagaatccagg aggcggaagt 248280 tgcagtgaat cgagattgca ccactgtatt ccatcctgggcaacagagcg agacaccatc 248340 tcaaaaaaag aaaaaaaaaa gactgagtct aactggggttggagtggttt ggggataggc 248400 cagaagtaaa ggcatctatt gtggatatgt taactttaataagattatta gatatctagg 248460 tggagatgtt aaatagtcaa ttggtatcta agtctggagctcaggagaga gataccaaga 248520 gtcagaagaa catagtggat ggatttgtgt ttgattaaacagaagggtct taagaggtga 248580 cagttttatt ggtgcagcta agtttgtaag tttggggggaaagataagga atagtttatt 248640 tatatttatt taatagttta tattttgtca cctagagggtggtctccaag agtgggaaag 248700 cacactttaa ataaacttga atccttatgg ggcaacttactagttacatg aacatggaaa 248760 agttaacctc tcagaagctt agttatattt caaaaaaggagcctcagtgt agactaatat 248820 attccagtaa taattaaaag aggtatacta ctgtggaatcttattcatct gaattgtata 248880 aattggagtt cctaccatag ccttaagcct cttcaaccttttctggaaca atgcagggta 248940 taaattaata accacagcct tatttttctg cttttgcttgatgtatgttc attgttcttt 249000 gttaattcac ttcctttcta gctatactct tcaccttcttgtctgaaagt ggtaccgatt 249060 aatctctcat cactgggcaa gagctgggtt tagaagattttattactgta ttatatttta 249120 taactccttt tctttagaga tttctgctat ataaatagtttacataatgt gagaatttct 249180 ataattacta gctctattca agtcccagaa taaaaacaatagagacattt gaaaccagtt 249240 tacagcccat ataaagtgaa atgtgttaaa gtactctttctttaaatgta tgtttgtaac 249300 tttttgcttt cccagagatg gaaaaaaagc tgaaagaagaaagagaagct cgagagaagg 249360 ctgaaaatcg ggttgttcag attgagaaac agtgttccatgctagacgtt gatctgaagc 249420 aatctcagca gaaactagaa catttgactg gaaataaagaaaggatggag gatgaagtaa 249480 gaaaaatagt actgactttg ttcatctatt aaaagagaaacggttatata agttatgact 249540 ggagttatct ataatttaaa caccatgaag gagatttgaatgaaataatt gacatgggtc 249600 tgtgttcact gctgtatggt caccccagca cctagaattttcctcttcaa atagttattg 249660 aacattcact ctgtgtgatc tatgccattt ttcactatgacagtacaact atattgaaat 249720 gtcatagttc cttccctgaa aaaactgacc atctgctttaaagaaaaaaa aagtctttat 249780 tataatgcat cttatatccc caaagaaaag agcattgctcttagatttca ataaaagtgt 249840 ttaactgcag tctttcctgg cttagattga tagcacagggtatacatttt ataaagcagt 249900 taataaacag ataaaatgac agtatgtcct ggtggttactatatgtactt tagaaattat 249960 ttctaagact gaaagtagga aagccttttc aagaagatgtaacatgttac ctagatttta 250020 aaggttgagt aagacttcaa aaggtaatag ttttggagtcagggctttac ttgctagagg 250080 gaatattatc agtaaagaga tggataataa agtgagtaccggctggataa gaagatgcat 250140 aaggaaggca taaaaaggaa cttgatagaa ctttaactttttgaaggaaa aacgaagaca 250200 tgttgagtag tcatgggaga aatttgtagg ccaaagtatcgatactcttt aaaacttcca 250260 tgggtggcca ggcacgttgg ctgacacctg taatcccagcacttcgggag gctgaggtgg 250320 gcagatgggc acattgctta agctcaggag tttgaaaccagcctaggcaa catggtgaaa 250380 ccccatctca aatacaaaaa tcagctaggt gtggtgtcgcatgcctatag ttccagctac 250440 tcaggaggct gaggtgggag gattccttgc acctcaggaggtcgaggctg cagtgagcca 250500 agattgctcc atggcactca gcctggacat cagagtgagaccctatctct aaaaaataaa 250560 aaataaaact tgcatgggag ctataggtga aagcacttttagaaaaatca atctggtagt 250620 ggtatataga atggactaga aagaagagaa attagagatacctattaaag ctagcatgca 250680 ggaggaatgt atgttcaaaa tttgaaagat aatattcagtcatttaaaaa acataatttt 250740 tgactaccta tttggctctg tgctaggaat tgggggttaccgaagtgaat aagatatagg 250800 tatggtcctg actgtgttgg aacttacagc caggagggaaaaacagatat taaaatagta 250860 actacaagca ttgcaaataa ttagaaagaa agtacggaatgctatagggg tgtattacta 250920 atcacatttg ggagttacag aaggccttgc taagagtatgacatttgaga gctgaagaat 250980 gaataaatga aggaggataa aacaggagta gtgcgtacaagaaatgtgag gtgagggaga 251040 atttgttgca tttgaggatc tgaagaatat gattggattgtagagaatgg ccaatgatag 251100 gaatagaatt agaacagatc atgaaggcca ttgtaaaccactgtatgaat tttgagttca 251160 ttggtcccaa cacctaataa acacttcaga aatatttgttgagttgaata aaacatacgt 251220 tcagtttgtt tggttaagct ttgaagggtt gtagagtagagcacgtagac aggtaaattt 251280 aagtactcca ttcaaatgtt cccccaggaa tggcttttttttttttggag acagagtctc 251340 actgtcactc aggctggagt gcagtggcgt gatctcggctcactgcaacc tctgactcgt 251400 aggctcaagt gatcctccca tgtagctggg actacaggagtgtaccacca tgcctggcca 251460 atttttgtat ttttttgtag agacagggtt tcaccatgttgaccaggctg gtctcaaact 251520 ctgggttcaa gcaatccacc tgcctcagcc tcccaaagtgctgggattac agacataaac 251580 cactgtgcct ggcccagaag tgagttttat aatcaccttgtactacagaa ttttcatgct 251640 gattggggta gaaaggaacc agaatttttt tccctccacaagatctcatg tctgaaattt 251700 gggaattaat ttgacttaat agtaaatcta ctgtaataaacacgtggaac aatgaagact 251760 ctttaaaata ctaatatttt ctactagtac catgaattataatgtacttg atttggttag 251820 tagtctttca aataatgtct aataaattat ttaagagatttaagatccca attatttttc 251880 tcattcatag gttaagaatc taaccctgca actggagcaggaatcaaata agcggctgtt 251940 gttacaaaat gaattgaaga ctcaagcatt tgaggcagacaatttaaaag gtttagaaaa 252000 gcagatgaaa caggaaataa atactttatt ggaagcaaagagattattag aatttgagtt 252060 agctcagctt acgaagtaag tctttaaaat aacattgtgatttctttctt ttttgtgttc 252120 atcattgact tgtgtgtttg acattttaca tatgtacagattatgtactt agagtttgtg 252180 aaagacagaa aaaaagtatt tagaagtaaa agaatttacttgcaaaggtt ttcaggggag 252240 ttcaggcttt ggaaagacca cagttttctc atctgattttcatgtccaca aaagtcatca 252300 catttcttca ggttagaatt acaaaaatca cggtgccttgtaggagatgt tcaaggttta 252360 tttcagaggc tagaattaga catgacactt tgttcgttttgttttgtgtc tttggttcat 252420 ctcaaaatgc aaaaatgtct tttttctttt tctgttactcttgggatgtg atatgcctcc 252480 ttatttgaaa tggagaacaa gcaagatcag gaacgctttcacaatttgtc gaagaatatg 252540 cttcacaagt ttaatatcaa aagtggatat atcttatgccaagtcttgtg tttttatttc 252600 ctccacgttt attttatcat atcatcactt gctagcttcatttacaaaag tgattcttgg 252660 attaaattgt cagttttcat aaaattggat ttatattggtgaaattcttt tcctctttta 252720 gggccaccgt tttcgcaaaa gtgtgtttta tattttttttctccaaaaat actctcgctc 252780 agcattcagt ggagattggt gtagactttc tgtcagccacatgccttttg agatactgag 252840 aatacaaagc tattactgtc taatatgaaa gtctagccgctagattttca agcctagctg 252900 ctaggctttc atattataca gtaaataatt attgaaaatctgtgtctttg ggttacattg 252960 ggtttccatc ttttggactc tgctcctatg tgagttgtgtttatgtatgt attatataca 253020 tgcagttgtc tatctgcgtt cctccttgtt tgtcagtttgttgttcttgc tttcccttct 253080 tacctgatgg ttcatttcca ccagcccctt tcttgtgcttaattggccat aatatctagt 253140 atgcagtact gaattattac ttattaaata tagtactatgatatgtagca ctgaattatt 253200 attaaatttt tcatgccccc ctcttttttt atttactgtacaagacattt tcttccctat 253260 gactgctttt ctctgtaaaa atttaccaaa aatatggtatcaattttatt ttttcatttt 253320 ggatagatcc acaattaata ttctccaaaa agtgatcatcagtagaaaag ggttctcact 253380 acttttgcat agcgtcattt tataaggagc aagatagaaccggtgtggcc tgaccaagtt 253440 aattagtaaa ataatgaata atgaagtatg tttttaaaaaataaagattg ttttagtaaa 253500 cttttttata ttagcaactt taacaaacta agaaaaaatgaagtctagta taagtaggag 253560 cgtttgttca cttgtttttc ttttcatctg aagacagtatagaggaaatg aaggacagat 253620 gcgggagcta caagatcagc ttgaagctga gcaatatttctcggtaagtg agaatcattt 253680 ttaacataca cactgtttca aaacctgact taaaacaaggtatttagatt cttttatgtg 253740 aactgtaatt ctttttcaaa tgactcttca tcttctaataaacattgtga cttttctgct 253800 ttttgtttgt ccatttgttt cttggcatat taggctatcattggtatatg tttttaaatt 253860 gtaaaaactt ttcaggccag gtgcggtggc tcacacctgtcatcccagca ctttgggagg 253920 ccgaggcgag tggatcactt gaggtcagga gttcaagaccagcctggcca acatggtgaa 253980 accccgtctc tactacaaat acaaaaatta gccgattgtggtggcaggca cctgtagtcg 254040 cagctactca ggaggctgag gcaggagaat tgcttgagtccagtagttgg acattataat 254100 aagccaaggt catctgtgct gcagcctggg tgacagcaagactctgtctc ataaacaaaa 254160 tgaaagactt ttaaatattg ttgaaaactc tttccctgagtgtttgctaa acgtaattat 254220 tcaaagtatc tactgctaac ttctgcaaag tgatcggtagcatcccatat gctttcttgc 254280 catatgcttc cacagagcag tcatccagtg cctccaaatgtttggatggg tgtcaaatga 254340 cttgtggtga ttcttgtgga tttttgattt tgcttctagaaaatagtaca aaacctgacc 254400 ctatctcagt tctgatttat tccattgctt gtgggcattggattaaatag cattcaattc 254460 aataagctgt tatgaaccaa attttaagat ttgtcttttttctgggtttt caaatcagat 254520 tatttttctt tttatccctt cattgattta cttatttgaatttcagtatt tgaagtcaga 254580 gagataagaa tctgagtcct aaaggccaaa tagattaattttagaaatta taaatactta 254640 acctagagta agcccttatt cttttttcat ttgcattcccatggacattt atgtattttt 254700 ttgcctagga aagtgctgtt tcttagtgta ataaataagtgttaaaagta gttctccaca 254760 gctgtttcct ggcccttgct atttctttcc cttctctttttcttttaaaa tgaagagggt 254820 agttaaaaag aaatagacat cagaactgga agttcactctgtaacttcag caactaacac 254880 aatatgagtc tgatgtgtta aatatttttc actggcgttggcaaaagctt agtgaatttt 254940 gtaagagcca ttgttctgaa ataagcatat attatagatatgttagcaga atacttgata 255000 aatcttattt gtggatattt cttttttttt tgagacggagtttcaccctc ttgcccaggc 255060 tggagagcaa gtggcacgat cttggctcac tgcaagctctgcctcccggg ttcacgccat 255120 tctcctgcct cagcatcccg agtagctgga actacaggcgcccgccacca tgcctagcta 255180 attttttgta cttttagtag agacggggtt tcaccatgttaaccaggatg gtttcgatct 255240 cctgacccca tgatctgccc gcctcggcct cccaaagtgctggaattaca ggcgtgagcc 255300 actgcgcccg gcccttttgt ggatatttct ttacctgtctctcaactacc cagtacatcc 255360 cacccctcaa aacagaagtg atttttctaa cattagtcaagaattgatct aacctttaaa 255420 ttagtaatat gtgttaaatt tctttataac gtcttcccaaatctataata cactttatta 255480 agctataatg tctttttttt tttttttttt gagatggaatctcactctgt cgcccaggct 255540 ggagagtagt ggcatgatct tggctcactg caacctcccactcctgggtt caagcgattc 255600 tcctgcctca gcctcctgag tagctgggat tacagtagcatgccaccacg cccagctaat 255660 tttttgtatt tttagtagaa acgaggtttc actgtgttagccaggatgct ctcaatctcc 255720 tgaccttgtg atctgcccgc cttggcctcc caaagtgctgggattacagg cgtgagccac 255780 cgcacctggc cattaagcta taatttctat ataatatacccattttaagt gtactggtta 255840 gtaaattttg acaaatatgt acatctgtgt gaccaccaccacagtcaaca tgtggaacat 255900 ttttttttca ctgcaaaaag ttcccttatg gcccttcactctacattttc cccacccacc 255960 tctatacaca gacaatgact gtttttccat gattattgattattttcacc tgtcctagaa 256020 tttcatctac atggagtcac agtatacact ctttaacacgtggtttcttt cactcagcat 256080 attttttaga tttcttagat ttacatgttg tatgtatcagtagttcattc ctttttattg 256140 ctgagtaata tgaattaacc acagttttgt tttttgttttttttttaacc catgtattga 256200 tggatgtctg ggtcatttcc agtttggagc tgttataaataaaaccataa gggaaattca 256260 tgtgtaagac taggtgttga catatatttg catttgtcatgggtaaatac ttgagagtgg 256320 aattgttgga tcatatgtta attttttaaa tttataagaaactgccaaat tgtttttaac 256380 ccaccataaa cacttatttc tccttgtcac ttgttttgcagacactttat aaaacccagg 256440 taaaggaact taaagaagaa attgaagaaa aaaacagagaaaatttaaag aaaatacagg 256500 aactacaaaa tgaaaagtat gtccatttta ctttagaacaagaatcagta ctgcaacagg 256560 cattcttaag ttttnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 256620 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnntatccc 256680 aatatccacc tgcagattct acaaaaagat gtttcaaaactgctctgtaa aaagaattgt 256740 tcaactctgt tagttgagga catacatcac aaacaagtttctgagaatgc ttctgtctac 256800 tttttatggg aagatatttc ctttttcact gtaggcgtcaaagcgctcaa aatgtccaca 256860 tccagatact acaaaaagag tgtttcaaac cggctagatgaaagggaatg ttcaactctg 256920 tgacttgaat gcagacatca aaaagcagtt tctgagaatgcttctgtcta gattttatat 256980 gaagatattc ccgtttccaa cgaaatcttc gaagttatccaaatatccac ttgcaaattc 257040 tacaaaaaga gtgtttccaa actgctgtat caaaagaaaggtacagctct gttagttgag 257100 gacacacatc acaaataagt ttctgagaat gcttctgtctactttttatg gaaagatatt 257160 tcctttttca acataggcca gaaagcgctc aaaatgtccacttccagata ctacagaaag 257220 agtgtttcaa acctgcttta tgaaagggaa tgttcaaacctgtgacttaa aagcaaacat 257280 cacaaagaag cttctgagaa tgttgctgtc tactttttatatataatccc gtttccaacg 257340 aaatcctcaa agccatccaa atatccactt gcagattccacaaaaagagc gtttcaaaac 257400 tgatctataa atagaaaggt tcaactctgt tagttgagtacatatatccc aaagaagttt 257460 cttagaatgc ttctgtctag tttttatggg aagacatttcctttttcacc aaaggcgtca 257520 aagcgctcca aatgtccact tccagatact acaaaaagagtgtttcaaac ctgctctaag 257580 aaagggaacg ttcaactctg agacttgaat gcagatatcacaaagtcgtt tccgagagtg 257640 cttctgtcta gattttatat gaagatattc ctgtttccaacgatatcttt aaagcaatca 257700 aaatatcaaa ttgcagattc tacaaaaata gtgtatcaaagctgctctgt aaaaagaaag 257760 gttccactct cttagctgag tacatacatc acaaacatgtttctgagaat ccttctgtct 257820 agtttttatg ggaagatatt tccattttca ccataggcgtcagagcgctc caaatgtcca 257880 cttcccgata ctacaagaag agtgtttcaa actggctctatgaaagggaa tgttcaactc 257940 tacgagttga atgaaaagat cacaaagaag tttctgagaatgcttctgtc tagatttcat 258000 atgaatatat tcccatttcc caggaaatcc tctaagctatccaaatatcc acttgcagat 258060 cctacaaaaa gagtgtttca aaactgctct gtcaaaagaaaggttcaact ctgttagttg 258120 aggacacaca tcacaaagaa gtttctgaga atgcttctgtgtaggtttta catgaagata 258180 ttgccatttc caacgaaatc cttaaagcca ttcaaatatgcacttgcaga ttctacaaaa 258240 agagtgtttc aaaacggctc tgtaaaaaga aaggttcaactctgttagtt gagtacacaa 258300 atcacaaaca agtttctgag aatgcttctg tctcgtttttatgggaagat atttcctttt 258360 tcaccaaaat cattaaaggg ctccaaaggt ccacttccagatactacaaa aagagtgttt 258420 caaacctgct ccatgaaagg gaatgttcaa ctctgtgacttgaatgccga tatcacaaag 258480 tagtttctga gagtgcttct gtctagattt tttatgaagatattcccgtt tccaacgaaa 258540 tcgttagagc tatccaaata tccacttgca gattctacaaaaagagtgtt tccaaactgc 258600 tgtatcaaaa gacaggatgt actccgttgg ttgaggacacacatcacaaa gaagtttctg 258660 agaatgcctc tgtctagatt ttatatgaag atattcccgtttccaatgaa atccttaaag 258720 ctctccaaat atccacttgc agattctcca aaaagagtctttcaaaagtg ctctgtaaat 258780 agaaagtttc aactctgtta gttgaggaca aacatcacaatccagtttgt gagaatgctt 258840 ctgtctagtt tttatgggaa gatatttcct tttgcaccgtaagcgtcaaa gcgctccaag 258900 tgtccacatc cagatactac ggaaagaggg tttcaaacctgctctatgaa agggaatgat 258960 caactctgtg acgtgaatgc agacatcata aagcagtttctgagaatgtt tctgtctagg 259020 ttttctatgt tgatactccc gtttccaacg aaatagtgagagctatccaa ctatccactt 259080 acatattcta caaaaagagt gtttccaaac tgctgtatcataagaaagga tgaactctgt 259140 tagttgagga cacacatcac aaagaaattt ctgagaatgcttctgtctag tttttatggg 259200 aagatatttc cgttttcaac ataggcctga aatcgctcgaaatgtccatg tccacatact 259260 acagaaatag tgtttcaaac ctgctctatg aaagggaatgatcaactctg tgacgtgaat 259320 gcagacatca taaagcagtt tctgagaatt tttctgtctaggttttctat gttgatactc 259380 ccgtttccaa tgaaataatg agagctatcc aactatccacttacatattc tacaaaaaga 259440 gtgtttcaaa actgctgtat cataagaaag gatgaactctgttagttgag gacacacatc 259500 acaaagaaat ttctgagaat gcttctgtct agtatttatgggaagatatt tccgttttca 259560 acataggcct gaaatcgctc gaaatgtcca tgtccagatactacagaaat agtgtttcaa 259620 acctgctcta tgtaagggaa tattcaactc tgtgacttaaaagcaaacat cacaaagaag 259680 gttctgagaa tgctgctgtc tactttttat atgtaatcccgtttccaagg aaatcctcag 259740 agctatccta atatccactt gcagattcca caacaagagcttctcaaaac tgacctataa 259800 atagaaaggt tcaactctgt tagttgagta catatatcccaaagaagttt cttagaatgc 259860 ttctgtctag tttttatggg aaaacatttc ctttttcaccaaaggcgtca aagcgctaaa 259920 aatgtccact tccagatact acaaagtgtc tcaaacctgctctaagaaag ggaatgttca 259980 actctgtgac ttgagtgcag atatcacaaa gcagtttctgagagtgcctc tgtctagatt 260040 ttatatgaag gtattcccat ttcctatgaa atcgttagagctatccaaat atccacttgc 260100 atattctaca aaaggagtgt ttcgaaactg ctgtatcaaaagaaaggttg aactctgtta 260160 gttaaggaca cacatcacaa agaagtttct gagaatgcttctgtgtagat tttacatgaa 260220 tatagtcccg tttccaagga aatccttaaa gccatccaattatccacttg cagattttac 260280 aaaaagagtg tttcaaaact gctgtgtaaa aagaaacgttcaactctgtc agttgagtac 260340 acacatcaca aacaagtttc tgagaatgct tctgtctagtttttatggga agatatttcc 260400 tttttctcca aagtcggcaa agcgctccaa agatccatttccagatacaa caaaaagagt 260460 gtttcaaacc agctctatga aagggaatgt tcaactctgtgacttgaatg cagatatcac 260520 aaagtacctc ctgagagtgc ttctgtctag attttatatgaagatattcc cgtttccaag 260580 gaaatcgtca gagctatcca aatatccact tgcagattctgcaaaaagag tgtttccaaa 260640 ctgctgtatc caaagacagg ttgtactctg ttaggtggggacacatatca caaagaagtt 260700 tctgagaatg acttttccgt ttccaatgaa atctacttaaaggtatccaa atatccgctt 260760 gcagattcta caaaaagagt ctttcaaaac tgccctgtaaatagaaaggt tcaactctgt 260820 tagtggagga catacatcac aaagcagttt gtgagaaggcttctgtctag tttttaaggg 260880 aagatatttc ctatgtcacc gtacgcgtca aagcgctcaaatgtccacat ccagatacta 260940 cagaaacggt gttacaaacg tgctcgatga aagagaatgttcaattctgt gacttgaatg 261000 cagacatcac aaagcagttt ctgagagtgc ctctgtctagattgtatatg aagatattcc 261060 cgtttccaac gaaatcttca aagttatcga aatatccgcttgcagatact acaaaaagag 261120 tgtttctaaa ctgttgtatc aaaagaaacg ttcaactctgttagttgagg acacccatca 261180 caaataagtt tctgagaatg cttctgtcta ctttttatgggaagttattt cctttttcaa 261240 cataggcctg acatcgctcg aaatgtccac ttagagatactacagaaaga gtgtttcaaa 261300 actgctctat ggaagggaat gttcaactct gtgacttaaaagcaaacatc acaaggagct 261360 tctgagaatg ctgctgtcta tattttatat gtaatcccgattccaacgaa atcctcagag 261420 ctatccaaat atccacttgc agattccaca aaaagagcatttcaaactga tctataaaga 261480 gaaaggttca actcggttag ttgagtgcat atatcacaaagaagtttctt agaatgcttc 261540 tgtctagttt ttatgggaag acatttcctt tttcaccaaaggcgtgaaag cgctccaaat 261600 gtccacttcc agatactaca aaaagtgtgt ttcaaacgtgctctaagaaa gggaatgttc 261660 aactctgtga cctgaatgca gatatcacaa agtagctcctgagagtgctt ctgtacagat 261720 tttatatgaa gacattcccg tttccaacga aatcgttagagctatccaaa tatccacttg 261780 aagattctac aaaaagagtg tttccaaact gctgtatcaaaagaaaggtt gaactctgtt 261840 agttgaggac acacatcaca aagaagattc tgagaatgcttctgtgtaga ttttacatga 261900 atatagtccc gtttccaacg aaatccttaa agtcatccaattatccactt gcagattcta 261960 caaaaagagt gtttcaaaac tgctgtgtaa aaagaaacgttcaactctgt cagatgagta 262020 cacacaacac aaacaagttt ctgagaatgc tttggtgtagtttttatggg gacatatttc 262080 ctttttcacc aaagtcgtca aagagctcca aagatccacttccagataca aaaaaagagt 262140 gtttcaaacc agatctacga gagggaatgt tcaactctgtgacttgaatg cagatctcac 262200 aaaattagtt tctgagagtg cctctgccta gattttatatgaagctattc ccgttttcaa 262260 caaaatcgtt agagctatcg aaatatccac ttccagattctacaaaaaga gtgtttccaa 262320 actgatgtat caaaagacag gttgtactct gttagttgaggacgcacatc acgaagaagt 262380 ttctgagaat gcctctgtct agattttacc tgaagatattccggtttcca atgaaatcct 262440 taaagatctc cagatatcca cttgcagatt ctccaaaaagagtctttgaa aactgctctg 262500 taaatagaaa ggtttaactc tgttagctga ggacatacatcacacaccag tttgtgagaa 262560 tgcttctgtc tagttttcat gggaagttat ttccttttcaccgtaagcgt caaagcgctc 262620 caagtgtcca catccagata cttcggaaag agtgtttcaaacctgctcta tgaaagggaa 262680 tgttcaaatc tgtgacgtga atgcagacat tacaaaggagtttctgagaa tgtttccgtc 262740 taggttttat atgaagatac tcccgtttcc aaagaaatccacaaagctat ccaaatatcc 262800 aattgcagat tctacaaaaa gcgtgtttcc aagctgctctgtcaaacgaa atgttctatt 262860 ctgtgagttg aggacacgca tcacaaacaa gtttctgcaaatgcttgtgt ctggttttta 262920 tgggaagata tttccttgtt caccataggc ctgaaaacactcgaaatgtc cacttccaga 262980 tactacagaa agagtgtctg aaacctgctc tacgaaagggaatgttcaac tctgtgactt 263040 aaaagcaagc atcacaaaga agcttctgag tatgctactgtctagttttt atgggaagat 263100 atttcgtttt tcaacatagg cctgaaatcg ctcgaaatgtccacttccag atactataga 263160 aagagtgttt ctaaactgct ctatggaagg gaatgttcaactctgtgact taaaagcaaa 263220 catcacaaag aagtttctga gaatgctgct gtctacattttgtttgtaat cccgtttcca 263280 acgaaatcct gaaagctatc caaatatcca cttgcagattccacaaaaag agcattaaaa 263340 agctgatcta taaagagcaa ggttcaactc ggttagttgagtacatatat cacaaagatg 263400 tttcttagaa tgcttctttc tagtttttat gtgaagacatttcctttatc accaaaggcg 263460 tcaaagcgct ccaaatgtcc acttccagat cctgcaaaaagagtgtttca aacctgctct 263520 aagaaaggga atgttgaact gtgtgacttg aatgcagatatcacaaagca gtttctgaga 263580 gtgcctctgt ctagatttta tatgaaggta ttcccgtttccaaagaaatc attagagcta 263640 tgcaaatatc cactggcaga ttctacaaaa agagtgtttccaaactgctg tatcaaaaga 263700 caggatgtac cgccgttagt tgaggacaca catcacaaagaagtttctga gaatgcctct 263760 gtctagattt tatatgaaga tattcccgtt tccaatgaattcnnnnnnnn nnnnnnnnnn 263820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 263880 nnnnnnnnnn nnnnnnnnnn nnatcctaac ctttacccctatgcctccaa atctacaaat 263940 cctacttctt ttacttccta cttcgtttta tgcacttgcctcctactgcc aacttccact 264000 ataattttac atctaagttt tgccagcggt tatataattgggctccctgc atccacccag 264060 ctttccctta cttcaattca ttctccactc cttagagtgagttttctaaa atacttatca 264120 gtcactagct taggattttc atttattcat tcaatatttattgaatactt attctctgaa 264180 agtccctgat tggagtgctg ggaatagaca atagacaacaatagaagaaa caaaagtccc 264240 cacttttcaa tattttaaac tcttcagcat gatacacaaggtcctttgta atctgactca 264300 gttttctctc tccagcctta tctgtcacga actaaccatttgctgttcct cagatcacag 264360 attggcttct gcctatattg tcccttgttc cccacatcccattccctggc taattctact 264420 ttccatcggt tgtgatctct gttaggaaat gttctctgaatcccacatgg taagatgagg 264480 tatcctttgg catagccact ataccttgat tatatctctcctaacttgat tctgaagcac 264540 ctgaagaatc ataacttact aataattgga tagctgcacaaagcacagta cttatagcac 264600 aatactggtg atccatatat ttgtcttgaa tagatgaaaaagggaaaata tatcaactct 264660 gaaacaatat agtatggatt gaatatttgg cacttttaatcactttctct ttctgttcct 264720 cagatcactg attggcttct gcctaaactg tccccttcacagtgaatgca tacacctggg 264780 attgcatatt gcactgtgaa taccacacaa atggtaggtaacagactaga ttctacaata 264840 tgactatatc agagaataac caggaaatgc agctttgtgtctaccttagc ttccagttaa 264900 ttaggttatc taactatgat acttggccac cgaattagtatgtttatacc cgctgtgatg 264960 ctaaaattta agataatata cagctctgca acaaactattgtcaattttg gaccttaaat 265020 attttaatca atataatcaa ctcgattaag gcaacttaattgcaaaaaga tgcaattata 265080 cttaattgta atcaagtcaa caaagttaaa caacaaaaagtctaagttta tttataaaac 265140 tagtgaaaat aaaagttatg tattccaaca aaaaaaaaaaaaaatgaaga atgataaaag 265200 tgagtatctc cagtttgcaa cacctaatgt attaaggatctaacattgaa tgccatagtg 265260 gttaacaaca gaaataggca accagaaagt acaaggctatatatgaatta gtcttcttcc 265320 tcctaaaaat cagatttgaa tctgattaag cttctaaatcagaaatgtcc aataaaactt 265380 tccaggatgt cagaaatgtt ctatatctgt accatctattataatatggt agacactaac 265440 tgcatgtgac tactgagcac ctgaaatacg actagtacatctgaggaact aagttttaat 265500 tttacgtaat tgaatttaaa tttaagtaac tacatgtggctactggccac catattgggc 265560 agaggagctc tagatacaac tactgacttc caggaaacataagggaaatg gggaatgttc 265620 aaccaactac agaaatgcaa tcaataaaat cgcaactgagggaacatcta caggacaaaa 265680 aaatccagtt tcttcaacaa attagtttta ggatggtgattactcttgag gtggggagga 265740 aagaaggcat aattaggagg tgggatatag tgggcttctgaagtggatag caaggttctc 265800 tttcatggat gatgatggtt ataatgacat ttgtcatagttcataagaac tgtattagtt 265860 ttgctgtttt ctgtaattgt ttgtttcaac aacagtctttcaaaggtgat taaatgtaaa 265920 gagaatataa caatgttgga aaactagttc tagtgtctgttctgacatta cctagctatg 265980 agatactccg caggagttac tatcttgttg cgcctgtcagctgaattcta aaatgaagct 266040 gttacacttg aaatagcttt ataaggtttt ctccttggagatggggtctc attatgttgc 266100 ccaggctggt cttgaactct tgggctcaag tgatcctctcaccttagcct cccaaaatgc 266160 tggtattaca ggcatgagcc accatgcccg gccataacctgcatagtaat cctaagaaga 266220 ccttaaatac ctttatctaa cttaaaataa tgatttgcaaagacagcatg atttatatat 266280 aaatagatct tttaaaattg atactagccc aggcgtggtggctaatgcct ataatcccag 266340 cactttggga ggccaaggtg gacggaatat gaggtcaggagtttgagacc aacctgacca 266400 acatggtgaa accctgtctc tactaaaaat acaaaaattagcggagcgtg gtggcgcacg 266460 cctgtaatcc cagctactca ggaggctgag gcaggagaatcgcttgaacc cgggaggtgg 266520 aagttgcagt gagccgagat catgcnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 266580 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 266640 nnnnnctgga attataggca cctgccacca tacccggctaattttcgtat tttttaatac 266700 agacggggtt ttgtcatgtt ggccaggctg gtcttgaactcctgatctca ggtgatctgc 266760 ccgcttcagc ctcccaaggt gctgggctta caggcaagagccactgtgcc cagccttaaa 266820 tgcatttttg ctaagtgaaa aaaagacaga atcaaaaggctatacactgt atgattccat 266880 ttaatgacat tctggaaaag gtaaaactat aggaacagaaaaagagatca gtgtttccca 266940 ggagtggggg tgatggaact gttccgaatg gtattggggtgttgcagaca agactatgtg 267000 tttgttaaga cccacagaac tatacacact ttatgcaaatttaaaaaatc aattgggatt 267060 ctgggggatc tcaggatgga atccagattc taacaaatgaatctaaatgt atgactaaca 267120 agatgacatg ccctcattga aggtggtgag ggaaaagaagctgaactacg taatgctgga 267180 aaactgtttt agctggaaac taaggctaaa gacaaaaaaggaactgtaca aagatattgt 267240 gttccagttg gtaaatccat ttctcacagg gtgtatggtttattttacat gaatattagg 267300 gttgagtaag taaataccaa taatgggagc caggtttctcatcatcagag aaaaaaagtt 267360 acaaacaggc aagggcaaaa gctagaatga atcttgtgatactagtttag agctggtaat 267420 atcagtctaa acacgtttaa catgtgtaca ggtatagaattatagttatg tgtatagtca 267480 caggctggta tacatgcata catatattgt cctagctatggcagctgaga gggccttgaa 267540 acagtgatca ccagtagcaa taagcacacc tagcacccagatcttggttt ctaaatatca 267600 ttctccaata aaaggaacca gagctccttg gaaaaaatggctgatttcag ggctgaggga 267660 aaatataaga tgagcctgga gcatcgtgta atgccagaaagtaaagaagt gttccttttt 267720 ttaaaaaaag taagaacatg tcaaagaaca caggagtaggagctgggtcc aacctgaagg 267780 agctcccaaa aggcaaaaat gagaacaatt taataaaattnnnnnnnnnn nnnnnnnnnn 267840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 267900 nnnnnnnnnn nnnnnnnnnn tcaacttgca gattctacaaaaatagtgta tcaaagctgc 267960 tctgtaaaaa gaaaggttcc actctgttag ctgagtacacacatcacaaa catgtttctg 268020 agaatccttc tgtgtagttt ttataggaag atatttcctttttcagcata ggcatcagag 268080 cgctccaaat gtccacttcc cggtagtaca agaagagtgtttcaaaccgg ctctatgaaa 268140 gggaatgttc aactctacga gttgaatgaa aagatcacaaagaagtttct gagaatgctt 268200 ctgtctagat tttatatgta tatattcccg tttccaacgaaatcctctaa gctatccaaa 268260 tatccacttg cagattccgc aaaaagagtg tttcaaaactgctctgtcaa aagaaaggtt 268320 caattctgtt agttgagtac aaacatcaca aagaagtttctgagaatgct tctgtctagt 268380 ttttatggga agatatttcc tttttcacca tgggcctcaaagtgcttcaa acgtccactt 268440 ccagatacta caaaaagagt gtttcaaacc tgctctatgaaagagaatgt tcaattctgt 268500 gacttgaagg cagacatcac aaagcagttt ctgagaatgcttctgtcgag attatatacg 268560 aagatactcc cgtttccaac gaaatcctaa aatctatccaaatatccact tgcagattct 268620 acaaaaagag tgtttcaaaa ctgctctgtg aaaagaaagcttcaactctg ttagctgagt 268680 acacacatca cagacaagtt tctgagaatg cttctgtctattttttatgg gaagatattt 268740 cctttttcac cataggcgtc aaagcgctcg aaatgtccacttccacatac tacaaaaaga 268800 gtgtttcaaa ccttctctat gaaagggaat gttcaactctatgagttgaa tgcaaacatc 268860 agaacgaaat ttctgagaat gctgctgtcc actttttatatgaatgcgcg ctttcaacga 268920 aatcctcaaa gcaatccaaa tatccacttg cagattccacaaaaagagtg tttcaaaact 268980 cctctatcaa tagaaagttt caactctgtt agttgaggacacacatcaca aataagtttc 269040 taagaatgct tctgtgtaga ttttacatga agatattcccgtttccaatg ccatccttaa 269100 agccatgcaa atatccactt gcagattcta caaaaagagtgtttcaaaac tgctctgtaa 269160 aaagaaaggt tcaactctgt tagttgagta cacacatcacaaacaagttt ctgagaatgc 269220 ttctgtctcg tttttatggg aagatatttc ctttttcaccaaagtcgtca aagcgctcca 269280 aatgtccact tccagatact acaaaaagaa tgtttcaaacctgctccatg aaagggaatg 269340 ttcaactctg tgacttgaat gccgatatca caaagtagtttctgagagtg cttctgtcta 269400 gattttatat gaagatattc tcatttccaa tgaaatcgttagagctatcc aaatatccac 269460 ttgcagattc tacaaaaaga gtgtttccaa actgctgtatcaaaagaaag gttgaacttt 269520 gttagttgag gacacacatt acaaagaagt ttctgagaatgcttctgtct agattttata 269580 tgaagatttt cccgtttcca atgaaatcct taaagctatcccaatatcca cctgtagatt 269640 ctacaaaaag agtgtttcaa aactgctctg taaaaagaatggttcaactc tgttagttga 269700 ggacacacat cacaaacaag tttctgagaa tgcttctgtctagtttttgt gggaagatat 269760 ttcctttttc actgtaggca tcaaagcgct ccaaatgtccacattcagat actacaaaaa 269820 gagtgtttca aacctgctcg atgaaaggga atgttcaactctgtgacttg aatgcagaca 269880 tcacaaagca gtttctgaga atgtttctgt ctagattttatatgaagata ctcccgtttc 269940 caatgaaatc cacaaagcta tccaaatatc cacttgcagattctacagaa agcgtgtttc 270000 caaactgctc tgtcaaacga aatgttcaac tccgttagttgaggacacac atcagaaaca 270060 agtttctgcg aatgcttctg tgtagttttt atgggaagatatttcctttt ccaccatagg 270120 cctgaaagcg ctcgaaatgt ccacttccag atactacagaaagagtgttt caaacctgct 270180 ctatgaaagg gaatgtttta tatttgtatg tatcatatgccaattgttac cttttttggc 270240 agcactgaaa attcaggtga tcttaaacat cagacagataacacaaataa gtggtccagg 270300 taaggactgt gtgaaataga aaacatatcc tctctcagaaaacagtgaga ggatattgct 270360 gatgtgcagg cctctggccc tttgttgaga aacagttattggaaatcaga tttgtatttg 270420 aagtattcca gtttttaaat tagcaactat tttcttgcaaccccagcact tttggaggcc 270480 aagatgggca gattgcttga gctcagtagt tcaagaccagcctgggcaac atggcaagac 270540 cccatctcta ccaaaaatac aaaaaattag ctgggcatggtggcatgtgc ttgtggtccc 270600 agctactagg gagactgagg tgggaggatc acttgagcccaggaggcaga gcttgcaatg 270660 aaccgagatt gcaccactgc actccagcct gggcaagagagtgagacccc atctgatgtg 270720 atttggctgt gtccccaccc aaatctggta ttgaattcccacatgtgtgg gagggacctg 270780 gtgggaggta gttgaatcat gggggcaggt ctttcctgtgctgttctcat ggtggtgaat 270840 gactctcatg ggatctgatg attatagaag ggggagtttccctgcacaag ctcttttatc 270900 ttgtctgcca ccatgtgaga catgcctttc accctccaccacgattggga agcctcctca 270960 gccacgtgga actgtaagtc cattaaatgt ctttcttttgtaaattgccc agtctcaggt 271020 atgtctttat cagcagtgtg aaaatggact aatacagtaaattggtacca gaagtggggt 271080 gttgctgaga agataccgga aaatgtggaa gcaactttggaactgggtaa caggcagagg 271140 ttgaaaccgt ttggagggct cagcagaaga caggaaaatgtgggaaagtt tggaacttcc 271200 tagagatttg ttgaatggct ttgtccaaaa tgctgatagcgatatggaca ataaggtcca 271260 ggctgaggtg gactcagatg gagatgagga acttgttgggaacaggagca aaggtgactc 271320 ttgttatgtt ttagcaaaga gactggcaac atttcgcccctgccttagag atttatgtaa 271380 gtttgaactt cagagagatg atttagggta tctggtggaagaaatgtcta agcagcaaag 271440 cattcaaggg gtgacatggg tgctgttaaa ggcattcagttttataaggg aagtagagca 271500 taaaagttca gaaaatttgc aacctgacaa tgtgatagaaaagaaaaact cattttctga 271560 ggagaaattg aagctggctg cagaaatttg cataagtaagatgttaatcc ccaagacaat 271620 gggaaaaatg tctccagggt atgtcagagg ccttcacggcagcccctccc atcacagacc 271680 cagaggccta ggataaaatg gttttgtggg ccaggcagggtccacatgct gtgtgcaatc 271740 taaagacttg gtgctctgca tcccagccac tccagccgtcactaaaaggg gctaagagac 271800 agcttgggct gttgcttcag agagtggaaa acccaagccttggcaggttc catgtggtgt 271860 tgagcctgta ggtgcagaga agtcaagaac tgaggtttaggaaccttggc ctagatttca 271920 gatgtatgga aatgcctgga tgcccaggca gaagtttgctgcaggggtgg ggccctcatg 271980 gagaaccttt gccagggcag tgcagaaggg atgtgaggttggagtctcca cacagagtcc 272040 ttactggggt accgcctagg gccaccatcc tccagaccccagaatggtag atccactgac 272100 agcttgcact gtgtgcctgg aaaagccaca gacactcaacaccagcccat gaaagcagcc 272160 aggaaggggg ctgaaccctg cagtcacagg ggcagggctgcccaagacca tgggaaccca 272220 cttctcccaa cagtgtgacc tggatatgag acatggagtcaaaagatgtc actttggagc 272280 tttaagattt gactgcccca ctgtattttg gacttgcatggatcctgtag cccctttgtt 272340 ttggccaatg cctcccattt ggaatggctg tatttatccaatgcctgtgc ccccattgta 272400 tccaggaagt aactaacttg cttttgattt tacaggctcataggcggaag ggacttgcct 272460 ggtctcggat gggactttgg actgtggact tttgagttaatgctgaaatg agttaagact 272520 ttggaagact gttgggaagg catgattggt ttgttttgtttttccttttc tttttttaag 272580 atggagtctt gctctgagtc ccaggctgga atgcagaggcatgatctcaa ctcactgcaa 272640 cctctgcttc ccaggtttaa gggattctcc tgcctcagcctcctgagtag ctgggattat 272700 gggtgcctgc caccatgcct ggctaacctt tatatatttagtagaaacag ggtttcacca 272760 tgtcaggcta gtcttgaact cttgacctca ggtgattcacccacctcagc ctcccaaagt 272820 gctgggatca caggcatgag ccactgcacc cggctgcatgattggttttg acatacgaag 272880 acataagatt tgggaggggc cagggatgga atgatgtagtttggctgtgt ccctacccaa 272940 atctcatctt gaattcccag gcattgtggg aggctttgggggaggtagtt taatcatggg 273000 ggcacgtctt tcctgtgctg ttcttgtggg ggtaagtgaccctcacggga tctgatgatt 273060 atataagtgg aagctttcct gcacaagctt tcttatcttgtctgcctcca tgtgagacaa 273120 gcctttcagc ctccaccatg attgtgaagc ttccccagccacatggaact gtaagtccat 273180 taaatgtctt tcttttgtaa attgctcagt ctcaagtatgtctttattaa cagggtgaaa 273240 atggactaat acacatctca aaaaaaagat aaattagcaactaaacgata tttttaaaat 273300 atgatatgag tctaacaaaa caaataaggc taaatatgactaaggtctct tgctagttct 273360 aatcattttt cagttagttg tttggaacag ttttggtaagcaatcatgat cggcaagtaa 273420 tggcaggttt gctgcttctt tccaatatgt gtaatacgtatttgtgtttt gctgcattta 273480 tctcagaaaa atgttgaata atagtaggaa tcctggttgtttctgactct ggtttccttg 273540 ttaattacaa tgtttgttct aggttggcct gcctcttttatagttagagg agttattgat 273600 cacataattt gtaataataa tatgattaac atgagaatagttgagagttt ctactttttg 273660 agtatttgtg gtgcaagtgt gaattaatgt aatacattcattaaacaagt gagctaatga 273720 gaaatacaat ttcaggcacg attatgaaga tgttcccttttttctatgac agtaaatgca 273780 ctttcttgaa agcctggttg gaatagatat gacattataatgtaatgtaa atactctttg 273840 gattgtaaat ggtataaata atgttgatga aataaacaaggtatagaggc agggcttcgt 273900 ggcttacata tgtaatccca gcactttggg aggccaaggtgggaggattg cttgggccta 273960 ggagttcaag agcagcctgg acaacagtgg aaccccatctctacaaaaag taagaaaatc 274020 agccgggcat ggtggtgcat gcctgtagtc ctagctacttgggaggctta ggtgggggga 274080 ggatcgcttg agcccaggag gtcgaggctg cagtgagctgtgatcacacc attgcactcc 274140 agcctgggcg acagagcaag accttatctt aaacaaaaacaaaaaaacaa aaacaatcaa 274200 acaaaaacaa aaaaagccaa gttatagagt attacatgtgaaaagtgtaa tgccatttat 274260 ataaatgtag aagccatata aaacagcttt atatagatacaagggtttta gcaaagggac 274320 agaatgcgaa tgaggagaat acacatcaaa ttaacagcagttatttatgg cttttgaggg 274380 ggaaggaaag agaatagaac tgggaacgga ggtacacagaagtctttggt taaatctgta 274440 atttttattt ctttaaaaaa agcaatttaa aggaaatataaagacaatat ttgctcattc 274500 tgggtagtag gtataaaaat gcattttata ttttttgcagaattaaaaaa tatcagtatt 274560 atcttgaact tagtaaaata aatgagccta ttcacagatagcagtgttta atgcagtata 274620 attatatgga taattagaat aacaatatgg tacacaggacagacctagga attcttgttg 274680 aatagtcaga accacagaag tgagctgtag ttgacacaggatgtaatgta tatatggtgt 274740 aaaaaggaaa aataagaaaa atttctataa tagctaatattagtttctga atattatgaa 274800 atgacaacat acaacatata tccaactgaa caatgcctagagatgaattt tagtaataaa 274860 gttcctgtta aatgacatta ccataaagga aaaataatctatagtgttca taaagctcat 274920 aattcattta atgtttcctt tatgaagtgt agcctgctagaggctttcag tccagtaaaa 274980 aatgacacct attattatat ttgttatttt gcatttgataaaatgttcat ttacagctat 275040 atttctgcct actataaatg agtgatgagt tcatactataaagtttaaaa aaccaagact 275100 tatttccata ccatgacagt gaaatcaaag ttttcccctctaaataacag attatgatat 275160 atgaaatata ctaaagccca gtaagtcagg attcaaattcatgactgttt ttttttcagc 275220 tgtaagttag tttaccttca cattccatct agcaaattgaggtttatagc tgtgtgccct 275280 agaacagaat attatgtatt ttattattta ttgagacagagtctgtcacc cgggctggag 275340 tgcagtggca caatcacggc tcactgcagc ctcaacttcctgggctccag caatcctccc 275400 acctcagcct cccaagtagc tgagaccaca gatgcatgccaccacacctt gctacttttt 275460 tgtattttgg tagaaacagg gttttgctat gttgcccacggctggtcttg agctacctaa 275520 gtgaccactg gccttggcct cccaaagtgc tgagataacaggcatgagcc actgtgcctg 275580 gcctaaacat ataaaattgt cctatcataa agtagggatgttaataccta tagttggtaa 275640 taccttgtaa agttgagaat aggatacttt ttgtaaaggtgaaagttacg ttttaacctt 275700 gaaacatctt aatttcaaga tcttgaggta ataacgtagtggaaacattt ttttttcttt 275760 tctagttttg acctccttta tgcctactct tagatggcccctcttgtgat taattgacat 275820 attttgaatg agaattaggc ctgagcagag ttagcaaatgtgttgcacac agcacagaac 275880 ttacatcaac cagaactaat gaaaccttgg ccaagagtgagtaaatctaa gaaacatcca 275940 ttcaggctgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 276000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnna tttttcaaga 276060 gaaaccaaga atctgaattt ttatgtaaaa ggttgacccaaaactctttt aggtgtatgt 276120 ttgtgtatgt gtgtctggat ccctccagac aattttctaggcatttgaaa tatacctgtg 276180 tgaacaagaa caaagccact ctttataatc ttataatccagaagaatatg gaattattga 276240 ttattgaatt cttttaagag gtaggagaat aagggtgagagattgacctt aaatattatt 276300 aaagacagtc tttcattatg gagagttgta aaggaagtctggatgaatat atagatgcag 276360 ggggcactgt tgtaggctca tttggtcctc tcaggtagcaaattgtaatc catagtgtag 276420 gatcagaaga cacaaaggct gaggaaagac tactagctcctagactttta aggaatgggc 276480 agaggaaggg aaaccaataa gctaccaaca gtgtaagagaagatcagagc aggatatcac 276540 aaaagctggg gtaagagaat atttccagaa agagaaagtgattaaaagtg ccacatattg 276600 gccgggcatg gtggctcacg cctgtaatcc cagcactttgggaggccgag gcaggcggat 276660 cacaatatca ggagattgag accatcctgg ctaacagggtgaaaccccgt ctctactgaa 276720 aatacaaaaa aattagccgg gcgtggtggc aggtgcctgtagtcccagct actcgggagg 276780 ctgaggcagg agaatggcgt gaacctggga ggcggagcttgcagtgagcc tagatggtgc 276840 cacggcactc catcctgggc aacagagtga gactgtctcaaaaaaagaat aaataaataa 276900 aaattaaatt aaattttaaa aagtgccaca tacccacaaaggtcaagaaa aacagtcata 276960 aattgtccat ttatttatat tgaaggttat tcattatcttggtgagaaca gcttctaaat 277020 agagatgaga ataggtatta aagtagattg agactgaatggaaagggtaa aacaagagac 277080 agagtatatc taacagcctg attagcctta tttctattcaacaaaccaat tagtcactct 277140 cctacttcca ggtatgtaca ttgctcttct ctctgcctggaacagtttcc tccaagtatc 277200 tacatggctt gctccttggc ccttctccag gtccacatcatttgacaagg ccttccctga 277260 atatgctttt taaattgcaa acatccccac cccactaccgtggtgcccag ttcatccttc 277320 cctattttat ttttctctgc agcacttatt ccctcctaatatataatttt cttacctgtt 277380 gtatttattg tttttgtttt tccgctagaa acaaagctctatggtaggag gaattttcag 277440 atatttcatt cttaggttca attcattgct gtatgtctagcacctacaat tgtaactgca 277500 gcattgtgat gctcagcaag tgtttgttaa attaaacaattcaggagact tggctgttat 277560 ggcaagggga tgtagcagta gctagaggag agtgtgaaggagagcacttt ctttttaaca 277620 gtagtgtaaa cttgagcata tttaaatgga gcatgggaaggagatcagag taggaacagc 277680 cccacatcta ttcattcaaa taaataaaat ttctgagcgcattctatggg ccagagaatt 277740 ttctaggcat ttgaaatata cctgtaaaca agaacaaagccattctttat aatcttataa 277800 tccagaagaa tatggaatta ttgattattg aattcttttaagaggtagga gaataagggt 277860 gagagattga ccttaaatat tagtaaagac agtctttcattatggagagt tgtaaaggaa 277920 gtctggatga atgtatagat gcaggtaatt ttaccctgaaccatctaatt ctttcttgct 277980 ttttctcttg cacagtagca taaccaggga tagaaatatattctggatta aataggtttt 278040 ctggtgtctt aaagtataac ccagatactt ttatcttaacacaaagcaga aactccagat 278100 aagaacatgt cgtgaacctg aagccctgta ttatagtagctagcagaacc actcccaata 278160 ttcttagatt tggcattggc agcatatcct ttcttttcctaaagtactaa ctctactact 278220 ctcctaagaa aattatttca catctcgtct ctactcagatctccagcttt tccttattct 278280 tgtacttagc taatggcctt gcttcctaaa aatcagaagagaacatctat tctctttcac 278340 caccatagct actaacttgt ctgcatcaga gtagagtatcacaaaaactg ggggaggagg 278400 atatttcaag aaagagaaag tgattaaaag tgccaaatattgctgggcac ggtggctcac 278460 gcctgtaatc ccagcacttt gggaggctga ggcggggggatcacgaggtc aggagatcga 278520 gaccattctg gctaacatgg agaaaccccg tctctactaaaaatacaaaa aattacgggc 278580 gggcgcctgt agtcccagct actcgggagg ctgaggcaggagaatggcgt gaacccggga 278640 ggagccgaga tcacaccact gcactccagc ctgggtgacagagcaagact ccatctcaaa 278700 aaaaaaaaaa aagtgccaaa tattgagaaa ggtcacaaaaaagtcataaa ttgtccattg 278760 tatttatttt gaaggctact cattatcttg gtgagaatagcttctaagta gagaatagta 278820 cctataaacc ctgccttcct tctgatgatg taaatggacatcatgagcta acgctcttat 278880 ctaatgctag tccctcgatc ctatctcttc ctgcctacttaagaatatga cttggtcggg 278940 tgtggtgact catgcccgta atcccagcaa tttgggaggcccaggtgggt ggatcacttg 279000 ggtcaagagt tcgctaccag cctggctaac acggtgaaaccccgtctcta ctaaaaatgc 279060 aaaaattagc cgggcatggt ggcacatgcc tgtagtcccagctgcttgga aggctgaggc 279120 aggagaatcg cttgaacctg ggaggcgaag attgcagtgtgccgagattg cgccattgca 279180 ctccagcctg ggcgacagag actgagtctc caaaaaaaattatgattcca acaattctct 279240 tatcttttct acatcaattt ttaccttttt gtagtcattatcattgttat gtaaacatat 279300 ttagatatct cctctaaaac aaccctctta caccgggcgtggtggctcac gcctgtaatc 279360 ccagcacttt gggaggccaa ggcgggtgga ttccctgaggtcgggagttc aagaccagcc 279420 tgaccaacat ggagaaaccc catctgtact aaaaatacaaaattagctgg gcgtggtggc 279480 acatgcctgt aatcccaact actcgggagg ctgaggcaggggaattgctt caacccagga 279540 ggcggagttt gcagtgagcc aagatcacac cattgcgctccagcctgggc aacaagagcg 279600 aaactgcgtc ttttaactac attatctaca tggtagccaccatcccactt ttcttttttt 279660 tatagccaac ttacttgaaa agtgacatac caggtatcttccaccgtctt ctttcattct 279720 ctcttgagct cactccagtc atacttttgc attcatcatgctaacagaac aactcttctc 279780 aaggtcagtg gggtcttcct aatagtgact tctcagttctcaccttattt catagcagga 279840 tttgacttag ttgattactc tcttgagaaa atgtcttcatttggcttctg tgacaccaca 279900 tgctgtattt tccttattga actggctact ctttctcagcctcctttgtt cctttttcca 279960 ttctaattgc tgagaccgtt aaaagtttgg agtgctcaagggattactcc ctacttgttc 280020 tcatccagcc ttgtggcttt aaatggtgtc agagtccaagtacatcctcc cttgaattcc 280080 agacttacgt atccagctgt ctaattgaca tctctgcttgagtgttaagc accccacatt 280140 taacgtgtcc agaaaaactt cctgattccc tgaccttgcgaactgctcct tcctgtcttc 280200 ctcatttcag tcctgaacaa ttctgttatt cctagttgtttaagtgaaaa atttagagtc 280260 atcctagact cttctttttt tcttacactc taaatgcagtccaccaacaa attttcccag 280320 tgttaactaa aaattatgta tagactctaa tcacttctcaccacttgtac catttactga 280380 cctggtccaa actgccatca tctccaatct tgattatttcagtagtctcc taactggtat 280440 cccagcttct gccattgtcc cccntactgt ctgatcttaatacagcagcc agaatgatct 280500 ttctaaaaca ccagagtacc agaataaaag tcaaagtccttgtattgcct tggaaagcct 280560 tacagtatct gttgttctct tcccccntac ttttcttctactgttgtctt agcagagagg 280620 cctccttgat caacctgtaa taaaagtgat catattcataactttccata ttttatcttg 280680 tattattctc tgtggcatac tatatagcac ttgttcattttttaaattgc ctgtacggcc 280740 ccactaatgc atgctccctg agggcaggaa ctttgttactgctgtgtctc tttatttatt 280800 tatttattta tttatttttg agacggagtc ttgcactgtcacctcagcct cccaagtagc 280860 cgggattaca ggcacacacc accacacctg gctaattttttgtattttta gtagagatgg 280920 ggttccacta cgttggcaga ctggtctcca actcctgacctcatgatctg cccgcctcag 280980 cctcccaaag ggctgggatt atgggggtga gccactgcttccggcctgct gtatctctta 281040 attcctagaa cagcgtgatg catagtaaac atagtgtagtacttagtaaa ccttcaagta 281100 aatagctgtt gggtgaatta atgaattttc tgtgtgtgtgtatgtgtgtg tgtgtgtgtg 281160 tgtgtgtgtg tgtgtgtgta tgtttactat atgtctccctcttgaggctt caggcagaaa 281220 atttgaattg taatactcac tagaaaatag gaggtggaagttactcattc agaacatatt 281280 tattgagcac ctactctgtt cagggaatac agtagtgactaagagagctt atccaagaga 281340 ggctctttga gaagagtagg caaactggag ttgtcttggtctgaatgtca gttctgaaat 281400 gttttagtat catttccagg gagcattctc agattagtttgtagtgattt gcaaatctct 281460 tataagtttt cataatctca tttaatttca gagctggaagggactagttt aaccatctca 281520 ttttacagat ttgatgaaac tgaactgcag gaagtgtaagggacttgccc agtgtctcta 281580 ctcaattagt taatgataaa acagagataa agttctgggtctagcctgct gattaacaag 281640 tcaatgactt ttccactatg ccacgctgcc ttccattccaaatctgcaag aaatttcttt 281700 tcaaaagcag actatttgct attgctttat gaccttttttagttaagcac aatatgggat 281760 agaaaactaa atagttcttt tataattttt caacatgtgagaggttaaat tcattacttt 281820 tatagaaagn tttgcaatgg atttaaattt attttaagcaataaaataat tctttttatc 281880 atttaaagtt aattgtgttt ttaagaatat gattaagttnggnaaggatg aactgaattt 281940 gtttacatca gtttctctta aaaaattgtt gcagagaaactcttgctact cagttggatc 282000 tagcagaaac aaaagctgag tctngagcag tntggcgcgaggccttctgg aagaacagta 282060 ttttgaantt gacgcaagaa agcnaagaaa gctgcttcaagaaatagaca agagattaca 282120 gataaagatc acactgttag tncgggtaag tgtgatagtcnaggtaagtt atantaaaat 282180 ttatttaaat cacaantagg tcacantcnt taaaatattagggtnaagtg tgtgtgcaag 282240 tgtatttact tctatgttaa tacatagtgg tcattctgtactcatgtata aaatagtttg 282300 tataatacca aacatttgtt aattattgaa attcttgttcaagatattgg gcttttgttt 282360 gtataggcac agaaccaatg caatattggg ctgtttttctggctgtagat cttattagtt 282420 atatttcata tgaagtaatg gcttagctta aattcatttgttgttctctt aatacattgc 282480 aactgatttt atgggccaga attctgtgaa atatagagaaaccatttggg aatgaggaat 282540 tcttgggata atatataaat ccattataag aaaaccaatactccaaacaa ttacctattc 282600 ctgaggttta tactgtaatc tatatagaat catgcgactaaaaagaggag ttagattttt 282660 cttgtaaagc cactatcagt cactctgggt tccagttttgcccctaagaa gttatatggt 282720 cttggcaaag ttggctttaa cttctctaag cctcagatttctcatctata aaatagaaat 282780 agtaatatct cttccacatg attgttatga ggattaaataagacagtaga catagaatga 282840 ttaatataac atataacaag taatgattat atattactaaaagtaatcta taaataaatg 282900 tttttattat cgttttcatc atcaatattc agataaagatattgaagtcc agaaaaagta 282960 tgacttgctc ggggtcacat actaggttaa tcgcagagctagaactaaat acanaatttc 283020 ttagtttgca atctagttat cttgccatta catatgcccccctttntgtt catacatata 283080 tctatatgca tacaatgaaa taatttttgg atgctgttaactacttttaa atatttttga 283140 tgtaattaca tattttaagc tggaaaccag acatttgctgtagttcagca gtgtctgacc 283200 tgtctttatc tagcagtata tttaaaggtt actgcctgaaatacgagaat agcaaagagg 283260 ctggagctta aacttttgtg tgctttgttt agcttgaagaagcaaacagc atgctaacca 283320 aagatattga aatattaaga agagagaatg aagagctaacagagaaaatg aagaaggcag 283380 aggaaggtat gtagaatttt taaaaaatca tcatgttaaatcaaattcag tgatttatct 283440 ttacctatgt tgtgttaggt ttctaagata cctgttttcatatattaagt aacttctgta 283500 gccttaagaa ttatcttaat gaagttactg aaagttactatgtaatgtat acttatttga 283560 ataattaaag acattgttag tttgaaatgc agaaattaaattaataccat acaataacat 283620 ttaatataat ttgttgacag aatataaact ggagaaggaggaggagatca gtgatcttaa 283680 ggctgccttt gaaaagaata tcaacactga acgaacccttaaaacacagg tatacatgct 283740 ttcgatcttt tccacatgtt catttttgag ttaatcattgctctttatgc actttttcct 283800 aaaaccaagg cttataaggt tcaagctttt catcagaacaaataaattct ctccccgcta 283860 actataaaaa acttatttng tgaaatagaa tttcaaggccaggcgcggtg gctcatgcct 283920 gtaatcccaa cactttagga ggctgaggcc agcagaacacttaaggccag gagttcgaga 283980 gcagcctggc tgacatcgaa accccagtct ctactaaaaatacaaaaatt agccgggcgt 284040 ggtggtgncg ctccagtaat accagctagt caggtggctgaggcacgaga attgcttgaa 284100 cccaggaggc agaggttgca gtgagcaaag atcatgccactgcatgccag cctgggcgac 284160 agagcaagac ccagtctcaa aaaaaaaaaa agaaataggatttcagttaa gaaagctaac 284220 ttgcctagta atattgtata gttgtagaag ataagagtatgtggccaagg catagaagct 284280 ttatttgcta gtacccaaca tcagagtagg attagcatactcttgattca gtactgggtt 284340 gtaaagttaa ccaaagaaag gctcctggtt cttacatttaagtagtgtga taagtcagtg 284400 ttagttactt gtgctaccag ttatcatgtc tcaaccatcatgaagttgtc ctaattttca 284460 tacacacaga aagtcctgct tcctgatttt gaagaaaaaaatacatttta gtattaaatt 284520 ggatgactac actattgatc tttattaata atattttaataggctgttaa caaattggca 284580 gaaataatga atcgaaaaga ttttaaaatt gatagaaagaatgctaatac ccaagnattt 284640 gagaaagaaa gaaaaggaaa atcgaaagct gcaactggaactcaaccaag aaagagagaa 284700 attcaaccag atgggtagtg aaancatcag aaggaactgnaatgacatgc naagcggtaa 284760 ggtttctgtg tgggtatgga tatgangtgt gaaggattattttcttaaat tatttctgta 284820 tatacttgtt atctatttat cttgtttaga ttgggaacaatcaagggttc tgcctgtttg 284880 gnntttcaga ctgaaattgg gatatggctg ttataatattaactataatt ggggtagagg 284940 ttttgaagta ttngtagctt atttattttc tctagtcaagactcacnttt tttgaaatca 285000 aaataacaag ncttaatttt ttgatacccc ttggcttgttaatagaccaa aattgagaac 285060 ctggtctggg gcacttagat ctaagctttt tagctccatcagttatggtt ttctgaagtt 285120 ttctatacct ttaaagctac ttcatttgga gccagaaaagcattttcttt tgttgtaact 285180 tttttgnttt gatnggctta tgtttgaaaa caaaaancaaaaactctttt taaggcaagg 285240 ataaaaggat tttgtccgag ctttgtatta ctgagnaaagaacttgacat ttgtgtagtg 285300 tatcaaaatg tgtggtagca aacattcatg aagtttagtgtaccttttta gtattagcca 285360 tttaaattat catttttcga ttagtaatgt tggaaattgttttagcttga ttatattatc 285420 ttgatacttt tccaaattag caattggtag aagaatgtgcacataggaat gagcttcaga 285480 tgcagttggc cagcaaagag agtgatattg agcaattgcgtgctaaactt ttggacctct 285540 cggattctac aagtgttgct agttttccta gtgctgatgaaactgatggt aacctcccag 285600 gtaagaataa ttctgtatca ttcaaacatg tataaatgtcatggaaatta gtgtatattt 285660 ctttcatttg gttttctacc tccttgtcat tttgtccatggattcctaag aaggaattct 285720 tatttctgat agccaaggcc tgatgatgat tatacacttggtgtattttg tggagtagag 285780 cagttgtaag caatcttagg ctatttaaat agacctaagaagctttacct gaataaaaac 285840 cataagaggt ttatgaacct attttgagca gcctaagtccacagtgagag tcaaagctgc 285900 tactacacat ctcactattt tttaaaagtt gtacatttttgtgagtaata gctctttaac 285960 attttatgta ttcttgtgct aaaactaaga actgtactttatcatcagaa ccacagcagc 286020 tacatataga accctatcta gtnggagtag tgatcaacnttcagtcagac tttgctaagt 286080 tttaagtttg gggaaataga taaaatgggt tgggcagtgcagtaagaggc cgggggtgtc 286140 aaataaaggc aggaatgagt ggctaagatt tgagacttttttctgtgtct tctttttccc 286200 agtggagaat caatttaaaa ataaacacaa cctcattcttggtttaatcg tcatgtaaac 286260 tataatacct ttatgtactt tacagagatg agaaatacacatactataag ttcaatttag 286320 aaataaaatg agtaggttat cattattaaa acatgcatttacatatacac agagtcttag 286380 ttcagagtct tggaagtttt gtatacatga taatacacttgaaaatagat tttaattagt 286440 gtattttctg aataatacat aaataggaag atatttatgaagcagcatgt ttgatattgg 286500 gattctgttt taacagatat tacttgttat tgtgaatttgatataaggaa ctagaggagt 286560 cactcagaaa attttatagt atctttcttg gtcaagaaagtgggactcaa agtgggaaat 286620 gaattcagct cctctctgta tgctatgtga tccctggcctcattcttgtg tnggctttgc 286680 taggtcaaaa tcaggtatct gggaatggaa aggcagcgaagaaatatttc ttattttagt 286740 ggtcaaggaa ctcctctgtt tacctatttt tggctctctgaaactgccta cctcttgaga 286800 aacttgcagg tacacacgat tcattattgt aggatcactttgaatttaat aagaacctgt 286860 agctttagtt attgtttgaa catctttcta aagttagacttaatcatcac taagaatatg 286920 acttttagaa gtgtttggct acattctttc aattgttgaactgaattggt aggttagatt 286980 cccacttcat taaccctgag atacttnctg gagttatttagtatagggat tcttactcta 287040 tgtatgtatg tatgtataca ttcttactct atgtatatatgtatgtatgt atgtatgtat 287100 gtatgtatgt atgtatatat gtatgtatgt atgtatgaaacagtctcact ctgttgccca 287160 ggctagagtg cagtggcgtg atcttggttc atgatcctgcctcctgggct caagcgatcc 287220 tcctgcccca acctcctgag tagctgggac tacaggtgcacatcaccaca cccggctaat 287280 ttttttaatt ttttgtagag acgggatttc accatattgcccaggctggt tttgaactcc 287340 tgagctcaag ctatctgcct gccttggcct tccaaagtgctgagattaca ggcatgagcc 287400 acccgcctgg ccgaccttgc tctatttagt gtcagttcgaaatcctgacc aattttattt 287460 ttattccacc catttccctt taactattta taatgccttatatgcacncn ttagtgttct 287520 tagtattngg gttaaatatg tcagaaatgg acacattattggtgaatgct agttagnaag 287580 atttgagttg aaaggtgagc tggtgggact gctaatgaggaaagtgatta attttaccca 287640 ccatcgtggc tgattcagaa aggctaagct catagtgccatcagctggct tcattggcag 287700 acttaggctg gngaatccat ttaggtttca gaatcctgacctaataaatt gacttattga 287760 gcaaagagat ttttaaatgt ttctactagc tccattttcagtatatttct tagaatatta 287820 gtgtactttc aggaattttg tttttaaggt tatcaaagtccaaaccaagt ggggcgggga 287880 gtactggtag ccattctccc ttgctagaag aggccagttaatacctcttt tcctattact 287940 gtgttcttaa ttttgagact ttataaaatc tcaacagtgctgaaccttga ctttcatant 288000 tggaagtaga aattaatgga tagtctttgt caatattatttacctctctg tgggtcagat 288060 attttgctag taaattgaaa atctgtatta aatccaccatttactagatg ctcaaaaatg 288120 gtccctggca aataaagtta aggttttgca ttcatatctatgaagaattg ttaaagaatg 288180 ttgatattca ttcttaatat tagatagtta catatcattcaagtgtctaa aaaatttctt 288240 gaanggtaat ggctaatgat taatttttac ttcagtttagaaaataagta aatgaatata 288300 cgtactaaaa aggagagggc tttgaataga ttaagtttactccaagttaa aatgatggct 288360 ttttgaaata tgggtcacat ttttcagttt ttttttaagtagttggatgg ataaactgac 288420 actgcaactt ttattatttc tcttatattt catcattcagtataaagatt ataatttaat 288480 tatcttgcac atctagaacg cagcctctat gaatccttagtggccatttt gttattaact 288540 agcttaaata ttaaatctcc cttctaaaat tcccaattctaaaaacttta tttaaaaaat 288600 gttactcctt ctctgactta taactctttg aagttcttgaagtcaccttc attctcttaa 288660 ttcttttatg tctttatctt ctctttggtt ctgtagcaggttgtgaccta gcacacaata 288720 caaacataaa gattaccctt acggttatgg gtgcctgttgcctagatgac aaaaaacttt 288780 aatgtgataa ctgtttcttt tcctcattct ttctctttccttcttttgga actgttaacc 288840 tagacatctg aagagaatgt agaaatagat gatggattaggaaaacttgg tattgatact 288900 ccagtaatat tcattatctc ttatggatta tttgtatcatacccttttcc atccagcttg 288960 accttaggtt atgtaactta aacctgaaaa tggtggatctcttggtgtgt ttcccaatat 289020 ttgaatatat gaatgtttct gaccttgccc aaatctagtgatttgaagtt ggtgtatctg 289080 acttgttgag tattaattaa ttttcttttt tatatcacttttgaatttaa ttaaagtgtt 289140 ttgaaatggt tctttagccc tgtttcttaa gcagtatgttttgtggtata gctaaataaa 289200 attaggtagt cttcaaaggt tagctctgat gtttgaattggctatatcga gttttgnnnn 289260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnnnnnnnnnnnn nnnnnnnnnn 289320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttcctttttccat tctaattgct 289380 gagaccgtta aaagtttgga gtgctcaagg gattactccctacttgttct catccagcct 289440 tgtggcttta aatggtgtca gagtccaagt acatcctcccttgaattcca gacttacgta 289500 tccagctgtc taattgacat ctctgcttga gtgttaagcaccccacattt aacgtgtcca 289560 gaaaaacttc ctgattccct gaccttgcga actgctccttcctgtcttcc tcatttcagt 289620 cctgaacaat tctgttattc ctagttgttt aagtgaaaaatttagagtca tcctagactc 289680 ttcttttttt cttacactct aaatgcagtc caccaacaaattttcccagt gttaactaaa 289740 aattatgtat agactctaat cacttctcac cacttgtaccatttactgac ctggtccaaa 289800 ctgccatcat ctccaatctt gattatttca gtagtctcctaactggtatc ccagcttctg 289860 ccattgtccc cctactgtct gatcttaata cagcagccagaatgatcttt ctaaaacacc 289920 agagtaccag aataaaagtc aaagtccttg tattgccttggaaagcctta cagtatctgt 289980 tgttctcttc cccctacttt tcttctactg ttgtcttagcagagaggcct ccttgatcaa 290040 cctgtaataa aagtgatcat attcataact ttccatattttatcttgtat tattctctgt 290100 ggcatactat atagcacttg ttcatttttt aaattgcctgtacggcccca ctaatgcatg 290160 ctccctgagg gcaggaactt tgttactgct gtgtctctttatttatttat ttatttattt 290220 atttatttat ttatttattt atttatttat ttttgagacggagtcttgca ctgtcacctc 290280 agcctcccaa gtagccggga ttacaggcac acaccaccacacctggctaa ttttttgtat 290340 ttttagtaga gatggggttc cactacgttg gcagactggtctccaactcc tgacctcatg 290400 atctgcccgc ctcagcctcc caaagggctg ggattatgggggtgagccac tgcttccggc 290460 ctgctgtatc tcttaattcc tagaacagcg tgatgcatagtaaacatagt gtagtactta 290520 gtaaaccttc aagtaaatag ctgttgggtg aattaatgaattttctgtgt gtatgtgtgt 290580 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtatgtttactatatgtctc cctcttgagg 290640 cttcaggcag aaaatttgaa ttgtaatact cactagaaaataggaggtgg aagttactca 290700 ttcagaacat atttattgag cacctactct gttcagggaatacagtagtg actaagagag 290760 cttatccaag agaggctctt tgagaagagt aggcaaactggagttgtctt ggtctgaatg 290820 tcagttctga aatgttttag tatcatttcc agggagcattctcagattag tttgtagtga 290880 tttgcaaatc tcttataagt tttcataatc tcatttaatttcagagctgg aagggactag 290940 tttaaccatc tcattttaca gatttgatga aactgaactgcaggaagtgt aagggacttg 291000 cccagtgtct ctactcaatt agttaatgat aaaacagagataaagttctg ggtctagcct 291060 gctgattaac aagtcaatga cttttccact atgccacgctgccttccatt ccaaatctgc 291120 aagaaatttc ttttcaaaag cagactattt gctattgctttatgaccttt tttagttaag 291180 cacaatatgg gatagaaaac taaatagttc ttttataatttttcaacatg tgagaggtta 291240 aattcattac ttttatagaa agtttgcaat ggatttaaatttattttaag caataaaata 291300 attcttttta tcatttaaag ttaattgtgt ttttaagaatatgattaagt tggaaggatg 291360 aactgaattt gtttacatca gtttctctta aaaaattgttgcagagaaac tcttgctact 291420 cagttggatc tagcagaaac aaaagctgag tctgagcagttggcgcgagg ccttctggaa 291480 gaacagtatt ttgaattgac gcaagaaagc aagaaagctgcttcaagaaa tagacaagag 291540 attacagata aagatcacac tgttagtcgg gtaagtgtgatagtcaggta agttatataa 291600 aatttattta aatcacaata ggtcacatct taaaatattagggtaagtgt gtgtgcaagt 291660 gtatttactt ctatgttaat acatagtggt cattctgtactcatgtataa aatagtttgt 291720 ataataccaa acatttgtta attattgaaa ttcttgttcaagatattggg cttttgtttg 291780 tataggcaca gaaccaatgc aatattgggc tgtttttctggctgtagatc ttattagtta 291840 tatttcatat gaagtaatgg cttagcttaa attcatttgttgttctctta atacattgca 291900 actgatttta tgggccagaa ttctgtgaaa tatagagaaaccatttggga atgaggaatt 291960 cttgggataa tatataaatc cattataaga aaaccaatactccaaacaat tacctattcc 292020 tgaggtttat actgtaatct atatagaatc atgcgactaaaaagaggagt tagatttttc 292080 ttgtaaagcc actatcagtc actctgggtt ccagttttgcccctaagaag ttatatggtc 292140 ttggcaaagt tggctttaac ttctctaagc ctcagatttctcatctataa aatagaaata 292200 gtaatatctc ttccacatga ttgttatgag gattaaataagacagtagac atagaatgat 292260 taatataaca tataacaagt aatgattata tattactaaaagtaatctat aaataaatgt 292320 ttttattatc gttttcatca ccaatattca gatagagatattgaagtcca gaaaaagtat 292380 gacttgctcg gggtcacata ctaggttaat cgcagagctagaactaaata caaatttctt 292440 agtttgcaat ctagttatct tgccattaca tatgccccccttttgttcat acatatatct 292500 atatgcatac aatgaaataa tttttggatg ctgttaactacttttaaata tttttgatgt 292560 aattacatat tttaagctgg aaaccagaca tttgctgtagttcagcagtg tctgacctgt 292620 ctttatctag cagtatattt aaaggttact gcctgaaatacgagaatagc aaagaggctg 292680 gagcttaaac ttttgtgtgc tttgtttagc ttgaagaagcaaacagcatg ctaaccaaag 292740 atattgaaat attaagaaga gagaatgaag agctaacagagaaaatgaag aaggcagagg 292800 aaggtatgta gaatttttaa aaaatcatca tgttaaatcaaattcagtga tttatcttta 292860 cctatgttgt gttaggtttc taagatacct gttttcatatattaagtaac ttctgtagcc 292920 ttaagaatta tcttaatgaa gttactgaaa gttactatgtaatgtatact tatttgaata 292980 attaaagaca ttgttagttt gaaatgcaga aattaaattaataccataca ataacattta 293040 atataatttg ttgacagaat ataaactgga gaaggaggaggagatcagta atcttaaggc 293100 tgcctttgaa aagaatatca acactgaacg aacccttaaaacacaggtat acatgctttc 293160 gatcttttcc acatgttcat ttttgagtta atcattgctctttatgcact ttttcctaaa 293220 accaaggctt ataaggttca agcttttcat cagaacaaataaattctctc cccgctaact 293280 ataaaaaact tatttgtgaa atagaatttc aaggccaggcgcggtggctc atgcctgtaa 293340 tcccaacact ttaggaggct gaggccagca gaacacttaaggccaggagt tcgagagcag 293400 cctggctgac atcgaaaccc cagtctctac taaaaatacaaaaattagcc gggcgtggtg 293460 gtgcgctcca gtaataccag ctagtcaggt ggctgaggcacgagaattgc ttgaacccag 293520 gaggcagagg ttgcagtgag caaagatcat gccactgcatgccagcctgg gcgacagagc 293580 aagacccagt ctcaaaaaaa aaaaaagaaa taggatttcagttaagaaag ctaacttgcc 293640 tagtaatatt gtatagttgt agaagataag agtatgtggccaaggcatag aagctttatt 293700 tgctagtacc caacatcaga gtaggattag catactcttgattcagtact gggttgtaaa 293760 gttaaccaaa gaaaggctcc tggttcttac atttaagtagtgtgataagt cagtgttagt 293820 tacttgtgct accagttatc atgtctcaac catcatgaagttgtcctaat tttcatacac 293880 acagaaagtc ctgcttcctg attttgaaga aaaaaatacattttagtatt aaattggatg 293940 actacactat tgatctttat taataatatt ttaataggctgttaacaaat tggcagaaat 294000 aatgaatcga aaagatttta aaattgatag aaagaaagctaatacacaag atttgagaaa 294060 gaaagaaaag gaaaatcgaa agctgcaact ggaactcaaccaagaaagag agaaattcaa 294120 ccagatggta gtgaaacatc agaaggaact gaatgacatgcaagcggtaa ggtttctgtg 294180 tgtgtatgga tatgagtgtg aaggattatt ttcttaaattatttctgtat atacttgtta 294240 tctatttatc ttgtttagat tgggaacaat caagggttctgcctgtttgg tttcagactg 294300 aaattgggat atggctgtta taatattaac tataattggggtagaggttt tgaagtattg 294360 tagcttattt attttctcta gtcaagactc acttttttgaaatcaaaata acaagcttaa 294420 ttttttgata ccccttggct tgttaataga acaaaattgagaacctggtc tcgggcactt 294480 agatctaagc tttttagctc catcagttat ggttttctgaagttttctat acctttaaag 294540 ctacttcatt tggagccaga aaagcatttt cttttgttgtaacttttttg ttttgatggc 294600 ttatgtttga aaacaaaaac aaaaactctt tttaaggcaaggagaaaagg aatttgtccg 294660 agcattgtat tactgagaaa gaacttgaca tttctgtagtgtatcaaaat ctgtggtagc 294720 aaacattcat gaagtttact gtacattttt agtattagcaatttaaatta tcatatttca 294780 attagtaatg ttggaaattg ttttagcttg attatattatcttgatactt ttcaaaatta 294840 gcaattggta gaagaatgtg cacataggaa tgagcttcagatgcagttgg ccagcaaaga 294900 gagtgatatt gagcaattgc gtgctaaact tttggacctctcggattcta caagtgttgc 294960 tagttttcct agtgctgatg aaactgatgg taacctcccaggtaagaata attctgtatc 295020 attcaaacat gtataaatgt catggaaatt agtgtatatttctttcattt ggttttctac 295080 ctccttgtca ttttgtccat ggattcctaa gaaggaattcttatttctga tagccaaggc 295140 ctgatgatga ttatacactt ggtgtatttt gtggagtagagcagttgtaa gcaatcttag 295200 gctatttaaa tagacctaag aagctttacc tgaataaaaaccataagagg tttatgaacc 295260 tattttgagc agcctaagtc cacagtgaga gtcaaagctgctactacaca tctcactatt 295320 ttttaaaagt tgtacatttt tgtgagtaat agctctttaacattttatgt attcttgtgc 295380 taaaactaag aactgtactt tatcatcaga accacagcagctacatatag aaccctatct 295440 agtggagtag tgatcaactt cagtcagact ttgctaagttttaagtttgg ggaaatagat 295500 aaaatgggtt gggcagtgca gtaagaggcc gggggtgtcaaataaaggca ggaatgagtg 295560 gctaagattt gagacttttt tctgtgtctt ctttttcccagtggagaatc aatttaaaaa 295620 taaacacaac ctcattcttg gtttaatcgt catgtaaactataatacctt tatgtacttt 295680 acagagatga gaaatacaca tactataagt tcaatttagaaataaaatga gtaggttatc 295740 attattaaaa catgcattta catatacaca gagtcttagttcagagtctt ggaagttttg 295800 tatacatgat aatacacttg aaaatagatt ttaattagtgtattttctga ataatacata 295860 aataggaaga tatttatgaa gcagcatgtt tgatattgggattctgtttt aacagatatt 295920 acttgttatt gtgaatttga tataaggaac tagaggagtcactcagaaaa ttttatagta 295980 tctttcttgg tcaagaaagt gggactcaaa gtgggaaatgaattcagctc ctctctgtat 296040 gctatgtgat ccctggcctc attcttgtgt ggctttgctaggtcaaaatc aggtatctgg 296100 gaatggaaag gcagcgaaga aatatttctt attttagtggtcaaggaact cctctgttta 296160 cctatttttg gctctctgaa actgcctacc tcttgagaaacttgcaggta cacacgattc 296220 attattgtag gatcactttg aatttaataa gaacctgtagctttagttat tgtttgaaca 296280 tctttctaaa gttagactta atcatcacta agaatatgacttttagaagt gtttggctac 296340 attctttcaa ttgttgaact gaattggtag gttagattcccacttcatta accctgagat 296400 acttctggag ttatttagta tagggattct tactctatgtatgtatgtat gtatacattc 296460 ttactctatg tatatatgta tgtatgtatg tatgtatgtatgtatgtatg tatatatgta 296520 tgtatgtatg tatgaaacag tctcactctg ttgcccaggctagagtgcag tggcgtgatc 296580 ttggttcatg atcctgcctc ctgggctcaa gcgatcctcctgccccaacc tcctgagtag 296640 ctgggactac aggtgcacat caccacaccc ggctaatttttttaattttt tgtagagacg 296700 ggatttcacc atattgccca ggctggtttt gaactcctgagctcaagcta tctgcctgcc 296760 ttggccttcc aaagtgctga gattacaggc atgagccacccgcctggccg accttgctct 296820 atttagtgtc agttcgaaat cctgaccaat tttatttttattccacccat ttccctttaa 296880 ctatttataa tgccttatat gcaccttagt gttcttagtattgggttaaa tatgtcagaa 296940 atggacacat tattggtgaa tgctagttag aagatttgagttgaaaggtg agctggtggg 297000 actgctaatg aggaaagtga ttaattttac ccaccatcgtggctgattca gaaaggctaa 297060 gctcatagtg ccatcagctg gcttcattgg cagacttaggctgggaatcc atttaggttt 297120 cagaatcctg acctaataaa ttgacttatt gagcaaagagatttttaaat gtttctacta 297180 gctccatttt cagtatattt cttagaatat tagtgtactttcaggaattt tgtttttaag 297240 gttatcaaag tccaaaccaa gtggggcggg gagtactggtagccattctc ccttgctaga 297300 agaggccagt taatacctct tttcctatta ctgtgttcttaattttgaga ctttataaaa 297360 tctcaacagt gctgaacctt gactttcata ttggaagtagaaattaatgg atagtctttg 297420 tcaatattat ttacctctct gtgggtcaga tattttgctagtaaattgaa aatctgtatt 297480 aaatccacca tttactagat gctcaaaaat ggtccctggcaaataaagtt aaggttttgc 297540 attcatatct aggaagaatt gttaaagaat gttgatattcattcttaata ttagatagtt 297600 acatatcatt caagtgtcta aaaaatttct tgaaggtaatggctaatgat taatttttac 297660 ttcagtttag aaaataagta aatgaatata cgtactaaaaaggagagggc tttgaataga 297720 ttaagtttac tccaagttaa aatgatggct ttttgaaatatgggtcacat ttttcagttt 297780 ttttttaagt agttggatgg ataaactgac actgcaacttttattatttc tcttatattt 297840 catcattcag tataaagatt ataatttaat tatcttgcacatctagaacg cagcctctat 297900 gaatccttag tggccatttt gttattaact agcttaaatattaaatctcc cttctaaaat 297960 tcccaattct aaaaacttta tttaaaaaat gttactccttctctgactta taactctttg 298020 aagttcttga agtcaccttc attctcttaa ttcttttatgtctttatctt ctctttggtt 298080 ctgtagcagg ttgtgaccta gcacacaata caaacataaagattaccctt acggttatgg 298140 gtgcctgttg cctagatgac aaaaaacttt aatgtgataactgtttcttt tcctcattct 298200 ttctctttcc ttcttttgga actgttaacc tagacatctgaagagaatgt agaaatagat 298260 gatggattag gaaaacttgg tattgatact ccagtaatattcattatctc ttatggatta 298320 tttgtatcat acccttttcc atccagcttg accttaggttatgtaactta aacctgaaaa 298380 tggtggatct cttggtgtgt ttcccaatat ttgaatatatgaatgtttct gaccttgccc 298440 aaatctagtg atttgaagtt ggtgtatctg acttgttgagtattaattaa ttttcttttt 298500 tatatcactt ttgaatttaa ttaaagtgtt ttgaaatggttctttagccc tgtttcttaa 298560 gcagtatgtt tgtggtatag ctaaataaat taggtagtcttcagaggtta gctctgatgt 298620 ttgaattggc tatatcgagt attgatgtac atgtagttagattgtctctt atttgggtaa 298680 agaaatcgct gatattgcag taatatacat ctaacttcttataagttaaa attgttctac 298740 aaatatattg tataaaattt agagccaagt tgtttttgtacatatgtaga atattattaa 298800 ggataatcca gacttagagg ttctctcagt gggagtttcatttagttatc tggagaagct 298860 gttttctgtg cttgtgatat aaaagatgga atttacagcgttatacctcc agatcaccac 298920 ttaagttaca ggtggtcccg attttagagg aggtctttatatattttaaa aactggtaag 298980 tatattatct tgcaactatg gtaggcgtaa ggaaaggaaacgcaggagaa gctagtatgt 299040 atttttttat ctgttattat tgagcttagt tcagttgcttctctgaggga attcaccaga 299100 tggaaaaact aaaattttct cccatgacat tgttgtaaattggcaaaaac cttcacatat 299160 tcttgacacc tttatttgca ttccagattt cttcatttaatttattttct ccctgaccct 299220 cttcatttaa tttaaatatg agcaaatgta gaattctggcttatttgact cataagattc 299280 ccttgatata taaaatttct aaggaacact gaaatgacagataaagccat accatataac 299340 aacttattga agatatatta aaactggcag gttaacccacatctagagcc agaaagcata 299400 aatatttttg acattgattg tcacaaagaa ataatgtgaaagtatagggt attttggcat 299460 acaaattttg tgtaaatgtc aagaattcaa aggcttttatatctttgttt ttggtgagga 299520 attactaata ctaaacaact ttgtattatc agatattaagactccttttc attctcacta 299580 atgttcatct taatgttttc caaactacac tttaaaaccagagctgcaat ttctaccaca 299640 ctaatgggaa aatttctggc aataactttt ttgcttttataagataaaat attggtgaat 299700 gatcaatatg tgatcaacag tcttctgcat gctatttagtataaaactgc ctgtttggtt 299760 tttaaactgg aaccactatt tacaaaggat gactttaattggctaattca gaaaaatgca 299820 gaattacaaa ataatcaaca aatatctgtc aagtaccacctcgttgtgtc atttgccatg 299880 ggatatataa aatcaagtgt taagatatgg tccttgctctcacaaaactt accaaggaac 299940 tacctaagca acaaaataat tacttcatga actaaattgcagcaattaaa gccaggtgct 300000 gtggtacatg ccactgctta ggaagctgag gctcaggagtttgaggccag cctggacaac 300060 gtaacaagac ttcatgtcaa aaaaaaaaaa aaaaaaaaaggaaaaagaaa aatgttggga 300120 gaagagaggg atcattttga actaaagtta cctcaccatttcctccattt taacaattta 300180 tatgaagagg aataacacaa gcaaaggtgt ggagacattacgtacctggt gggagtaggg 300240 gaaaaaaata tttcccacaa ttcagtcaac tgcagagcttgtttgcgaac agaatagtgt 300300 tgtgctctag aaacataagc tcagaaatta gactgccggggtttgaaacc atagagcttt 300360 gatagatact gatagagttg ccttgcccaa gtccgtttaccctgctaagc cttagtctct 300420 tcttttgtaa gtgaaagatg acagtagaac cttttccgaattcctcggaa tataatatcc 300480 tgtattcatg gacttataca taggttaaat atgagatattataaagcatg tggcaaatag 300540 taaatttatg aataaataaa tcagtaaatg ttacctattgttaactgtta ctgtaagact 300600 gagtttatag cttttaaatt atatgaaggc ggacaatatattgccttagg attaccattt 300660 gaatgatagt ataatctcat attttagtgt catactttgaaattggttat ttttctattc 300720 ttttgtgttt caaatataag gtagatacgg aaaatagttaaattgaatgg attttcttat 300780 ttccaacctg atctaaaata actatttcta atggagttgtctttatcccc cagatatttc 300840 tagtagacat tagttatatt taaacaatag taataattattataaaatgc cttgatcaaa 300900 tagtcaagga attcctggta tgcacgtact agttgaaataatggcagtag accaaatgga 300960 ttctgtgttg tttttactgt tgatcaaatt gtgatatcagaaacttaaga ggctttaaaa 301020 ctctttagtt tgagtagaat tgcaattgaa cttagcactagagttaagtg ctcccctcaa 301080 ccaaatttgg gcaattttat tgaaatagtt acaaattcaaattgaagttg ttttaccaaa 301140 aagcttaatc agaatattgt actgagaaaa caaataaaatacttgtctct tattctttat 301200 atctagttga ttctgcctgc attccttatt tatttatcttctattcttgt cttctgtgag 301260 taataggaca cttctatact tttgcttgct ctttcttgtgtaaatcatgg ctctgatttg 301320 ttccacttgc tgcattattt ttttctttct ttttttttgctgattatttt tatatgaatg 301380 ttaaatgata aagtcttcta catcatatcc catttaagctgcaatccatt ccagtcttgc 301440 ctggctctta agggtattgt tacttcatac ttttgtaattgattactaaa tgttaaattg 301500 tatgggaaaa gtggcttgga atttttttct gcaatatttgtatggccatt aaagttgatt 301560 tgtgtgattg aagcatggaa gagcaaatta tagcacttattcatttagtt gtgttcaatt 301620 gcttgttgct gcatgctgtc agacaacttt taatagtttttcttttgtta caatttaaga 301680 ctgactaata catatattct ttttctccta gagtcaagaattgaaggttg gctttcagta 301740 ccaaatagag gaaatatcaa acgatatggc tggaagaaacaggtaccata tacctaatta 301800 ttttctaact ggtattttgt tctatatttt actttggagcatagaactta tattagctac 301860 ttttttttac tagcatttat tttacattaa tacagtataatctagatatt agtggcaaat 301920 attatgctta tcactttaaa ccaagttaat gttgctcttgtaactcttcc cccctttttc 301980 ttatttcagt atgttgtggt aagcagcaaa aaaattttgttctataatga cgaacaagat 302040 aaggagcaat ccaatccatc tatggtattg gacatagagtaagttgcctt tgattgaatt 302100 ttaaaggggt tgagagctaa cttttgataa ttatttttagacttaaaatt tattttatat 302160 tttgtgttac ttcagcactg ttacctactt ttctgtttttcttaagcata aatgttgaca 302220 ttatattaaa tcttcagcaa aatgtgagaa ggaaaaatgtatatacttat ttcatgtatt 302280 tggaagacaa cttttttagt tcaagtgttt agtagtttgagttcagagag catttcaaaa 302340 agtatagtca gctgtgtatc agtgggttcc atatccacaactgtggatag aaaatatttg 302400 aaagagggcc gggtgcggtg actcacacct gtaaccccagcactttggga ggtcgaggca 302460 ggtggatcac ctgaggtcag gaattcaaga ccagcctgaccaacatggtg aaaccccatc 302520 tctactaaaa atccaaaaat tagctgggcg tggtggcaggcgcctgtagt cccagctact 302580 cagaaggctg aggcaggaga atcacttgaa cctgggaggcagaggttgca gtgagcctag 302640 attgcgccac tgcactccag cctgggccac aagagcgaaactctgtctca aaaaaaaaat 302700 atatatatta tatgtattat atatattata tataatattatatatattat ataatatata 302760 atatatatat tttatataat atatataata tatatattatataatatata tataatatat 302820 atattatata aaatatatat attatataat ataaaatataatatatatat tatatattat 302880 ataaaatata tatattatat atattatata aaatatatatataaataaat atatatttat 302940 atatataata tatatatttt ataactatta atagaaatttctttaaaaaa tacagtataa 303000 caactgttta catcgcattt atactgtatt atgtcttatgagtaatacag agatgatttt 303060 naaagcatac aggaggatgt gcctaggtta tatgcaaataccancaccat tttatataag 303120 agccttgagc atacttggac tttgatattt ttggangttcctggaatcag ttccctgtgg 303180 atataaaaga acaacntgtg taggattctt tggtcattattaaggggaaa tataaatata 303240 tctgttcttt tgtaatgata ggtcaaagag gcctataatatagtattttt tctntatatt 303300 cattctaaaa tcttttctta attattgctg cttatattatcatagattat gtagtatgta 303360 taaagatgag taaattccat cattaaggac cttaccgactagcaggagaa aaggcaaaac 303420 atattcaaca aaatatatat tcaaagtcaa gtctggtaactgctttttaa aagaaatata 303480 tatataaaat aaagtattat catccctttg gaattaagtatttgtggagc tggttatagt 303540 tagaatgtaa aaagaattaa gcaagttcta gagaatactctttctttatt ttctgttttt 303600 ttaataaaca gctttagttg tcaaaattta aattatcagcttaagcgaaa taatacataa 303660 acactagtat gaaagatact atctttcaac taagttaaatactttttaga ttgatgagag 303720 atttctttcc tctctttatt aaggtataat ttcccacaataaagttcata gatcttaagt 303780 gttctcttca gtaacttttt acagttgtaa acattcatataatcactcaa tacaagattt 303840 aaaaaaatcg tatttcttta gaaaactcac ttgtggctgggcacagtggt tatgcctcta 303900 atcccagcac tttgggagac caaggtgggc ggttggcctgagttcaattt gagaccagcc 303960 tgggcaacat agcgagaccc tgtctctgaa aaaagaattttttttttttt ttttaattta 304020 tgaagaaagc tcacttgggc cagttgtggt ggctcacacctgtaatccca gcactttgga 304080 aggctgaggt gggaggatca cgaggtcagg agttcattcgagaccagtct ggccaacatg 304140 gtgaaaccct gtctctacta aaaatacaaa aaattagctgggcgtggtgg caggtgcctg 304200 taatcccagc tactcgggag gctgaggcag gagaatcgcttgaacccagg aggctgaggt 304260 tgcggtgagc caagattgcg ccactgcact gcagcccgggtgacagagcg agactccatc 304320 tcaaaaaaaa aaaaagaaaa aggaaagctc acttgtgcctcttcccatga tctgatttct 304380 attgccaaga ttagttttgc ctgttctaga actttatataaatggaatta aacagtaatg 304440 tacacttttg tgcctagcat ctttcactca gcctgcacctgtggtggtgt ctgtattgat 304500 tgttctttgt attgctgaat agtataacat tgcatgaatttgtttatcca tttttgtgtt 304560 gattgacatt tgggttgctt ccaatttggg gctatcgtaaataagactta tgagcattcc 304620 tttttttaac ttatgttttc atttctcatg ggtaaatacccaggattgga atcctgggtt 304680 ataaagtaga tctgtgttta actttttaaa aatctaccaaacaattctca aaaattatta 304740 tgtcacacta ttttatattc accaacagta tgagtttcagttgctccaca tcctcaccaa 304800 catttggtga tatcaacctt tgattttagc ttttctagtggatgttaaat gttatttcat 304860 tgttatttta atttgcattc tcctcataac taataataccaagaatcttt catgtcgtta 304920 ttagccattg tatatctttt cttttttcaa gtcttttgcccgtttttaaa ttgggttgct 304980 tatgttttta agattggttt ttaagagttc tagatatattctgaataaga atcctatgtt 305040 agaaatatgt aatgcaaata tttcccaatc tgttgcttacctgttgattt tgttaatggt 305100 gtcttttaat gaggatcagt gtaccaattt ttaaaatgttttctgccttt tgtggtctgt 305160 gtttggtaag aggtggatgt ttaggtcagg ttggcttttttccccccaca taggtatctg 305220 gttgttccaa cagtatttgt ttttatatgt tgaccttgaatcatttaatc ttgctttgca 305280 cttaacagtt ttatagggtt gtggggtttt gggatcaattccacagagct tttctacaca 305340 cagtcagccc tctgtatcca tgggttctgc atctgtatattcatccaaac atggattgaa 305400 aatgtagtta ggcctattgt ggttgtgtct gtacttaatatgtacagttt tgtttttctc 305460 taaacaatat aatataataa caatttccat agcatttacattatattaga tattatctag 305520 agacaatttt aaatatacag gaaggtgtac ttaggttatatacaaatact acaccatttc 305580 ttatcttttc tttttttcct gtctcttccc tccttccctctctttctctc tctttctctt 305640 tcactctctc tcttcaaaat cctcctgggt cctgggaccaatctgccaca gataccacga 305700 gacaactgca tacaatcatg tcatcgccaa ataaagagttttactccttt ctttccaatc 305760 tgtatgcctt tctcacgtgt atagatttgt atgaccaccaccacactcaa aatacagaac 305820 aattccatca caagtgtccc tgtgctactc cttttgtagtcaaaaggcag cttccttcct 305880 ccacctctcc ctaatccata gcaacattaa tctgttctccatctccataa ttttgtctct 305940 tcaagagtgt tatatgaatg gaatcaaata atgtgtaatcttttctgatc agcttttttt 306000 cccattcagt ataattccct taagatcaat ccaagtataatttgtttttt tcttgttgag 306060 tagtattcca tagtatagat gtatcacagt ttgcttacccatttatttgg gttgtttcta 306120 ggttttggtt attatgaata aagctcctat gaacatctatgtgcaggttt ttttaaatag 306180 atataaaatg ttatatgaat ttttgtttct tgaaattaaaggcccaacag tgcaatttgc 306240 tgggttatat ggtaagcata tgtttagttt tctaagaaattgccatattc tttttccaga 306300 gtggctgtgt aattttatgt ttccatcagc agtatatgagtgatttactt tctctgcatt 306360 ctcacaagca tttgatgtta ctactgtttt tattttttttaactctgata gatgtgtagt 306420 gacacttatt gtggttttaa tttgcatttc tctaatgactgatgatgttg aatatctttt 306480 tgtgtgctta tttatcaccc atatgtcctc tttagtgaaatgtcttttac aataaatgag 306540 tttttgtaaa tggcctgtat ttattttcat tttagtaaactgtttcacgt tagacctgta 306600 acccaaggag atgtgtatag agctgaaact gaagaaattcctaaaatatt ccaggtaaaa 306660 atctgaagtt ataattttaa agaatctaag tcctaagcgtttttgtgaag taaatgtaaa 306720 atataccaat gcttaagatt tttaaaatct tttcctttcaccattcctga atgtagatac 306780 tatatgcaaa tgaaggtgaa tgtagaaaag atgtagagatggaaccagta caacaagctg 306840 aaaaaactaa tttccaaaat cacaaaggcc atgagtttattcctacactc taccactttc 306900 ctgccaattg tgatgcctgt gccaaacctc tctggcatgtttttaagcca ccccctgccc 306960 tagagtgtcg aagatgccat gttaagtgcc acagagatcacttagataag aaagaggact 307020 taatttgtcc atgtaaaggt aaggcatggt aattattgttacaacttata acaaatttaa 307080 ttttaagtct caatgaacta acttatgtgt agtgatttctataccaagtt gaaaagagat 307140 tatagaaagt attattttga cactggtggg gcaatatgcagctatttaca gatttagctt 307200 ttaaaaatgt ttttatttat ttttattgat tgactgattgattgagatgg attctcactc 307260 tgtctcgccc aggctggagt gcagtggcgt gatctcagctcactgcagcc tctgcctccc 307320 aggttcaagt gattctccta tctcagcctc ctgagtagctgggactatag gcgtgtgcca 307380 ccacacccgg ctaattttgt atttttagta gagatggagtttcaccatgt tgtccaggct 307440 ggtttcgaac tcctgacatc aggtgatcca cccacctcggcctcccaaag tgctgggatt 307500 acaggtgtga gccattgcac gcccggccaa cagtgtttttaaagagaatc acccccttgt 307560 aaacaaacaa aaacaaacaa acatgctgct aagaaattattccaagttgg agagaagagc 307620 aacaggtgag ttccactcta ttcctccctt gagtttttcctcttcttttt gtctggcatt 307680 cactgcctgg atcagcatgc atgctgtatc acagaatatcttctagtttt acagaggtag 307740 cttgagggaa agaatacaga ccatagctat tttcagataagaagcttatg ttctttaaat 307800 acatttattt gttttacatt ttgttatatt tctgattttactttattagt ttgactaatt 307860 ttttttttta atttcagcct tatttaactt aaggagaatgagatattgtt catgcttaat 307920 taagcatatt aaaactttta aaaccttaag aacttgaaaaactatgcatt tgataggttg 307980 gattttaaaa aactatatgg ctttaattat gctttttttttcctccttaa catagtaagt 308040 tatgatgtaa catcagcaag agatatgctg ctgttagcatgttctcagga tgaacaaaaa 308100 aaatgggtaa ctcatttagt aaagaaaatc cctaagaatccaccatctgg ttttgttcgt 308160 gcttcccctc gaacgctttc tacaagatcc actgcaaatcagtctttccg gaaagtggtc 308220 aaaaatacat ctggaaaaac taggtaagga tttgaaatgctaacgttttt aacaatgtga 308280 aaatgattgg ataacagcta ttaaaatcag aaatctaattgtgcttttca ccacagaggg 308340 catcattttt aggtatttgt atgtgaattg cagtgactttataaattagt ttaacttatt 308400 cttgttagag tttaataata ataatacatg caatattatatttatttagt tctgtgtcta 308460 attttattgt cataagttgc tgtacatggc atagttttagcccttaatgc tttaaccaac 308520 ttaatttttt taatgcagaa attcatataa aacttttcatcatcttttct gttttttaac 308580 tgtgttggca tagtttttct acaaaaacca aaattgtttttccctttttt gttaacttcc 308640 catactatcc caacatacaa aattatttgg tattatcagtgactatgttt ttttaattat 308700 tatactttaa gttctgggat atatgtgcag aacatgcaggtttgttacat gggtatacac 308760 gtgccatggt ggtttgctgc acccatcaac ccatcatctacattaggtat ttctcctaat 308820 gctatccctg ccctagcccc ccatccctcg acaggccctggtgcgtgacg ttcccctccc 308880 tgtgtccatg ttttctcatt gttcaactcc cacttacgagtgagaacctg tggtgtttgg 308940 ttttctattc ctgtgttagt ttgctgagaa tgatggtttccagcttcatc catgtccctt 309000 caaaggatgt gaactcatga cccccttttt ttttttgttttttgagacag agtctcactc 309060 tgtcacccag gctggagtgc agtggtggcg ctatctcagctcactgcaac ctccacctcc 309120 caagttcaag caattctcca gcctcagcct cccaagtagcggggattaca gacgcatgcc 309180 acgcctggct aatttttgta tttttagtag agagagggtttcatcatgtt ggccaggctg 309240 atctcaaact cctgacctca aatgatctgc atgccccagcctcccaaagt gctgggatta 309300 caggcgtgag ccaccatgcc tggctgttta actatttttgatggactttt ttccaaaaat 309360 atgttttatg ccatgataaa caattataag gcataaccattcattcattc attcattcat 309420 tcattcattc attcattttg agacagagtc tcgctctgtcacccaggcca gagtgcagtg 309480 gcgcaatctc agctcactgc aacctctgcc tcccaggttcaagctattct cccacctcag 309540 cctcctgagt agctggaatt acaggcacat gccaccacggcctgctaatt tttgtatttt 309600 tagtagagat ggagtttaac atgttggcca ggctggtcttgaactcctga cctcaggtga 309660 tgcaccttcc tgggcctccc aaagtgctgc gattacaggcatgagccacc gcaccaggca 309720 gttaactctt tcttggagag aaataatgaa atctcatctcattaagttct cattttacac 309780 attagtaaat aaggctcaga gaaattaagc agtttgctcaatcacacagc caatggattg 309840 gaaaccattt ctgtccaatt ctaaagccta tgttgtccaattccaaagcc tctattctac 309900 cacataccac cttccctaga gtgtactccc tagattaatagggccactcg aattagaagc 309960 tgagacctaa cactgatttg cctcgctgcc aatgtcacctacatcttcca agcaggctta 310020 aagccatctt gaattcatcc cttttgttca tgccctattgtcagtaacca ggcctaattc 310080 tttgaaatga cttatatcct ttcctttctc agccaccatactatgccagg tattatcacc 310140 ttactgctaa cttaattctg tagaatgcta tgtcagccttctcctgagca taggttgtaa 310200 actggatcta aaatgatttg aaatgagatt atagctgagtaagaaaatgt gtagaaacaa 310260 agaggagaga atactaagta tatttttcct attccttgttttccaaattt tgtgtaattg 310320 ttaattattt tatgtaaaga taatgtatat ttacctttcctcctaatgtt tgtttcttgt 310380 atttttaaac tgagtgcatt tcttaaatta gttttttccccctccctcaa cagttaacca 310440 tgtgactgag tgccctgtgg aatcgtgtgg gatgctacctgataaaccag gcttctttaa 310500 ccatgcagag cagacaggct gtttctttga cacaaatatcacaggcttca gggttaagat 310560 tgctgttttt ctgtccttgc tttggcacaa cacactgagggtttttttta ttgcgggttt 310620 gcctacaggt agattagatt aattattact atgtaatgcaagtacagttg ggggaaagct 310680 taggtagata tatttttttt aaaaggtgct gcctttttggatttataaga aaatgcctgt 310740 cagtcgtgat agaacagagt tttcctcata tgagtaagaggaagggactt tcactttcaa 310800 gtggaacagc catcactatc aagatcagct catggaaggagtaaagaaaa tatctcaaaa 310860 tgagacaaac tgaagttttg tttttttttt aatgacttaagtttttgtgc tcttgcaaga 310920 ctatacaaaa ctattttaag aaagcagtga tatcacttgaacttcagtgc cctcactgta 310980 gaatttaaaa gccttactgt tgattgccca tgttggacttgatggagaaa ttaaatatct 311040 ttcattatgc tttacaaaat actgtatatg tttcagcaagtttggggaat gggagaggac 311100 aaaaaaaagt tacatttaat ctatgcattt ttgccaagccatattgagtt attttactac 311160 tagagacatt aggaaactaa ctgtacaaaa gaaccaagtttaaaagcatt ttgtggggta 311220 catcatttct ataattgtat aatgtatttc tttgtggttttaaatgataa agacattaag 311280 ttaacaaaca tataagaaat gtatgcactg tttgaaatgtaaattattct tagaacactt 311340 tcaatggggg ttgcattgtc cttttagtgc cttaatttgagataattatt ttactgccat 311400 gagtaagtat agaaatttca aaaaatgtat tttcaaaaaattatgtgtgt cagtgagttt 311460 ttcattgata attggtttaa tttaaaatat ttagaggtttgttggacttt cataaattga 311520 gtacaatctt tgcatcaaac tacctgctac aataatgactttataaaact gcaaaaaatg 311580 tagaaggttg caccaacata aaaaggaaat atggcaatacatccatgatg ttttccagtt 311640 aacataggaa ttaccagata aatactgtta aactcttgtccagtaacaag agttgattca 311700 tatggacagt atgatttatt gtttattttt ttaaccaaatacctcctcag taatttataa 311760 tggctttgca gtaatgtgta tcagataaga agcactggaaaaccgatcgt ctctaggatg 311820 atatgcatgt ttcaagtggt attgaaagcc gcactgatggatatgtaata ataaacatat 311880 ctgttattaa tatactaatg actctgtgct catttaatgagaaataaaag taatttatgg 311940 atgggtatct ttaattttta ctgcaatgtg ttttctcatggctgaaatga atggaaaaca 312000 tacttcaaat tagtctctga ttgtatataa atgtttgtgaaattccatgg ttagattaaa 312060 gtgtattttt aaaagataaa acgtggtctt gggctctaaaacaagagtgg gaatagttca 312120 catgaaaagc tggctaaagt gtgttggaaa acaattctccatgggtctct tgcatttcta 312180 cacatcaggg aagcagaggc tctgacaacc tttcttttgactatcttttc caaggatatt 312240 tatgtagtaa acagccttgg aagatagagt tctcccttgtgaggatgtcg ggttctttta 312300 ctgtccagta taataaagat aacgtctccc acctatgatgcaaacccact gtgtgctcag 312360 cacccacctg ggccactctg cattgcccac atggggcttggagggcaaag ggaactgatg 312420 gaacgtgaaa cttatgctgc ttttgctgtg ctgtgaatgaagtcatctga ctctgac 312477 <210> SEQ ID NO 13 <211> LENGTH: 2190 <212>TYPE: DNA <213> ORGANISM: H. sapiens <220> FEATURE: <221> NAME/KEY: CDS<222> LOCATION: (121)...(477) <400> SEQUENCE: 13 aaactgtttc acgttagacctgtaacccaa ggagatgtgt atagagctga aactgaagaa 60 attcctaaaa tattccagatactatatgca aatgaaggtg aatgtagaaa agatgtagag 120 atg gaa cca gta caa gaagct gaa aaa act aat ttc caa aat cac aaa 168 Met Glu Pro Val Gln Glu AlaGlu Lys Thr Asn Phe Gln Asn His Lys 1 5 10 15 ggc cat gag ttt att cctaca ctc tac cac ttt cct gcc aat tgt gat 216 Gly His Glu Phe Ile Pro ThrLeu Tyr His Phe Pro Ala Asn Cys Asp 20 25 30 gcc tgt gcc aaa cct ctc tggcat gtt ttt aag cca ccc cct gcc cta 264 Ala Cys Ala Lys Pro Leu Trp HisVal Phe Lys Pro Pro Pro Ala Leu 35 40 45 gag tgt caa aga cgc cat gtt aagtgc cac aga gat cac tta gat aag 312 Glu Cys Gln Arg Arg His Val Lys CysHis Arg Asp His Leu Asp Lys 50 55 60 aaa gag gac tta att tgt cca tgt aaagta agt tat gat gta aca tca 360 Lys Glu Asp Leu Ile Cys Pro Cys Lys ValSer Tyr Asp Val Thr Ser 65 70 75 80 gca aga gat atg ctg ctg tta gca tgttct cag gat gaa caa aaa aaa 408 Ala Arg Asp Met Leu Leu Leu Ala Cys SerGln Asp Glu Gln Lys Lys 85 90 95 tgg gta act cat tta gta aag aaa atc cctaag aat cca cca tct ggt 456 Trp Val Thr His Leu Val Lys Lys Ile Pro LysAsn Pro Pro Ser Gly 100 105 110 ttt gtt cgt gct tcc cct tga acgctttctacaagatccac tgcaaatcag 507 Phe Val Arg Ala Ser Pro 115 tctttccggaaagtggtcaa aaatacatct ggaaaaacta gttaaccatg tgaccgagtg 567 ccttgtggaatcgtgtggga tgctacctga taaaccaggc ttctttaacc atgcagagca 627 gacaggctgtttctttgaca caaatatcac aggcttcagg gttaagattg ctgtttttct 687 gtccttgctttggcacaaca cactgagggt tttttttatc gcgggtttgc ctacaggtag 747 attagattaattattactat gtaatgcaag tacagttggg ggaaagctta ggtagatata 807 ttttttttaaaaggtgctgc ctttttggat ttataagaaa atggctgtca gtcgtgatag 867 aacagagttttcctcatatg agtaagagga agggactttc actttcaagt ggaacggcca 927 tcactatcaagatcagctca tggaaggagt aaagaaaata tctcaaaatg agacaaactg 987 aagttttgttttttttttaa tgacttaagt ttttgtgctc ttgcaagact atacaaaact 1047 attttaagaaagcagtgata tcacttgaac ttcagtgccc tcactgtaga atttaaaagc 1107 cttactgttgattgcccatg gtggacttga tggagaaatt aaatatcttt cattatgctt 1167 tacaaaatactgtatatgtt tcagcaagtt tggggaatgg gagaggacga aaaaaagtta 1227 catttaatctatgcattttt gccaagccat attgagttat tttactacta gagacattag 1287 gaaactaactgtacaaaaga accaagttta aaagcatttt gtggggtaca tcatttctat 1347 aattgtataatgtatttctt tgtggtttta aatgataaag acattaagtt aacaaacata 1407 taagaaatgtatgcactgtt tgaaatgtaa attattctta gaacactttc aatgggggtt 1467 gcattgtccttttagtgcct taatttgaga taattatttt actgccatga gtaagtatag 1527 aaatttcaaaaaatgtattt tcaaaaaatt atgtgtgtcc gtgagttttt cattgataat 1587 tggtttaatttaaaatattt agaggtttgt tggactttca taaattgagt acaatctttg 1647 catcaaactacctgctacaa taatgacttt ataaaactgc aaaaaatgta gaaggttgca 1707 ccaatataaaaaggaaatat ggcaatacat ccatgatgtt ttccagttaa cataggaatt 1767 accagataaatactgttaaa ctcttgtccg gtaacaagag ttgattcata tggacagtat 1827 gatttattgtttcttttttt aaccaaatac ctcctcagta atttataatg gctttgcagt 1887 aatgtctatcagataagaag cactggaaaa ccgatcgtct ctaggatgat atgcatgttt 1947 caagtggtattgaaagccgc actgatgaat atgtaataat aaacatatct gttattaata 2007 tagtaatgactgtgctcatt taatgagaaa taaaagtaat ttatggatgg atatctttaa 2067 ttttgactgcaatgtgtttt ctcatggctg aaatgaatgg aaaacatact tcaaattagt 2127 ctctgattgtatataaatgt ttgtgaaatt ccatggttag attaaagtat atttttaaaa 2187 gat 2190<210> SEQ ID NO 14 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM:Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 14 aaactgtccc cagtcgacat 20 <210> SEQ IDNO 15 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 15 tctcaaaact gtccccagtc 20 <210> SEQ IDNO 16 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 16 ggatcccgca gcaggttgtc 20 <210> SEQ IDNO 17 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 17 atttgggatc ccgcagcagg 20 <210> SEQ IDNO 18 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 18 ttccgatttg ggatcccgca 20 <210> SEQ IDNO 19 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 19 ttcacttccg atttgggatc 20 <210> SEQ IDNO 20 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 20 aatccatcca gcaaacaatc 20 <210> SEQ IDNO 21 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 21 catccaatcc atccagcaaa 20 <210> SEQ IDNO 22 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 22 tcttaaggca ggaaaatcca 20 <210> SEQ IDNO 23 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 23 ttgaacttct ccaaatgcac 20 <210> SEQ IDNO 24 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 24 gatgtccctt tcttcccaga 20 <210> SEQ IDNO 25 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 25 tacttctgca gtatagaatc 20 <210> SEQ IDNO 26 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 26 gaataaaacc catggaatgg 20 <210> SEQ IDNO 27 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 27 gctaacttca aatgtccaga 20 <210> SEQ IDNO 28 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 28 tattcatctt catacaagta 20 <210> SEQ IDNO 29 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 29 ccaaccaaag aatctgcata 20 <210> SEQ IDNO 30 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 30 aagtgaattt ttatggttca 20 <210> SEQ IDNO 31 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 31 gatatgtcat tatcatcagg 20 <210> SEQ IDNO 32 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 32 tcatttttga agaagagatg 20 <210> SEQ IDNO 33 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 33 gctacagtgt ctcggagcgt 20 <210> SEQ IDNO 34 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 34 ctggtgctac agtgtctcgg 20 <210> SEQ IDNO 35 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 35 tcaatgtcac tacttaaatc 20 <210> SEQ IDNO 36 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 36 tagtatcaat gtcactactt 20 <210> SEQ IDNO 37 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 37 attactagta tcaatgtcac 20 <210> SEQ IDNO 38 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 38 tcaaaattac tagtatcaat 20 <210> SEQ IDNO 39 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 39 agtcatcaaa attactagta 20 <210> SEQ IDNO 40 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 40 gtaaatccta caaaaggtag 20 <210> SEQ IDNO 41 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 41 tataatatgt aaatcctaca 20 <210> SEQ IDNO 42 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 42 attgctataa tatgtaaatc 20 <210> SEQ IDNO 43 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 43 ctacgattgc tataatatgt 20 <210> SEQ IDNO 44 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 44 agtatctacg attgctataa 20 <210> SEQ IDNO 45 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 45 tggtactcat taattctatg 20 <210> SEQ IDNO 46 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 46 tgtagaaact tcattttcta 20 <210> SEQ IDNO 47 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 47 ttctcattag caagctgtga 20 <210> SEQ IDNO 48 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 48 tctgtcctaa gtaagtcatt 20 <210> SEQ IDNO 49 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 49 atctctgtgt gactcttcct 20 <210> SEQ IDNO 50 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 50 tgctcatctc tgtgtgactc 20 <210> SEQ IDNO 51 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 51 tgacttgctc atctctgtgt 20 <210> SEQ IDNO 52 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 52 ccaatcatct cagaatcatg 20 <210> SEQ IDNO 53 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 53 ggtctccaat catctcagaa 20 <210> SEQ IDNO 54 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 54 ttgaaggtct ccaatcatct 20 <210> SEQ IDNO 55 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 55 catgtcttga gcctcttttc 20 <210> SEQ IDNO 56 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 56 gccttctctc gagcttctct 20 <210> SEQ IDNO 57 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 57 aataaagtat ttatttcctg 20 <210> SEQ IDNO 58 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 58 cttccaataa agtatttatt 20 <210> SEQ IDNO 59 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 59 tgagctaact caaattctaa 20 <210> SEQ IDNO 60 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 60 taagctgagc taactcaaat 20 <210> SEQ IDNO 61 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 61 tctatactgt ttcgtaagct 20 <210> SEQ IDNO 62 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 62 gactcagctt ttgtttctgc 20 <210> SEQ IDNO 63 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 63 gctcagactc agcttttgtt 20 <210> SEQ IDNO 64 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 64 aaaatactgt tcttccagaa 20 <210> SEQ IDNO 65 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 65 gaagcagctt tcttgctttc 20 <210> SEQ IDNO 66 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 66 ttcttgaagc agctttcttg 20 <210> SEQ IDNO 67 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 67 tctatttctt gaagcagctt 20 <210> SEQ IDNO 68 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 68 acagcctgtg ttttaagggt 20 <210> SEQ IDNO 69 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 69 tgttaacagc ctgtgtttta 20 <210> SEQ IDNO 70 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 70 attgcgcttg catgtcattc 20 <210> SEQ IDNO 71 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 71 taccaattgc gcttgcatgt 20 <210> SEQ IDNO 72 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 72 tcttctacca attgcgcttg 20 <210> SEQ IDNO 73 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 73 ctgtttcttc cagccatatc 20 <210> SEQ IDNO 74 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 74 acatactgtt tcttccagcc 20 <210> SEQ IDNO 75 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 75 ccacaacata ctgtttcttc 20 <210> SEQ IDNO 76 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 76 atctccttgg gttacaggtc 20 <210> SEQ IDNO 77 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 77 tacacatctc cttgggttac 20 <210> SEQ IDNO 78 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 78 tatctggaat attttaggaa 20 <210> SEQ IDNO 79 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 79 ctacattcac cttcatttgc 20 <210> SEQ IDNO 80 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 80 cttcgacact ctagggcagg 20 <210> SEQ IDNO 81 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 81 ggcatcttcg acactctagg 20 <210> SEQ IDNO 82 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 82 aacatggcat cttcgacact 20 <210> SEQ IDNO 83 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 83 tctcttgctg atgttacatc 20 <210> SEQ IDNO 84 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 84 cggaaagact gatttgcagt 20 <210> SEQ IDNO 85 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 85 actagttttt ccagatgtat 20 <210> SEQ IDNO 86 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 86 tcctgttcaa acaaacggag 20 <210> SEQ IDNO 87 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 87 gggagtgaaa aatcccagtc 20 <210> SEQ IDNO 88 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 88 aataacctac caattgaact 20 <210> SEQ IDNO 89 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 89 gttggaaacg ggaatatcat 20 <210> SEQ IDNO 90 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 90 ggcaacttac tctatgtcca 20 <210> SEQ IDNO 91 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ArtificialSequence <220> FEATURE: <223> OTHER INFORMATION: AntisenseOligonucleotide <400> SEQUENCE: 91 cacgactgac agccattttc 20 <210> SEQ IDNO 92 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 92 atgtcgactg gggacagttt 20 <210> SEQ ID NO 93<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 93 gactggggac agttttgaga 20 <210> SEQ ID NO 94<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 94 gacaacctgc tgcgggatcc 20 <210> SEQ ID NO 95<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 95 cctgctgcgg gatcccaaat 20 <210> SEQ ID NO 96<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 96 tgcgggatcc caaatcggaa 20 <210> SEQ ID NO 97<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 97 gatcccaaat cggaagtgaa 20 <210> SEQ ID NO 98<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 98 gattgtttgc tggatggatt 20 <210> SEQ ID NO 99<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 99 tttgctggat ggattggatg 20 <210> SEQ ID NO 100<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 100 tggattttcc tgccttaaga 20 <210> SEQ ID NO101 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 101 gtgcatttgg agaagttcaa 20 <210> SEQ ID NO102 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 102 tctgggaaga aagggacatc 20 <210> SEQ ID NO103 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 103 gattctatac tgcagaagta 20 <210> SEQ ID NO104 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 104 ccattccatg ggttttattc 20 <210> SEQ ID NO105 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 105 tctggacatt tgaagttagc 20 <210> SEQ ID NO106 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 106 tacttgtatg aagatgaata 20 <210> SEQ ID NO107 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 107 tatgcagatt ctttggttgg 20 <210> SEQ ID NO108 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 108 tgaaccataa aaattcactt 20 <210> SEQ ID NO109 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 109 cctgatgata atgacatatc 20 <210> SEQ ID NO110 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 110 catctcttct tcaaaaatga 20 <210> SEQ ID NO111 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 111 acgctccgag acactgtagc 20 <210> SEQ ID NO112 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 112 ccgagacact gtagcaccag 20 <210> SEQ ID NO113 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 113 gatttaagta gtgacattga 20 <210> SEQ ID NO114 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 114 aagtagtgac attgatacta 20 <210> SEQ ID NO115 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 115 gtgacattga tactagtaat 20 <210> SEQ ID NO116 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 116 attgatacta gtaattttga 20 <210> SEQ ID NO117 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 117 tactagtaat tttgatgact 20 <210> SEQ ID NO118 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 118 ctaccttttg taggatttac 20 <210> SEQ ID NO119 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 119 acatattata gcaatcgtag 20 <210> SEQ ID NO120 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 120 ttatagcaat cgtagatact 20 <210> SEQ ID NO121 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 121 catagaatta atgagtacca 20 <210> SEQ ID NO122 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 122 tagaaaatga agtttctaca 20 <210> SEQ ID NO123 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 123 tcacagcttg ctaatgagaa 20 <210> SEQ ID NO124 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 124 aatgacttac ttaggacaga 20 <210> SEQ ID NO125 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 125 aggaagagtc acacagagat 20 <210> SEQ ID NO126 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 126 gagtcacaca gagatgagca 20 <210> SEQ ID NO127 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 127 acacagagat gagcaagtca 20 <210> SEQ ID NO128 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 128 catgattctg agatgattgg 20 <210> SEQ ID NO129 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 129 ttctgagatg attggagacc 20 <210> SEQ ID NO130 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 130 agatgattgg agaccttcaa 20 <210> SEQ ID NO131 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 131 gaaaagaggc tcaagacatg 20 <210> SEQ ID NO132 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 132 agagaagctc gagagaaggc 20 <210> SEQ ID NO133 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 133 caggaaataa atactttatt 20 <210> SEQ ID NO134 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 134 aataaatact ttattggaag 20 <210> SEQ ID NO135 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 135 ttagaatttg agttagctca 20 <210> SEQ ID NO136 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 136 atttgagtta gctcagctta 20 <210> SEQ ID NO137 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 137 agcttacgaa acagtataga 20 <210> SEQ ID NO138 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 138 gcagaaacaa aagctgagtc 20 <210> SEQ ID NO139 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 139 aacaaaagct gagtctgagc 20 <210> SEQ ID NO140 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 140 ttctggaaga acagtatttt 20 <210> SEQ ID NO141 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 141 gaaagcaaga aagctgcttc 20 <210> SEQ ID NO142 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 142 caagaaagct gcttcaagaa 20 <210> SEQ ID NO143 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 143 aagctgcttc aagaaataga 20 <210> SEQ ID NO144 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 144 acccttaaaa cacaggctgt 20 <210> SEQ ID NO145 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 145 taaaacacag gctgttaaca 20 <210> SEQ ID NO146 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 146 gaatgacatg caagcgcaat 20 <210> SEQ ID NO147 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 147 acatgcaagc gcaattggta 20 <210> SEQ ID NO148 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 148 caagcgcaat tggtagaaga 20 <210> SEQ ID NO149 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 149 gatatggctg gaagaaacag 20 <210> SEQ ID NO150 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 150 ggctggaaga aacagtatgt 20 <210> SEQ ID NO151 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 151 gaagaaacag tatgttgtgg 20 <210> SEQ ID NO152 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 152 gacctgtaac ccaaggagat 20 <210> SEQ ID NO153 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 153 gtaacccaag gagatgtgta 20 <210> SEQ ID NO154 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 154 ttcctaaaat attccagata 20 <210> SEQ ID NO155 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 155 gcaaatgaag gtgaatgtag 20 <210> SEQ ID NO156 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 156 cctgccctag agtgtcgaag 20 <210> SEQ ID NO157 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 157 cctagagtgt cgaagatgcc 20 <210> SEQ ID NO158 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 158 agtgtcgaag atgccatgtt 20 <210> SEQ ID NO159 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 159 gatgtaacat cagcaagaga 20 <210> SEQ ID NO160 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 160 actgcaaatc agtctttccg 20 <210> SEQ ID NO161 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 161 atacatctgg aaaaactagt 20 <210> SEQ ID NO162 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 162 tggacataga gtaagttgcc 20 <210> SEQ ID NO163 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: H. sapiens <220>FEATURE: <400> SEQUENCE: 163 gaaaatggct gtcagtcgtg 20

What is claimed is:
 1. A compound 8 to 80 nucleobases in length targeted to a nucleic acid molecule encoding ROCK 1, wherein said compound specifically hybridizes with said nucleic acid molecule encoding ROCK 1 (SEQ ID NO: 4) and inhibits the expression of ROCK
 1. 2. The compound of claim 1 comprising 12 to 50 nucleobases in length.
 3. The compound of claim 2 comprising 15 to 30 nucleobases in length.
 4. The compound of claim 1 comprising an oligonucleotide.
 5. The compound of claim 4 comprising an antisense oligonucleotide.
 6. The compound of claim 4 comprising a DNA oligonucleotide.
 7. The compound of claim 4 comprising an RNA oligonucleotide.
 8. The compound of claim 4 comprising a chimeric oligonucleotide.
 9. The compound of claim 4 wherein at least a portion of said compound hybridizes with RNA to form an oligonucleotide-RNA duplex.
 10. The compound of claim 1 having at least 70% complementarity with a nucleic acid molecule encoding ROCK 1 (SEQ ID NO: 4) said compound specifically hybridizing to and inhibiting the expression of ROCK
 1. 11. The compound of claim 1 having at least 80% complementarity with a nucleic acid molecule encoding ROCK 1 (SEQ ID NO: 4) said compound specifically hybridizing to and inhibiting the expression of ROCK
 1. 12. The compound of claim 1 having at least 90% complementarity with a nucleic acid molecule encoding ROCK 1 (SEQ ID NO: 4) said compound specifically hybridizing to and inhibiting the expression of ROCK
 1. 13. The compound of claim 1 having at least 95% complementarity with a nucleic acid molecule encoding ROCK 1 (SEQ ID NO: 4) said compound specifically hybridizing to and inhibiting the expression of ROCK
 1. 14. The compound of claim 1 having at least one modified internucleoside linkage, sugar moiety, or nucleobase.
 15. The compound of claim 1 having at least one 2′-O-methoxyethyl sugar moiety.
 16. The compound of claim 1 having at least one phosphorothioate internucleoside linkage.
 17. The compound of claim 1 having at least one 5-methylcytosine.
 18. A method of inhibiting the expression of ROCK 1 in cells or tissues comprising contacting said cells or tissues with the compound of claim 1 so that expression of ROCK 1 is inhibited.
 19. A method of screening for a modulator of ROCK 1, the method comprising the steps of: a. contacting a preferred target segment of a nucleic acid molecule encoding ROCK 1 with one or more candidate modulators of ROCK 1, and b. identifying one or more modulators of ROCK 1 expression which modulate the expression of ROCK
 1. 20. The method of claim 19 wherein the modulator of ROCK 1 expression comprises an oligonucleotide, an antisense oligonucleotide, a DNA oligonucleotide, an RNA oligonucleotide, an RNA oligonucleotide having at least a portion of said RNA oligonucleotide capable of hybridizing with RNA to form an oligonucleotide-RNA duplex, or a chimeric oligonucleotide.
 21. A diagnostic method for identifying a disease state comprising identifying the presence of ROCK 1 in a sample using at least one of the primers comprising SEQ ID Nos 5 or 6, or the probe comprising SEQ ID NO:
 7. 22. A kit or assay device comprising the compound of claim
 1. 23. A method of treating an animal having a disease or condition associated with ROCK 1 comprising administering to said animal a therapeutically or prophylactically effective amount of the compound of claim 1 so that expression of ROCK 1 is inhibited.
 24. The method of claim 23 wherein the disease or condition is a hyperproliferative disorder. 